UNG (NM_003362) Human Untagged Clone

CAT#: SC109874

UNG (untagged)-Human uracil-DNA glycosylase (UNG), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UNG
• Synonyms: DGU; HIGM4; HIGM5; UDG; UNG1; UNG2; UNG15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003362, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCGTCTTCTGCCTTGGGCCGTGGGGGTTGGGCCGGAAGCTGCGGACGCCTGGGAAGGGGCCGCTGC
  AGCTCTTGAGCCGCCTCTGCGGGGACCACTTGCAGGCCATCCCAGCCAAGAAGGCCCCGGCTGGGCAGGA
  GGAGCCTGGGACGCCGCCCTCCTCGCCGCTGAGTGCCGAGCAGTTGGACCGGATCCAGAGGAACAAGGCC
  GCGGCCCTGCTCAGACTCGCGGCCCGCAACGTGCCCGTGGGCTTTGGAGAGAGCTGGAAGAAGCACCTCA
  GCGGGGAGTTCGGGAAACCGTATTTTATCAAGCTAATGGGATTTGTTGCAGAAGAAAGAAAGCATTACAC
  TGTTTATCCACCCCCACACCAAGTCTTCACCTGGACCCAGATGTGTGACATAAAAGATGTGAAGGTTGTC
  ATCCTGGGACAGGATCCATATCATGGACCTAATCAAGCTCACGGGCTCTGCTTTAGTGTTCAAAGGCCTG
  TTCCGCCTCCGCCCAGTTTGGAGAACATTTATAAAGAGTTGTCTACAGACATAGAGGATTTTGTTCATCC
  TGGCCATGGAGATTTATCTGGGTGGGCCAAGCAAGGTGTTCTCCTTCTCAACGCTGTCCTCACGGTTCGT
  GCCCATCAAGCCAACTCTCATAAGGAGCGAGGCTGGGAGCAGTTCACTGATGCAGTTGTGTCCTGGCTAA
  ATCAGAACTCGAATGGCCTTGTTTTCTTGCTCTGGGGCTCTTATGCTCAGAAGAAGGGCAGTGCCATTGA
  TAGGAAGCGGCACCATGTACTACAGACGGCTCATCCCTCCCCTTTGTCAGTGTATAGAGGGTTCTTTGGA
  TGTAGACACTTTTCAAAGACCAATGAGCTGCTGCAGAAGTCTGGCAAGAAGCCCATTGACTGGAAGGAGC
  TGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003362 unedited
  GGGTCAAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGTCACCGCG
  ACGCTCCTCGGGNAAGCCATAGGGCGCCTCCCAGCCCGTCTCCCCGCTCCAGTTTAGAAC
  CTAATTCCCAATTCCCGGAAGCTGCGGACGCCTGGGAAGGGGCCGCTGCAGCTCTTGAGC
  CGCCTCTGCGGGGACCACTTGCAGGCCATCCCAGCCAAGAAGGCCCCGGCTGGGCAGGAG
  GAGCCTGGGACGCCGCCCTCCTCGCCGCTGAGTGCCGAGCAGTTGGACCGGATCCAGAGG
  AACAAGGCCGCGGCCCTGCTCAGACTCGCGGCCCGCAACGTGCCCGTAGGCTTTGGAGAG
  AGCTGGAAGAAGCACCTCAGCGGGGAGTTCGAGAAACCGTATTTTATCAAGCTAATGGGA
  TTTGTTGCAGAAGAAAGAAAGCATTACACTGTTTATCCACCCCCACACCAAGTCTTCACC
  TGGACCCAGATGTGTGACATAAAAGATGTGAAGGTTGTCATCCTGGGACAGGATCCATAT
  CATGGACCTAATCAAGCTCACGGGCTCTGCTTTAGTGTTCAAAGGCCTGTTCCGCCTCCG
  CCCAGTTTGGAGAACATTTATAAAGAGTTGTCTACAGACATAGAGGATTTTGTTCATCCT
  GGCCATGGAGATTTATCTGGGTGGGCCAAGCAAGGTGTTCTCCTTCTCAACGCTGTCCTC
  ACGGTTCGTGCCCATCAAGCCAACTCTCATAAGGAGCGAGGCTGGGAGCAGTTCACTGAT
  GCAGTTGTGTCCTGGCTAAATCAGAACTCGAATGGGCCTNNGTTTTCTGCTCTGGGGCTC
  TTATGCTCAGAAGAAGGCAGTGCCATTGATAGGAAGCGGCACCATGTACTACAGACGGNT
  CATCCCTCCTTNTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003362 unedited
  NNNNTATCTCTGNAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTA
  ACTTTTAACAAACTTTTATTAACAAACCTCGCGTAGAGCCAACTCTAACCCGACTAACAG
  CAAAAACAGCATTGCTAACAAGATCAAGGATGGAGGTGCAGACCCAATAAAGACCGTCTC
  TTAAAAAAAGGACTGGGAAAAATATATATATATATATCTGCATGAGTACTTCTTAAAAAA
  CTAAAACTTTCCAGTGTTGCAGGAAAAAATATCCCAAAGTGATTTCAGTCGGTTAAAAAA
  AACAGTACTTGGCAGTCTGTGCTGCCTTAAAAACCCCAACTGGACATAACCCTAGGAGAA
  AATCACCCCACCCCATAAAGACAAAATTCCCTTTTCTCCTCTTCCTTGTTCAAATTCCAC
  TGCTTATTCCACCAACACCCAAGGAATAACAAGACCCAGCTTACTTCTCTCGGTCTCTTA
  TCTGATAAAGCTCACAGCAATCTTAACCTCTACTACAATCAGGAAATTGAGCCCTAATTT
  CTGGGCCAGATTCTGGAAAGTTGCAAAACATCCTTATGCTGCGAGGGGCCTGAGGACTCC
  TTCAAAGATCAAGGGGGCAGTGGAACTGGCAGAGACTGGCATGCCTGCCCTGCCAGGCTG
  AGACAAGGGGACCTTTCTAAGCAAAACACCCCCACATCTGTCACTGAAAGTCTAACCAGG
  CAAAACCAAGGAGCAACAGGCGCCCAAACTAGGCTGTGCCTGAAAAGTGCACATCTCCCC
  TGTTTCACCATAAAGGCGAGGGAAAAAAAAGAGCCAACTGAGTATTTGACCTCATTTGTC
  CTCTGCATAATTTAAGCCGAAACCATGTTGCAGAAAAAGACATTGGTCCAAAGGCACCCC
  TTTGTGGTNTGGGGGTGGAAACACTCGGCATTCN
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003362
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003362.2, NP_003353.1
• RefSeq Size: 2081 bp
• RefSeq ORF: 915 bp
• Locus ID: 7374
• Cytogenetics: 12q24.11
• Domains: UDG
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Primary immunodeficiency
• Gene Summary: 'This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (1) uses the downstream promoter and a full-length exon 1B. It encodes the UNG1 isoform, which includes a mitochondrial targeting sequence.