ASS1 (NM_000050) Human Untagged Clone

CAT#: SC110830

ASS1 (untagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 1


  "NM_000050" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ASS1
Synonyms ASS; CTLN1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC110830 sequence for NM_000050 edited (data generated by NextGen Sequencing)
ATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGACACCTCGTGCATC
CTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGCCAACATTGGCCAG
AAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGGGGCCAAAAAGGTGTTC
ATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCCGGCCATCCAGTCCAGC
GCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAGGCCCTGCATCGCCCGC
AAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCACGGCGCCACAGGA
AAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACTGGCCCCCCAGATAAAG
GTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAAGGGCCGCAATGACCTG
ATGGAGTACGCAAAGCAACACGGGATTCCCATCCCGGTCACTCCCAAGAACCCGTGGAGC
ATGGATGAGAACCTCATGCACATCAGCTACGAGGCTGGAATCCTGGAGAACCCCAAGAAC
CAAGCGCCTCCAGGTCTCTACACGAAGACCCAGGACCCAGCCAAAGCCCCCAACACCCCT
GACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGAAGGTGACCAACGTCAAGGAT
GGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAACGAAGTCGCGGGCAAG
CATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT
ATCTACGAGACCCCAGCAGGCACCATCCTTTACCATGCTCATTTAGACATCGAGGCCTTC
ACCATGGACCGGGAAGTGCGCAAAATCAAACAAGGCCTGGGCTTGAAATTTGCTGAGCTG
GTGTATACCGGTTTCTGGCACAGCCCTGAGTGTGAATTTGTCCGCCACTGCATCGCCAAG
TCCCAGGAGCGAGTGGAAGGGAAAGTGCAGGTGTCCGTCCTCAAGGGCCAGGTGTACATC
CTCGGCCGGGAGTCCCCACTGTCTCTCTACAATGAGGAGCTGGTGAGCATGAACGTGCAG
GGTGATTATGAGCCAACTGATGCCACCGGGTTCATCAACATCAATTCCCTCAGGCTGAAG
GAATATCATCGTCTCCAGAGCAAGGTCACTGCCAAATAG

Clone variation with respect to NM_000050.4
>OriGene 5' read for NM_000050 unedited
GATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCCACCGCTGCC
CGAGCCCGACGCTATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGA
CACCTCGTGCATCCTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGC
CAACATTGGCCAGAAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGGGGC
CAAAAAGGTGTTCATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCCGGC
CATCCAGTCCAGCGCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAGGCC
CTGCATCGCCCGCAAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCA
CGGCGCCACAGGAAAGGGGAACGATCAGGTCCCGGTTTGAGCTCAGCTGCTACTCACTGG
CCCCCCAGATAAAGGTCATTGCTCNTTGGAGGATGCCTGAATTCTACAACCGGTTCAAGG
GCCGCAATGACCTGATGGAGTACGCAAAGCAACACGGGATTCCAATCCCGGTCACTCCCA
AGACCCGTGGAGATGGGNATGAGAACCTCATGCACATCAGCTACGAAGCTGGGAATCCTG
GAGAACCCCAAGAACCAAGCGCCTNNCAGGTCTCTACACCGAAGACCCAGGACCCAGCCA
AAGCCCCCAACACCCCTGAATTNTTCGAGAAAACGAGTTCAAANAAAGGGGGTCTGTGGG
AAAGGTGAACAACGTCAAAGGAATGGGACCACCCACCAGACCTCCTTGGAGCTCCTCATG
TACCTGAACCAAANTCGCGGCAGCATGGGCGGGGGGCCGAATGCAATTCGGGGGAGACCC
GCTCATTGGAAAGAACCCCCCGAGGTTTTAAAAAACCCCCACAGCCACAATCCTTCCATG
CTCATTAAAAAACGAGGCCCTACATGACCGCGGAAA
>OriGene 3' read for NM_000050 unedited
NGGTTTTTACTNATGNANCGCGGNNCCGCATTTNANGAATCGATTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGGGCATTTTTAAAGCTCCCCACC
GAAGTTGCCCCCCACCCTTCCTTTTAATAACAACGTTTGCAGGCTTAAGGGGGACCAGGG
AACAAAGCTGGGGCCTGGAAGCCCCATTACGCTGCCAGCCGGGGAAAACAAGTCACAATT
ACAAATTATCACAACAATTACCGCCTGTACTTGGGGGATCTGCAAATTGAGGAGGCCCCA
CCTCCTTATTGTACACGGGTTTATTTGGCAGGGACCTTGCTTTGGAAACAAAAATTTTCC
TTCACCCTGAGGGAATTGATGTTGATAAACCCGGGGGCATAAGTTGGCTAAAAATAACCC
TGCACGTTAATGCTCACCAACTCCTTATTGTAAAAAAACAGGGGGGACTCCCGGCCGAGG
ATGTACACCTGGCCCTTGAGGACGGACACCTGCACTTTCCCTTCCACTCGCTCCTGGGAC
TTGGCAATGCAGGGGCGGACAAATTCACACTCAGGGCTGGGCCAAAAACCGGTATACACC
AGCTCAAAAAATTTCAAGCCCAGGCCTTGTTTGATTTTGCGCACTTCCCGGCCCATGGGG
AAGGCCTCAATGTCTAAATGACCATGGAAAAGGATGGGGCCTGCTGGGGTCTCGAAAAAC
CTCGGGACTAATTCCAATGAAGCGGTTCTCCACAATGTAAATACGGCCCACGCCATGCTT
GCCCGGAACTTNGTTAAGGTACATGAAAAGCTCCAAGGAGGTCTGGGGGGGTGGGGGCAT
CCTTGACGTTGGACACCCTCACAGGACCCCTTTTTTGAACTCGATCTCAAATGTCAAGGG
GTGTTGGGGCTTTGGCTGGGCCCTGGGGCCTTCGGC
Restriction Sites NotI-NotI     
ACCN NM_000050
Insert Size 1600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000050.3, NP_000041.2
RefSeq Size 1582 bp
RefSeq ORF 1239 bp
Locus ID 445
Cytogenetics 9q34.11
Domains Arginosuc_synth
Protein Families Druggable Genome
Protein Pathways Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways
Gene Summary 'The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012]'
Transcript Variant: This variant (1) represents the longer variant.

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