ASS1 (NM_000050) Human Untagged Clone

CAT#: SC110830

ASS1 (untagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASS1
• Synonyms: ASS; CTLN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110830 sequence for NM_000050 edited (data generated by NextGen Sequencing)
  ATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGACACCTCGTGCATC
  CTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGCCAACATTGGCCAG
  AAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGGGGCCAAAAAGGTGTTC
  ATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCCGGCCATCCAGTCCAGC
  GCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAGGCCCTGCATCGCCCGC
  AAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCACGGCGCCACAGGA
  AAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACTGGCCCCCCAGATAAAG
  GTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAAGGGCCGCAATGACCTG
  ATGGAGTACGCAAAGCAACACGGGATTCCCATCCCGGTCACTCCCAAGAACCCGTGGAGC
  ATGGATGAGAACCTCATGCACATCAGCTACGAGGCTGGAATCCTGGAGAACCCCAAGAAC
  CAAGCGCCTCCAGGTCTCTACACGAAGACCCAGGACCCAGCCAAAGCCCCCAACACCCCT
  GACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGAAGGTGACCAACGTCAAGGAT
  GGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAACGAAGTCGCGGGCAAG
  CATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT
  ATCTACGAGACCCCAGCAGGCACCATCCTTTACCATGCTCATTTAGACATCGAGGCCTTC
  ACCATGGACCGGGAAGTGCGCAAAATCAAACAAGGCCTGGGCTTGAAATTTGCTGAGCTG
  GTGTATACCGGTTTCTGGCACAGCCCTGAGTGTGAATTTGTCCGCCACTGCATCGCCAAG
  TCCCAGGAGCGAGTGGAAGGGAAAGTGCAGGTGTCCGTCCTCAAGGGCCAGGTGTACATC
  CTCGGCCGGGAGTCCCCACTGTCTCTCTACAATGAGGAGCTGGTGAGCATGAACGTGCAG
  GGTGATTATGAGCCAACTGATGCCACCGGGTTCATCAACATCAATTCCCTCAGGCTGAAG
  GAATATCATCGTCTCCAGAGCAAGGTCACTGCCAAATAG
  
  Clone variation with respect to NM_000050.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000050 unedited
  GATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCCACCGCTGCC
  CGAGCCCGACGCTATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGA
  CACCTCGTGCATCCTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGC
  CAACATTGGCCAGAAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGGGGC
  CAAAAAGGTGTTCATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCCGGC
  CATCCAGTCCAGCGCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAGGCC
  CTGCATCGCCCGCAAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCA
  CGGCGCCACAGGAAAGGGGAACGATCAGGTCCCGGTTTGAGCTCAGCTGCTACTCACTGG
  CCCCCCAGATAAAGGTCATTGCTCNTTGGAGGATGCCTGAATTCTACAACCGGTTCAAGG
  GCCGCAATGACCTGATGGAGTACGCAAAGCAACACGGGATTCCAATCCCGGTCACTCCCA
  AGACCCGTGGAGATGGGNATGAGAACCTCATGCACATCAGCTACGAAGCTGGGAATCCTG
  GAGAACCCCAAGAACCAAGCGCCTNNCAGGTCTCTACACCGAAGACCCAGGACCCAGCCA
  AAGCCCCCAACACCCCTGAATTNTTCGAGAAAACGAGTTCAAANAAAGGGGGTCTGTGGG
  AAAGGTGAACAACGTCAAAGGAATGGGACCACCCACCAGACCTCCTTGGAGCTCCTCATG
  TACCTGAACCAAANTCGCGGCAGCATGGGCGGGGGGCCGAATGCAATTCGGGGGAGACCC
  GCTCATTGGAAAGAACCCCCCGAGGTTTTAAAAAACCCCCACAGCCACAATCCTTCCATG
  CTCATTAAAAAACGAGGCCCTACATGACCGCGGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000050 unedited
  NGGTTTTTACTNATGNANCGCGGNNCCGCATTTNANGAATCGATTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGGGCATTTTTAAAGCTCCCCACC
  GAAGTTGCCCCCCACCCTTCCTTTTAATAACAACGTTTGCAGGCTTAAGGGGGACCAGGG
  AACAAAGCTGGGGCCTGGAAGCCCCATTACGCTGCCAGCCGGGGAAAACAAGTCACAATT
  ACAAATTATCACAACAATTACCGCCTGTACTTGGGGGATCTGCAAATTGAGGAGGCCCCA
  CCTCCTTATTGTACACGGGTTTATTTGGCAGGGACCTTGCTTTGGAAACAAAAATTTTCC
  TTCACCCTGAGGGAATTGATGTTGATAAACCCGGGGGCATAAGTTGGCTAAAAATAACCC
  TGCACGTTAATGCTCACCAACTCCTTATTGTAAAAAAACAGGGGGGACTCCCGGCCGAGG
  ATGTACACCTGGCCCTTGAGGACGGACACCTGCACTTTCCCTTCCACTCGCTCCTGGGAC
  TTGGCAATGCAGGGGCGGACAAATTCACACTCAGGGCTGGGCCAAAAACCGGTATACACC
  AGCTCAAAAAATTTCAAGCCCAGGCCTTGTTTGATTTTGCGCACTTCCCGGCCCATGGGG
  AAGGCCTCAATGTCTAAATGACCATGGAAAAGGATGGGGCCTGCTGGGGTCTCGAAAAAC
  CTCGGGACTAATTCCAATGAAGCGGTTCTCCACAATGTAAATACGGCCCACGCCATGCTT
  GCCCGGAACTTNGTTAAGGTACATGAAAAGCTCCAAGGAGGTCTGGGGGGGTGGGGGCAT
  CCTTGACGTTGGACACCCTCACAGGACCCCTTTTTTGAACTCGATCTCAAATGTCAAGGG
  GTGTTGGGGCTTTGGCTGGGCCCTGGGGCCTTCGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000050
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000050.3, NP_000041.2
• RefSeq Size: 1582 bp
• RefSeq ORF: 1239 bp
• Locus ID: 445
• Cytogenetics: 9q34.11
• Domains: Arginosuc_synth
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012]'
  Transcript Variant: This variant (1) represents the longer variant.