ARFGAP1 (NM_018209) Human Untagged Clone

CAT#: SC110523

ARFGAP1 (untagged)-Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARFGAP1
• Synonyms: ARF1GAP; HRIHFB2281
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110523 sequence for NM_018209 edited (data generated by NextGen Sequencing)
  ATGGCCAGCCCAAGAACCAGGAAGGTTCTTAAAGAAGTCAGGGTGCAGGATGAGAACAAC
  GTTTGTTTTGAGTGTGGCGCGTTCAATCCTCAGTGGGTCAGTGTGACCTACGGCATCTGG
  ATCTGCCTGGAGTGCTCGGGGAGACACCGCGGGCTTGGGGTTCACCTCAGCTTTGTGCGC
  TCTGTTACTATGGACAAGTGGAAGGACATTGAGCTTGAGAAGATGAAAGCTGGTGGGAAT
  GCTAAGTTCCGAGAGTTCCTGGAGTCTCAGGAGGATTACGATCCTTGCTGGTCCTTGCAG
  GAGAAGTACAACAGCAGAGCCGCGGCCCTCTTTAGGGATAAGGTGGTCGCTCTGGCCGAA
  GGCAGAGAGTGGTCTCTGGAGTCATCACCTGCCCAGAACTGGACCCCACCTCAGCCCAGG
  ACGCTGCCGTCCATGGTGCACCGAGTCTCTGGCCAGCCGCAGAGTGTGACCGCCTCCTCG
  GACAAGGCTTTTGAAGACTGGCTGAATGATGACCTCGGCTCCTATCAAGGGGCCCAGGGG
  AATCGCTACGTGGGGTTTGGGAACACGCCACCGCCTCAGAAGAAAGAAGATGACTTCCTC
  AACAACGCCATGTCCTCCCTGTACTCGGGCTGGAGCAGCTTCACCACTGGAGCCAGCCGG
  TTTGCCTCGGCAGCCAAGGAGGGCGCTACAAAGTTTGGATCCCAAGCGAGTCAGAAGGCG
  TCCGAGCTGGGCCACAGCCTGAACGAGAACGTCCTCAAGCCTGCGCAGGAGAAGGTGAAG
  GAGGGAAAGATTTTTGATGATGTCTCCAGTGGGGTCTCTCAGTTGGCGTCCAAGGTCCAG
  GGAGTCGGTAGTAAGGGATGGCGGGACGTCACCACCTTTTTTTCGGGGAAAGCAGAGGGC
  CCCTTGGACAGCCCCTCGGAGGGCCACAGTTATCAGAACAGCGGTCTGGACCACTTCCAA
  AACAGCAACATAGACCAGAGCTTCTGGGAGACCTTTGGAAGTGCTGAGCCCACCAAGACC
  CGCAAGTCCCCGAGCAGCGACAGCTGGACGTGCGCGGACACCTCCACCGAGAGGAGGAGC
  TCGGACAGCTGGGAGGTGTGGGGCTCGGCCTCCACCAACAGGAACAGCAACAGCGACGGC
  GGGGAGGGCGGGGAGGGCACCAAGAAGGCAGTGCCGCCGGCCGTGCCCACTGATGATGGC
  TGGGACAACCAGAACTGGTAG
  
  Clone variation with respect to NM_018209.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018209 unedited
  NCATTTACCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGGGCGACCGGGCGCATCCTCATTGCAGTGCGGCGGCCCTACCTC
  GGCCCTGGCCTGACCCCGGCGGCCCTGCCCGCCCCTCCCTCCAGCATCATGGCCAGCCCA
  AGAACCAGGAAGGTTCTTAAAGAAGTCAGGGTGCAGGATGAGAACAACGTTTGTTTTGAG
  TGTGGCGCGTTCAATCCTCAGTGGGTCAGTGTGACCTACGGCATCTGGATCTGCCTGGAG
  TGCTCGGGGAGACACCGCGGGCTTGGGGTTCACCTCAGCTTTGTGCGCTCTGTTACTATG
  GACAAGTGGAAGGACATTGAGCTTGAGAAGATGAAAGCTGGTGGGAATGCTAAGTTCCGA
  GAGTTCCTGGAGTCTCAGGAGGATTACGATCCTTGCTGGTCCTTGCAGGAGAAGTACAAC
  AGCAGAGCCGCGGCCCTCTTTAGGGATAAGGTGGTCGCTCTGGCCGAAGGCAGAGAGTGG
  TCTCTGGAGTCATCACCTGCCCAGAACTGGACCCCACCTCAGCCCAGGACGCTGCCGTCC
  ATGGTGCACCGAGTCTCTGGCCAGCCGCAGAGTGTGACCGCCTCCTCGGACAGGCTTTTG
  AAGACTGGCTGAATGATGACCTCGGCTCCTATCAAGGGGCCCAGNGGAATCGCTACGTGG
  GGGNTTGGNGAACACGCCACCGCCTCAGAAGAAAGAAGATGACTTNCTCAACACGCCATG
  TCCCTCCTGTACTCGGNCTGGNAGCAGCTCCCACTGGAGCCCAGCCGGNTTGCTCGCAGC
  CAGGNAGGCGCTACAAAGTTGGATCCCAGCGATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018209 unedited
  CGGACCATAGGNGATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGGTCACAGGG
  NATGCCACCCGGGATCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGT
  TTTTTTTTTTTTTTTTTGCATGTTCCCGTTTGCTTTATTGGAATGCTGTCAGGTCCGCGC
  CTTCCACCTGGGCCCTCACACACAGCAAGGAGAGGCCCCAGCACCGGTCCCAGGCCCACC
  TGCCCGCACCTGCAGAAGGGAAAGGCCATCANCCCTTCCGTGGACGGGGTCTCGGCCACC
  ACATCCACCTTCTGAAGGTGGCCCAGACACCTCCACGCTGCTGACTGCACTTCCCATCAA
  AAGGGACTCCCTGGGGCAGAGTGGGCCGTCCCCCTACCCCCGAGGAAGGCACCCTCCTGG
  GCGGGGACAGACCTTGGCTCCATGCTGCCAAGTCAAGGTGCTGGCGTGGAAACAGGCACA
  TGTGGAACACCACATCCCACTGTCCAAGGTGGAGTCCACCCCTCCTCGGAGCACTCAGCC
  CACCAGGGTCCAAGCAGCCCTCGGGAGATACCACGCGGCCGCCCACGTCTCAGCTTGGAG
  AAAGACACTCAAAGACCCCCACGCTCAGCTCTCGGGGGCTGGCTTCCTGGGCCCTTTGTG
  CTGGCTGCTTTCCCGGTGGCTTCTCAGCACCTTGAGCCTCTGATGCCCCGCCTCTGACCT
  CAGGTCCAACTACAGCAGGTGGCTGCTTCGGTGATCAAATCCCTCCGTGGCAGCCAAAGA
  CACCGGGCCCTCCACACCAGCTACCAAGAGGGCTGGTGCCCACAGGGAGGACCGGGNCGC
  CAGNAGGGCTCGCTGAAATCTTCGTCCCATCCTGGTCTGGCAGTGGCTGAATCCCCTAGG
  TGGGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018209
• ORF Size: 1221 bp
• Insert Size: 2900
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_018209.2, NP_060679.1
• RefSeq Size: 3250
• RefSeq ORF: 1221
• Locus ID: 55738
• Domains: ArfGap
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) lacks an in-frame exon and uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. The encoded isoform (a) is shorter, compared to isoform b.