NFS1 (NM_021100) Human Untagged Clone

CAT#: SC110023

NFS1 (untagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFS1
• Synonyms: HUSSY-08; IscS; NIFS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021100, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCTCCGAGCCGCTTGGAGGCGGGCGGCAGTGGCGGTGACAGCGGCTCCAGGGCCGAAGCCCGCGG
  CGCCCACTCGGGGGCTGCGCCTGCGCGTTGGAGACCGTGCTCCTCAGTCTGCGGTTCCCGCAGATACAGC
  CGCTGCCCCGGAGGTGGGGCCAGTGCTGCGACCTCTCTATATGGATGTGCAAGCTACAACTCCTCTGGAC
  CCCCGGGTGCTTGATGCCATGCTCCCTTACCTAATCAACTACTATGGGAACCCACACTCCCGGACACATG
  CTTATGGCTGGGAGAGTGAGGCAGCCATGGAACGTGCTCGTCAGCAAGTAGCATCTCTGATTGGAGCTGA
  TCCTCGTGAGATCATTTTTACTAGTGGTGCTACTGAATCCAACAACATAGCAATTAAGGGGGTGGCCCGA
  TTCTACAGGTCACGGAAAAAGCACTTGATCACCACCCAGACAGAACACAAATGTGTCTTGGACTCCTGCC
  GTTCACTGGAAGCTGAGGGCTTTCAGGTCACCTACCTCCCAGTGCAGAAGAGTGGGATCATTGACCTAAA
  GGAACTAGAGGCTGCTATCCAGCCAGATACTAGCCTGGTGTCAGTCATGACTGTGAACAATGAGATTGGA
  GTGAAGCAGCCTATTGCAGAAATAGGGCGGATTTGCAGTTCCAGAAAGGTATATTTCCATACTGATGCAG
  CCCAGGCTGTTGGAAAAATCCCACTTGATGTCAATGACATGAAAATTGATCTCATGAGCATTAGTGGTCA
  CAAAATCTACGGTCCCAAAGGGGTTGGTGCCATCTACATCCGTCGCCGGCCCCGTGTGCGTGTGGAGGCC
  CTGCAGAGTGGAGGGGGGCAGGAGCGGGGTATGCGGTCTGGGACAGTGCCCACACCCTTAGTGGTGGGGC
  TGGGGGCTGCGTGTGAGGTGGCACAGCAAGAGATGGAGTATGACCACAAGCGAATCTCAAAGTTGTCAGA
  GCGGCTGATACAGAATATAATGAAGAGCCTTCCAGATGTGGTGATGAATGGGGACCCTAAGCACCATTAT
  CCCGGCTGTATCAACCTCTCCTTTGCATATGTGGAAGGGGAAAGTCTGCTGATGGCACTGAAGGACGTTG
  CCTTATCCTCAGGGAGTGCCTGCACCTCTGCATCCCTGGAGCCCTCTTATGTGCTTAGAGCAATTGGCAC
  TGATGAGGATTTAGCGCACTCTTCTATCAGGTTTGGAATTGGCCGCTTCACTACAGAGGAGGAAGTGGAC
  TACACAGTGGAGAAATGCATTCAGCATGTGAAGCGTCTTCGAGAAATGAGCCCTCTCTGGGAGATGGTTC
  AGGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAACACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021100 unedited
  GTCATATTTGTATACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGGTGGGCTCTGC
  CAGCGGGACCATGCTGCTCCGAGCCGCTTGGAGGCGGGCGGCAGTGGCGGTGACAGCGGC
  TCCAGGGCCGAAGCCCGCGGCGCCCACTCGGGGGCTGCGCCTGCGCGTTGGAGACCGTGC
  TCCTCAGTCTGCGGTTCCCGCAGATACAGCCGCTGCCCCGGAGGTGGGGCCAGTGCTGCG
  ACCTCTCTATATGGATGTGCAAGCTACAACTCCTCTGGACCCCCGGGTGCTTGATGCCAT
  GCTCCCTTACCTAATCAACTACTATGGGAACCCACACTCCCGGACACATGCTTATGGCTG
  GGAGAGTGAGGCAGCCATGGAACGTGCTCGTCAGCAAGTAGCATCTCTGATTGGAGCTGA
  TCCTCGTGAGATCATTTTTACTAGTGGTGCTACTGAATCCAACAACATAGCAATTAAGGG
  GGTGGCCCGATTCTACAGGTCACGGAAAAAGCACTTGATCACCACCCAGACAGAACACAA
  ATGTGTCTTGGACTCCTGCCGTTCACTGGAAGCTGAGGGCTTTCAGGTCACCTACCTCCC
  AGTGCAGAAGAGTGGGATCATTGACCTAAAGGAACTAGAGGCTGCTATCCAGCCAGATAC
  TAGCCTGGTGTCAGTCATGACTGTGAACAATGAGATTGGAGTGAAGCAGCCTATTGCAGA
  AATAGGGCGGATTTGCAGTTCCAGAAAGGTATATTTCCATACTGATGCAGCCCAGGCTGT
  TGGAAAAATCCACTTGATGTCAATGACATGAAAATTGATCTCATGAGCATAAGTGGTCAC
  CAAATCTACGGTCCCCAAGGGGTTTGGTGCCATCTACATNCGTCGCCGCCCCCGTGTGCG
  TTGTGGAGCCTTGCACAGTGAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021100 unedited
  CGGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAAATAAAAGGTGAGTTTATT
  TCTCCAGTCTTGAAATTAACAATTTTCAAGAAAACAAATTATTAGAATAACTGGTGGAGT
  CACAGACATATTTCTAGCTCCATTAGGTCAAAGGAAAGGAAAGAGGACAAGGGTAGAAAG
  GAGACAAGCCCTCTAAGAAACTGTCCATTCAGTCTGTCTGCAGTCAATATGAAGAGATAG
  CCTTTGGAGCTACAGAAAATATTACATTGAAGTGGATTATGCTTGCTGTTAAAGAAGCTG
  GTCCAGCCGAGCAGCAGCAGAAGACGAAGACTCAAATGTCCATGTAGAGCATCATGGTCC
  CTGACCAAAGAGACTTCTTCTGGTTCCTCTTGCTCTCCTCACCAAGAGCACAGATGTGCC
  CAGCAGCAATATGACAGCAAATGTCTCTATGGCTTCGGGATGAAAATGTCCACACACTTT
  AAGACCCGAGAAGAAATGGGGAGTGCTCCACACCCAAGGAACTGAAGCTAGGGAAAGACA
  GTTTTCTTGTGTTTCTGTCATAGAGGTGGACTGATGCTGAAGTCAACTGGTCTATACCAA
  TCTAGAGCATCCACTAGGTGTAACAAGGTGTCTGGTTGTGCACGGGTTGGTGAGGCAGGA
  GGGGCCANACCAGCACAAAGTCAGGGCCCTATTCTTCTAGTGTTGGGTCCACTTGATGCT
  CTAGAGGTCAATGCCATCCTGAACCATCTCCCGAGAGGGCTCATTTCTCGAAGACGCTTT
  CACATGCTGATGCATTCCTCCACTGGGTAGTCCACTTCCTCCTCTGTAGTGAAGCGGGCA
  ATTCCAACCTGATAGAAGATGCGCTAAATCTCATAGTGGCCATTGCTCTAGCCCATAGAG
  GCCTCAGGATGCANAGTGCGAGCACNCCTGAGATAAGGCACCGNCCTCATGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021100
• ORF Size: 1374 bp
• Insert Size: 2440
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_021100.3, NP_066923.3
• RefSeq Size: 2371
• RefSeq ORF: 1374
• Locus ID: 9054
• Domains: aminotran_5
• Protein Pathways: Thiamine metabolism
• Gene Summary: Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) encodes the longer isoform (a).