PUF60 (NM_078480) Human Untagged Clone

CAT#: SC110270

PUF60 (untagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1


  "NM_078480" in other vectors (4)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PUF60
Synonyms FIR; RoBPI; SIAHBP1; VRJS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_078480, the custom clone sequence may differ by one or more nucleotides


ATGGCGACGGCGACCATAGCTCTCCAGGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGGCGGCGGCGG
CGGCAGTGGTGGCAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGACTCCATCAAGATGGAGAACGG
GCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAGGCCCTTCAGAAGGCC
AAGAAGTACGCCATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAGCAGACCATCGCGCACCAGCAGCAGC
AGCTCACCAACCTGCAGATGGCAGCAGTGACAATGGGCTTTGGAGATCCTCTCTCACCTTTGCAATCGAT
GGCGGCTCAGCGGCAGCGGGCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTG
GGGGAGGACACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGTCCTGGGACT
CCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCGAAGCTGCACAGCTGGCCTT
GGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCAAGGTGGGCAGACCCAGCAACATAGGGCAG
GCCCAGCCCATCATAGACCAGTTGGCTGAGGAGGCACGGGCCTTCAACCGCATCTACGTGGCCTCTGTGC
ACCAGGACCTCTCAGACGATGACATCAAGAGCGTGTTTGAGGCCTTTGGCAAGATCAAGTCCTGCACACT
GGCCCGGGACCCCACAACTGGCAAGCACAAGGGCTACGGCTTCATTGAGTACGAGAAGGCCCAGTCGTCC
CAAGATGCTGTGTCTTCCATGAACCTCTTTGACCTGGGTGGCCAGTACTTGCGGGTGGGCAAGGCTGTCA
CACCGCCCATGCCCCTACTCACACCAGCCACGCCTGGAGGCCTCCCACCTGCCGCTGCTGTGGCAGCTGC
TGCAGCCACTGCCAAGATCACAGCTCAGGAAGCAGTGGCCGGAGCAGCGGTGCTGGGTACCCTGGGCACA
CCTGGACTGGTGTCCCCAGCACTGACCCTGGCCCAGCCCCTGGGCACTTTGCCCCAGGCTGTCATGGCTG
CCCAGGCACCTGGAGTCATCACAGGTGTGACCCCAGCCCGTCCTCCTATCCCGGTCACCATCCCCTCGGT
GGGAGTGGTGAACCCCATCCTGGCCAGCCCTCCAACGCTGGGTCTCCTGGAGCCCAAGAAGGAGAAGGAA
GAAGAGGAGCTGTTTCCCGAGTCAGAGCGGCCAGAGATGCTGAGCGAGCAGGAGCACATGAGCATCTCGG
GCAGTAGCGCCCGACACATGGTGATGCAGAAGCTGCTCCGCAAGCAGGAGTCTACAGTGATGGTTCTGCG
CAACATGGTGGACCCCAAGGACATCGATGATGACCTGGAAGGGGAGGTGACAGAGGAGTGTGGCAAGTTC
GGGGCCGTGAACCGCGTCATCATCTACCAAGAGAAACAAGGCGAGGAGGAGGATGCAGAAATCATTGTCA
AGATCTTTGTGGAGTTTTCCATAGCCTCTGAGACTCATAAGGCCATCCAGGCCCTCAATGGCCGCTGGTT
TGCTGGCCGCAAGGTGGTGGCTGAAGTGTACGACCAGGAGCGTTTTGATAACAGTGACCTCTCTGCGTGA


>OriGene 5' read for NM_078480 unedited
TATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGACGGACGCA
AGATGGCGACGGCGACCATAGCTCTCGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGG
CGGCGGCGGCGGCAGTGGTGGCAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGACT
CCATCAAGATGGAGAACGGGCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGC
CCGAGCAGCAGGAGGCCCTTCAGAAGGCCAAGAAGTACGCCATGGAGCAGAGCATCAAGA
GTGTGCTGGTGAAGCAGACCATCGCGCACCAGCAGCAGCAGCTCACCAACCTGCAGATGG
CAGCAGTGACAATGGGCTTTGGAGATCCTCTCTCACCTTTGCAATCGATGGCGGCTCAGC
GGCAGCGGGCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTGG
GGGAGGACACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGT
CCTGGGACTCCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCG
AAGCTGCACAGCTGGCCTTGGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCA
AGGTGGGCAGACCCAGCAACATANGGCANGCCCAGCCCATCATAGACCAGTTGGCTGAGG
AGGCACGGGCCTTCAACCGCATCTACGTGGCCTCTGTGCCCAGGACCTCTCAGACGATGA
CATCAAGAGCGTGTTTGTAGCCTTTGGCAAGACAAGTTCTGACACTGGCCCGGGACCCAC
ACTGGCAGCACAGGGCTACGCTTCATTGATACGAGAAGCCAGTCGTCCCAGAGCTTGGTC
TCCATGACCTCTTGACTGGTGCCAGTACTGCGGTGGGCAGGTGTACACCCCCATGCCT
>OriGene 3' read for NM_078480 unedited
TTGGACGCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTGCCAGCAGCATCCG
CACCTTTATCCGCACTGTAGGCTGGGCTGGGCAGAGCGCGCCTGGCCCCGGGGACACCAC
TGTATCACTATAAAACCCAGAGGAAACAAGGAACAAGTGCAAGTCCGGGGAGAGGGACCA
CTGTCACGCAGAGAGGTCACTGTTATCAAAACGCTCCTGGTCGTACACTTCAGCCACCAC
CTTGCGGCCAGCAAACCAGCGGCCATTGAGGGCCTGGATGGCCTTATGAGTCTCAGAGGC
TATGGAAAACTCCACAAAGATCTTGACAATGATTTCTGCATCCTCCTCCTCGCCTTGTTT
CTCTTGGTAGATGATGACGCGGTTCACGGCCCCGAACTTGCCACACTCCTCTGTCACCTC
CCCTTCCAGGTCATCATCGATGTCCTTGGGGTCCACCATGTTGCGCAGAACCATCACTGT
AGACTCCTGCTTGCGGAGCAGCTTCTGCATCACCATGTGTCGGGCGCTACTGCCCGAGAT
GCTCATGTGCTCCTGCTCGCTCAGCATCTCTGGCCGCTCTGACTCGGGAAACAGCTCCTC
TTCTTCCTTCTCCTTCTTGGGCTCCAGGAGACCCAGCGTTGGAGGGCTGGCCAGGATGGG
GTTCACCACTCCCACCGAGGGGATGGTGACCGGGATAGGAGGACGGGCTGGGGTCACACC
TGTGATGACTCCANGTGCCTGGGCAGCCATGACAGCCTGGGGCANAGTGCCCCAGGGCTG
GGCCAGGGTCAGTGCTGGGGACACCAGTCCAAGTGGCCCAGGGTACCCAGCACCGCTGCT
TCGGCCACTGCTTTCTGAGCTGTGATCTTGGCAATGGCTGCANCANCTGCCACAACAACG
GCAGGTGGGAAGGCCCTCCAGCGTGCTGGTGTGAATAAGGGCATGGNGCGGGTGAANCCT
TTGCCAACCGCAGTACTGGGCCACCAGTTCAAAAAGTTCATGGAAAAACCACATCTTGGG
ACAACGA
Restriction Sites NotI-NotI     
ACCN NM_078480
ORF Size 1680 bp
Insert Size 1860
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq NM_078480.1, NP_510965.1
RefSeq Size 1946
RefSeq ORF 1680
Locus ID 22827
Domains RRM, RRM_1
Protein Pathways Spliceosome
Gene Summary This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
Transcript Variant: This variant (1) encodes the isoform a.

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