CYP3A7 (NM_000765) Human Untagged Clone

CAT#: SC110865

CYP3A7 (untagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 7 (CYP3A7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP3A7
• Synonyms: CP37; CYPIIIA7; P-450(HFL33); P-450111A7; P450-HFLA; P450HLp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000765, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCTCATCCCAAACTTGGCCGTGGAAACCTGGCTTCTCCTGGCTGTCAGCCTGATACTCCTCTATC
  TATATGGAACCCGTACACATGGACTTTTTAAGAAGCTTGGAATTCCAGGGCCCACACCTCTGCCTTTTTT
  GGGAAATGCTTTGTCCTTCCGTAAGGGCTATTGGACGTTTGACATGGAATGTTATAAAAAGTATAGAAAA
  GTCTGGGGTATTTATGACTGTCAACAGCCTATGCTGGCTATCACAGATCCCGACATGATCAAAACAGTGC
  TAGTGAAAGAATGTTATTCTGTCTTCACAAACCGGAGGCCTTTCGGGCCAGTGGGATTTATGAAAAATGC
  CATCTCTATAGCTGAGGATGAAGAATGGAAGAGAATACGATCATTGCTGTCTCCAACATTCACCAGCGGA
  AAACTCAAGGAGATGGTCCCTATCATTGCCCAGTATGGAGATGTGTTGGTGAGAAATCTGAGGCGGGAAG
  CAGAGACAGGCAAGCCTGTCACCTTGAAACACGTCTTTGGGGCCTACAGCATGGATGTGATCACTAGCAC
  ATCATTTGGAGTGAGCATCGACTCTCTCAACAATCCACAAGACCCCTTTGTGGAAAACACCAAGAAGCTT
  TTAAGATTTAATCCATTAGATCCATTCGTTCTCTCAATAAAAGTCTTTCCATTCCTTACCCCAATTCTTG
  AAGCATTAAATATCACTGTGTTTCCAAGAAAAGTTATAAGTTTTCTAACAAAATCTGTAAAACAGATAAA
  AGAAGGTCGCCTCAAAGAGACACAAAAGCACCGAGTGGATTTCCTTCAGCTGATGATTGACTCTCAGAAT
  TCAAAAGACTCTGAGACCCACAAAGCTCTGTCTGATCTGGAGCTCATGGCCCAATCAATTATCTTTATTT
  TTGCTGGCTATGAAACCACGAGCAGTGTTCTCTCCTTCATTATATATGAACTGGCCACTCACCCTGATGT
  CCAGCAGAAAGTGCAGAAGGAAATTGATACAGTTTTACCCAATAAGGCACCACCCACCTATGATACTGTG
  CTACAGTTGGAGTATCTTGACATGGTGGTGAATGAAACACTCAGATTATTCCCAGTTGCTATGAGACTTG
  AGAGGGTCTGCAAAAAAGATGTTGAAATCAATGGGATGTTTATTCCCAAAGGGGTGGTGGTGATGATTCC
  AAGCTATGTTCTTCATCATGACCCAAAGTACTGGAGAGAGCCTGAGAAGTTCCTCCCTGAAAGGTTCAGT
  AAAAAGAACAAGGACAACATAGATCCTTACATATACACACCCTTTGGAAGTGGACCCAGAAACTGCATTG
  GCATGAGGTTTGCTCTCGTGAACATGAAACTTGCTCTAGTCAGAGTCCTTCAGAACTTCTCCTTCAAACC
  TTGTAAAGAAACACAGATCCCCCTGAAATTACGCTTTGGAGGACTTCTTCTAACAGAAAAACCCATTGTT
  CTAAAGGCTGAGTCAAGGGATGAGACCGTAAGTGGAGCCTGA
  
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000765
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000765.2, NP_000756.1
• RefSeq Size: 2080 bp
• RefSeq ORF: 1512 bp
• Locus ID: 1551
• Cytogenetics: 7q22.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3A51P pseudogene and is represented by GeneID:100861540. [provided by RefSeq, Jan 2015]'