CRF1 (CRHR1) (NM_004382) Human Untagged Clone

CAT#: SC110860

CRHR1 (untagged)-Human corticotropin releasing hormone receptor 1 (CRHR1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRHR1
• Synonyms: CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRHR; CRHR1L
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110860 sequence for NM_004382 edited (data generated by NextGen Sequencing)
  ATGGGAGGGCACCCGCAGCTCCGTCTCGTCAAGGCCCTTCTCCTTCTGGGGCTGAACCCC
  GTCTCTGCCTCCCTCCAGGACCAGCACTGCGAGAGCCTGTCCCTGGCCAGCAACATCTCA
  GGACTGCAGTGCAACGCATCCGTGGACCTCATTGGCACCTGCTGGCCCCGCAGCCCTGCG
  GGGCAGCTAGTGGTTCGGCCCTGCCCTGCCTTTTTCTATGGTGTCCGCTACAATACCACA
  AACAATGGCTACCGGGAGTGCCTGGCCAATGGCAGCTGGGCCGCCCGCGTGAATTACTCC
  GAGTGCCAGGAGATCCTCAATGAGGAGAAAAAAAGCAAGGTGCACTACCATGTCGCAGTC
  ATCATCAACTACCTGGGCCACTGTATCTCCCTGGTGGCCCTCCTGGTGGCCTTTGTCCTC
  TTTCTGCGGCTCAGGAGCATCCGGTGCCTGCGAAACATCATCCACTGGAACCTCATCTCC
  GCCTTCATCCTGCGCAACGCCACCTGGTTCGTGGTCCAGCTAACCATGAGCCCCGAGGTC
  CACCAGAGCAACGTGGGCTGGTGCAGGTTGGTGACAGCCGCCTACAACTACTTCCATGTG
  ACCAACTTCTTCTGGATGTTCGGCGAGGGCTGCTACCTGCACACAGCCATCGTGCTCACC
  TACTCCACTGACCGGCTGCGCAAATGGATGTTCATCTGCATTGGCTGGGGTGTGCCCTTC
  CCCATCATTGTGGCCTGGGCCATTGGGAAGCTGTACTACGACAATGAGAAGTGCTGGTTT
  GGCAAAAGGCCTGGGGTGTACACCGACTACATCTACCAGGGCCCCATGATCCTGGTCCTG
  CTGATCAATTTCATCTTCCTTTTCAACATCGTCCGCATCCTCATGACCAAGCTCCGGGCA
  TCCACCACGTCTGAGACCATTCAGTACAGGAAGGCTGTGAAAGCCACTCTGGTGCTGCTG
  CCCCTCCTGGGCATCACCTACATGCTGTTCTTCGTCAANCCCGGGGAGGATGAGGTCTCC
  CGGGTCGTCTTCATCTACTTCAACTCCTTCCTGGAATCCTTCCAGGGCTTCTTTGTGTCT
  GTGTTCTACTGTTTCCTCAATAGTGAGGTCCGTTCTGCCATCCGGAAGAGGTGGCACCGG
  TGGCAGGACAAGCACTCGATCCGTGCCCGAGTGGCCCGTGCCATGTCCATCCCCACCTCC
  CCAACCCGTGTCAGCTTTCACAGCATCAAGCAGTCCACAGCAGTCTGA
  
  Clone variation with respect to NM_004382.4
  999 t=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004382 unedited
  NGGTCAAAATTTGTATACGACTCATATAGGCGGCNCGCGATTCGGCACGAGGGAGAGCCG
  GGCCGGGCCGGGCCGGGCCGCGGGGCCGGGAAGCGCCGAGCCGGGCATCTCCTCACCAGG
  CAGCGACCGAGGAGCCCGGCCGCCCACCCCGTGCCGCCCGAGCCCGCAGCCGCCCGCCGG
  TCCCTCTGGGATGTCCGTAGGACCCGGGCATTCAGGACGGTAGCCGAGCGAGCCCGAGGA
  TGGGAGGGCACCCGCAGCTCCGTCTCGTCAAGGCCCTTCTCCTTCTGGGGCTGAACCCCG
  TCTCTGCCTCCCTCCAGGACCAGCACTGCGAGAGCCTGTCCCTGGCCAGCAACATCTCAG
  GACTGCAGTGCAACGCATCCGTGGACCTCATTGGCACCTGCTGGCCCCGCAGCCCTGCGG
  GGCAGCTAGTGGTTCGGCCCTGCCCTGCCTTTTTCTATGGTGTCCGCTACAATACCACAA
  ACAATGGCTACCGGGAGTGCCTGGCCAATGGCAGCTGGGCCGCCCGCGTGAATTACTCCG
  AGTGCCAGGAGATCCTCAATGAGGAGAAAAAAAGCAAGGTGCACTACCATGTCGCAGTCA
  TCATCAACTACCTGGGCCACTGTATCTCCCTGGTGGCCCTCCTGGTGGCCTTTGTCCTCT
  TTCTGCGGCTCAGGAGCATCCGGTGCCTGCGAAACATCATCCACTGGAACCTCATCTCCG
  CCTTCATCCTGCGCAACGCCACCTGGTTTCGTGGTCCAGCTAACCATGACCCCCCGAGGT
  CCACCAGACCAACGTGGGCTGGTGGCCAGGGTTGGNTGACAGCCGCCTACAACTACTTNC
  ATGTGACCAACTCTNTTCTGGATGTTCNGGNGAAGGGCTGCTACCTGCACACANNCATCG
  TGCTCACCTACTNCACTGACCGCTGCGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004382 unedited
  CCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTGGTTGAAAAGAAAA
  TTTTATTTATACCAGCCACCCGCCTCACAGCCGACACCCTCATCTTCTAGTGCCCCCCAA
  AGCCCTGCCCTGGCTGTCCAGTCCCTCTGGACATGGGCAGGTCAGTGGGGGCTGCGGCCG
  GTCCACACCTGGAGTGTAAGCAGCACGTTGTCCCAAGAGCCACTTGGGCAGGGGTCTTCT
  CCTGGCTTGCTTAGCTAGTGGTCCTGCCCCAGAGGCCATCCAGGGCTACAAGCTCTGCCC
  CAGAGGCTGGGACTGGGACACCCCTGGCTCTTGCTCACAGGCCACTCTGCCCCCTCCAGC
  CCCCATCTTCTCACAAAAGAGGAAAAGGAGCAGGAGGTGACTGGTATGGGGTGGTTAAGT
  GAGGGGAAGCTGGCCTGGCCTGCAGGGTACTAAATGTTCAGGGTGAAGGCAGCAAGGCAG
  GGCATTGCTGGTGGCAGTGCCACAGTGCCAGTAAGGTTCTGGAGGCCTGGGGGGGTGACT
  CTAGTGCTGTGGCCGCAAGTCTGATGATGACACCTGACTTCTGTCTCCAGGGTTCCTGAG
  TGAGGGCCCTTGGTTCCCAGTGGTGTCGGAAGGCATCACCGANGTCCAGAGGCGTCATCG
  TGGTGAGTCAGAGGCTGTCACGAGTTGCCCATGATGNCCCAGGGCAGCAAATGGCCTCCC
  CACGGTTGCCGNAGGCAGCCCCANGGNCCANNTGGGCTGNCCTTCTTGTGCTCTGGGAGA
  GACAGCCTTNGAGGACATGCGTGCTGCTGTAAGTGTCCAGCGCCCCATTTCATNCATTCC
  ATGTCCCTTCTCAGGAGGATTGGCAAGGAACCNAGCCCTGGCCCGNCCANCCTGTAGGGA
  CTTCACTCTGAACTNAGTCTCGCCGGTGCAGGGAAAGGGGCCCCCAGCTGNNATGCATTG
  TCTGGGGAGGGGCTGAACCTACAGCACTCCACGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004382
• Insert Size: 2740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004382.2, NP_004373.2
• RefSeq Size: 2536 bp
• RefSeq ORF: 1248 bp
• Locus ID: 1394
• Cytogenetics: 17q21.31
• Domains: 7tm_2, HormR
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Long-term depression, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Aug 2016]'
  Transcript Variant: This variant (1a, also known as CRHR1alpha), lacks an alternate in-frame exon in the central coding region, compared to variant 1b, resulting in an isoform (2) that is shorter than isoform 1.