Apolipoprotein A I (APOA1) (NM_000039) Human Untagged Clone

CAT#: SC110828

APOA1 (untagged)-Human apolipoprotein A-I (APOA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APOA1
• Synonyms: apo(a); HPALP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110828 sequence for NM_000039 edited (data generated by NextGen Sequencing)
  ATGAAAGCTGCGGTGCTGACCTTGGCCGTGCTCTTCCTGACGGGGAGCCAGGCTCGGCAT
  TTCTGGCAGCAAGATGAACCCCCCCAGAGCCCCTGGGATCGAGTGAAGGACCTGGCCACT
  GTGTACGTGGATGTGCTCAAAGACAGCGGCAGAGACTATGTGTCCCAGTTTGAAGGCTCC
  GCCTTGGGAAAACAGCTAAACCTAAAGCTCCTTGACAACTGGGACAGCGTGACCTCCACC
  TTCAGCAAGCTGCGCGAACAGCTCGGCCCTGTGACCCAGGAGTTCTGGGATAACCTGGAA
  AAGGAGACAGAGGGCCTGAGGCAGGAGATGAGCAAGGATCTGGAGGAGGTGAAGGCCAAG
  GTGCAGCCCTACCTGGACGACTTCCAGAAGAAGTGGCAGGAGGAGATGGAGCTCTACCGC
  CAGAAGGTGGAGCCGCTGCGCGCAGAGCTCCAAGAGGGCGCGCGCCAGAAGCTGCACGAG
  CTGCAAGAGAAGCTGAGCCCACTGGGCGAGGAGATGCGCGACCGCGCGCGCGCCCATGTG
  GACGCGCTGCGCACGCATCTGGCCCCCTACAGCGACGAGCTGCGCCAGCGCTTGGCCGCG
  CGCCTTGAGGCTCTCAAGGAGAACGGCGGCGCCAGACTGGCCGAGTACCACGCCAAGGCC
  ACCGAGCATCTGAGCACGCTCAGCGAGAAGGCCAAGCCCGCGCTCGAGGACCTCCGCCAA
  GGCCTGCTGCCCGTGCTGGAGAGCTTCAAGGTCAGCTTCCTGAGCGCTCTCGAGGAGTAC
  ACTAAGAAGCTCAACACCCAGTGA
  
  Clone variation with respect to NM_000039.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000039 unedited
  GGATTTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGAGGAGGTCCCCCAC
  GGCCCTTCAGGATGAAAGCTGCGGTGCTGACCTTGGCCGTGCTCTTCCTGACGGGGAGCC
  AGGCTCGGCATTTCTGGCAGCAAGATGAACCCCCCCAGAGCCCCTGGGATCGAGTGAAGG
  ACCTGGCCACTGTGTACGTGGATGTGCTCAAAGACAGCGGCAGAGACTATGTGTCCCAGT
  TTGAAGGCTCCGCCTTGGGAAAACAGCTAAACCTAAAGCTCCTTGACAACTGGGACAGCG
  TGACCTCCACCTTCAGCAAGCTGCGCGAACAGCTCGGCCCTGTGACCCAGGAGTTCTGGG
  ATAACCTGGAAAAGGAGACAGAGGGCCTGAGGCAGGAGATGAGCAAGGATCTGGAGGAGG
  TGAAGGCCAAGGTGCAGCCCTACCTGGACGACTTCCAGAAGAAGTGGCAGGAGGAGATGG
  AGCTCTACCGCCAGAAGGTGGAGCCGCTGCGCGCAGAGCTCCAAGAGGGCGCGCGCCAGA
  AGCTGCACGAGCTGCAAGAGAAGCTGAGCCCACTGGGCGAGGAGATGCGCGACCGCGCGC
  GCGCCCATGTGGACGCGCTGCGCACGCATCTGGCCCCCTACAGCGACGAGCTGCGCCAGC
  GCTTGGCCGCGCGCCTTGAGGCTCTCAAGGAGAACGGCGGCGCCAGACTGGCCGAGTACC
  ACGCCAAGGCCACCGAGCATCTGAGCACGCTCAGCGAGAAGGCCAAGCCCGCGCTCGAGG
  ACCTNCGCCAAGCCTGCTGCCCGTGCTGGAGAGCTTCAGGTCAGCTTCCTGAGCGCTCTC
  GAGGGAGTACACTAGAGCTCACACCCAGTGAGGCGCCCNGCGCGCCCTTNNNCGTGCTAG
  AATAAACGTTCCAAGTGGAAAAAAAAAAAAAAAAAAAAAACTCACTTAATGCGNCGGNCA
  TACTGTTCTGACGACCGGGTGGATCTTGGACCTCCAGGCTTCGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000039 unedited
  GCCATTGGNGATGGCACTNTCCAGGTCCAGNANAGCACTGGGGNAGGGTCACAGGGATGC
  CACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCACTTTGGAAACGTTTATTCTGAGCACC
  GGGAAGGGGGGCGGCGGCGGGCCCCTCACTGGGTGTTGAGCTTTTTAGTGTACTCCTCGA
  GAGCGCTCAGGAAGCTGACCTTGAAGCTCTCCAGCACGGGCAGCAGGCCTTGGCGGAGGT
  CCTCGAGCGCGGGCTTGGCCTTCTCGCTGAGCGTGCTCAAATGCTCGGTGGCCTTGGCGT
  GGTACTCGGCCAGTCTGGCGCCGCCGTTCTCCTTGAGAGCCTCAAGGCGCGCGGCCAAGC
  GCTGGCGCAGCTTGTCTCTGTAGGGGGCCAAATGCGTGCGCAGCGCGTCCACATGGGCGC
  GCGCGCGGTCTCGCATCTCCTTTCCCAGTGGGCTCAGCTTCTTTTGCAGCTCGTGCAGTT
  TCTGGCGCGCGCCCTCTTGGAGCTCTGCGCGCAGCGGCTCCATCTTTTGGCGGGAGAGCT
  CCATCTCCTCCTGCCACTTTTTTTGGAAGTCTTCCAGGTAGGGTTGAACTTTGGCCTTCA
  CCCCCTCCAAATTCTTGGTCATCTCCTGCCCTAGGCCCTCTGTCTTCTTTTTCAGGTTAT
  CCCAAAACTTCTGGGTCACAAGGGCCGAACTTGTTCCCGCAACTTGTTGAAAGGGGGAGG
  TCACCCTCTTCCATTTTGCAAAGGAGCCTTTAAGTTTAACTTGTTTCCCAAGGCGGGGCC
  CTTAAACTGGGAAAAAATTCCTCTGCCCTGTTTTTTACCCATCCACTTCCGGTGGCCAGT
  TTCTTCACATCATCCCGGGGTTTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000039
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000039.1, NP_000030.1
• RefSeq Size: 897 bp
• RefSeq ORF: 804 bp
• Locus ID: 335
• Cytogenetics: 11q23.3
• Domains: Apolipoprotein
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: PPAR signaling pathway
• Gene Summary: 'This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform. Variants 1-3 encode the same isoform (1).