DCTN1 (NM_004082) Human Untagged Clone

CAT#: SC110869

DCTN1 (untagged)-Human dynactin 1 (DCTN1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCTN1
• Synonyms: DAP-150; DP-150; P135
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110869 sequence for NM_004082 edited (data generated by NextGen Sequencing)
  ATGGCACAGAGCAAGAGGCACGTGTACAGCCGGACGCCCAGCGGCAGCAGGATGAGTGCG
  GAGGCAAGCGCCCGGCCTCTGCGGGTGGGCTCCCGTGTAGAGGTGATTGGAAAAGGCCAC
  CGAGGCACTGTGGCCTATGTTGGAGCCACACTGTTTGCCACTGGCAAATGGGTAGGCGTG
  ATTCTGGATGAAGCAAAGGGCAAAAATGATGGAACTGTTCAAGGCAGGAAGTACTTCACT
  TGTGATGAAGGGCATGGCATCTTTGTGCGCCAGTCCCAGATCCAGGTATTTGAAGATGGA
  GCAGATACTACTTCCCCAGAGACACCTGATTCTTCTGCTTCAAAAGTCCTCAAAAGAGAG
  GGAACTGATACAACTGCAAAGACTAGCAAACTGCGGGGACTGAAGCCTAAGAAGGCACCG
  ACAGCCCGAAAGACCACAACTCGGCGACCCAAGCCCACGCGCCCAGCCAGTACTGGGGTG
  GCTGGGGCCAGTAGCTCCCTGGGCCCCTCTGGCTCAGCGTCAGCAGGTGAGCTGAGCAGC
  AGTGAGCCCAGCACCCCGGCTCAGACTCCGCTGGCAGCACCCATCATCCCCACGCCGGTC
  CTCACCTCTCCTGGAGCAGTCCCCCCGCTTCCTTCCCCATCCAAGGAGGAGGAGGGACTA
  AGGGCTCAGGTGCGGGACCTGGAGGAGAAACTAGAGACCCTGAGACTGAAACGGGCAGAA
  GACAAAGCAAAGCTAAAAGAGCTGGAGAAACACAAAATCCAGCTGGAGCAGGTGCAGGAA
  TGGAAGAGCAAAATGCAGGAGCAGCAGGCCGACCTGCAGCGGCGCCTCAAGGAGGCGAGA
  AAGGAAGCCAAGGAGGCGCTGGAGGCAAAGGAACGCTATATGGAGGAGATGGCTGATACT
  GCTGATGCCATTGAGATGGCCACTTTGGACAAGGAGATGGCTGAAGAGCGGGCTGAGTCC
  CTGCAGCAGGAGGTGGAGGCACTGAAGGAGCGGGTGGACGAGCTCACTACTGACTTAGAG
  ATCCTCAAGGCTGAGATTGAAGAGAAGGGCTCAGATGGCGCTGCATCCAGTTATCAGCTC
  AAGCAGCTTGAGGAGCAGAATGCCCGCCTGAAGGATGCCCTGGTGAGGATGCGGGATCTT
  TCTTCCTCAGAGAAGCAGGAGCATGTGAAGCTCCAGAAGCTCATGGAAAAGAAGAACCAA
  GAGCTGGAAGTTGTGAGGCAACAGCGGGAGCGTCTGCAGGAGGAGCTAAGCCAGGCAGAG
  AGCACCATTGATGAGCTCAAGGAGCAGGTGGATGCTGCTCTGGGTGCTGAGGAGATGGTG
  GAGATGCTGACAGATCGGAACCTGAATCTGGAAGAGAAAGTGCGCGAGTTGAGGGAGACT
  GTGGGAGACTTGGAAGCGATGAATGAGATGAACGATGAGCTGCAGGAGAATGCACGTGAG
  ACAGAACTGGAGCTGCGGGAGCAGCTGGACATGGCAGGCGCGCGGGTTCGTGAGGCCCAG
  AAGCGTGTGGAGGCAGCCCAGGAGACGGTTGCAGACTACCAGCAGACCATCAAGAAGTAC
  CGCCAGCTGACCGCCCATCTACAGGATGTGAATCGGGAACTGACAAACCAGCAGGAAGCA
  TCTGTGGAGAGGCAACAGCAGCCACCTCCAGAGACCTTTGACTTCAAAATCAAGTTTGCT
  GAGACTAAGGCCCATGCCAAGGCAATTGAGATGGAATTGAGGCAGATGGAGGTGGCCCAG
  GCCAATCGACACATGTCCCTGCTGACAGCCTTCATGCCTGACAGCTTCCTTCGGCCAGGT
  GGGGACCATGACTGCGTTCTGGTGCTGTTGCTCATGCCTCGTCTCATTTGCAAGGCAGAG
  CTGATCCGGAAGCAGGCCCAGGAGAAGTTTGAACTAAGTGAGAACTGTTCAGAGCGGCCT
  GGGCTGCGAGGAGCTGCTGGGGAGCAACTCAGCTTTGCTGCTGGACTGGTGTACTCGCTG
  AGCCTGCTGCAGGCCACGCTACACCGCTATGAGCATGCCCTCTCTCAGTGCAGTGTGGAT
  GTGTATAAGAAAGTGGGCAGCCTGTACCCTGAGATGAGTGCCCATGAGCGCTCCTTGGAT
  TTCCTCATTGAACTGCTGCACAAGGATCAGCTGGATGAGACTGTCAATGTGGAGCCTCTC
  ACCAAGGCCATCAAGTACTATCAGCATCTGTACAGCATCCACCTTGCCGAACAGCCTGAG
  GACTGTACTATGCAGCTGGCTGACCACATTAAGTTCACGCAGAGTGCTCTGGACTGCATG
  AGTGTGGAGGTAGGACGGCTGCGTGCCTTCTTGCAGGGTGGGCAGGAGGCTACAGATATT
  GCCCTCCTGCTCCGGGATCTGGAAACTTCATGCAGTGACATCCGCCAGTTCTGCAAGAAG
  ATCCGAAGGCGAATGCCAGGGACAGATGCTCCTGGGATCCCAGCTGCACTGGCCTTTGGA
  CCACAGGTATCTGACACGCTCCTAGACTGCAGGAAACACTTGACGTGGGTCGTGGCTGTG
  CTGCAGGAGGTGGCAGCTGCTGCTGCCCAGCTCATTGCCCCACTGGCAGAGAATGAGGGG
  CTACTTGTGGCTGCTCTGGAGGAACTGGCTTTCAAAGCAAGCGAGCAGATCTATGGGACC
  CCCTCCAGCAGCCCCTATGAGTGTCTGCGCCAGTCATGCAACATCCTCATCAGTACCATG
  AACAAGCTGGCCACAGCCATGCAGGAGGGGGAGTATGATGCAGAGCGGCCCCCCAGCAAG
  CCTCCACCGGTTGAACTGCGGGCTGCTGCCCTTCGTGCAGAGATCACAGATGCTGAAGGC
  CTGGGTTTGAAGCTCGAAGATCGAGAGACAGTTATTAAGGAGTTGAAGAAGTCACTCAAG
  ATTAAGGGAGAGGAGCTAAGTGAGGCCAATGTGCGGCTGAGCCTCCTGGAGAAGAAGTTG
  GACAGTGCTGCCAAGGATGCAGATGAGCGCATCGAGAAAGTCCAGACTCGGCTGGAGGAG
  ACCCAGGCACTGCTGCGAAAGAAGGAGAAAGAGTTTGAGGAGACAATGGATGCACTCCAG
  GCTGACATCGACCAGCTGGAGGCAGAGAAGGCAGAACTAAAGCAGCGTCTGAACAGCCAG
  TCCAAACGCACGATTGAGGGACTCCGGGGCCCTCCTCCTTCAGGCATTGCTACTCTGGTC
  TCTGGCATTGCTGGTGAAGAACAGCAGCGAGGAGCCATCCCTGGGCAGGCTCCAGGGTCT
  GTGCCAGGCCCAGGGCTGGTGAAGGACTCACCACTGCTGCTTCAGCAGATCTCTGCCATG
  AGGCTGCACATCTCCCAGCTCCAGCATGAGAACAGCATCCTCAAGGGAGCCCAGATGAAG
  GCATCCTTGGCATCCCTGCCCCCTCTGCATGTTGCAAAGCTATCCCATGAGGGCCCTGGC
  AGTGAGTTACCAGCTGGAGCGCTGTATCGTAAGACCAGCCAGCTGCTGGAGACATTGAAT
  CAATTGAGCACACACACGCACGTAGTAGACATCACTCGCACCAGCCCTGCTGCCAAGAGC
  CCGTCGGCCCAACTTATGGAGCAAGTGGCTCAGCTTAAGTCCCTGAGTGACACCGTCGAG
  AAGCTCAAGGATGAGGTCCTCAAGGAGACAGTATCTCAGCGCCCTGGAGCCACAGTACCC
  ACTGACTTTGCCACCTTCCCTTCATCAGCCTTCCTCAGGGCCAAGGAGGAGCAGCAGGAT
  GACACAGTCTACATGGGCAAAGTGACCTTCTCATGTGCGGCTGGTTTTGGACAGCGACAC
  CGGCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGTCGCCTCATCTCCTAA
  
  Clone variation with respect to NM_004082.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004082 unedited
  GGTAAAGGTTCAATACTTTGTNAATACGAACTCACTATNAGGGCAGGCCGCGTAATTCGG
  CACGAGGCTGGGTTCAGGAATCCTCAGGCAGCAGAGGGGACTTGCTCCTCAGCCCCCATC
  ACTGCCCCCACCCCTACCCGTGTAGGCCACCGAGTGAGGCCCATGGACTGGGGAGGGCTG
  TGCCTGACGACTCCTGTGTCCCTCTATGAGGGCCCAGCGCCCTGACCCTCCTGCCTAGGT
  TTCTACCTTTTCTCTCTGTCTTTGGCCAGCTGGGGGAGGGGGTAGAGGCCGGGTGAGCAA
  CATGGCACAGAGCAAGAGGCACGTGTACAGCCGGACGCCCAGCGGCAGCAGGATGAGTGC
  GGAGGCAAGCGCCCGGCCTCTGCGGGTGGGCTCCCGTGTAGAGGTGATTGGAAAAGGCCA
  CCGAGGCACTGTGGCCTATGTTGGAGCCACACTGTTTGCCACTGGCAAATGGGTAGGCGT
  GATTCTGGATGAAGCAAAGGGCAAAAATGATGGAACTGTTCAAGGCAGGAAGTACTTCAC
  TTGTGATGAAGGGCATGGCATCTTTGTGCGCCAGTCCCAGATCCAGGTATTTGAAGATGG
  AGCAGATACTACTTCCCCAGAGACACCTGATTCTTCTGCTTCAAAAGTCCTCAAAAGAGA
  GGGAACTGATACAACTGCAAAGACTAGCAAACTGCGGGGACTGAAGCCTAAGAAGGCACC
  GACAGCCCGAAAGACCACAACTCGGCGACCCAAGCCCACGCGCCCAGCCAGTACTGGGGG
  TAGGCTGGGAGCCAGTAGCCTCCCTGGGGCCCCTCTAGGCTCANCGTCAGCAGGTGAGCT
  GAGCAGCAGTGAGCCCAGCACCCCGGCTCATACTNCGCTGGCAGCACCCATCATCCCCAC
  GCCAGTCCTCANCTCTCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004082 unedited
  NCCTTCTCTGGNACCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTGAAG
  CAGAAGTTGCTTTAATCAAGGGGTGAAGTCCTCAATCAAGGGGACCTCACTTAACCTTTG
  GTGGTGGGGTCTCAGCCTACCCCCCACAAGCCCTGAGGCCCCTCAGGAAGGAGGGAGCAG
  TTGAACAACAAATTATGGCAGAGGCCAGGGAATGGGAGTGGGGGAACCCGGGTCAAGGTG
  AAGGGGCAGGACGCTGAAAGGGTGGGAGTGAAGCTGAACGGGGCAGGAAGAGCTTAACCC
  ACGTTTCTACCTGGGGGCTGGCTGAGGTGGCTGTGCATCGGGCAGAGCGGCACCAGAGGG
  CTGAGGGTCGAAGGGGACAGCAGGGGAAAGGAGTGCTTAGGAGATGAGGCGACTGTGAAG
  CTGGTGCAGCTGCTCCTGGGTCAGCACCAGCCGGTGTCGCTGTCCAAAACCAGCCGCACA
  TGAGAAGGTCACTTTGCCCATGTAGACTGTGTCATCCTGCTGCTCCTCCTTGGCCCTGAG
  GAAGGCTGATGAAGGGAAGGTGGCAAAGTCAGTGGGTACTGTGGCTCCAGGGCGCTGAGA
  TACTGTCTCCTTGAGGACCTCATCCTTGAGCTTCTCGACGGTGTCACTCAGGGACTTAAG
  CTGAGCCACTTGCTCCATAAGTTGGGCCGACGGGCTCTTGGCAGCANGGCTGTTGCGAGT
  GATGTCTACTACGTGCGTGTGTGTGCTCAATTGATTCAATGTCTCCAGCAGCTGGCTGGT
  CTTACGATACAGCGCTCCAGCTTGTAACTCACTGGCAGGGCCCTCATGGGATACCTTTGC
  ACATGCAAAGGGGGCAGGNATGCCAAGGATGCCCTTCTTGGGGCTCCTTGAAGATGGCTG
  TTTCAGCTGGACCTGGGAAAGTGCACCCTATGTCAGAAATCGCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004082
• Insert Size: 4280 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004082.2, NP_004073.2
• RefSeq Size: 4992 bp
• RefSeq ORF: 3837 bp
• Locus ID: 1639
• Cytogenetics: 2p13.1
• Domains: CAP_GLY, M
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• Gene Summary: 'This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (1) has multiple differences in the 5' UTR, 5' coding region and 3' coding region, compared to variant 4. These differences cause translation initiation from an upstream AUG and a protein (isoform 1) with a longer N-terminus containing a CAP-Gly domain and a longer C-terminus, compared to isoform 4. Isoform 1 is also called p150.