MEK2 (MAP2K2) (NM_030662) Human Untagged Clone

CAT#: SC110003

MAP2K2 (untagged)-Human mitogen-activated protein kinase kinase 2 (MAP2K2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP2K2
• Synonyms: CFC4; MAPKK2; MEK2; MKK2; PRKMK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_030662 edited
  ATGCTGGCCCGGAGGAAGCCGGTGCTGCCGGCGCTCACCATCAACCCTACCATCGCCGAG
  GGCCCATCCCCTACCAGCGAGGGCGCCTCCGAGGCAAACCTGGTGGACCTGCAGAAGAAG
  CTGGAGGAGCTGGAACTTGACGAGCAGCAGAAGAAGCGGCTGGAAGCCTTTCTCACCCAG
  AAAGCCAAGGTCGGCGAACTCAAAGACGATGACTTCGAAAGGATCTCAGAGCTGGGCGCG
  GGCAACGGCGGGGTGGTCACCAAAGTCCAGCACAGACCCTCGGGCCTCATCATGGCCAGG
  AAGCTGATCCACCTTGAGATCAAGCCGGCCATCCGGAACCAGATCATCCGCGAGCTGCAG
  GTCCTGCACGAATGCAACTCGCCGTACATCGTGGGCTTCTACGGGGCCTTCTACAGTGAC
  GGGGAGATCAGCATTTGCATGGAACACATGGACGGCGGCTCCCTGGACCAGGTGCTGAAA
  GAGGCCAAGAGGATTCCCGAGGAGATCCTGGGGAAAGTCAGCATCGCGGTTCTCCGGGGC
  TTGGCGTACCTCCGAGAGAAGCACCAGATCATGCACCGAGATGTGAAGCCCTCCAACATC
  CTCGTGAACTCTAGAGGGGAGATCAAGCTGTGTGACTTCGGGGTGAGCGGCCAGCTCATC
  GACTCCATGGCCAACTCCTTCGTGGGCACGCGCTCCTACATGGCTCCGGAGCGGTTGCAG
  GGCACACATTACTCGGTGCAGTCGGACATCTGGAGCATGGGCCTGTCCCTGGTGGAGCTG
  GCCGTCGGAAGGTACCCCATCCCCCCGCCCGACGCCAAAGAGCTGGAGGCCATCTTTGGC
  CGGCCCGTGGTCGACGGGGAAGAAGGAGAGCCTCACAGCATCTCGCCTCGGCCGAGGCCC
  CCCGGGCGCCCCGTCAGCGGTCACGGGATGGATAGCCGGCCTGCCATGGCCATCTTTGAA
  CTCCTGGACTATATTGTGAACGAGCCACCTCCTAAGCTGCCCAACGGTGTGTTCACCCCC
  GACTTCCAGGAGTTTGTCAATAAATGCCTCATCAAGAACCCAGCGGAGCGGGCGGACCTG
  AAGATGCTCACAAACCACACCTTCATCAAGCGGTCCGAGGTGGAAGAAGTGGATTTTGCC
  GGCTGGTTGTGTAAAACCCTGCGGCTGAACCAGCCCGGCACACCCACGCGCACCGCCGTG
  TGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030662 unedited
  GGGTACCGTTCAAAATTGTATACGACTCATATGGGCGGCCGCGNAATTCGCACNGGGGCT
  GCGGCGTCAGCCTTCTTCGGGCCTCGGCAGCGGTAGCGGCTCGCTCGCCTCAGCCCCAGC
  GCCCCTCGGCTACCCTCGGCCCAGGCCCGCAGCGCCGCCCGCCCTCGGCCGCCCCGACGC
  CGGCCTGGGCCGCGGCCGCAGCCCCGGGCTCGCGTAGGCGCCGACCGCTCCCGGCCCGCC
  CCCTATGGGCCCCGGCTAGAGGCGCCGCCGCCGCCGGCCCGCGGAGCCCCGATGCTGGCC
  CGGAGGAAGCCGGTGCTGCCGGCGCTCACCATCAACCCTACCATCGCCGAGGGCCCATCC
  CCTACCAGCGAGGGCGCCTCCGAGGCAAACCTGGTGGACCTGCAGAAGAAGCTGGAGGAG
  CTGGAACTTGACGAGCAGCAGAAGAAGCGGCTGGAAGCCTTTCTCACCCAGAAAGCCAAG
  GTCGGCGAACTCAAAGACGATGACTTCGAAAGGATCTCAGAGCTGGGCGCGGGCAACGGC
  GGGGTGGTCACCAAAGTCCAGCACAGACCCTCGGGCCTCATCATGGCCAGGAAGCTGATC
  CACCTTGAGATCAAGCCGGCCATCCGGAACCAGATCATCCGCGAGCTGCAGGTCCTGCAC
  GAATGCAACTCGCCGTACATCGTGGGCTTCTACGGGGCCTTCTACAGTGACGGGGAGATC
  AGCATTTGCATGGAACACATGGACGGCGGCTCCCTGGACCAGGTGCTGAAAGAGGCCAAG
  AGGATTCCCGAGGAGATCCTGGGGAAAGTCAGCATCGCGGTTCTCCGGGGCTTGGCGTAC
  CTCCGAGAGAAGCACCAGATCATGCACCGAGATGTGAAGCCCTCCNACATCCTCGTGAAC
  TCTAGAGGGAAGATCAAGCTGTGTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030662 unedited
  ACCGCGGCCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCTTTTTCTCTCCCTG
  TTTTGTCTTTGTAACCCTAAGGGAAGCAGCAGCCTCTTGAAACCACACACAGCAGCGTCG
  CCCGTCCCCAGAGGCACCCCGGCCAGGACGGGCAGGAGAGGAGACCCCCGTTCCTGCATG
  CGCTGTCGCCCCGCCACGGTGCTCTCCGCAGGGGTGAGGCAGGAGGGTGGGTGGAGGCGC
  CAGCCTGTCCTCAGCTGGAAGGGCGGGGCATGGACAGGGACGGTGGGCAGGTCACCAGCA
  GGACGCAGGGAGCCCGGCCACTGTCACACGGCGGTGCGCGTGGGTGTGCCGGGCTGGCTC
  ACCCGCAGGGTTTTACACAACCAGCCGGCAAAATCCACTTCTTCCACCTCGGACCGCTTG
  ATGAAGGTGTGGTTTGTGAGCATCTTCAGGCCCGCCCGCTCCGCTGGGTTCTTGATGAGG
  CATTTATTGACAAACTCCTGGAAGTCGGGGGTGAACACCCCGCCGGGCAACTTAGGAGGT
  GGTCCGCTTCCACTATTATCCAGGAGCCTCAAAGATGGTCATGGCTGGCCGGCTATCCAT
  TCCGTGACCGCTGACGGGGCGCCCGGGGGGCCCTTGATCGGGTTAGATGCTGTGAGGCTC
  TACCTTTTTACTCGTCTTCCCCCGCCCTGTTCTAAAACGGACTCTCCCCCTTTTGCCGCC
  TAGTCGGGGGGATTGGGTATCCTCCCTCGGGCTACTCCTCCCCGGTTCCGGTCTCTGCCT
  TTCTATGTTCTACTGTCCCCCCTCTATGGGTGCCTTGATCCCGCTATTGTACCTTTTCTC
  GAATCCTCCGCCTCATTAGGTATTCGTACTTCGATCTACTATAACTGGATCCCTACTCTC
  ACTAACTTCTCCCCTTTTCTTCTCCCCTCTCATACCCCCGAGGTTGTTGTCCGGGCTCCC
  N
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030662
• Insert Size: 1810 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030662.2, NP_109587.1
• RefSeq Size: 1759 bp
• RefSeq ORF: 1203 bp
• Locus ID: 5605
• Cytogenetics: 19p13.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chronic myeloid leukemia, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prion diseases, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: 'The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]'