CHRM2 (NM_000739) Human Untagged Clone

CAT#: SC110855

CHRM2 (untagged)-Human cholinergic receptor, muscarinic 2 (CHRM2), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRM2
• Synonyms: HM2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110855 sequence for NM_000739 edited (data generated by NextGen Sequencing)
  ATGAATAACTCAACAAACTCCTCTAACAATAGCCTGGCTCTTACAAGTCCTTATAAGACA
  TTTGAAGTGGTGTTTATTGTCCTGGTGGCTGGATCCCTCAGTTTGGTGACCATTATCGGG
  AACATCCTAGTCATGGTTTCCATTAAAGTCAACCGCCACCTCCAGACCGTCAACAATTAC
  TTTTTATTCAGCTTGGCCTGTGCTGACCTTATCATAGGTGTTTTCTCCATGAACTTGTAC
  ACCCTCTACACTGTGATTGGTTACTGGCCTTTGGGACCTGTGGTGTGTGACCTTTGGCTA
  GCCCTGGACTATGTGGTCAGCAATGCCTCAGTTATGAATCTGCTCATCATCAGCTTTGAC
  AGGTACTTCTGTGTCACAAAACCTCTGACCTACCCAGTCAAGCGGACCACAAAAATGGCA
  GGTATGATGATTGCAGCTGCCTGGGTCCTCTCTTTCATCCTCTGGGCTCCAGCCATTCTC
  TTCTGGCAGTTCATTGTAGGGGTGAGAACTGTGGAGGATGGGGAGTGCTACATTCAGTTT
  TTTTCCAATGCTGCTGTCACCTTTGGTACGGCTATTGCAGCCTTCTATTTGCCAGTGATC
  ATCATGACTGTGCTATATTGGCACATATCCCGAGCCAGCAAGAGCAGGATAAAGAAGGAC
  AAGAAGGAGCCTGTTGCCAACCAAGACCCCGTTTCTCCAAGTCTGGTACAAGGAAGGATA
  GTGAAGCCAAACAATAACAACATGCCCAGCAGTGACGATGGCCTGGAGCACAACAAAATC
  CAGAATGGCAAAGCCCCCAGGGATCCTGTGACTGAAAACTGTGTTCAGGGAGAGGAGAAG
  GAGAGCTCCAATGACTCCACCTCAGTCAGTGCTGTTGCCTCTAATATGAGAGATGATGAA
  ATAACCCAGGATGAAAACACAGTTTCCACTTCCCTGGGCCATTCCAAAGATGAGAACTCT
  AAGCAAACATGCATCAGAATTGGCACCAAGACCCCAAAAAGTGACTCATGTACCCCAACT
  AATACCACCGTGGAGGTAGTGGGGTCTTCAGGTCAGAATGGAGATGAAAAGCAGAATATT
  GTAGCCCGCAAGATTGTGAAGATGACTAAGCAGCCTGCAAAAAAGAAGCCTCCTCCTTCC
  CGGGAAAAGAAAGTCACCAGGACAATCTTGGCTATTCTGTTGGCTTTCATCATCACTTGG
  GCCCCATACAATGTCATGGTGCTCATTAACACCTTTTGTGCACCTTGCATCCCCAACACT
  GTGTGGACAATTGGTTACTGGCTTTGTTACATCAACAGCACTATCAACCCTGCCTGCTAT
  GCACTTTGCAATGCCACCTTCAAGAAGACCTTTAAACACCTTCTCATGTGTCATTATAAG
  AACATAGGCGCTACAAGGTAA
  
  Clone variation with respect to NM_000739.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000739 unedited
  ATAACCCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGTATTTTGGGCTTCCCGCTCTTTGTGAAAGCCCGATCAAGGGAG
  AAAGAGAACCGGCAGCTGGGCTCGGACTCTAAGCGGGGCGCAACTTCAGCCCGAGCGGAT
  CGGGCCTTAACCCACAAAGGACTCCTCGCTTCTTTAAGCCTTCACCACCTTGCAGGCGGG
  GAGGGAACTGGGGCGAAACCCGCGACAGGGTTAAATGGTTATTTGCTACTCGGCTACTGG
  GTAGAGAACGCCAAATGAATAACTCAACAAACTCCTCTAACAATAACCTGGGTCTTACAA
  GACCTTATAATACATTTGAAGTGGGGTTTATTGGCCTGGTGGGTGGATCCCTCACTTTGG
  TGACCATTATCGGGGACATCCTACTCATGGGTTCCATTAAAGTCAACCGCCACCTCCAGA
  CCGTGAACAATTACTTTTTATTCAGCTTGGCCTGTGCTGACCTTATCATAAGAGAGGTGT
  CCATGAACTTGTACACCCTCTACACTGTGATTGGGTACTGGCCTTTGTGACCTGTGGTGT
  GTGACCTTTGGCTAGCCCTGCACTATGTGGTCAGCAATGCCTCACTTATGAATCTGCTCA
  TCATCAACTNTGACAGGAACTTCTGTGTGACAGAACCTCTGACCTACCCAGTCAAGCGGA
  CCACACAAATGGCAGGNATGATGATTGCAGCTGCCTGGGTCCTCTCTTTCATTCTCTGGG
  CTGCCGCCATTCTCTTCTGGCAGTTATTGTATGGGTGAGAACTGACGAGATGGGGAGTGC
  TACATTCANATCTTTCCATGCTGCTGCCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000739 unedited
  AGGTAGCATATAGNCNCGCGGGCCGCTATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTGT
  GTATAATATATTTATTTGCATACAGGAAAATGTATTTCTATTGTACATTTTATTATTTTA
  TTTATAGAATATATATATATATTTAGCACATAGCATATTCCAGGAAGATACATTAAATAT
  GCACACATTTCATGCTACAGCGTGCACCCTTTAAGAGAAGCATTTTTAAGAGGCATGGTA
  TTGAATATACATGCTGAGAATACACTTTGCACAATTTCATCCAGCTTTGGGTGCTTTCCT
  AAACTATTTGCAACACCGGTACTATGGTTGTTGAAAGGGATATTATCAAACTGGGGCGAG
  GAGGAAACTGTCAATGAACTAAGTAGGAGAAAATAAAAACAGACTGCATTGCTGAGTAAT
  TGGTGCCATCAATTTGAATTCCCATCTCTCCCAATGTCAGATCTTCAGGATCAAAAAAGC
  AAATGAACAAAGAGCAGGAGTATGTTGGACATCCAGCTTGTTCATTTCAAATCAGAAAGC
  TTACCCGCTGTGGCTTCTCATATTGATTACTCTGCCCTAATACTTGGGAGCTGGCGTTAA
  ATTAATGATGAACTGGTAAAAGGAAAACATTTCTTGTTTCGAAGACATGAACAGACTTTT
  TCCATATTCTTAATCTCCTCTTCTTATAATATCACTCTGGGATAGCTACATTATTGCTAT
  GAGGAGAGAAAAAACAAGACCTTACAGATACCCTTTTCAGTAATGCAAAGAAATAAATGA
  TATGTTTCTGAAGAGGCTCTATATTTTTTGAAGGAAAATTGAGCTCCGTAGANCTGATCT
  ATACATGCAATATTTGATGGCAATACTGCAACTCAATGACTAATTCGTGGGATTCTATCT
  AACCTAAGAGCTGGAAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000739
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000739.2, NP_000730.1
• RefSeq Size: 2530 bp
• RefSeq ORF: 1401 bp
• Locus ID: 1129
• Cytogenetics: 7q33
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Gene Summary: 'The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 through 8 encode the same protein (isoform a).