Endothelin 3 (EDN3) (NM_000114) Human Untagged Clone

CAT#: SC110001

EDN3 (untagged)-Human endothelin 3 (EDN3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EDN3
• Synonyms: ET-3; ET3; HSCR4; PPET3; WS4B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110001 sequence for NM_000114 edited (data generated by NextGen Sequencing)
  ATGGAGCCGGGGCTGTGGCTCCTTTTCGGGCTCACAGTGACCTCCGCCGCAGGATTCGTG
  CCTTGCTCCCAGTCTGGGGATGCTGGCAGGCGCGGCGTGTCCCAGGCCCCCACTGCAGCC
  AGATCTGAGGGGGACTGTGAAGAGACTGTGGCTGGCCCTGGCGAGGAGACTGTGGCTGGC
  CCTGGCGAGGGGACTGTGGCCCCGACAGCACTGCAGGGTCCAAGCCCTGGAAGCCCTGGG
  CAGGAGCAGGCGGCCGAGGGGGCCCCTGAGCACCACCGATCCAGGCGCTGCACGTGCTTC
  ACCTACAAGGACAAGGAGTGTGTCTACTATTGCCACCTGGACATCATTTGGATCAACACT
  CCCGAACAGACGGTGCCCTATGGACTGTCCAACTACAGAGGAAGCTTCCGGGGCAAGAGG
  TCTGCGGGGCCACTTCCAGGGAATCTGCAGCTCTCACATCGGCCACACTTGCGCTGCGCT
  TGTGTGGGGAGATATGACAAGGCCTGCCTGCACTTTTGCACCCAAACTCTGGACGTCAGC
  AGTAATTCAAGGACGGCAGAAAAAACAGACAAAGAAGAGGAAGGGAAGGTTGAAGTCAAG
  GACCAACAAAGCAAGCAGGCTTTAGACCTCCACCATCCAAAGCTCATGCCCGGCAGTGGA
  CTCGCCCTCGCTCCATCTACCTGCCCCCGCTGCCTCTTTCAGGAAGGAGCCCCTTAG
  
  Clone variation with respect to NM_000114.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000114 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGTCCTCCTGGTCCGG
  TGCTCCGGCGCCTGATCTAGGTTCATGGAGCCGGGGCTGTGGCTCCTTTTCGGGCTCACA
  GTGACCTCCGCCGCAGGATTCGTGCCTTGCTCCCAGTCTGGGGATGCTGGCAGGCGCGGC
  GTGTCCCAGGCCCCCACTGCAGCCAGATCTGAGGGGGACTGTGAAGAGACTGTGGCTGGC
  CCTGGCGAGGAGACTGTGGCTGGCCCTGGCGAGGGGACTGTGGCCCCGACAGCACTGCAG
  GGTCCAAGCCCTGGAAGCCCTGGGCAGGAGCAGGCGGCCGAGGGGGCCCCTGAGCACCAC
  CGATCCAGGCGCTGCACGTGCTTCACCTACAAGGACAAGGAGTGTGTCTACTATTGCCAC
  CTGGACATCATTTGGATCAACACTCCCGAACAGACGGTGCCCTATGGACTGTCCAACTAC
  AGAGGAAGCTTCCGGGGCAAGAGGTCTGCGGGGCCACTTCCAGGGAATCTGCAGCTCTCA
  CATCGGCCACACTTGCGCTGCGCTTGTGTGGGGAGATATGACAAGGCCTGCCTGCACTTT
  TGCACCCAAACTCTGGACGTCAGCAGTAATTCAAGGACGGCAGAAAAAACAGACAAAGAA
  GAGGAAGGGAAGACAGGTTGAAGTCAAGGACCAACATAGCAAGCAGGCTNNTAGACCTNC
  ACCATCCAAAGCTCATGCCCGGCAGTGGACTCGCCCTCGCTCCATCTACCTGCCCNCGCT
  GCCTCTTNCAAGGAAGGAGCCNNCTTAGAGGACAGGCCTGCAGCATCCTGGTCTCGGGGA
  GGCTTCTGTCATTGCTCACAACAGTTCAGATTTCCACTCTTTTAGACAGAAGTGATTGCT
  GGGCGCGGCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000114 unedited
  TAGCTTTGGACCGGCGGCCGCAATCTAGGATCGAGGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTCAAAACTTTAGGTTTTTTATTAGGCTTTTGACAGTACTTTCTGAGTATTTGAGCAAT
  TGAGGGGTTTCTCTTTGATACCATCTTGCCACGGGGCCAAACAGCACATATGCATTTTAA
  AAATGTAATTCAAGCTCTAAGGAGTATGAGTTTGTATTCAAAATGCAATTTAAAATCTAC
  CTACCTATCTACCTCTTTATACGTATATACAGGGACTACTTTGAACAGATCCACATAAAG
  GAGTTGGAGGGGAAAACAGTTTCACAATAAATAATCGTTTCTTTAAACTGTACAAAATCC
  TACCAGCAAATCAGCCACTTTCATTTCCTCATGATAGGTCATCACTTTTATACCTGTAAT
  ACCTTATGAAATACACACAGAGGATTTAACATTTGGTCAAGGCCAATGCTCTGTCATACA
  CCATCTTAAATGTCTCTCTACGGGGCCCTGGACACCCACTCAAATGCCGTTTCCTATCTC
  TCTGTGACTGGGTTGGGGAGATGGGCAGCCCTCCCCTGCGCAGGCGACTTTACATGGGGG
  GATAACATCTCCACACTCACAGTAAGCTCGGCTCGGTGGGAAAAGCGCAGCTGAAAGAGA
  TTTTGAACTGTCAAACGGTATAAGTTGTTGCCAATTTATATAAACAGGAATTTACATAAT
  TTACAAAGAATTTGTGTTCGTGACTTATTTTGAAGCCATTCCCCAAAGATCCACATTTAC
  TCATGAAAGCAAATATTCAACCTAATAGCCTTGAGCTGNGGCTGTCTTTAAGTCGGCTCC
  TGCAAACAGGCTGNGAACTCACTGTGTATCGCCTGTGCCTATCAGATACAGAGGTCAGCC
  CTGACAGAACAGCCTGNATGCAGTCTACTTCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000114
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000114.2, NP_000105.1
• RefSeq Size: 2438 bp
• RefSeq ORF: 717 bp
• Locus ID: 1908
• Cytogenetics: 20q13.32
• Domains: END
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).