NFS1 (NM_021100) Human Untagged Clone

CAT#: SC322710

NFS1 (untagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFS1
• Synonyms: HUSSY-08; IscS; NIFS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322710 GGGCCCAGGACTGCAGCGGCTTCGGAAGGTGGGCTCTGCCAGCGGGACCATGCTGCTCCG
  AGCCGCTTGGAGGCGGGCGGCAGTGGCGGTGACAGCGGCTCCAGGGCCGAAGCCCGCGGC
  GCCCACTCGGGGGCTGCGCCTGCGCGTTGGAGACCGTGCTCCTCAGTCTGCGGTTCCCGC
  AGATACAGCCGCTGCCCCGGAGGTGGGGCCAGTGCTGCGACCTCTCTATATGGATGTGCA
  AGCTACAACTCCTCTGGACCCCCGGGTGCTTGATGCCATGCTCCCTTACCTAATTAACTA
  CTATGGGAACCCACACTCCCGGACACATGCTTATGGCTGGGAGAGTGAGGCAGCCATGGA
  ACGTGCTCGTCAGCAAGTAGCATCTCTGATTGGAGCTGATCCTCGTGAGATCATTTTTAC
  TAGTGGTGCTACTGAATCCAACAACATAGCAATTAAGGGGGTGGCCCGATTCTACAGGTC
  ACGGAAAAAGCACTTGATCACCACCCAGACAGAACACAAATGTGTCTTGGACTCCTGCCG
  TTCACTGGAAGCTGAGGGCTTTCAGGTCACCTACCTCCCAGTGCAGAAGAGTGGGATCAT
  TGACCTAAAGGAACTAGAGGCTGCTATCCAGCCAGATACTAGCCTGGTGTCAGTCATGAC
  TGTGAACAATGAGATTGGAGTGAAGCAGCCTATTGCAGAAATAGGGCGGATTTGCAGTTC
  CAGAAAGGTATATTTCCATACTGATGCAGCCCAGGCTGTTGGAAAAATCCCACTTGATGT
  CAATGACATGAAAATTGATCTCATGAGCATTAGTGGTCACAAAATCTACGGTCCCAAAGG
  GGTTGGTGCCATCTACATCCGTCGCCGGCCCCGTGTGCGTGTGGAGGCCCTGCAGAGTGG
  AGGGGGGCAGGAGCGGGGTATGCGGTCTGGGACAGTGCCCACACCCTTAGTGGTGGGGCT
  GGGGGCTGCGTGTGAGGTGGCACAGCAAGAGATGGAGTATGACCACAAGCGAATCTCAAA
  GTTGTCAGAGCGGCTGATACAGAATATAATGAAGAGCCTTCCAGATGTGGTGATGAATGG
  GGACCCTAAGCACCATTATCCCGGCTGTATCAACCTCTCCTTTGCATATGTGGAAGGGGA
  AAGTCTGCTGATGGCACTGAAGGACGTTGCCTTATCCTCAGGGAGTGCCTGCACCTCTGC
  ATCCCTGGAGCCCTCTTATGTGCTTAGAGCAATTGGCACTGATGAGGATTTAGCGCACTC
  TTCTATCAGGTTTGGAATTGGCCGCTTCACTACAGAGGAGGAAGTGGACTACACAGTGGA
  GAAATGCATTCAGCATGTGAAGCGTCTTCGAGAAATGAGCCCTCTCTGGGAGATGGTTCA
  GGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAACACTAGAAGAATAGGGCCCTGAC
  TTTGTGCTGGTCTGGCCCCTCCTGCCTCACCAACCCGTGCACAACCAGACACCTTGTTAC
  ACCTAGTGGATGCTCTAGATTGGTATAGACCAGTTGACTTCAGCATCAGTCCACCTCTAT
  GACAGAAACACAAGAAAACTGTCTTTCCCTAGCTTCAGTTCCTTGGGTGTGGAGCACTCC
  CCATTTCTTCTCGGGTCTTAAAGTGTGTGGACATTTTCATCCCGAAGCCATAGAGACATT
  TGCTGTCATATTGCTGCTGGGCACATCTGTGCTCTTGGTGAGGAGAGCAAGAGGAACCAG
  AAGAAGTCTCTTTGGTCAGGGACCATGATGCTCTACATGGACATTTGAGTCTTCGTCTTC
  TGCTGCTGCTCGGCTGGACCAGCTTCTTTAACAGCAAGCATAATCCACTTCAATGTAATA
  TTTTCTGTAGCTCCAAAGGCTATCTCTTCATATTGACTGCAGACAGACTGAATGGACAGT
  TTCTTAGAGGGCTTGTCTCCTTTCTACCCTTGTCCTCTTTCCTTTCCTTTGACCTAATGG
  AGCTAGAAATATGTCTGTGACTCCACCAGTTATTCTAATAATTTGTTTTCTTGAAAATTG
  TTAATTTCAAGACTGGAGAAATAAACTCACCTTCTATTTAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for SC322710 unedited
  GTGGCATTTTAGTGCATTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCG
  GTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGGCCCAGGACTGCAG
  CGGCTTCGGAAGGTGGGCTCTGCCAGCGGGACCATGCTGCTCCGAGCCGCTTGGAGGCGG
  GCGGCAGTGGCGGTGACAGCGGCTCCAGGGCCGAAGCCCGCGGCGCCCACTCGGGGGCTG
  CGCCTGCGCGTTGGAGACCGTGCTCCTCAGTCTGCGGTTCCCGCAGATACAGCCGCTGCC
  CCGGAGGTGGGGCCAGTGCTGCGACCTCTCTATATGGATGTGCAAGCTACAACTCCTCTG
  GACCCCCGGGTGCTTGATGCCATGCTCCCTTACCTAATTAACTACTATGGGAACCCACAC
  TCCCGGACACATGCTTATGGCTGGGAGAGTGAGGCAGCCATGGAACGTGCTCGTCAGCAA
  GTAGCATCTCTGATTGGAGCTGATCCTCGTGAGATCATTTTTACTAGTGGTGCTACTGAA
  TCCAACAACATAGCAATTAAGGGGGTGGCCCGATTCTACAGGTCACGGAAAAAGCACTTG
  ATCACCACCCACACAGAACACAAATGTGTCTTGGACTCCTGCCGTTCACTGGAAAGCTGA
  GGGCTTTCAGGTCACCTACCCTCCCAGTGCAAAAGAGTGGGATCATTGACCTAAAGGAAC
  TAGAGGCTGCTATCCAGCCAGATACTAGCCTGGTGTCAGTCATGACTGTGAACAATGAGA
  TTGGAGTGAAGCAGCCTATTGCAAAAATAGGCGGATTTGCAGTTTCCAGAAAGGTTATAT
  TTCATACTGATGCAGCCAGGCTGTTGGAAAATCCACTGATGTCCATGACTGAAATGATCT
  CATGAACATTATGGTCACAAAATCTACGGTCCAACGTGGTGCCATTACTCGTCCCGCCAA
  TGTTGCCGTGTTGAGAGG
• Restriction Sites: Please inquire
• ACCN: NM_021100
• ORF Size: 1374 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_021100.3, NP_066923.3
• RefSeq Size: 2371
• RefSeq ORF: 1374
• Locus ID: 9054
• Domains: aminotran_5
• Protein Pathways: Thiamine metabolism
• Gene Summary: Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) encodes the longer isoform (a).