PIGN (NM_012327) Human Untagged Clone

CAT#: SC110315

PIGN (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIGN
• Synonyms: MCAHS; MCAHS1; MCD4; MDC4; PIG-N
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110315 sequence for NM_012327 edited (data generated by NextGen Sequencing)
  ATGCTGCTGTTCTTTACTTTGGGATTGCTTATACATTTTGTGTTCTTCGCCTCCATCTTT
  GACATTTATTTTACATCTCCTTTGGTTCATGGAATGACTCCTCAGTTTACACCATTGCCT
  CCTCCAGCGAGAAGATTAGTGTTGTTTGTTGCTGATGGCCTTCGAGCAGATGCACTTTAC
  GAATTAGATGAAAATGGAAACTCTAGAGCACCGTTTATTAGGAATATCATAATGCATGAA
  GGCAGCTGGGGCATATCTCATACACGTGTGCCAACAGAATCTCGGCCAGGTCATGTAGCT
  CTGATAGCTGGGTTTTATGAAGATGTCAGTGCAGTTGCCAAAGGATGGAAGGAAAATCCT
  GTAGAGTTTGATTCTCTTTTTAATGAAAGTAAATACACATGGAGCTGGGGAAGCCCAGAT
  ATCCTGCCTATGTTTGCCAAAGGTGCTAGTGGAGACCACGTTTATACATATAGTTATGAT
  GCTAAAAGAGAGGATTTTGGTGCTCAAGATGCAACAAAACTGGATACGTGGGTTTTTGAT
  AATGTTAAGGACTTCTTTCATCATGCCAGAAACAACCAGTCTTTGTTTTCTAAAATAAAT
  GAAGAGAAAATAGTTTTTTTCTTACATTTATTAGGAATAGATACAAACGGACATGCTCAT
  CGACCATCCTCGAGAGACTACAAGGACAATATTAAAAAAGTTGATGATGGAGTTAAAGAA
  ATCGTGTCTATGTTTAACCATTTCTATGGAAATGATGGGAAAACAACATTTATCTTTACC
  TCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCTTCAGAGACTTTA
  ACTCCTTTAGTCACTTGGGGAGCTGGAATCAAGTATCCCCAAAGAGTATCAGCTCAGCAA
  TTTGATGATGCATTTTTGAAAGAGTGGAGATTGGAGAACTGGAAGAGGCTAGATGTCAAT
  CAGGCTGATATTGCACCATTGATGACTTCCCTTATTGGAGTTCCCTTTCCTCTTAACTCA
  GTGGGAATCCTTCCTGTGGATTATCTTAACAACACTGATCTCTTCAAAGCAGAGAGCATG
  TTTACAAATGCAGTACAGATTCTTGAACAGTTCAAGGTGAAAATGACTCAGAAGAAAGAA
  GTTACTTTACCATTTTTGTTTACACCATTTAAACTGCTTTCTGATTCCAAACAGTTCAAC
  ATTTTAAGAAAAGCAAGATCTTATATAAAACACAGAAAGTTTGACGAAGTGGTCTCCCTT
  TGCAAGGAGCTAATTCATCTTGCATTGAAAGGATTGTCCTATTATCACACATATGACAGA
  TTCTTTTTGGGCGTCAATGTTGTTATTGGTTTTGTGGGATGGATATCTTATGCCTCCTTG
  TTGATCATCAAGTCTCATTCCAACCTTATAAAAGGTGTTAGTAAAGAAGTGAAGAAACCA
  AGCCATCTCCTGCCTTGTAGTTTTGTAGCTATTGGCATTTTAGTAGCATTTTTTCTGCTG
  ATTCAAGCCTGTCCCTGGACATATTATGTATATGGTTTGTTGCCACTGCCAATATGGTAT
  GCGGTTCTAAGAGAATTTCAAGTTATTCAGGACCTTGTTGTATCAGTGTTGACCTATCCT
  CTGAGCCATTTTGTTGGGTACCTGTTAGCCTTTACCCTGGGAATTGAAGTATTAGTTCTC
  AGTTTTTTCTACCGCTATATGCTTACCGCTGGACTTACTGCCTTTGCAGCTTGGCCATTT
  CTCACTCGGCTATGGACTCGAGCAAAGATGACCTCACTGAGTTGGACTTTCTTCTCTTTG
  CTCCTGGCAGTGTTCCCACTGATGCCGGTTGTAGGTCGAAAGCCAGACATCTTTCTAGTG
  ATGGGTGCAGGCTTGCTGGTTCTTCTGTTATCCCTGTGTGTTGTAACATCTCTCATGAAA
  AGAAAAGATAGCTTTATAAAGGAAGAGCTATTGGTACATCTATTACAGGTGCTGAGCACA
  GTGCTCTCCATGTATGTTGTGTATAGCACCCAGAGTAGTCTACTCAGGAAGCAAGGACTG
  CCTCTCATGAATCAAATTATTAGCTGGGCAACATTAGCCTCTTCCTTGGTTGTGCCACTA
  CTGAGTTCTCCAGTTCTCTTTCAGCGATTGTTCAGCATACTTCTTTCATTGATGTCAACC
  TACCTACTTCTAAGCACAGGGTATGAAGCTCTCTTTCCACTAGTGTTGTCTTGTTTGATG
  TTTGTCTGGATAAACATAGAACAAGAAACTCTACAACAATCTGGTGTTTGCTGTAAACAA
  AAGCTCACCAGTATCCAGTTCTCTTATAATACTGATATAACTCAGTTTCGACAGCTATAT
  CTGGATGACATCCGTAGGGCCTTTTTCCTTGTTTTCTTCTTAGTGACAGCATTTTTTGGA
  ACTGGAAATATAGCTTCTATTAACAGCTTTGATCTTGCCTCTGTCTATTGCTTTCTGACT
  GTGTTCAGTCCTTTTATGATGGGAGCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTT
  CTTGTTATGTGTGCTTTTGAAGCAGTTCAGTTGACTACTCAGTTATCGTCAAAAAGCCTT
  TTTCTCATTGTTCTCGTCATATCAGACATTATGGCTTTGCATTTTTTCTTCTTGGTCAAG
  GATTATGGCAGCTGGCTTGATATTGGGACAAGCATCAGCCAYTATGTGATTGTCATGTCC
  ATGACCATCTTTTTGGTGTTCCTCAATGGCCTGGCCCAGCTGCTCACAACGAAGAAACTC
  AGACTATGTGGCAAACCCAAAAGTCACTTCATGTGA
  
  Clone variation with respect to NM_012327.5
  685 c=>g;741 c=>t;939 t=>c;1245 t=>c;1377 t=>c;1752 g=>a;1853 c=>t;1962 g=>a;2010 t=>c;2682 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012327 unedited
  GTCAAATATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAATACCCTCC
  CGGCTATCGCTTCAGTGGGTAGAGGGGACCACTGCCCGAGAGCTTTAATGGAGCTGGGTC
  CTGCCTTCGCGCTGAGGAGCCCTCGTTTTCGAGATCAGGCCTGACCGGGATAAGCTCCAG
  CTGTTCTTGAAGACTAATTAGTTACACTGGATATGCTGCTGTTCTTTACTTTGGGATTGC
  TTATACATTTTGTGTTCTTCGCCTCCATCTTTGACATTTATTTTACATCTCCTTTGGTTC
  ATGGAATGACTCCTCAGTTTACACCATTGCCTCCTCCAGCGAGAAGATTAGTGTTGTTTG
  TTGCTGATGGCCTTCGAGCAGATGCACTTTACGAATTAGATGAAAATGGAAACTCTAGAG
  CACCGTTTATTAGGAATATCATAATGCATGAAGGCAGCTGGGGCATATCTCATACACGTG
  TGCCAACAGAATCTCGGCCAGGTCATGTAGCTCTGATAGCTGGGTTTTATGAAGATGTCA
  GTGCAGTTGCCAAAGGATGGAAGGAAAATCCTGTAGAGTTTGATTCTCTTTTTAATGAAA
  GTAAATACANCATGGAGCTNGGGGAAGCCCAGATATCCTGCCTATGTTTGCCAAAGGTGC
  TAGTGGAGACCACGTTTATACATATAGTTATGATGCTAAAAGAGAGGATTTTGGTGCTCA
  AGATGCAACAAACTGGATACCGTGNGTTTTTGANTATGTTAAGGACTTCTTTCATCATGC
  CAGAAACACCAGTCTTTTGTTTTCTAANATAATGAAGAGAAATAGTTTTTTTTCTACATT
  TATTAGAATAGATACAACGGACATGCTCATCGACATCCTCGAGAGACTACAGACATATNA
  AAAAGTTGTGATGGAGTAAGGAATCGGGTCTTGTTTACCATTCTATGGATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012327 unedited
  GGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTGCAAACACTTTTTTTATTGAA
  GCTCTTGAAAAATACACCCTGACTGTCTAACAAAACCAGTGCTGAAAGCTGATAAATATT
  GACGGAGGCACCTGGTGATTTCCTTCCAGCTCAGCCACAGCAATGACTCTGGATGCCATC
  AGCACTGCCCCCACAGGCATATGCTCTCCTCTGGGTATGTGGGAAGTTGTGCGACTGGGG
  ACCAGATTGCTGCAGGTGCTCTCAACATCACTGGGAAGAGCTGCCAGCCAAAGGAAAAAA
  ATGAAAAAGGAGAATGCCTTCCTATGCTTTTCCACAGATATAATCACTATTTCATGCCTG
  CAAAACCTATAAAAAAAAAAGAAGCTCCTTTGTTCCAGATAACCTGTCAATTCGGAATTC
  CAAATACCATTTTCCTTACAGGAGTAGAATATACTATATATCCATATCCATCTTCTTATT
  GAAGCCTTGATGAGATTTTAGCTTAAAAGGAGAATCAGGATCCAGATGCTGAATGGTGCT
  TCAGCAACCTCACATGAAGTGACTTTTGGGTTTGCCACATAGTCTGAGTTTCTTCGTTGT
  GAGCAGCTGGGCCAGGCCATTGAGGAACACCAAAAAGATGGTCATGGACATGACANTCAC
  ATAATGGCTGATGCTTGTCCCAATATCAAGCCAGCTGCCATAATCCTTGACCAAGAAGAA
  AAAATGCACAGCCATAATGCCTGATATGACGAGAACAATGAGAAAAAAGCTCTTTGACGA
  TAACTGAGTAGTCAACCTGACCTGCCTCATAGCACACCTATCATGACACAAGGGACTAAA
  ATCCTTCACATTTCACGCCTCCCATCATAAAAGACTGAACACAGTCCGAAGCAATAGACG
  AAGCCAGATCAAAGCTGTTAATAGAAGCTTATTTCCAGTCCCAAAATGCTGTCACTATAT
  TAATACAGNATAAGCCCTCACGAGCCTCCAGATAGCTGCGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012327
• ORF Size: 2796 bp
• Insert Size: 3520
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_012327.3, NP_036459.1
• RefSeq Size: 4751
• RefSeq ORF: 2796
• Locus ID: 23556
• Domains: PigN
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor. Two alternatively spliced variants, which encode an identical isoform, have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform.