TMS1 (PYCARD) (NM_145182) Human Untagged Clone

CAT#: SC110404

PYCARD (untagged)-Human PYD and CARD domain containing (PYCARD), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYCARD
• Synonyms: ASC; CARD5; TMS; TMS-1; TMS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_145182 edited
  GAATTCGGCACGAGGGGGGTGAGCGGCGGCAGCGGCCGGGGATCCTGGAGCCATGGGGCG
  CGCGCGCGACGCCATCCTGGATGCGCTGGAGAACCTGACCGCCGAGGAGCTCAAGAAGTT
  CAAGCTGAAGCTGCTGTCGGTGCCGCTGCGCGAGGGCTACGGGCGCATCCCGCGGGGCGC
  GCTGCTGTCCATGGACGCCTTGGACCTCACCGACAAGCTGGTCAGCTTCTACCTGGAGAC
  CTACGGCGCCGAGCTCACCGCTAACGTGCTGCGCGACATGGGCCTGCAGGAGATGGCCGG
  GCAGCTGCAGGCGGCCACGCACCAGGGCTCTGGAGCCGCGCCAGCTGGGATCCAGGCCCC
  TCCTCAGTCGGCAGCCAAGCCAGGCCTGCACTTTATAGACCAGCACCGGGCTGCGCTTAT
  CGCGAGGGTCACAAACGTTGAGTGGCTGCTGGATGCTCTGTACGGGAAGGTCCTGACGGA
  TGAGCAGTACCAGGCAGTGCGGGCCGAGCCCACCAACCCAAGCAAGATGCGGAAGCTCTT
  CAGTTTCACACCAGCCTGGAACTGGACCTGCAAGGACTTGCTCCTCCAGGCCCTAAGGGA
  GTCCCAGTCCTACCTGGTGGAGGACCTGGAGCGGAGCTGAGGCTCCTTCCCAGCAACACT
  CCGGTCAGCCCCTGGCAATCCCACCAAATCATCCTGAATCTGATCTTTTTATACACAATA
  TACGAAAAGCCAGCTTGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145182 unedited
  GTTCAAAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGTG
  AGCGGCGGCAGCGGCCGGGGATCCTGNGACCATGGGGCGCGCGCGCGACGCCATCCTGGA
  TGCGCTGGAGAACCTGACCGCCGAGGAGCTCAAGAAGTTCAAGCTGAAGCTGCTGTCGGT
  GCCGCTGCGCGAGGGCTACGGGCGCATCCCGCGGGGCGCGCTGCTGTCCATGGACGCCTT
  GGACCTCACCGACAAGCTGGTCAGCTTCTACCTGGAGACCTACGGCGCCGAGCTCACCGC
  TAACGTGCTGCGCGACATGGGCCTGCAGGAGATGGCCGGGCAGCTGCAGGCGGCCACGCA
  CCAGGGCTCTGGAGCCGCGCCAGCTGGGATCCAGGCCCCTCCTCAGTCGGCAGCCAAGCC
  AGGCCTGCACTTTATAGACCAGCACCGGGCTGCGCTTATCGCGAGGGTCACAAACGTTGA
  GTGGCTGCTGGATGCTCTGTACGGGAAGGTCCTGACGGATGAGCAGTACCAGGCAGTGCG
  GGCCGAGCCCACCAACCCAAGCAAGATGCGGAAGCTCTTCAGTTTCACACCAGCCTGGAA
  CTGGACCTGCAAGGACTTGCTCCTCCAGGCCCTAAGGGAGTCCCAGTCCTACCTGGTGGA
  GGACCTGGAGCGGAGCTGAGGCTCCTTCCCACAACACTCCGGTCAGCCCCTGGCAATCCC
  ACCAAATCATCCTGAATCTGATCTTTNTATACACAATATACGAAAAGCCAGCTTGAAAAA
  AAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAAAGATCC
  CNGGTGGCATCCCTGTGACCCTNNCCGTGCCTCTTCTGGCCCTGGAAAGTGCACTNCAGT
  GCCACCANNCTGTCTAATAAAATAAGTGCATCATTGCTGACTAGTGTCTATATATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145182 unedited
  TTTGGGTATTTTATGAACCGCGCCGCATTCTANNGATCGGTTTTTTTTTTTTTTTTTTCA
  AGCTGGCTTTTCGTATATTGTGTATAAAAAGATCAGATTCAGGATGATTTGGTGGGATTG
  CCAGGGGCTGACCGGAGTGTTGCTGGGAAGGAGCCTCAGCTCCGCTCCAGGTCCTCCACC
  AGGTAGGACTGGGACTCCCTTAGGGCCTGGAGGAGCAAGTCCTTGCAGGTCCAGTTCCAG
  GCTGGTGTGAAACTGAAGAGCTTCCGCATCTTGCTTGGGTTGGTGGGCTCGGCCCGCACT
  GCCTGGTACTGCTCATCCGTCAGGACCTTCCCGTACAGAGCATCCAGCAGCCACTCAACG
  TTTGTGACCCTCGCGATAAGCGCAGCCCGGTGCTGGTCTATAAAGTGCAGGCCTGGCTTG
  GCTGCCGACTGAGGAGGGGCCTGGATCCCAGCTGGCGCGGCTCCAGAGCCCTGGTGCGTG
  GCCGCCTGCAGCTGCCCGGCCATCTCCTGCAGGCCCATGTCGCGCAGCACGTTAGCGGTG
  AGCTCGGCGCCGTAGGTCTCCAGGTAGAAGCTGACCAGCTTGTCGGTGAGGTCCAAGGCG
  TCCATGGACAGCAGCGCGCCCCGCGGGATGCGCCCGTAGCCCTCGCGCAGCGGCACCGAC
  CGCAGCTTCAGCTTGAACTTCTTGAGCTCCTCGGCGGTCAGGTTCTCCAGCGCATCCAGG
  AAGGCGTCGCGCGCGCGCCCCATGGCTCCAGGATCCCCGGCCGGTGGCGCCGCTCACCCC
  CCTTGACTCGAATTCGCGGCCGCCCTATTAGGAGTCGATTACAAAATTCTGACGGTTCAC
  TAAACGAGCTCTGCTTTTATAGAACCTCCCCCGGTACCACCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145182
• ORF Size: 531 bp
• Insert Size: 780
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_145182.1, NP_660183.1
• RefSeq Size: 879
• RefSeq ORF: 531
• Locus ID: 29108
• Domains: PAAD_DAPIN
• Protein Families: Druggable Genome
• Protein Pathways: Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway
• Gene Summary: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an internal coding exon compared to variant 1. It maintains the same reading frame, and encodes a shorter isoform (b) missing a segment of 19 aa, compared to isoform a.