Chk2 (CHEK2) (NM_007194) Human Untagged Clone

CAT#: SC110240

CHEK2 (untagged)-Human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHEK2
• Synonyms: CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_007194 edited
  ATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAG
  CCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGC
  TCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTG
  AGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCT
  GAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAG
  GATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGC
  TGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGC
  AAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATA
  GAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGC
  CGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTC
  TTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATAC
  ATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGG
  AAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCA
  GCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTA
  AATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTT
  TTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAA
  GAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAA
  AACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAG
  GACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTC
  ATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGG
  ACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGC
  CTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATC
  ACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTG
  GACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCC
  TTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCT
  GAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAG
  CGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCT
  GCTGTGTTGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007194 unedited
  CATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGGCTGCGGAGAG
  TGTGCGGCTCCGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCA
  GCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGG
  CTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAG
  CCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGA
  ACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCC
  TGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAA
  TGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCT
  GAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGT
  GGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGT
  AAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGC
  ACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTC
  AGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAANACTCTTGGAAGTGGTGC
  CTGTGGAGAGGGTAAGCTGGCTNTCGAGAGGANAACATGTAGAAAGTAGCCATTAAGATC
  ATCAGCANAAGGAAAAGTTGCTATTGGNTTCAGCAGAGAGGCAGACCCAGCTCTTCATGG
  NTGAANCAGAATAGAAATTTTGAAAAGCTAANTCATCCTTGCATCATCNAGATTAAAACT
  TTTTTGATGCAGAAGATATTATAATGTTTGNGAATATGATGGAANGGGGNNAAAGCTGTT
  GACAAAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007194 unedited
  CGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCAGTTCCATCAGGTTTTTAA
  TTGTACATCAGTGACTGTGAAAAAGCATTATTCCCATAATTAAAATACAAACTATAAAAA
  AACAGACTCAAAGAAAAGAAAGATGACAGAGTGAAAGAAGGTACATTTCTTTCGTGTTCA
  AACCACGGAGTTCACAACACAGCAGCACACACAGCTGGGCGCTTTGTGGTCTCGGCACCC
  TCGGCTTCCCCTTCACGGGGCCGCTTTCGACTAGTAGAAGGCTGGGCTAGAACCTGGGGT
  AGAGCTGTGGATTCATTTTCCTCAGACAGAAGATCTTGAAACTTTCTCTTCATGTCTTCA
  TCCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCGTAAAACGTGCCTTTGGATCCACT
  ACCAACAACTTCTTGACAAGGTCCAGAGCTTTCTCTGAGACTTCTGCCCAGACTTCAGGA
  ATGAAGTTGTATTTTCCACTGGTGATCTGATCCTTCAGTGACACTTGAGTCCTATGCTCA
  GAGAAAGGTGGATACCCACTAAGGCAGATAAAAAGAATAACTCCTAAACTCCAGCAGTCC
  ACAGCACGGTTATACCCAGCAGTCCCAACAGAAACAAGAACTTCAGGCGCCAAGTAGGTG
  GGGGTTCCACATAAGGTTCTCATGAGAGAGGTCTCTCCCAAAATCTTGGAGTGCCCAAAA
  TCAGTAATCTTTATAAGACAGTCCTCTTCTTGAGATGACAGTAAAACATTCTCTGGCTTT
  AAGTCACGGGGTATAATACCGTTTTCATGAAGGTACTGCACAGCCCAAGAGCATCTGGGT
  AAAAATAGAGCTTGCAGGTAGCTTCTTTTCAGGCGTTTATCCCCCACCACTTTGTCAAAC
  AGTTTCCCCCTTCATCATTNCAAACATATAATATTCTCTGCTCAAAAAGTTTTATCTTGA
  GAGCCAGGAGATTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007194
• ORF Size: 1632 bp
• Insert Size: 1920
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_007194.3, NP_009125.1
• RefSeq Size: 1862
• RefSeq ORF: 1632
• Locus ID: 11200
• Domains: FHA, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, p53 signaling pathway
• Gene Summary: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform a.