Apolipoprotein A I (APOA1) (NM_000039) Human Untagged Clone

CAT#: SC110828

APOA1 (untagged)-Human apolipoprotein A-I (APOA1)


  "NM_000039" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol APOA1
Synonyms apo(a); HPALP2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC110828 sequence for NM_000039 edited (data generated by NextGen Sequencing)
ATGAAAGCTGCGGTGCTGACCTTGGCCGTGCTCTTCCTGACGGGGAGCCAGGCTCGGCAT
TTCTGGCAGCAAGATGAACCCCCCCAGAGCCCCTGGGATCGAGTGAAGGACCTGGCCACT
GTGTACGTGGATGTGCTCAAAGACAGCGGCAGAGACTATGTGTCCCAGTTTGAAGGCTCC
GCCTTGGGAAAACAGCTAAACCTAAAGCTCCTTGACAACTGGGACAGCGTGACCTCCACC
TTCAGCAAGCTGCGCGAACAGCTCGGCCCTGTGACCCAGGAGTTCTGGGATAACCTGGAA
AAGGAGACAGAGGGCCTGAGGCAGGAGATGAGCAAGGATCTGGAGGAGGTGAAGGCCAAG
GTGCAGCCCTACCTGGACGACTTCCAGAAGAAGTGGCAGGAGGAGATGGAGCTCTACCGC
CAGAAGGTGGAGCCGCTGCGCGCAGAGCTCCAAGAGGGCGCGCGCCAGAAGCTGCACGAG
CTGCAAGAGAAGCTGAGCCCACTGGGCGAGGAGATGCGCGACCGCGCGCGCGCCCATGTG
GACGCGCTGCGCACGCATCTGGCCCCCTACAGCGACGAGCTGCGCCAGCGCTTGGCCGCG
CGCCTTGAGGCTCTCAAGGAGAACGGCGGCGCCAGACTGGCCGAGTACCACGCCAAGGCC
ACCGAGCATCTGAGCACGCTCAGCGAGAAGGCCAAGCCCGCGCTCGAGGACCTCCGCCAA
GGCCTGCTGCCCGTGCTGGAGAGCTTCAAGGTCAGCTTCCTGAGCGCTCTCGAGGAGTAC
ACTAAGAAGCTCAACACCCAGTGA

Clone variation with respect to NM_000039.1
>OriGene 5' read for NM_000039 unedited
GGATTTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGAGGAGGTCCCCCAC
GGCCCTTCAGGATGAAAGCTGCGGTGCTGACCTTGGCCGTGCTCTTCCTGACGGGGAGCC
AGGCTCGGCATTTCTGGCAGCAAGATGAACCCCCCCAGAGCCCCTGGGATCGAGTGAAGG
ACCTGGCCACTGTGTACGTGGATGTGCTCAAAGACAGCGGCAGAGACTATGTGTCCCAGT
TTGAAGGCTCCGCCTTGGGAAAACAGCTAAACCTAAAGCTCCTTGACAACTGGGACAGCG
TGACCTCCACCTTCAGCAAGCTGCGCGAACAGCTCGGCCCTGTGACCCAGGAGTTCTGGG
ATAACCTGGAAAAGGAGACAGAGGGCCTGAGGCAGGAGATGAGCAAGGATCTGGAGGAGG
TGAAGGCCAAGGTGCAGCCCTACCTGGACGACTTCCAGAAGAAGTGGCAGGAGGAGATGG
AGCTCTACCGCCAGAAGGTGGAGCCGCTGCGCGCAGAGCTCCAAGAGGGCGCGCGCCAGA
AGCTGCACGAGCTGCAAGAGAAGCTGAGCCCACTGGGCGAGGAGATGCGCGACCGCGCGC
GCGCCCATGTGGACGCGCTGCGCACGCATCTGGCCCCCTACAGCGACGAGCTGCGCCAGC
GCTTGGCCGCGCGCCTTGAGGCTCTCAAGGAGAACGGCGGCGCCAGACTGGCCGAGTACC
ACGCCAAGGCCACCGAGCATCTGAGCACGCTCAGCGAGAAGGCCAAGCCCGCGCTCGAGG
ACCTNCGCCAAGCCTGCTGCCCGTGCTGGAGAGCTTCAGGTCAGCTTCCTGAGCGCTCTC
GAGGGAGTACACTAGAGCTCACACCCAGTGAGGCGCCCNGCGCGCCCTTNNNCGTGCTAG
AATAAACGTTCCAAGTGGAAAAAAAAAAAAAAAAAAAAAACTCACTTAATGCGNCGGNCA
TACTGTTCTGACGACCGGGTGGATCTTGGACCTCCAGGCTTCGT
>OriGene 3' read for NM_000039 unedited
GCCATTGGNGATGGCACTNTCCAGGTCCAGNANAGCACTGGGGNAGGGTCACAGGGATGC
CACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCACTTTGGAAACGTTTATTCTGAGCACC
GGGAAGGGGGGCGGCGGCGGGCCCCTCACTGGGTGTTGAGCTTTTTAGTGTACTCCTCGA
GAGCGCTCAGGAAGCTGACCTTGAAGCTCTCCAGCACGGGCAGCAGGCCTTGGCGGAGGT
CCTCGAGCGCGGGCTTGGCCTTCTCGCTGAGCGTGCTCAAATGCTCGGTGGCCTTGGCGT
GGTACTCGGCCAGTCTGGCGCCGCCGTTCTCCTTGAGAGCCTCAAGGCGCGCGGCCAAGC
GCTGGCGCAGCTTGTCTCTGTAGGGGGCCAAATGCGTGCGCAGCGCGTCCACATGGGCGC
GCGCGCGGTCTCGCATCTCCTTTCCCAGTGGGCTCAGCTTCTTTTGCAGCTCGTGCAGTT
TCTGGCGCGCGCCCTCTTGGAGCTCTGCGCGCAGCGGCTCCATCTTTTGGCGGGAGAGCT
CCATCTCCTCCTGCCACTTTTTTTGGAAGTCTTCCAGGTAGGGTTGAACTTTGGCCTTCA
CCCCCTCCAAATTCTTGGTCATCTCCTGCCCTAGGCCCTCTGTCTTCTTTTTCAGGTTAT
CCCAAAACTTCTGGGTCACAAGGGCCGAACTTGTTCCCGCAACTTGTTGAAAGGGGGAGG
TCACCCTCTTCCATTTTGCAAAGGAGCCTTTAAGTTTAACTTGTTTCCCAAGGCGGGGCC
CTTAAACTGGGAAAAAATTCCTCTGCCCTGTTTTTTACCCATCCACTTCCGGTGGCCAGT
TTCTTCACATCATCCCGGGGTTTTG
Restriction Sites NotI-NotI     
ACCN NM_000039
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000039.1, NP_000030.1
RefSeq Size 897 bp
RefSeq ORF 804 bp
Locus ID 335
Cytogenetics 11q23.3
Domains Apolipoprotein
Protein Families Druggable Genome, Secreted Protein
Protein Pathways PPAR signaling pathway
Gene Summary 'This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform. Variants 1-3 encode the same isoform (1).

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