Choline kinase alpha (CHKA) (NM_001277) Human Untagged Clone

CAT#: SC110854

CHKA (untagged)-Human choline kinase alpha (CHKA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHKA
• Synonyms: CHK; CK; CKI; EK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001277, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAAACCAAATTCTGCACCGGGGGCGAGGCGGAGCCCTCGCCGCTCGGGCTGCTGCTGAGCTGCGGTA
  GCGGCAGCGCGGCCCCGGCGCCCGGCGTGGGGCAGCAGCGCGACGCCGCCAGCGACCTCGAGTCCAAGCA
  GCTGGGCGGCCAACAGCCGCCGCTCGCGCTGCCCCCTCCGCCGCCGCTGCCGCTGCCGCTGCCGCTGCCC
  CAGCCCCCGCCGCCGCAGCCGCCCGCAGACGAGCAGCCGGAGCCCCGGACGCGGCGCAGGGCCTATCTGT
  GGTGCAAGGAGTTCCTGCCCGGCGCCTGGCGGGGCCTCCGCGAGGACGAGTTCCACATCAGTGTCATCAG
  AGGCGGCCTTAGCAACATGCTGTTCCAGTGCTCCCTACCTGACACCACAGCCACCCTTGGTGATGAGCCT
  CGGAAAGTGCTCCTGCGGCTGTATGGAGCGATTTTGCAGATGAGGTCCTGTAATAAAGAGGGATCCGAAC
  AAGCTCAGAAAGAAAATGAATTTCAAGGGGCTGAGGCCATGGTTCTGGAGAGCGTTATGTTTGCCATTCT
  CGCAGAGAGGTCACTTGGGCCAAAACTCTATGGCATCTTTCCCCAAGGCCGACTGGAGCAGTTCATCCCG
  AGCCGGCGATTAGATACTGAAGAATTAAGTTTGCCAGATATTTCTGCAGAAATCGCCGAGAAAATGGCTA
  CATTTCATGGTATGAAAATGCCATTCAATAAGGAACCAAAATGGCTTTTTGGCACAATGGAAAAGTATCT
  AAAGGAAGTGCTGAGAATTAAATTTACTGAGGAATCCAGAATTAAAAAGCTCCACAAATTGCTCAGTTAC
  AATCTGCCCTTGGAACTGGAAAACCTGAGATCATTGCTTGAATCTACTCCATCTCCAGTTGTATTTTGTC
  ATAATGACTGTCAAGAAGGTAATATCTTGTTGCTGGAAGGCCGAGAGAATTCTGAAAAACAGAAACTGAT
  GCTCATTGATTTCGAATACAGCAGTTACAATTACAGGGGATTCGACATTGGAAATCACTTCTGTGAGTGG
  ATGTATGATTATAGCTATGAAAAATACCCTTTTTTCAGAGCAAACATCCGGAAGTATCCCACCAAGAAAC
  AACAGCTCCATTTTATTTCCAGTTACTTGCCTGCATTCCAAAATGACTTTGAAAACCTCAGTACTGAAGA
  AAAATCCATTATAAAAGAAGAAATGTTGCTTGAAGTTAATAGGTTTGCCCTTGCATCTCATTTCCTCTGG
  GGACTGTGGTCCATTGTACAAGCCAAGATTTCATCTATTGAATTTGGGTACATGGACTACGCCCAAGCAA
  GGTTTGATGCCTATTTCCACCAGAAGAGGAAGCTTGGGGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001277 unedited
  GCACGAGGGCGCCCGGCCGGACTCTGAGCCTAGTCCTCTCGCGCTGCGGCCGCCCGCGCC
  TCCTCGGCCGCCTGTCGGGCATGAAAACCAAATTCTGCACCGGGGGCGAGGCGGAGCCCT
  CGCCGCTCGGGCTGCTGCTGAGCTGCGGTAGCGGCAGCGCGGCCCCGGCGCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001277 unedited
  CTCGAGGCCCGCAATTTAGNGTCGAGTTTTTTTCTTTTTTTTTTGGGTTTTTATTTTTTT
  ATTTGTATAGTACAATCTCTGTGGTAGCAGAAATTATGCACAAAAAACAATTCAAATAAG
  AGTTCAGAGTAAAAAAAAGTCAGCTAAGACTGTATCCCGCAGGACATTTCATATATATGG
  ATTTCACAGCTACACTCGATCCACGCCCCACACCACATACAGGCTGCTGTCTCCCACAAG
  AATGATGATGTCAGGGGCATCAGGAAAGGTAAGGGCCGGGAAACCGGGCCCTTGGAGAAC
  CCTGCCCAGGGGAGGCCCAGCCTACTCACAGGATCCGACACTCCAGGCAGAGCAGAGGGC
  AGGAGAGGCCCAAAGAGCTAGGTCAAGCAGCTGGCTCCCCTGGGAGGGGAGCCGGGAGAC
  AGGGAGGTCCTCACACTGCCCCCGTGGCCACGCATGGCTGTCTGCACACTCCATCAGGAG
  GAAGGGCAGGGGAGAAGTCACATGCAGTACAGTGAAACTCATCAGTGTCACACACGCCCA
  GCAGGAGGGGAGAGCTCTGCTCCACACGCTGGCGGCCTTGGCTGCTCCAGAGCAATCCGC
  TGCTCCAGCTTCAGCCATGGGACCAAGAGGGTAAAGGAGCAGCTTCTGGAGGCCCCAGGC
  AGAGGCCACCTTGCCACAGTGACTGGCGACTNTGCAGTACATCTCTGCTCACTTGCTGTC
  CCTTGGGAGTGAGCACCGTCTATAACACATCGGCATCTACAAAACCTGGTACAGTGTTAC
  CCAGCCATCATGAAAATTAAATCAACATTACCAAGTCCCTGCAGGTACCCTGCTCACTCT
  TGCTGGGTGCCTCATCTGACTGGAACACTTAACCCCCAATATGAAATGCCCTCTCTAGAA
  TAAAAGATACAAAAGTGTGCCCTTTGCACTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001277
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277.2, NP_001268.2
• RefSeq Size: 2733 bp
• RefSeq ORF: 1374 bp
• Locus ID: 1119
• Cytogenetics: 11q13.2
• Domains: Choline_kinase
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: 'The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).