Adenylosuccinate Lyase (ADSL) (NM_000026) Human Untagged Clone

CAT#: SC110822

ADSL (untagged)-Human adenylosuccinate lyase (ADSL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADSL
• Synonyms: AMPS; ASASE; ASL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000026, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCTGGAGGCGATCATGGTTCGCCCGACAGCTACCGCTCACCTCTTGCCTCCCGCTATGCCAGCC
  CGGAGATGTGCTTCGTGTTTAGCGACAGGTATAAATTCCGGACATGGCGGCAGCTGTGGCTGTGGCTGGC
  GGAGGCCGAGCAGACATTGGGTTTGCCTATCACAGATGAACAAATCCAGGAGATGAAATCAAACCTGGAG
  AACATCGACTTCAAGATGGCAGCTGAGGAAGAGAAACGTTTACGACATGATGTGATGGCTCACGTGCACA
  CATTTGGCCACTGCTGTCCAAAAGCTGCAGGCATTATTCACCTTGGTGCTACTTCTTGCTATGTTGGAGA
  CAATACTGACTTGATTATTCTTAGAAATGCACTTGACCTGCTTTTGCCAAAGCTTGCCAGAGTGATCTCT
  CGGCTTGCCGACTTTGCTAAGGAACGAGCCAGTCTACCCACATTAGGTTTCACACATTTCCAGCCTGCAC
  AGCTGACCACAGTTGGGAAACGTTGCTGTCTTTGGATTCAGGATCTTTGCATGGATCTCCAGAACTTGAA
  GCGTGTCCGAGATGACCTGCGCTTCCGGGGAGTAAAGGGTACCACTGGCACTCAGGCCAGTTTCCTGCAG
  CTCTTTGAGGGAGATGACCATAAGGTAGAGCAGCTTGACAAGATGGTGACAGAAAAGGCAGGATTTAAGA
  GAGCTTTCATCATCACAGGGCAGACATATACACGAAAAGTGGATATTGAAGTACTGTCTGTGCTGGCTAG
  CTTGGGGGCATCAGTGCACAAGATTTGCACCGACATACGCCTCCTGGCAAACCTCAAGGAGATGGAGGAA
  CCCTTTGAAAAACAGCAGATTGGCTCAAGTGCGATGCCATATAAGCGGAATCCCATGCGTTCAGAACGTT
  GCTGCAGTCTTGCCCGCCACCTGATGACCCTTGTCATGGACCCGCTACAGACAGCATCTGTCCAGTGGTT
  TGAACGCACACTGGATGATAGTGCCAACCGACGGATCTGTTTGGCCGAGGCATTTCTTACCGCAGATACT
  ATATTGAATACGCTGCAGAACATTTCTGAAGGATTGGTCGTGTACCCCAAAGTAATTGAACGGCGCATTC
  GGCAAGAGCTGCCTTTCATGGCCACAGAGAACATCATCATGGCCATGGTCAAAGCTGGAGGTAGCCGCCA
  GGATTGCCATGAGAAAATCAGAGTGCTTTCTCAGCAGGCAGCTTCTGTGGTTAAGCAGGAAGGGGGTGAC
  AATGACCTCATAGAGCGTATCCAGGTTGATGCCTACTTCAGTCCCATTCACTCCCAGTTGGATCATTTAC
  TGGATCCTTCTTCTTTCACTGGTCGTGCCTCCCAGCAGGTGCAGAGATTCTTAGAAGAGGAGGTGTATCC
  CCTGTTAAAACCATATGAAAGCGTGATGAAGGTGAAAGCAGAATTATGTCTGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000026 unedited
  CGTCAGATTTGAACTCGACTCATATAGGCGGCCGCTNAATCGGCACGAGGCCTCGTGCCG
  AATTCGGCACGAGGCAGGGTTGGGATGGCGGCTGGAGGCGATCATGGTTCGCCCGACAGC
  TACCGCTCACCTCTTGCCTCCCGCTATGCCAGCCCGGAGATGTGCTTCGTGTTTAGCGAC
  AGGTATAAATTCCGGACATGGCGGCAGCTGTGGCTGTGGCTGGCGGAGGCCGAGCAGACA
  TTGGGTTTGCCTATCACAGATGAACAAATCCAGGAGATGAAATCAAACCTGGAGAACATC
  GACTTCAAGATGGCAGCTGAGGAAGAGAAACGTTTACGACATGATGTGATGGCTCACGTG
  CACACATTTGGCCACTGCTGTCCAAAAGCTGCAGGCATTATTCACCTTGGTGCTACTTCT
  TGCTATGTTGGAGACAATACTGACTTGATTATTCTTAGAAATGCACTTGACCTGCTTTTG
  CCAAAGCTTGCCAGAGTGATCTCTCGGCTTGCCGACTTTGCTAAGGAACGAGCCAGTCTA
  CCCACATTAGGTTTCACACATTTCCAGCCTGCACAGCTGACCACAGTTGGGAAACGTTGC
  TGTCTTTGGATTCAGGATCTTTGCATGGATCTCCAGAACTTGAAGCGTGTCCGAGATGAC
  CTGCGCTTCCGGGGAGTAAAGGGTACCACTGGCACTCAGGCCAGTTTCCTGCAGCTCTTT
  GAGGGAGATGACCATAANGTAGAGCAGCTTGACTAGATGGTGACAGACAAGGCAGGATTT
  TAGAGAGCTTTCATCATCACAGGGCAGACATATACACGAAAAGTGGATAATGCAGTACTG
  TCTGTGCTGGCTAGCTTGNGGNCATCAGTGCACAGATTTGCACTGACTACCCCTCTGCCA
  AACTCAGGAGATGTAGCACCCTTTGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000026 unedited
  TCGACATATACAATGATTTTCGTTTAATTCTCTTCCAACTCTACATACATAATTCTGCTT
  TCACCTTCATCACGCTTTCATATGGTTTTAACAGGGGATACACCTCCTCTTCTAAGAATC
  TCTGCACCTGCTGGGAGGCACGACCAGTGAAAGAAGAAGGATCCAGTAAATGATCCAACT
  GGGAGTGAATGGGACTGAAGTAGGCATCAACCTGGATACGCTCTATGAGGTCATTGTCAC
  CCCCTTCCTGCTTAACCACAGAAGCTGCCTGCTGAGAAAGCACTCTGATTTTCTCATGGC
  AATCCTGGCGGCTACCTCCAGCTTTGACCATGGCCATGATGATGTTCTCTGTGGCCATGA
  AAGGCAGCTCTTGCCGAATGCGCCGTTCAATTACTTTGGGGTACACGACCAATCCTTCAG
  AAATGTTCTGCAGCGTATTCAATATAGTATCTGCGGTAAGAAATGCCTCGGCCAAACAGA
  TCCGTCGGTTGGCACTATCATCCAGTGTGCGTTCAAACCACTGGACAGATGCTGTCTGTA
  GCGGGTCCATGACAAGGGTCATCATGTGGCGGGCAAGACTGCAGCAACGTTCTGAACGCA
  TGGGATTCCGCTTATATGGCATCGCACTTGAGCCAATCTGCTGTTTTTCAAAGGGTTCCT
  CCATCTNCTTGAGGTTGCCAGGAGGCGTATGTCGGTGCAAATCTTGTGCACTGATGCCCC
  CAAGCTAGCCAGCACAGACAGTACTTAATATCCACTTTCCTGTATATGTCTGCCCTGTGA
  TGATGATAGCTCTCTTTAATCCTGCCTTTTCTGTCACCATCTTGTCAAGCTGCTCTACCT
  TATGGTCATTTCCCTAAAGGACTGCAAGAAACTGGCCCTGATGCCATTGTTTCCCTTTTC
  TCCCAGGATCGCAGTTATCTCCGGCACGCTTAATGTCTGGAAATCCTCCAAATTCCTGAT
  CCAAACACGTACGTTCCAACTGGGGCACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000026
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000026.1, NP_000017.1
• RefSeq Size: 1692 bp
• RefSeq ORF: 1455 bp
• Locus ID: 158
• Cytogenetics: 22q13.1
• Domains: lyase_1
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the lyase 1 family. It is an essential enzyme involved in purine metabolism, and catalyzes two non-sequential reactions in the de novo purine biosynthetic pathway: the conversion of succinylaminoimidazole carboxamide ribotide (SAICAR) to aminoimidazole carboxamide ribotide (AICAR) and the conversion of adenylosuccinate (S-AMP) to adenosine monophosphate (AMP). Mutations in this gene are associated with adenylosuccinase deficiency (ADSLD), a disorder marked with psychomotor retardation, epilepsy or autistic features. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (a).