AKAP12 (NM_144497) Human Untagged Clone

CAT#: SC110079

AKAP12 (untagged)-Human A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant 2


  "NM_144497" in other vectors (2)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol AKAP12
Synonyms AKAP250; SSeCKS
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC110079 sequence for NM_144497 edited (data generated by NextGen Sequencing)
ATGCTGGGGACCATCACCATCACAGTTGGACAGAGAGACTCTGAAGATGTGAGCAAAAGA
GACTCCGATAAAGAGATGGCTACTAAGTCAGCGGTTGTTCACGACATCACAGATGATGGG
CAGGAGGAGACACCCGAAATAATCGAACAGATTCCTTCTTCAGAAAGCAATTTAGAAGAG
CTAACACAACCCACTGAGTCCCAGGCTAATGATATTGGATTTAAGAAGGTGTTTAAGTTT
GTTGGCTTTAAATTCACTGTGAAAAAGGATAAGACAGAGAAGCCTGACACTGTCCAGCTA
CTCACTGTGAAGAAAGATGAAGGGGAGGGAGCAGCAGGGGCTGGCGACCACAAGGACCCC
AGCCTTGGGGCTGGAGAAGCAGCATCCAAAGAAAGCGAACCCAAACAATCTACAGAGAAA
CCCGAAGAGACCCTGAAGCGTGAGCAAAGCCACGCAGAAATTTCTCCCCCAGCCGAATCT
GGCCAAGCAGTGGAGGAATGCAAAGAGGAAGGAGAAGAGAAACAAGAAAAAGAACCTAGC
AAGTCTGCAGAATCTCCGACTAGTCCCGTGACCAGTGAAACAGGATCAACCTTCAAAAAA
TTCTTCACTCAAGGTTGGGCCGGCTGGCGCAAAAAGACCAGTTTCAGGAAGCCGAAGGAG
GATGAAGTGGAAGCTTCAGAGAAGAAAAAGGAACAAGAGCCAGAAAAAGTAGACACAGAA
GAAGACGGAAAGGCAGAGGTTGCCTCCGAGAAACTGACCGCCTCCGAGCAAGCCCACCCA
CAGGAGCCGGCAGAAAGTGCCCACGAGCCCCGGTTATCAGCTGAATATGAGAAAGTTGAG
CTGCCCTCAGAGGAGCAAGTCAGTGGCTCGCAGGGACCTTCTGAAGAGAAACCTGCTCCG
TTGGCGACAGAAGTGTTTGATGAGAAAATAGAAGTCCACCAAGAAGAGGTTGTGGCCGAA
GTCCACGTCAGCACCGTGGAGGAGAGAACCGAAGAGCAGAAAACGGAGGTGGAAGAAACA
GCAGGGTCTGTGCCAGCTGAAGAATTGGTTGAAATGGATGCAGAACCTCAGGAAGCTGAA
CCTGCCAAGGAGCTGGTGAAGCTCAAAGAAACGTGTGTTTCCGGAGAGGACCCTACACAG
GGAGCTGACCTCAGTCCTGATGAGAAGGTGCTGTCCAAACCCCCCGAAGGCGTTGTGAGT
GAGGTGGAAATGCTGTCATCACAGGAGAGAATGAAGGTGCAGGGAAGTCCACTAAAGAAG
CTTTTTACCAGCACTGGCTTAAAAAAGCTTTCTGGAAAGAAACAGAAAGGGAAAAGAGGA
GGAGGAGACGAGGAATCAGGGGAGCACACTCAGGTTCCAGCCGATTCTCCGGACAGCCAG
GAGGAGCAAAAGGGCGAGAGCTCTGCCTCATCCCCTGAGGAGCCCGAGGAGATCACGTGT
CTGGAAAAGGGCTTAGCCGAGGTGCAGCAGGATGGGGAAGCTGAAGAAGGAGCTACTTCC
GATGGAGAGAAAAAAAGAGAAGGTGTCACTCCCTGGGCATCATTCAAAAAGATGGTGACG
CCCAAGAAGCGTGTTAGACGGCCTTCGGAAAGTGATAAAGAAGATGAGCTGGACAAGGTC
AAGAGCGCTACCTTGTCTTCCACCGAGAGCACAGCCTCTGAAATGCAAGAAGAAATGAAA
GGGAGCGTGGAAGAGCCAAAGCCGGAAGAACCAAAGCGCAAGGTGGATACCTCAGTATCT
TGGGAAGCTTTAATTTGTGTGGGATCATCCAAGAAAAGAGCAAGGAGAGGGTCCTCTTCT
GATGAGGAAGGGGGACCAAAAGCAATGGGAGGAGACCACCAGAAAGCTGATGAGGCCGGA
AAAGACAAAGAGACGGGGACAGACGGGATCCTTGCTGGTTCCCAAGAACATGATCCAGGG
CAGGGAAGTTCCTCCCCGGAGCAAGCTGGAAGCCCTACCGAAGGGGAGGGCGTTTCCACC
TGGGAGTCATTTAAAAGGTTAGTCACGCCAAGAAAAAAATCAAAGTCCAAGCTGGAAGAG
AAAAGCGAAGACTCCATAGCTGGGTCTGGTGTAGAACATTCCACTCCAGACACTGAACCC
GGTAAAGAAGAATCCTGGGTCTCAATCAAGAAGTTTATTCCTGGACGAAGGAAGAAAAGG
CCAGATGGGAAACAAGAACAAGCCCCTGTTGAAGACGCAGGGCCAACAGGGGCCAACGAA
GATGACTCTGATGTCCCGGCCGTGGTCCCTCTGTCTGAGTATGATGCTGTAGAAAGGGAG
AAAATGGAGGCACAGCAAGCCCAAAAAAGCGCAGAGCAGCCCGAGCAGAAGGCAGCCACT
GAGGTGTCCAAGGAGCTCAGCGAGAGTCAGGTTCATATGATGGCAGCAGCTGTCGCTGAC
GGGACGAGGGCAGCTACCATTATTGAAGAAAGGTCTCCTTCTTGGATATCTGCTTCAGTG
ACAGAACCTCTTGAACAAGTAGAAGCTGAAGCCGCACTGTTAACTGAGGAGGTATTGGAA
AGAGAAGTAATTGCAGAAGAAGAACCCCCCACGGTTACTGAACCTCTGCCAGAGAACAGA
GAGGCCCGGGGCGACACGGTCGTTAGTGAGGCGGAATTGACCCCCGAAGCTGTGACAGCT
GCAGAAACTGCAGGGCCATTGGGTGCCGAAGAAGGAACCGAAGCATCTGCTGCTGAAGAG
ACCACAGAAATGGTGTCAGCAGTCTCCCAGTTAACCGACTCCCCAGACACCACAGAGGAG
GCCACTCCGGTGCAGGAGGTGGAAGGTGGCGTACCTGACATAGAAGAGCAAGAGAGGCGG
ACTCAAGAGGTCCTCCAGGCAGTGGCAGAAAAAGTGAAAGAGGAATCCCAGCTGCCTGGC
ACCGGTGGGCCAGAAGATGTGCTTCAGCCTGTGCAGAGAGCAGAGGCAGAAAGACCAGAA
GAGCAGGCTGAAGCGTCGGGTCTGAAGAAAGAGACGGATGTAGTGTTGAAAGTAGATGCT
CAGGAGGCAAAAACTGAGCCTTTTACACAAGGGAAGGTGGTGGGGCAGACCACCCCAGAA
AGCTTTGAAAAAGCTCCTCAAGTCACAGAGAGCATAGAGTCCAGTGAGCTTGTAACCACT
TGTCAAGCCGAAACCTTAGCTGGGGTAAAATCACAGGAGATGGTGATGGAACAGGCTATC
CCCCCTGACTCGGTGGAAACCCCTACAGACAGTGAGACTGATGGAAGCACCCCCGTAGCC
GACTTTGACGCACCAGGCACAACCCAGAAAGACGAGATTGTGGAAATCCATGAGGAGAAT
GAGGTCGCATCTGGTACCCAGTCAGGGGGCACAGAAGCAGAGGCAGTTCCTGCACAGAAA
GAGAGGCCTCCAGCACCTTCCAGTTTTGTGTTCCAGGAAGAAACTAAAGAACAATCAAAG
ATGGAAGACACTCTAGAGCATACAGATAAAGAGGTGTCAGTGGAAACTGTATCCATTCTG
TCAAAGACTGAGGGGACTCAAGAGGCTGACCAGTATGCTGATGAGAAAACCAAAGACGTA
CCATTTTTCGAAGGACTTGAGGGGTCTATAGACACAGGCATAACAGTCAGTCGGGAAAAG
GTCACTGAAGTTGCCCTTAAAGGTGAAGGGACAGAAGAAGCTGAATGTAAAAAGGATGAT
GCTCTTGAACTGCAGAGTCACGCTAAGTCTCCTCCATCCCCCGTGGAGAGAGAGATGGTA
GTTCAAGTCGAAAGGGAGAAAACAGAAGCAGAGCCAACCCATGTGAATGAAGAGAAGCTT
GAGCACGAAACAGCTGTTACCGTATCTGAAGAGGTCAGTAAGCAGCTCCTCCAGACAGTG
AATGTGCCCATCATAGATGGGGCAAAGGAAGTCAGCAGTTTGGAAGGAAGCCCTCCTCCC
TGCCTAGGTCAAGAGGAGGCAGTATGCACCAAAATTCAAGTTCAGAGCTCTGAGGCATCA
TTCACTCTAACAGCGGCTGCAGAGGAGGAAAAGGTCTTAGGAGAAACTGCCAACATTTTA
GAAACAGGTGAAACGTTGGAGCCTGCAGGTGCACATTTAGTTCTGGAAGAGAAATCCTCT
GAAAAAAATGAAGACTTTGCCGCTCATCCAGGGGAAGATGCTGTGCCCACAGGGCCCGAC
TGTCAGGCAAAATCGACACCAGTGATAGTATCTGCTACTACCAAGAAAGGCTTAAGTTCG
GACCTGGAAGGAGAGAAAACCACATCACTGAAGTGGAAGTCAGATGAAGTCGATGAGCAG
GTTGCTTGCCAGGAGGTCAAAGTGAGTGTAGCAATTGAGGATTTAGAGCCTGAAAATGGG
ATTTTGGAACTTGAGACCAAAAGCAGTAAACTTGTCCAAAACATCATCCAGACAGCCGTT
GACCAGTTTGTACGTACAGAAGAAACAGCCACCGAAATGTTGACGTCTGAGTTACAGACA
CAAGCTCACGTGATAAAAGCTGACAGCCAGGACGCTGGACAGGAAACGGAGAAAGAAGGA
GAGGAACCTCAGGCCTCTGCACAGGATGAAACACCAATTACTTCAGCCAAAGAGGAGTCA
GAGTCAACCGCAGTGGGACAAGCACATTCTGATATTTCCAAAGACATGAGTGAAGCCTCA
GAAAAGACCATGACTGTTGAGGTAGAAGGTTCCACTGTAAATGATCAGCAGCTGGAAGAG
GTCGTCCTCCCATCTGAGGAAGAGGGAGGTGGAGCTGGAACAAAGTCTGTGCCAGAAGAT
GATGGTCATGCCTTGTTAGCAGAAAGAATAGAGAAGTCACTAGTTGAACCGAAAGAAGAT
GAAAAAGGTGATGATGTTGATGACCCTGAAAACCAGAACTCAGCCCTGGCTGATACTGAT
GCCTCAGGAGGCTTAACCAAAGAGTCCCCAGATACAAATGGACCAAAACAAAAAGAGAAG
GAGGATGCCCAGGAAGTAGAATTGCAGGAAGGAAAAGTGCACAGTGAATCAGATAAAGCG
ATCACACCCCAAGCACAGGAGGAGTTACAGAAACAAGAGAGAGAATCTGCAAAGTCAGAA
CTTACAGAATCTTAA

Clone variation with respect to NM_144497.2
4200 c=>g
>OriGene 5' read for NM_144497 unedited
GCGGCCGGCGAATTCGCACGAGGGTCTGGGCGCTCAGTCCGCTCTGATCCCGCCGAACCA
CCTGCGGTTGGCAGGCAGGAGACTAGGCGTCTGCCGGGGAGGGCAGGGACCCGCTAAGCT
GATCTCCTGTACAGTAGTGCTACTTAAAATATGCTGGGGACCATCACCATCACAGTTGGA
CAGAGAGACTCTGAAGATGTGAGCAAAAGAGACTCCGATAAAGAGATGGCTACTAAGTCA
GCGGTTGTTCACGACATCACAGATGATGGGCAGGAGGAGACACCCGAAATAATCGAACAG
ATTCCTTCTTCAGAAAGCAATTTAGAAGAGCTAACACAACCCACTGAGTCCCAGGCTAAT
GATATTGGATTTAAGAAGGTGTTTAAGTTTGTTGGCTTTAAATTCACTGTGAAAAAGGAT
AAGACAGAGAAGCCTGACACTGTCCAGCTACTCACTGTGAAGAAAGATGAAGGGGAGGGA
GCAGCAGGGGCTGGCGACCACAAGGACCCCAGCCTTGGGGCTGGAGAAGCAGCATCCAAA
GAAAGCGAACCCAAACAATCTACAGAGAAACCCGAAGAGACCCTGAAGCGTGAGCAAAGC
CACGCAGAAATTTCTCCCCCAGCCGAATCTGGCCAAGCAGTGGAGGAATGCAAAGAGGAA
GGAGAAGAGAAACAAGAANAAGAAACCTAGCAGTCTGCAGAATCTCCGACTAGTCCCGTG
ACCAGTGANACAGGATCAACCTTCANAAAATTCTTCACTCAAGGNTGGGCCNGNCTGGCG
CAAAAAGACCAGTTTCAGGAANCCCGAGGAGGATNNGAATGGAAGCTTCANAGAAGAAAA
GGGAACANGAGCCNGNANNAGTNNACACAGAAAAGACGGAAAGGCCANAGGTTA
>OriGene 3' read for NM_144497 unedited
TTTACGGAATTCTATGNNACCGCGGCCGCAATCTANNATCGAGTTTTTTTTTTTTTTTTT
TTTTTTTTTTTAAGTGAAGCAAATATTTTATTTAAATCAGTCGTCAAATCACAACTTATC
CAAATGAATGTGTTGCAACATTGTTCTTAAAAGAATGGCACAAATATGGCACAAACACAA
TCCAGTATCTATCAAAATACCATTTGTAAAGAACCTGACTTATTTCATGCCATGTGTGAA
TGCAGTCCAGCATATAAGAGTAGAATCAAAAACTTACAACAATTCTTTTCTTCATAACAT
ATAAAACATATTCACTACTTTGCCTCAGGATTAAGCACACTTATCGGTCCAAACAACCAT
AACAAGTAGGAAAAAGGATGTAAAGTTAATTTGCTACCAGAATATAACTTCTTTCTAACC
TCCACTGGGTTAGAAAGAAAAATTTCCCAATGACAATAGAACATTCTGTATGTTTCAAAA
TGCTTTTTAGTTTTAGCACACTCCGTGTAATAGGAAAGTGACCAATTTCAGAGTACCACT
AAATATGGACCTCAATCATTAGTGAATTTTAAAGAATTGTACCTTGATCAGGAATGTTAC
ATCTAGGAGGCTCTGTTTTTCGTGGGTTATATAACTGAAGCTTAAAGCTCCCCAAGCCTG
CACTATGGCACATGCCCCATCATATACTGTGTGATACAAAAAACATGTAGTATCCTGCAC
ATTTCCCTTAACATTAAGAAAAAAATATTCCATAGATACCGGAGGACTACTTTAAACATA
CATATAAATATGTAAAGGATGATACCTCCAGTTTCAGAAACAGAACTAGGTATTGCCCAA
CTCCATTTTCAGGTTAAAAATTTTAGGACCCTTAATCAGA
Restriction Sites NotI-NotI     
ACCN NM_144497
ORF Size 5055 bp
Insert Size 6000
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq NM_144497.1, NP_653080.1
RefSeq Size 6287
RefSeq ORF 5055
Locus ID 9590
Domains PkinA_anch
Protein Families Druggable Genome
Gene Summary The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) has an alternate 5' segment, compared to variant 1, that results in a distinct 5' UTR and 5' coding region. The resulting isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.

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