CRF1 (CRHR1) (NM_004382) Human Tagged ORF Clone

CAT#: RC216124

CRHR1 (Myc-DDK-tagged)-Human corticotropin releasing hormone receptor 1 (CRHR1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY418036) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216124 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRHR1
• Synonyms: CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRHR; CRHR1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216124 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGGCACCCGCAGCTCCGTCTCGTCAAGGCCCTTCTCCTTCTGGGGCTGAACCCCGTCTCTGCCT
  CCCTCCAGGACCAGCACTGCGAGAGCCTGTCCCTGGCCAGCAACATCTCAGGACTGCAGTGCAACGCATC
  CGTGGACCTCATTGGCACCTGCTGGCCCCGCAGCCCTGCGGGGCAGCTAGTGGTTCGGCCCTGCCCTGCC
  TTTTTCTATGGTGTCCGCTACAATACCACAAACAATGGCTACCGGGAGTGCCTGGCCAATGGCAGCTGGG
  CCGCCCGCGTGAATTACTCCGAGTGCCAGGAGATCCTCAATGAGGAGAAAAAAAGCAAGGTGCACTACCA
  TGTCGCAGTCATCATCAACTACCTGGGCCACTGTATCTCCCTGGTGGCCCTCCTGGTGGCCTTTGTCCTC
  TTTCTGCGGCTCAGGAGCATCCGGTGCCTGCGAAACATCATCCACTGGAACCTCATCTCCGCCTTCATCC
  TGCGCAACGCCACCTGGTTCGTGGTCCAGCTAACCATGAGCCCCGAGGTCCACCAGAGCAACGTGGGCTG
  GTGCAGGTTGGTGACAGCCGCCTACAACTACTTCCATGTGACCAACTTCTTCTGGATGTTCGGCGAGGGC
  TGCTACCTGCACACAGCCATCGTGCTCACCTACTCCACTGACCGGCTGCGCAAATGGATGTTCATCTGCA
  TTGGCTGGGGTGTGCCCTTCCCCATCATTGTGGCCTGGGCCATTGGGAAGCTGTACTACGACAATGAGAA
  GTGCTGGTTTGGCAAAAGGCCTGGGGTGTACACCGACTACATCTACCAGGGCCCCATGATCCTGGTCCTG
  CTGATCAATTTCATCTTCCTTTTCAACATCGTCCGCATCCTCATGACCAAGCTCCGGGCATCCACCACGT
  CTGAGACCATTCAGTACAGGAAGGCTGTGAAAGCCACTCTGGTGCTGCTGCCCCTCCTGGGCATCACCTA
  CATGCTGTTCTTCGTCAATCCCGGGGAGGATGAGGTCTCCCGGGTCGTCTTCATCTACTTCAACTCCTTC
  CTGGAATCCTTCCAGGGCTTCTTTGTGTCTGTGTTCTACTGTTTCCTCAATAGTGAGGTCCGTTCTGCCA
  TCCGGAAGAGGTGGCACCGGTGGCAGGACAAGCACTCGATCCGTGCCCGAGTGGCCCGTGCCATGTCCAT
  CCCCACCTCCCCAACCCGTGTCAGCTTTCACAGCATCAAGCAGTCCACAGCAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216124 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGGHPQLRLVKALLLLGLNPVSASLQDQHCESLSLASNISGLQCNASVDLIGTCWPRSPAGQLVVRPCPA
  FFYGVRYNTTNNGYRECLANGSWAARVNYSECQEILNEEKKSKVHYHVAVIINYLGHCISLVALLVAFVL
  FLRLRSIRCLRNIIHWNLISAFILRNATWFVVQLTMSPEVHQSNVGWCRLVTAAYNYFHVTNFFWMFGEG
  CYLHTAIVLTYSTDRLRKWMFICIGWGVPFPIIVAWAIGKLYYDNEKCWFGKRPGVYTDYIYQGPMILVL
  LINFIFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSF
  LESFQGFFVSVFYCFLNSEVRSAIRKRWHRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004382
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004382.2, NM_004382.3, NM_004382.4, NP_004373.2
• RefSeq Size: 2594 bp
• RefSeq ORF: 1248 bp
• Locus ID: 1394
• Cytogenetics: 17q21.31
• Domains: 7tm_2, HormR
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Long-term depression, Neuroactive ligand-receptor interaction
• MW: 47.7 kDa
• Gene Summary: 'This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Aug 2016]'