Dicer (DICER1) (NM_030621) Human Untagged Clone

CAT#: SC110307

DICER1 (untagged)-Human dicer 1, ribonuclease type III (DICER1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DICER1
• Synonyms: DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_030621 edited
  GAGAGCAGAAGCTTGGGCATGCTGTGATTTTCCAATAAACTGCTATCACAATGTCAAAAT
  GCAGTTCAGACAAGAGCAACACAGAGATCTCAAACATTAAAACGTAAGCTGTGCTAGAAC
  AAAAATGCAATGAAAGAAACACTGGATGAATGAAAAGCCCTGCTTTGCAACCCCTCAGCA
  TGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTGGACTGC
  CATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATATCAGGTTGAAC
  TGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAGGGAAGA
  CATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACTTCAGCA
  GAAATGGAAAAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGT
  CAGCTGTCAGAACTCATTCAGATCTCAAGGTTGGGGAATACTCAAACCTAGAAGTAAATG
  CATCTTGGACAAAAGAGAGATGGAACCAAGAGTTTACTAAGCACCAGGTTCTCATTATGA
  CTTGCTATGTCGCCTTGAATGTTTTGAAAAATGGTTACTTATCACTGTCAGACATTAACC
  TTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCACCCCTATCGAGAAATTATGA
  AGCTCTGTGAAAATTGTCCATCATGTCCTCGCATTTTGGGACTAACTGCTTCCATTTTAA
  ATGGGAAATGTGATCCAGAGGAATTGGAAGAAAAGATTCAGAAACTAGAGAAAATTCTTA
  AGAGTAATGCTGAAACTGCAACTGACCTGGTGGTCTTAGACAGGTATACTTCTCAGCCAT
  GTGAGATTGTGGTGGATTGTGGACCATTTACTGACAGAAGTGGGCTTTATGAAAGACTGC
  TGATGGAATTAGAAGAAGCACTTAATTTTATCAATGATTGTAATATATCTGTACATTCAA
  AAGAAAGAGATTCTACTTTAATTTCGAAACAGATACTATCAGACTGTCGTGCCGTATTGG
  TAGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCTGGAATGATGGTAAGAGAACTACAGA
  AATACATCAAACATGAGCAAGAGGAGCTGCACAGGAAATTTTTATTGTTTACAGACACTT
  TCCTAAGGAAAATACATGCACTATGTGAAGAGCACTTCTCACCTGCCTCACTTGACCTGA
  AATTTGTAACTCCTAAAGTAATCAAACTGCTCGAAATCTTACGCAAATATAAACCATATG
  AGCGACAGCAGTTTGAAAGCGTTGAGTGGTATAATAATAGAAATCAGGATAATTATGTGT
  CATGGAGTGATTCTGAGGATGATGATGAGGATGAAGAAATTGAAGAAAAAGAGAAGCCAG
  AGACAAATTTTCCTTCTCCTTTTACCAACATTTTGTGCGGAATTATTTTTGTGGAAAGAA
  GATACACAGCAGTTGTCTTAAACAGATTGATAAAGGAAGCTGGCAAACAAGATCCAGAGC
  TGGCTTATATCAGTAGCAATTTCATAACTGGACATGGCATTGGGAAGAATCAGCCTCGCA
  ACAAACAGATGGAAGCAGAATTCAGAAAACAGGAAGAGGTACTTAGGAAATTTCGAGCAC
  ATGAGACCAACCTGCTTATTGCAACAAGTATTGTAGAAGAGGGTGTTGATATACCAAAAT
  GCAACTTGGTGGTTCGTTTTGATTTGCCCACAGAATATCGATCCTATGTTCAATCTAAAG
  GAAGAGCAAGGGCACCCATCTCTAATTATATAATGTTAGCGGATACAGACAAAATAAAAA
  GTTTTGAAGAAGACCTTAAAACCTACAAAGCTATTGAAAAGATCTTGAGAAACAAGTGTT
  CCAAGTCGGTTGATACTGGTGAGACTGACATTGATCCTGTCATGGATGATGATGACGTTT
  TCCCACCATATGTGTTGAGGCCTGACGATGGTGGTCCACGAGTCACAATCAACACGGCCA
  TTGGACACATCAATAGATACTGTGCTAGATTACCAAGTGATCCGTTTACTCATCTAGCTC
  CTAAATGCAGAACCCGAGAGTTGCCTGATGGTACATTTTATTCAACTCTTTATCTGCCAA
  TTAACTCACCTCTTCGAGCCTCCATTGTTGGTCCACCAATGAGCTGTGTACGATTGGCTG
  AAAGAGTTGTAGCTCTCATTTGCTGTGAGAAACTGCACAAAATTGGCGAACTGGATGACC
  ATTTGATGCCAGTTGGGAAAGAGACTGTTAAATATGAAGAGGAGCTTGATTTGCATGATG
  AAGAAGAGACCAGTGTTCCAGGAAGACCAGGTTCCACGAAACGAAGGCAGTGCTACCCAA
  AAGCAATTCCAGAGTGTTTGAGGGATAGTTATCCCAGACCTGATCAGCCCTGTTACCTGT
  ATGTGATAGGAATGGTTTTAACTACACCTTTACCTGATGAACTCAACTTTAGAAGGCGGA
  AGCTCTATCCTCCTGAAGATACCACAAGATGCTTTGGAATACTGACGGCCAAACCCATAC
  CTCAGATTCCACACTTTCCTGTGTACACACGCTCTGGAGAGGTTACCATATCCATTGAGT
  TGAAGAAGTCTGGTTTCATGTTGTCTCTACAAATGCTTGAGTTGATTACAAGACTTCACC
  AGTATATATTCTCACATATTCTTCGGCTTGAAAAACCTGCACTAGAATTTAAACCTACAG
  ACGCTGATTCAGCATACTGTGTTCTACCTCTTAATGTTGTTAATGACTCCAGCACTTTGG
  ATATTGACTTTAAATTCATGGAAGATATTGAGAAGTCTGAAGCTCGCATAGGCATTCCCA
  GTACAAAGTATACAAAAGAAACACCCTTTGTTTTTAAATTAGAAGATTACCAAGATGCCG
  TTATCATTCCAAGATATCGCAATTTTGATCAGCCTCATCGATTTTATGTAGCTGATGTGT
  ACACTGATCTTACCCCACTCAGTAAATTTCCTTCCCCTGAGTATGAAACTTTTGCAGAAT
  ATTATAAAACAAAGTACAACCTTGACCTAACCAATCTCAACCAGCCACTGCTGGATGTGG
  ACCACACATCTTCAAGACTTAATCTTTTGACACCTCGACATTTGAATCAGAAGGGGAAAG
  CGCTTCCTTTAAGCAGTGCTGAGAAGAGGAAAGCCAAATGGGAAAGTCTGCAGAATAAAC
  AGATACTGGTTCCAGAACTCTGTGCTATACATCCAATTCCAGCATCACTGTGGAGAAAAG
  CTGTTTGTCTCCCCAGCATACTTTATCGCCTTCACTGCCTTTTGACTGCAGAGGAGCTAA
  GAGCCCAGACTGCCAGCGATGCTGGCGTGGGAGTCAGATCACTTCCTGCGGATTTTAGAT
  ACCCTAACTTAGACTTCGGGTGGAAAAAATCTATTGACAGCAAATCTTTCATCTCAATTT
  CTAACTCCTCTTCAGCTGAAAATGATAATTACTGTAAGCACAGCACAATTGTCCCTGAAA
  ATGCTGCACATCAAGGTGCTAATAGAACCTCCTCTCTAGAAAATCATGACCAAATGTCTG
  TGAACTGCAGAACGTTGCTCAGCGAGTCCCCTGGTAAGCTCCACGTTGAAGTTTCAGCAG
  ATCTTACAGCAATTAATGGTCTTTCTTACAATCAAAATCTCGCCAATGGCAGTTATGATT
  TAGCTAACAGAGACTTTTGCCAAGGAAATCAGCTAAATTACTACAAGCAGGAAATACCCG
  TGCAACCAACTACCTCATATTCCATTCAGAATTTATACAGTTACGAGAACCAGCCCCAGC
  CCAGCGATGAATGTACTCTCCTGAGTAATAAATACCTTGATGGAAATGCTAACAAATCTA
  CCTCAGATGGAAGTCCTGTGATGGCCGTAATGCCTGGTACGACAGACACTATTCAAGTGC
  TCAAGGGCAGGATGGATTCTGAGCAGAGCCCTTCTATTGGGTACTCCTCAAGGACTCTTG
  GCCCCAATCCTGGACTTATTCTTCAGGCTTTGACTCTGTCAAACGCTAGTGATGGATTTA
  ACCTGGAGCGGCTTGAAATGCTTGGCGACTCCTTTTTAAAGCATGCCATCACCACATATC
  TATTTTGCACTTACCCTGATGCGCATGAGGGCCGCCTTTCATATATGAGAAGCAAAAAGG
  TCAGCAACTGTAATCTGTATCGCCTTGGAAAAAAGAAGGGACTACCCAGCCGCATGGTGG
  TGTCAATATTTGATCCCCCTGTGAATTGGCTTCCTCCTGGTTATGTAGTAAATCAAGACA
  AAAGCAACACAGATAAATGGGAAAAAGATGAAATGACAAAAGACTGCATGCTGGCGAATG
  GCAAACTGGATGAGGATTACGAGGAGGAGGATGAGGAGGAGGAGAGCCTGATGTGGAGGG
  CTCCGAAGGAAGAGGCTGACTATGAAGATGATTTCCTGGAGTATGATCAGGAACATATCA
  GATTTATAGATAATATGTTAATGGGGTCAGGAGCTTTTGTAAAGAAAATCTCTCTTTCTC
  CTTTTTCAACCACTGATTCTGCATATGAATGGAAAATGCCCAAAAAATCCTCCTTAGGTA
  GTATGCCATTTTCATCAGATTTTGAGGATTTTGACTACAGCTCTTGGGATGCAATGTGCT
  ATCTGGATCCTAGCAAAGCTGTTGAAGAAGATGACTTTGTGGTGGGGTTCTGGAATCCAT
  CAGAAGAAAACTGTGGTGTTGACACGGGAAAGCAGTCCATTTCTTACGACTTGCACACTG
  AGCAGTGTATTGCTGACAAAAGCATAGCGGACTGTGTGGAAGCCCTGCTGGGCTGCTATT
  TAACCAGCTGTGGGGAGAGGGCTGCTCAGCTTTTCCTCTGTTCACTGGGGCTGAAGGTGC
  TCCCGGTAATTAAAAGGACTGATCGGGAAAAGGCCCTGTGCCCTACTCGGGAGAATTTCA
  ACAGCCAACAAAAGAACCTTTCAGTGAGCTGTGCTGCTGCTTCTGTGGCCAGTTCACGCT
  CTTCTGTATTGAAAGACTCGGAATATGGTTGTTTGAAGATTCCACCAAGATGTATGTTTG
  ATCATCCAGATGCAGATAAAACACTGAATCACCTTATATCGGGGTTTGAAAATTTTGAAA
  AGAAAATCAACTACAGATTCAAGAATAAGGCTTACCTTCTCCAGGCTTTTACACATGCCT
  CCTACCACTACAATACTATCACTGATTGTTACCAGCGCTTAGAATTCCTGGGAGATGCGA
  TTTTGGACTACCTCATAACCAAGCACCTTTATGAAGACCCGCGGCAGCACTCCCCGGGGG
  TCCTGACAGACCTGCGGTCTGCCCTGGTCAACAACACCATCTTTGCATCGCTGGCTGTAA
  AGTACGACTACCACAAGTACTTCAAAGCTGTCTCTCCTGAGCTCTTCCATGTCATTGATG
  ACTTTGTGCAGTTTCAGCTTGAGAAGAATGAAATGCAAGGAATGGATTCTGAGCTTAGGA
  GATCTGAGGAGGATGAAGAGAAAGAAGAGGATATTGAAGTTCCAAAGGCCATGGGGGATA
  TTTTTGAGTCGCTTGCTGGTGCCATTTACATGGATAGTGGGATGTCACTGGAGACAGTCT
  GGCAGGTGTACTATCCCATGATGCGGCCACTAATAGAAAAGTTTTCTGCAAATGTACCCC
  GTTCCCCTGTGCGAGAATTGCTTGAAATGGAACCAGAAACTGCCAAATTTAGCCCGGCTG
  AGAGAACTTACGACGGGAAGGTCAGAGTCACTGTGGAAGTAGTAGGAAAGGGGAAATTTA
  AAGGTGTTGGTCGAAGTTACAGGATTGCCAAATCTGCAGCAGCAAGAAGAGCCCTCCGAA
  GCCTCAAAGCTAATCAACCTCAGGTTCCCAATAGCTGAAACCGCTTTTTAAAATTCAAAA
  CA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030621 unedited
  GCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNATTCGGCACGAGCCGTGCTG
  CAGGTTACCTAGGGTATGAATTAATACAGACTTGGAAACTCTGAAAGAACTTAGAATCAG
  CATTTTGAGAGCAGAAGCTTGGGCATGCTGTGATTTTCCAATAAACTGCTATCACAATGT
  CAAAATGCAGTTCAGACAAGAGCAACACAGAGATCTCAAACATTAAAACGTAAGCTGTGC
  TAGAACAAAAATGCAATGAAAGAAACACTGGATGAATGAAAAGCCCTGCTTTGCAACCCC
  TCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTG
  GACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATATCAGG
  TTGAACTGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAG
  GGAAGACATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACT
  TCAGCAGAAATGGAAAAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAAC
  AAGTGTCAGCTGTCAGAACTCATTCAGATCTCAAGGTTGGGGAATACTCAAACCTAGAAG
  TAAATGCATCTTGGACAAAAGAGAGATGGAACCAAGAGTTTACTAAGCACCAGGTTCTCA
  TTATGACTTGCTATGTCGCCTTGAATGTTTTGAAAAATGGTTACTTATCACTGTCAGACA
  TTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCACCCCTATCGAGAAA
  TTATGAAGCTCTGTGAAAATGTCCATCATGTCCTCGCATTTTGGGACTAAACTGCTTCAT
  TNTAATGGGAAATGTGATCCAGAGGAATTGGNAGANAGATCAGAACTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030621 unedited
  TCTGTCACGCGGCCGCATCTATGATCGGTTTTTTTCTCTTTTTTTTTCCTTTCCAAGAGG
  AAATATGAGCTAGTTTATCTAACTCAATGACCTAAATCACAGATGTATCAAAATTACGGC
  AGTTTATCGCAAACGTTAAACTTTCACGGCATTAACAGCACAGCTTTGGTAGGTGAAATA
  TGCATGCAACATTACTGAAAAGTATAGCAAGAAATTTATGTGAGGAGACTAAGGGTAAAG
  GTGCTGTGTTTTGCTTCTTAAATAAGTAACCAATCAATTATAAAATCTGCAGCATATTTT
  AGGTGTGATATGCCTAAGGTTTATTTTGTTAGAGCAACAGCCTATAAGGCAAAATTATTT
  GCCATAAACATTGCCATCAGTGGGAAACTACCTGGCAGGAGGACTTTAGTAAAATGGGGC
  GCTTAGCCAAAGATCATTTTTATGATAAAAAATATCCGCAGACTACATATTGCTGGGTTT
  TAAAATGCCTTCAGCATACATATGACAGAAATTTTAGCCATGATCCCTGCAATAAGCTCA
  CACTATACTAGTGAGTCGCAAGCATCACGCTGCCTCAACGCGCAATGTATAACACCACGA
  AAGGATTGCATCACCAGCTCGCCCCAACAACATCACAGCTCAAAGACTATTCATGAAGGC
  CCCACTTTTAAGGCCAGCACTCCTTCTTATGCCAGACTATGCGGTCTCCACACACCTACA
  ATGCGATTCCCTCACCATGTTCGCTCATCGCCCACTCACACCCCTGTCCCTTTACTATTA
  GTAGTCGCCGCTCCCGCCCACACTCTTCTCCACCCGCTTCCCCGCCCGAAGCAAACCCTC
  TCCCGCCCGCCTCTCCCCCCGCGACCTCTCCCGCACCTATCGGCCCACAACTTCGCGGCT
  CCGCACCCTCCGCCACCCCGCCGTCAGCGGCACACTNTACCCCCTNCTCGTCCACCGCTT
  GTGCTCCGCTCTCCCCCTACTCACACCCACCTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030621
• ORF Size: 5769 bp
• Insert Size: 7500
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_030621.2, NP_085124.2
• RefSeq Size: 10220
• RefSeq ORF: 5769
• Locus ID: 23405
• Domains: RIBOc, DSRM, DEAD, helicase_C, PAZ, DUF283
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (2) represents the longest transcript. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.