MRPL42 (NM_014050) Human Untagged Clone

CAT#: SC110399

MRPL42 (untagged)-Human mitochondrial ribosomal protein L42 (MRPL42), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL42
• Synonyms: HSPC204; L31MT; L42MT; MRP-L31; MRP-L42; MRP-S32; MRPL31; MRPS32; PTD007; RPML31; S32MT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110399 sequence for NM_014050 edited (data generated by NextGen Sequencing)
  ATGGCTGTAGCTGCAGTAAAATGGGTGATGTCAAAGAGAACTATCTTGAAACATTTATTT
  CCAGTCCAAAATGGAGCTTTATATTGTGTTTGTCATAAATCTACGTATTCTCCTCTACCA
  GATGACTATAATTGCAACGTAGAGCTTGCTCTGACTTCTGATGGCAGGACAATAGTATGC
  TACCACCCTTCTGTGGACATTCCATATGAACACACAAAACCTATCCCTCGGCCAGATCCT
  GTGCATAATAATGAAGAAACACATGATCAAGTGCTGAAAACCAGATTGGAAGAAAAAGTT
  GAACACCTTGAGGAAGGACCTATGATAGAACAACTTAGCAAAATGTTCTTTACTACTAAG
  CACCGTTGGTATCCTCATGGACGGTATCACAGATGTCGTAAGAATCTGAATCCTCCAAAA
  GACAGATGA
  
  Clone variation with respect to NM_014050.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014050 unedited
  GTTACGATTATGTNATACGACTTCACTATAGGGCGGNCGCGCAATTCGCACGAGGGTGAG
  AAGCCGTCAAGGAGTAGAAATTGGTATGCTTACAAGCAGATTCTAAAAGCAGTTTCTCTT
  CAGAACATCTTTTTTCATACCACTTGATAAGCATCTTGAAACACCATGGCTGTAGCTGCA
  GTAAAATGGGTGATGTCAAAGAGAACTATCTTGAAACATTTATTTCCAGTCCAAAATGGA
  GCTTTATATTGTGTTTGTCATAAATCTACGTATTCTCCTCTACCAGATGACTATAAATTG
  CAACGTAGAGCTTGCTCTGACTTCTGATGGCAGGACAATAGTATGCTACCACCCTTCTGT
  GGACATTCCATATGAACACACAAAACCTATCCCTCGGCCAGATCCTGTGCATAATAATGA
  AGAAACACATGATCAAGTGCTGAAAACCAGATTGGAAGAAAAAGTTGAACACCTTGAGGA
  AGGACCTATGATAGAACAACTTAGCAAAATGTTCTTTACTACTAAGCACCGTTGGTATCC
  TCATGGACGGTATCACAGATGTCGTAAGAATCTGAATCCTCCAAAAGACAGATGATGCGG
  AGGTTCCTGGGGGAATCAAAGAGAAATGTGCCTCATTTGCCATTTGAGAAAATGCAGTCT
  GGTGTATTCAGTAATATATAGTAAAGTAATAATGATAAAATATCTTTTCATATATTAGAA
  TGTGTACTTTTATATAAAGTAATTCTGGATTTGACATTCTCATTTAGAAGAACCTATTTT
  CCTTTTTCCTTTTCTATTTTAGGGNTTCATTTATGTGCCGCCTCCCAATTAGACTTTTCC
  ATAGTGCCAAGCCCTACATATCAGTACAACTCTATAAATACATCAGAAATTCACACTCTA
  TTATTAAACGGCCCTTGTGCTAGATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014050 unedited
  GGACCGGGGAGCGGNNTCATAAAGTCGAGAGACGTCTTTTTTTTTTTTTTTTTTTTTTTT
  TCAGGAAAGCCATGCACATTTTTATTAATCATATTTATACTACTAACAATGCTTGGCTGA
  TGATTAAAAATACTTAAATCACAGTAAACACACAAAAAGAGATGTTTCTCAACTTATCAT
  AGGGTTACTTCCCAATAAACCTACTATCAGTTGAAAATATTGTAAATTGAAAATGCTACC
  CTGTCTTAAATATGCTCAAAACACTTACAGTAGCCTACTGTTTAGCAAAATTATCTAACA
  CGAAGCCCGTTTTATAATAAAGCGTTGAATTTCTGAGGTAATTAATTGATGCCCTACTGA
  ATATGTATGGCTCTGGCACTATGGAAAACGCCTAACTTGGACACCGCCCATAACTGAACC
  CCTCTTATCCACTACGAAAACACACAATAGGTTTCTCTAAATGAAAAGGTCAACACCAGA
  ATTCCTTTATTTAAAAGTCCACCTTCTCATCCATGAAAACTAATTTTATCCTTATCACTT
  CACTATACTTCACCGAATACACCATACTGCTTTTCTCCAAATGGCACAGAGGCACATTTC
  TCTTTAATCCCCACAACACCCTACAAACTATCTGTCTTTTGGAGGATTCAAATTCTTACA
  ACATCTGAGAAACCGCCCATAAGGAATACAAATGGCCGCTTAAAAAATAACAAAATTTAG
  CCAAAGCCGTTCTATCTTACGCCCTTCCTCAAGGGGCCAATTTCTACTCCCAACTGGTTT
  CCAACCTTGTTAATTGTTCTCTTTTTATATCCCACCAATCGGCCCCAAGAAAGCCTCCCG
  TGTAACTATGACTCTACCACAAGGCGGACAACACTCTTGTCTCACCCTTACTTATCACAC
  CGCTCCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014050
• ORF Size: 429 bp
• Insert Size: 1180
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_014050.2, NP_054769.1
• RefSeq Size: 2070
• RefSeq ORF: 429
• Locus ID: 28977
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq, May 2011]
  Transcript Variant: This variant (1) encodes the supported protein. Both variants 1 and 2 encode the same protein.