NFS1 (NM_021100) Human Untagged Clone

CAT#: SC322710

NFS1 (untagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1


  "NM_021100" in other vectors (7)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NFS1
Synonyms HUSSY-08; IscS; NIFS
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for SC322710 GGGCCCAGGACTGCAGCGGCTTCGGAAGGTGGGCTCTGCCAGCGGGACCATGCTGCTCCG
AGCCGCTTGGAGGCGGGCGGCAGTGGCGGTGACAGCGGCTCCAGGGCCGAAGCCCGCGGC
GCCCACTCGGGGGCTGCGCCTGCGCGTTGGAGACCGTGCTCCTCAGTCTGCGGTTCCCGC
AGATACAGCCGCTGCCCCGGAGGTGGGGCCAGTGCTGCGACCTCTCTATATGGATGTGCA
AGCTACAACTCCTCTGGACCCCCGGGTGCTTGATGCCATGCTCCCTTACCTAATTAACTA
CTATGGGAACCCACACTCCCGGACACATGCTTATGGCTGGGAGAGTGAGGCAGCCATGGA
ACGTGCTCGTCAGCAAGTAGCATCTCTGATTGGAGCTGATCCTCGTGAGATCATTTTTAC
TAGTGGTGCTACTGAATCCAACAACATAGCAATTAAGGGGGTGGCCCGATTCTACAGGTC
ACGGAAAAAGCACTTGATCACCACCCAGACAGAACACAAATGTGTCTTGGACTCCTGCCG
TTCACTGGAAGCTGAGGGCTTTCAGGTCACCTACCTCCCAGTGCAGAAGAGTGGGATCAT
TGACCTAAAGGAACTAGAGGCTGCTATCCAGCCAGATACTAGCCTGGTGTCAGTCATGAC
TGTGAACAATGAGATTGGAGTGAAGCAGCCTATTGCAGAAATAGGGCGGATTTGCAGTTC
CAGAAAGGTATATTTCCATACTGATGCAGCCCAGGCTGTTGGAAAAATCCCACTTGATGT
CAATGACATGAAAATTGATCTCATGAGCATTAGTGGTCACAAAATCTACGGTCCCAAAGG
GGTTGGTGCCATCTACATCCGTCGCCGGCCCCGTGTGCGTGTGGAGGCCCTGCAGAGTGG
AGGGGGGCAGGAGCGGGGTATGCGGTCTGGGACAGTGCCCACACCCTTAGTGGTGGGGCT
GGGGGCTGCGTGTGAGGTGGCACAGCAAGAGATGGAGTATGACCACAAGCGAATCTCAAA
GTTGTCAGAGCGGCTGATACAGAATATAATGAAGAGCCTTCCAGATGTGGTGATGAATGG
GGACCCTAAGCACCATTATCCCGGCTGTATCAACCTCTCCTTTGCATATGTGGAAGGGGA
AAGTCTGCTGATGGCACTGAAGGACGTTGCCTTATCCTCAGGGAGTGCCTGCACCTCTGC
ATCCCTGGAGCCCTCTTATGTGCTTAGAGCAATTGGCACTGATGAGGATTTAGCGCACTC
TTCTATCAGGTTTGGAATTGGCCGCTTCACTACAGAGGAGGAAGTGGACTACACAGTGGA
GAAATGCATTCAGCATGTGAAGCGTCTTCGAGAAATGAGCCCTCTCTGGGAGATGGTTCA
GGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAACACTAGAAGAATAGGGCCCTGAC
TTTGTGCTGGTCTGGCCCCTCCTGCCTCACCAACCCGTGCACAACCAGACACCTTGTTAC
ACCTAGTGGATGCTCTAGATTGGTATAGACCAGTTGACTTCAGCATCAGTCCACCTCTAT
GACAGAAACACAAGAAAACTGTCTTTCCCTAGCTTCAGTTCCTTGGGTGTGGAGCACTCC
CCATTTCTTCTCGGGTCTTAAAGTGTGTGGACATTTTCATCCCGAAGCCATAGAGACATT
TGCTGTCATATTGCTGCTGGGCACATCTGTGCTCTTGGTGAGGAGAGCAAGAGGAACCAG
AAGAAGTCTCTTTGGTCAGGGACCATGATGCTCTACATGGACATTTGAGTCTTCGTCTTC
TGCTGCTGCTCGGCTGGACCAGCTTCTTTAACAGCAAGCATAATCCACTTCAATGTAATA
TTTTCTGTAGCTCCAAAGGCTATCTCTTCATATTGACTGCAGACAGACTGAATGGACAGT
TTCTTAGAGGGCTTGTCTCCTTTCTACCCTTGTCCTCTTTCCTTTCCTTTGACCTAATGG
AGCTAGAAATATGTCTGTGACTCCACCAGTTATTCTAATAATTTGTTTTCTTGAAAATTG
TTAATTTCAAGACTGGAGAAATAAACTCACCTTCTATTTAAAAAAAAAAAAAAAAAAAA
>OriGene 5' read for SC322710 unedited
GTGGCATTTTAGTGCATTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCG
GTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGGCCCAGGACTGCAG
CGGCTTCGGAAGGTGGGCTCTGCCAGCGGGACCATGCTGCTCCGAGCCGCTTGGAGGCGG
GCGGCAGTGGCGGTGACAGCGGCTCCAGGGCCGAAGCCCGCGGCGCCCACTCGGGGGCTG
CGCCTGCGCGTTGGAGACCGTGCTCCTCAGTCTGCGGTTCCCGCAGATACAGCCGCTGCC
CCGGAGGTGGGGCCAGTGCTGCGACCTCTCTATATGGATGTGCAAGCTACAACTCCTCTG
GACCCCCGGGTGCTTGATGCCATGCTCCCTTACCTAATTAACTACTATGGGAACCCACAC
TCCCGGACACATGCTTATGGCTGGGAGAGTGAGGCAGCCATGGAACGTGCTCGTCAGCAA
GTAGCATCTCTGATTGGAGCTGATCCTCGTGAGATCATTTTTACTAGTGGTGCTACTGAA
TCCAACAACATAGCAATTAAGGGGGTGGCCCGATTCTACAGGTCACGGAAAAAGCACTTG
ATCACCACCCACACAGAACACAAATGTGTCTTGGACTCCTGCCGTTCACTGGAAAGCTGA
GGGCTTTCAGGTCACCTACCCTCCCAGTGCAAAAGAGTGGGATCATTGACCTAAAGGAAC
TAGAGGCTGCTATCCAGCCAGATACTAGCCTGGTGTCAGTCATGACTGTGAACAATGAGA
TTGGAGTGAAGCAGCCTATTGCAAAAATAGGCGGATTTGCAGTTTCCAGAAAGGTTATAT
TTCATACTGATGCAGCCAGGCTGTTGGAAAATCCACTGATGTCCATGACTGAAATGATCT
CATGAACATTATGGTCACAAAATCTACGGTCCAACGTGGTGCCATTACTCGTCCCGCCAA
TGTTGCCGTGTTGAGAGG
Restriction Sites Please inquire     
ACCN NM_021100
ORF Size 1374 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Reference Data
RefSeq NM_021100.3, NP_066923.3
RefSeq Size 2371
RefSeq ORF 1374
Locus ID 9054
Domains aminotran_5
Protein Pathways Thiamine metabolism
Gene Summary Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]
Transcript Variant: This variant (1) encodes the longer isoform (a).

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