Dicer (DICER1) (NM_030621) Human Untagged Clone

CAT#: SC110307

DICER1 (untagged)-Human dicer 1, ribonuclease type III (DICER1), transcript variant 2


  "NM_030621" in other vectors (3)

Reconstitution Protocol

USD 2,820.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DICER1
Synonyms DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_030621 edited
GAGAGCAGAAGCTTGGGCATGCTGTGATTTTCCAATAAACTGCTATCACAATGTCAAAAT
GCAGTTCAGACAAGAGCAACACAGAGATCTCAAACATTAAAACGTAAGCTGTGCTAGAAC
AAAAATGCAATGAAAGAAACACTGGATGAATGAAAAGCCCTGCTTTGCAACCCCTCAGCA
TGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTGGACTGC
CATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATATCAGGTTGAAC
TGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAGGGAAGA
CATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACTTCAGCA
GAAATGGAAAAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGT
CAGCTGTCAGAACTCATTCAGATCTCAAGGTTGGGGAATACTCAAACCTAGAAGTAAATG
CATCTTGGACAAAAGAGAGATGGAACCAAGAGTTTACTAAGCACCAGGTTCTCATTATGA
CTTGCTATGTCGCCTTGAATGTTTTGAAAAATGGTTACTTATCACTGTCAGACATTAACC
TTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCACCCCTATCGAGAAATTATGA
AGCTCTGTGAAAATTGTCCATCATGTCCTCGCATTTTGGGACTAACTGCTTCCATTTTAA
ATGGGAAATGTGATCCAGAGGAATTGGAAGAAAAGATTCAGAAACTAGAGAAAATTCTTA
AGAGTAATGCTGAAACTGCAACTGACCTGGTGGTCTTAGACAGGTATACTTCTCAGCCAT
GTGAGATTGTGGTGGATTGTGGACCATTTACTGACAGAAGTGGGCTTTATGAAAGACTGC
TGATGGAATTAGAAGAAGCACTTAATTTTATCAATGATTGTAATATATCTGTACATTCAA
AAGAAAGAGATTCTACTTTAATTTCGAAACAGATACTATCAGACTGTCGTGCCGTATTGG
TAGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCTGGAATGATGGTAAGAGAACTACAGA
AATACATCAAACATGAGCAAGAGGAGCTGCACAGGAAATTTTTATTGTTTACAGACACTT
TCCTAAGGAAAATACATGCACTATGTGAAGAGCACTTCTCACCTGCCTCACTTGACCTGA
AATTTGTAACTCCTAAAGTAATCAAACTGCTCGAAATCTTACGCAAATATAAACCATATG
AGCGACAGCAGTTTGAAAGCGTTGAGTGGTATAATAATAGAAATCAGGATAATTATGTGT
CATGGAGTGATTCTGAGGATGATGATGAGGATGAAGAAATTGAAGAAAAAGAGAAGCCAG
AGACAAATTTTCCTTCTCCTTTTACCAACATTTTGTGCGGAATTATTTTTGTGGAAAGAA
GATACACAGCAGTTGTCTTAAACAGATTGATAAAGGAAGCTGGCAAACAAGATCCAGAGC
TGGCTTATATCAGTAGCAATTTCATAACTGGACATGGCATTGGGAAGAATCAGCCTCGCA
ACAAACAGATGGAAGCAGAATTCAGAAAACAGGAAGAGGTACTTAGGAAATTTCGAGCAC
ATGAGACCAACCTGCTTATTGCAACAAGTATTGTAGAAGAGGGTGTTGATATACCAAAAT
GCAACTTGGTGGTTCGTTTTGATTTGCCCACAGAATATCGATCCTATGTTCAATCTAAAG
GAAGAGCAAGGGCACCCATCTCTAATTATATAATGTTAGCGGATACAGACAAAATAAAAA
GTTTTGAAGAAGACCTTAAAACCTACAAAGCTATTGAAAAGATCTTGAGAAACAAGTGTT
CCAAGTCGGTTGATACTGGTGAGACTGACATTGATCCTGTCATGGATGATGATGACGTTT
TCCCACCATATGTGTTGAGGCCTGACGATGGTGGTCCACGAGTCACAATCAACACGGCCA
TTGGACACATCAATAGATACTGTGCTAGATTACCAAGTGATCCGTTTACTCATCTAGCTC
CTAAATGCAGAACCCGAGAGTTGCCTGATGGTACATTTTATTCAACTCTTTATCTGCCAA
TTAACTCACCTCTTCGAGCCTCCATTGTTGGTCCACCAATGAGCTGTGTACGATTGGCTG
AAAGAGTTGTAGCTCTCATTTGCTGTGAGAAACTGCACAAAATTGGCGAACTGGATGACC
ATTTGATGCCAGTTGGGAAAGAGACTGTTAAATATGAAGAGGAGCTTGATTTGCATGATG
AAGAAGAGACCAGTGTTCCAGGAAGACCAGGTTCCACGAAACGAAGGCAGTGCTACCCAA
AAGCAATTCCAGAGTGTTTGAGGGATAGTTATCCCAGACCTGATCAGCCCTGTTACCTGT
ATGTGATAGGAATGGTTTTAACTACACCTTTACCTGATGAACTCAACTTTAGAAGGCGGA
AGCTCTATCCTCCTGAAGATACCACAAGATGCTTTGGAATACTGACGGCCAAACCCATAC
CTCAGATTCCACACTTTCCTGTGTACACACGCTCTGGAGAGGTTACCATATCCATTGAGT
TGAAGAAGTCTGGTTTCATGTTGTCTCTACAAATGCTTGAGTTGATTACAAGACTTCACC
AGTATATATTCTCACATATTCTTCGGCTTGAAAAACCTGCACTAGAATTTAAACCTACAG
ACGCTGATTCAGCATACTGTGTTCTACCTCTTAATGTTGTTAATGACTCCAGCACTTTGG
ATATTGACTTTAAATTCATGGAAGATATTGAGAAGTCTGAAGCTCGCATAGGCATTCCCA
GTACAAAGTATACAAAAGAAACACCCTTTGTTTTTAAATTAGAAGATTACCAAGATGCCG
TTATCATTCCAAGATATCGCAATTTTGATCAGCCTCATCGATTTTATGTAGCTGATGTGT
ACACTGATCTTACCCCACTCAGTAAATTTCCTTCCCCTGAGTATGAAACTTTTGCAGAAT
ATTATAAAACAAAGTACAACCTTGACCTAACCAATCTCAACCAGCCACTGCTGGATGTGG
ACCACACATCTTCAAGACTTAATCTTTTGACACCTCGACATTTGAATCAGAAGGGGAAAG
CGCTTCCTTTAAGCAGTGCTGAGAAGAGGAAAGCCAAATGGGAAAGTCTGCAGAATAAAC
AGATACTGGTTCCAGAACTCTGTGCTATACATCCAATTCCAGCATCACTGTGGAGAAAAG
CTGTTTGTCTCCCCAGCATACTTTATCGCCTTCACTGCCTTTTGACTGCAGAGGAGCTAA
GAGCCCAGACTGCCAGCGATGCTGGCGTGGGAGTCAGATCACTTCCTGCGGATTTTAGAT
ACCCTAACTTAGACTTCGGGTGGAAAAAATCTATTGACAGCAAATCTTTCATCTCAATTT
CTAACTCCTCTTCAGCTGAAAATGATAATTACTGTAAGCACAGCACAATTGTCCCTGAAA
ATGCTGCACATCAAGGTGCTAATAGAACCTCCTCTCTAGAAAATCATGACCAAATGTCTG
TGAACTGCAGAACGTTGCTCAGCGAGTCCCCTGGTAAGCTCCACGTTGAAGTTTCAGCAG
ATCTTACAGCAATTAATGGTCTTTCTTACAATCAAAATCTCGCCAATGGCAGTTATGATT
TAGCTAACAGAGACTTTTGCCAAGGAAATCAGCTAAATTACTACAAGCAGGAAATACCCG
TGCAACCAACTACCTCATATTCCATTCAGAATTTATACAGTTACGAGAACCAGCCCCAGC
CCAGCGATGAATGTACTCTCCTGAGTAATAAATACCTTGATGGAAATGCTAACAAATCTA
CCTCAGATGGAAGTCCTGTGATGGCCGTAATGCCTGGTACGACAGACACTATTCAAGTGC
TCAAGGGCAGGATGGATTCTGAGCAGAGCCCTTCTATTGGGTACTCCTCAAGGACTCTTG
GCCCCAATCCTGGACTTATTCTTCAGGCTTTGACTCTGTCAAACGCTAGTGATGGATTTA
ACCTGGAGCGGCTTGAAATGCTTGGCGACTCCTTTTTAAAGCATGCCATCACCACATATC
TATTTTGCACTTACCCTGATGCGCATGAGGGCCGCCTTTCATATATGAGAAGCAAAAAGG
TCAGCAACTGTAATCTGTATCGCCTTGGAAAAAAGAAGGGACTACCCAGCCGCATGGTGG
TGTCAATATTTGATCCCCCTGTGAATTGGCTTCCTCCTGGTTATGTAGTAAATCAAGACA
AAAGCAACACAGATAAATGGGAAAAAGATGAAATGACAAAAGACTGCATGCTGGCGAATG
GCAAACTGGATGAGGATTACGAGGAGGAGGATGAGGAGGAGGAGAGCCTGATGTGGAGGG
CTCCGAAGGAAGAGGCTGACTATGAAGATGATTTCCTGGAGTATGATCAGGAACATATCA
GATTTATAGATAATATGTTAATGGGGTCAGGAGCTTTTGTAAAGAAAATCTCTCTTTCTC
CTTTTTCAACCACTGATTCTGCATATGAATGGAAAATGCCCAAAAAATCCTCCTTAGGTA
GTATGCCATTTTCATCAGATTTTGAGGATTTTGACTACAGCTCTTGGGATGCAATGTGCT
ATCTGGATCCTAGCAAAGCTGTTGAAGAAGATGACTTTGTGGTGGGGTTCTGGAATCCAT
CAGAAGAAAACTGTGGTGTTGACACGGGAAAGCAGTCCATTTCTTACGACTTGCACACTG
AGCAGTGTATTGCTGACAAAAGCATAGCGGACTGTGTGGAAGCCCTGCTGGGCTGCTATT
TAACCAGCTGTGGGGAGAGGGCTGCTCAGCTTTTCCTCTGTTCACTGGGGCTGAAGGTGC
TCCCGGTAATTAAAAGGACTGATCGGGAAAAGGCCCTGTGCCCTACTCGGGAGAATTTCA
ACAGCCAACAAAAGAACCTTTCAGTGAGCTGTGCTGCTGCTTCTGTGGCCAGTTCACGCT
CTTCTGTATTGAAAGACTCGGAATATGGTTGTTTGAAGATTCCACCAAGATGTATGTTTG
ATCATCCAGATGCAGATAAAACACTGAATCACCTTATATCGGGGTTTGAAAATTTTGAAA
AGAAAATCAACTACAGATTCAAGAATAAGGCTTACCTTCTCCAGGCTTTTACACATGCCT
CCTACCACTACAATACTATCACTGATTGTTACCAGCGCTTAGAATTCCTGGGAGATGCGA
TTTTGGACTACCTCATAACCAAGCACCTTTATGAAGACCCGCGGCAGCACTCCCCGGGGG
TCCTGACAGACCTGCGGTCTGCCCTGGTCAACAACACCATCTTTGCATCGCTGGCTGTAA
AGTACGACTACCACAAGTACTTCAAAGCTGTCTCTCCTGAGCTCTTCCATGTCATTGATG
ACTTTGTGCAGTTTCAGCTTGAGAAGAATGAAATGCAAGGAATGGATTCTGAGCTTAGGA
GATCTGAGGAGGATGAAGAGAAAGAAGAGGATATTGAAGTTCCAAAGGCCATGGGGGATA
TTTTTGAGTCGCTTGCTGGTGCCATTTACATGGATAGTGGGATGTCACTGGAGACAGTCT
GGCAGGTGTACTATCCCATGATGCGGCCACTAATAGAAAAGTTTTCTGCAAATGTACCCC
GTTCCCCTGTGCGAGAATTGCTTGAAATGGAACCAGAAACTGCCAAATTTAGCCCGGCTG
AGAGAACTTACGACGGGAAGGTCAGAGTCACTGTGGAAGTAGTAGGAAAGGGGAAATTTA
AAGGTGTTGGTCGAAGTTACAGGATTGCCAAATCTGCAGCAGCAAGAAGAGCCCTCCGAA
GCCTCAAAGCTAATCAACCTCAGGTTCCCAATAGCTGAAACCGCTTTTTAAAATTCAAAA
CA
>OriGene 5' read for NM_030621 unedited
GCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNATTCGGCACGAGCCGTGCTG
CAGGTTACCTAGGGTATGAATTAATACAGACTTGGAAACTCTGAAAGAACTTAGAATCAG
CATTTTGAGAGCAGAAGCTTGGGCATGCTGTGATTTTCCAATAAACTGCTATCACAATGT
CAAAATGCAGTTCAGACAAGAGCAACACAGAGATCTCAAACATTAAAACGTAAGCTGTGC
TAGAACAAAAATGCAATGAAAGAAACACTGGATGAATGAAAAGCCCTGCTTTGCAACCCC
TCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTG
GACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATATCAGG
TTGAACTGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAG
GGAAGACATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACT
TCAGCAGAAATGGAAAAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAAC
AAGTGTCAGCTGTCAGAACTCATTCAGATCTCAAGGTTGGGGAATACTCAAACCTAGAAG
TAAATGCATCTTGGACAAAAGAGAGATGGAACCAAGAGTTTACTAAGCACCAGGTTCTCA
TTATGACTTGCTATGTCGCCTTGAATGTTTTGAAAAATGGTTACTTATCACTGTCAGACA
TTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCACCCCTATCGAGAAA
TTATGAAGCTCTGTGAAAATGTCCATCATGTCCTCGCATTTTGGGACTAAACTGCTTCAT
TNTAATGGGAAATGTGATCCAGAGGAATTGGNAGANAGATCAGAACTAA
>OriGene 3' read for NM_030621 unedited
TCTGTCACGCGGCCGCATCTATGATCGGTTTTTTTCTCTTTTTTTTTCCTTTCCAAGAGG
AAATATGAGCTAGTTTATCTAACTCAATGACCTAAATCACAGATGTATCAAAATTACGGC
AGTTTATCGCAAACGTTAAACTTTCACGGCATTAACAGCACAGCTTTGGTAGGTGAAATA
TGCATGCAACATTACTGAAAAGTATAGCAAGAAATTTATGTGAGGAGACTAAGGGTAAAG
GTGCTGTGTTTTGCTTCTTAAATAAGTAACCAATCAATTATAAAATCTGCAGCATATTTT
AGGTGTGATATGCCTAAGGTTTATTTTGTTAGAGCAACAGCCTATAAGGCAAAATTATTT
GCCATAAACATTGCCATCAGTGGGAAACTACCTGGCAGGAGGACTTTAGTAAAATGGGGC
GCTTAGCCAAAGATCATTTTTATGATAAAAAATATCCGCAGACTACATATTGCTGGGTTT
TAAAATGCCTTCAGCATACATATGACAGAAATTTTAGCCATGATCCCTGCAATAAGCTCA
CACTATACTAGTGAGTCGCAAGCATCACGCTGCCTCAACGCGCAATGTATAACACCACGA
AAGGATTGCATCACCAGCTCGCCCCAACAACATCACAGCTCAAAGACTATTCATGAAGGC
CCCACTTTTAAGGCCAGCACTCCTTCTTATGCCAGACTATGCGGTCTCCACACACCTACA
ATGCGATTCCCTCACCATGTTCGCTCATCGCCCACTCACACCCCTGTCCCTTTACTATTA
GTAGTCGCCGCTCCCGCCCACACTCTTCTCCACCCGCTTCCCCGCCCGAAGCAAACCCTC
TCCCGCCCGCCTCTCCCCCCGCGACCTCTCCCGCACCTATCGGCCCACAACTTCGCGGCT
CCGCACCCTCCGCCACCCCGCCGTCAGCGGCACACTNTACCCCCTNCTCGTCCACCGCTT
GTGCTCCGCTCTCCCCCTACTCACACCCACCTCCG
Restriction Sites NotI-NotI     
ACCN NM_030621
ORF Size 5769 bp
Insert Size 7500
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Reference Data
RefSeq NM_030621.2, NP_085124.2
RefSeq Size 10220
RefSeq ORF 5769
Locus ID 23405
Domains RIBOc, DSRM, DEAD, helicase_C, PAZ, DUF283
Protein Families Druggable Genome
Gene Summary This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
Transcript Variant: This variant (2) represents the longest transcript. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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