MBD2 (NM_015832) Human Untagged Clone

CAT#: SC110013

MBD2 (untagged)-Human methyl-CpG binding domain protein 2 (MBD2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MBD2
• Synonyms: DMTase; NY-CO-41
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015832 edited
  GCGCGCCGCGCTGCGGGCGGGGCGGGTCTCCGGGATTCCAAGGGCTCGGTTACGGAAGAA
  GCGCAGCGCCGGCTGGGGAGGGGGCTGGATGCGCGCGCACCCGGGGGGAGGCCGCTGCTG
  CCCGGAGCAGGAGGAGGGGGAGAGTGCGGCGGGCGGCAGCGGCGCTGGCGGCGACTCCGC
  CATAGAGCAGGGGGGCCAGGGCAGCGCGCTCGCCCCGTCCCCGGTGAGCGGCGTGCGCAG
  GGAAGGCGCTCGGGGCGGCGGCCGTGGCCGGGGGCGGTGGAAGCAGGCGGGCCGGGGCGG
  CGGCGTCTGTGGCCGTGGCCGGGGCCGGGGCCGTGGCCGGGGACGGGGACGGGGCCGGGG
  CCGGGGCCGCGGCCGTCCCCCGAGTGGCGGCAGCGGCCTTGGCGGCGACGGCGGCGGCTG
  CGGCGGCGGCGGCAGCGGTGGCGGCGGCGCCCCCCGGCGGGAGCCGGTCCCTTTCCCGTC
  GGGGAGCGCGGGGCCGGGGCCCAGGGGACCCCGGGCCACGGAGAGCGGGAAGAGGATGGA
  TTGCCCGGCCCTCCCCCCCGGATGGAAGAAGGAGGAAGTGATCCGAAAATCTGGGCTAAG
  TGCTGGCAAGAGCGATGTCTACTACTTCAGTCCAAGTGGTAAGAAGTTCAGAAGCAAGCC
  TCAGTTGGCAAGGTACCTGGGAAATACTGTTGATCTCAGCAGTTTTGACTTCAGAACTGG
  AAAGATGATGCCTAGTAAATTACAGAAGAACAAACAGAGACTGCGAAACGATCCTCTCAA
  TCAAAATAAGCTGCGCTGGAACACTCATCGTCCTGCACCATGGCATGCGCTTTCAAGACT
  CTGCTTGCTCATACGCTGTTTGCTCTGCTTGGAATGTGCTTACCCCCTTCCCCTTCATCT
  GGTGAACTCCTACTCATCCAAGACCCAGCTTCATTGTCTCCATCTCTGGGAAGCCTGCCC
  TGCATACTCCAGGCAGAACCAATCCTTTCCTCCATAATCTAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015832
• ORF Size: 909 bp
• Insert Size: 1000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_015832.3.

Reference Data

• RefSeq: NM_015832.3, NP_056647.1
• RefSeq Size: 1357
• RefSeq ORF: 909
• Locus ID: 8932
• Domains: MBD
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region, compared to variant 1, resulting in a shorter protein (testis-specific) that has a shorter C-terminus, compared to isoform 1.