OGT (NM_181673) Human Untagged Clone

CAT#: SC109934

OGT (untagged)-Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OGT
• Synonyms: HINCUT-1; HRNT1; MRX106; O-GLCNAC; OGT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109934 sequence for NM_181673 edited (data generated by NextGen Sequencing)
  ATGGCGTCTTCCGTGGGCAACGTGGCCGACAGCACAGGGTTAGCTGAGTTGGCACATCGA
  GAATATCAGGCAGGAGATTTTGAGGCAGCTGAGAGACACTGCATGCAGCTCTGGAGACAA
  GAGCCAGACAATACTGGTGTGCTTTTATTACTTTCATCTATACACTTCCAGTGTCGAAGG
  CTGGACAGATCTGCTCACTTTAGCACTCTGGCAATTAAACAGAACCCCCTTCTGGCAGAA
  GCTTATTCGAATTTGGGGAATGTGTACAAGGAAAGAGGGCAGTTGCAGGAGGCAATTGAG
  CATTATCGACATGCATTGCGTCTCAAACCTGATTTCATCGATGGTTATATTAACCTGGCA
  GCCGCCTTGGTAGCAGCGGGTGACATGGAAGGGGCAGTACAAGCTTACGTCTCTGCTCTT
  CAGTACAATCCTGATTTGTACTGTGTTCGCAGTGACCTGGGGAACCTGCTCAAAGCCCTG
  GGTCGCTTGGAAGAAGCCAAGGCATGTTATTTGAAAGCAATTGAGACGCAACCGAACTTT
  GCAGTAGCTTGGAGTAATCTTGGCTGTGTTTTCAATGCACAAGGGGAAATTTGGCTTGCA
  ATTCATCACTTTGAAAAGGCTGTCACCCTTGACCCAAACTTTCTGGATGCTTATATCAAT
  TTAGGAAATGTCTTGAAAGAGGCACGCATTTTTGACAGAGCTGTGGCAGCTTATCTTCGT
  GCCCTAAGTTTGAGTCCAAATCACGCAGTGGTGCACGGCAACCTGGCTTGTGTATACTAT
  GAGCAAGGCCTGATAGATCTGGCAATAGACACCTACAGGCGGGCTATCGAACTACAACCA
  CATTTCCCTGATGCTTACTGCAACCTAGCCAATGCTCTCAAAGAGAAGGGCAGTGTTGCT
  GAAGCAGAAGATTGTTATAATACAGCTCTCCGTCTGTGTCCCACCCATGCAGACTCTCTG
  AATAACCTAGCCAATATCAAACGAGAACAGGGAAACATTGAAGAGGCAGTTCGCTTGTAT
  CGTAAAGCATTAGAAGTCTTCCCAGAGTTTGCTGCTGCCCATTCAAATTTAGCAAGTGTA
  CTGCAGCAGCAGGGAAAACTGCAGGAAGCTCTGATGCATTATAAGGAGGCTATTCGAATC
  AGTCCTACCTTTGCTGATGCCTACTCTAATATGGGAAACACTCTAAAGGAGATGCAGGAT
  GTTCAGGGAGCCTTGCAGTGTTATACGCGTGCCATCCAAATTAATCCTGCATTTGCAGAT
  GCACATAGCAATCTGGCTTCCATTCATAAGGATTCAGGGAATATTCCAGAAGCCATAGCT
  TCTTACCGCACGGCTCTGAAACTTAAGCCTGATTTTCCTGATGCTTATTGTAACTTGGCT
  CATTGCCTGCAGATTGTCTGTGATTGGACAGACTATGATGAGCGAATGAAGAAGTTGGTC
  AGTATTGTGGCTGACCAGTTAGAGAAGAATAGGTTGCCTTCTGTGCATCCTCATCATAGT
  ATGCTATATCCTCTTTCTCATGGCTTCAGGAAGGCTATTGCTGAGAGGCACGGCAACCTG
  TGCTTAGATAAGATTAATGTTCTTCATAAACCACCATATGAACATCCAAAAGACTTGAAG
  CTCAGTGATGGTCGGCTGCGTGTAGGATATGTGAGTTCCGACTTTGGGAATCATCCTACT
  TCTCACCTTATGCAGTCTATTCCAGGCATGCACAATCCTGATAAATTTGAGGTGTTCTGT
  TATGCCCTGAGCCCAGACGATGGCACAAACTTCCGAGTGAAGGTGATGGCAGAAGCCAAT
  CATTTCATTGATCTTTCTCAGATTCCATGCAATGGAAAAGCAGCTGATCGCATCCATCAG
  GATGGAATTCATATCCTTGTAAATATGAATGGCTATACTAAGGGCGCTCGAAATGAGCTT
  TTTGCTCTCAGGCCAGCTCCTATTCAGGCAATGTGGCTGGGATACCCTGGGACGAGTGGT
  GCGCTTTTCATGGATTATATTATCACTGATCAGGAAACTTCGCCAGCTGAAGTTGCTGAG
  CAGTATTCCGAGAAATTGGCTTATATGCCCCACACTTTTTTTATTGGTGATCATGCTAAT
  ATGTTCCCTCACCTGAAGAAAAAAGCAGTCATCGATTTTAAGTCCAATGGGCACATTTAT
  GACAATCGGATAGTTCTGAATGGCATCGACCTCAAAGCATTTCTTGATAGTCTACCAGAT
  GTGAAAATTGTCAAGATGAAGTGTCCTGATGGAGGAGACAATGCAGATAGCAGTAACACA
  GCTCTTAATATGCCTGTTATTCCTATGAATACTATTGCAGAAGCAGTTATTGAAATGATT
  AACCGAGGACAGATTCAAATAACAATTAATGGATTCAGTATTAGCAATGGACTGGCAACT
  ACTCAGATCAACAATAAGGCTGCAACTGGAGAGGAGGTTCCCCGTACCATTATTGTAACC
  ACCCGTTCTCAGTACGGGTTACCAGAAGATGCCATCGTATACTGTAACTTTAATCAGTTG
  TATAAAATTGACCCTTCTACTTTGCAGATGTGGGCAAACATTCTGAAGCGTGTTCCCAAT
  AGTGTACTCTGGCTGTTGCGTTTTCCAGCAGTAGGAGAACCTAATATTCAACAGTATGCA
  CAAAACATGGGCCTGCCCCAGAACCGTATCATTTTTTCACCTGTTGCTCCTAAAGAGGAA
  CACGTCAGGAGAGGCCAGCTGGCTGATGTCTGCTTGGACACTCCACTCTGTAATGGGCAC
  ACCACAGGGATGGATGTCCTCTGGGCAGGGACCCCCATGGTGACTATGCCAGGAGAGACT
  CTTGCTTCTCGAGTTGCAGCATCCCAGCTCACTTGCTTAGGTTGTCTTGAGCTTATTGCT
  AAAAACAGACAAGAATATGAAGACATAGCTGTGAAGCTGGGAACTGATCTAGAATACCTG
  AAGAAAGTTCGTGGCAAAGTCTGGAAGCAAAGAATATCTAGCCCTCTGTTCAACACCAAA
  CAATACACAATGGAACTAGAGCGGCTCTATCTACAGATGTGGGAGCATTATGCAGCTGGC
  AACAAACCTGACCACATGATTAAGCCTGTTGAAGTCACTGAGTCAGCATAA
  
  Clone variation with respect to NM_181673.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181673 unedited
  CCGCGNNCCCGGNNCNCNCCCCCNCCTTTTNNNNNNCNCCCCGTTCAGAAATTGTNATAC
  CACTCACTNATAGGCGGCCGCGNAATTCGCACGAGNAGAGGTCAATTAGAGTTCCCAGGG
  TTTGAAGCCTGTAACTGCTGCCGCCGCTCAAGCCCTCCAGAGCATTGCTACGGCTGCTGC
  CCTTGTACTACTACCTCCAAATACGTTCTTGCTGGTAGTGGCGGCAGCAGGACCAATTAC
  CTCTTTTTGCTCTCCCTCGAGAAGCTCCAGATGGCGTCTTCCGTGGGCAACGTGGCCGAC
  AGCACAGGGTTAGCTGAGTTGGCACATCGAGAATATCAGGCAGGAGATTTTGAGGCAGCT
  GAGAGACACTGCATGCAGCTCTGGAGACAAGAGCCAGACAATACTGGTGTGCTTTTATTA
  CTTTCATCTATACACTTCCAGTGTCGAAGGCTGGACAGATCTGCTCACTTTAGCACTCTG
  GCAATTAAACAGAACCCCCTTCTGGCAGAAGCTTATTCGAATTTGGGGAATGTGTACAAG
  GAAAGAGGGCAGTTGCAGGAGGCAATTGAGCATTATCGACATGCATTGCGTCTCAAACCT
  GATTTCATCGATGGTTATATTAACCTGGCAGCCGCCTTGGTAGCAGCGGGTGACATGGAA
  GGGGCAGTACAAGCTTACGTCTCTGCTCTTCAGTACAATCCTGATTTGTACTGTGTTCGC
  AGTGACCTGNGGAACCTGCTCAAAGCCCTGNGTCGCTTGGAAGAGCCCAAGCATGTTATT
  TGAAAGCAATTGAGACGCACCCGAACTTTGCAGTAGCCTTGGAGTATCTGGGCTGTGTTN
  TTAATGCACAAGGGGAAATTTGCCTTGCAATTCATCACTTTGNAAAGGCCTGTCACCCTT
  GACCCAAACTTTTCTGGAGCTTATATCAATTTAGAAAAGTCTTGAAAGAGCACGCATTTT
  TGACAGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003605 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGCTCAAGTGGTTTATAAAACCTA
  TAAAAAGACTACACCAGCAAAGTCCCTGTCAATCTGTCAGAGTTCAGAAACTAAAACAGG
  GAGTAACATTTTAGCTTAAAACCTTATCTCAAGAGAATCATATACACTTCACATGAATAA
  AAATACCTGAAACCAAACATTTTTAAAAGCTCCAGTACCCAAAATATAAAGAAAAAAAAA
  TCCAGAAGACTGAATCAATCCATGGAATCACAAAGCGGCCGGACAATCTATATCTCCCCT
  CCACACTAACCATCCCATGGAAGACCAAGGGAGATCAGACCTTCCAGACCTATGCACCAT
  CTGGTCGCCGCAAAATTCTACGGAGATTCCTTGTGGAAAAGCAGTTTCCCATCTTTTGTG
  TCTCTCCCTACCATGCAGGAAGCAAGTCTGGCTGTGCTATTCTATTACCATTATATATCA
  CCCATCTGCAACAAGGTACTGTACAGACAAGTAAGAAGTATGTTATCTAGTTCCCTTTCC
  CCCAGAAGGTTGAGGCTCAGGTATAGGGGTAATTCTCCTGTGCAGTCTTTATTTATGCTG
  ACTCAGTGACTTCAACAGGCTTAATCATGTGGTCAGGTTTGTTGCCAGCTGCATAATGCT
  CCCACATCTGTAGATAGAGCCGCTCTAGNTCCATTGGGTATTGTTTGGGGGTGAACAGAG
  GGCTAGATATTCTTTGCTTCCAGACTTTGCCACGAACTTTCTTCAGGTATTCTAGATCAG
  TTCCCAGCTTAACAGCATGCCTCATATTCTGGGCTGTTTTAGCATAAGCCAAGACAACTA
  GCAGTGAGCTGGATGCGCACCTCAGAACAGAGCTTTCTGCATATCCCATGGGGTCCTGCC
  AGAGACATCTCCTGGGGGGCCCTACAGAGGGAGGTCAACAAATAGCAGTGCCTTCTGGAG
  GTCTCTAGGCCAAGGAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181673
• ORF Size: 3111 bp
• Insert Size: 4000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_181673.1, NP_858059.1
• RefSeq Size: 5445
• RefSeq ORF: 3111
• Locus ID: 8473
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1.