DHCR7 (NM_001360) Human Untagged Clone

CAT#: SC110871

DHCR7 (untagged)-Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHCR7
• Synonyms: SLOS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110871 sequence for NM_001360 edited (data generated by NextGen Sequencing)
  ATGGCTGCAAAATCGCAACCCAACATTCCCAAAGCCAAGAGTCTAGATGGCGTCACCAAT
  GACAGAACCGCATCTCAAGGGCAGTGGGGCCGTGCCTGGGAGGTGGACTGGTTTTCACTG
  GCGAGCGTCATCTTCCTACTGCTGTTCGCCCCCTTCATCGTCTACTACTTCATCATGGCT
  TGTGACCAGTACAGCTGCGCCCTGACTGGCCCTGTGGTGGACATCGTCACCGGACATGCT
  CGGCTCTCGGACATCTGGGCCAAGACTCCACCTATAACGAGGAAAGCCGCCCAGCTCTAT
  ACCTTGTGGGTCACCTTCCAGGTGCTTCTGTACACGTCTCTCCCTGACTTCTGCCATAAG
  TTTCTACCCGGCTACGTAGGAGGCATCCAGGAGGGGGCCGTGACTCCTGCAGGGGTTGTG
  AACAAGTATCAGATCAATGGCCTGCAAGCCTGGCTCCTCACGCACCTGCTCTGGTTTGCA
  AACGCTCATCTCCTGTCCTGGTTCTCGCCCACCATCATCTTCGACAACTGGATCCCACTG
  CTGTGGTGCGCCAACATCCTTGGCTATGCCGTCTCCACCTTCGCCATGGTCAAGGGCTAC
  TTCTTCCCCACCAGCGCCAGAGACTGCAAATTCACAGGCAATTTCTTTTACAACTACATG
  ATGGGCATCGAGTTTAACCCTCGGATCGGGAAGTGGTTTGACTTCAAGCTGTTCTTCAAT
  GGGCGCCCCGGGATCGTCGCCTGGACCCTCATCAACCTGTCCTTCGCAGCGAAGCAGCGG
  GAGCTCCACAGCCATGTGACCAATGCCATGGTCCTGGTCAACGTCCTGCAGGCCATCTAC
  GTGATTGACTTCTTCTGGAACGAAACCTGGTACCTGAAGACCATTGACATCTGCCATGAC
  CACTTCGGGTGGTACCTGGGCTGGGGCGACTGTGTCTGGCTGCCTTATCTTTACACGCTG
  CAGGGTCTGTACTTGGTGTACCACCCCGTGCAGCTGTCCACCCCGCACGCCGTGGGCGTC
  CTGCTGCTGGGCCTGGTGGGCTACTACATCTTCCGGGTGGCCAACCACCAGAAGGACCTG
  TTCCGCCGCACGGATGGGCGCTGCCTCATCTGGGGCAGGAAGCCCAAGGTCATCGAGTGC
  TCCTACACATCCGCCGATGGGCAGAGGCACCACAGCAAGCTGCTGGTGTCGGGCTTCTGG
  GGCGTGGCCCGCCACTTCAACTACGTCGGCGACCTGATGGGCAGCCTGGCCTACTGCCTG
  GCCTGTGGCGGCGGCCACCTGCTGCCCTACTTCTACATCATCTACATGGCCATCCTGCTG
  ACCCACCGCTGCCTCCGGGACGAGCACCGCTGCGCCAGCAAGTACGGCCGGGACTGGGAG
  CGCTACACCGCCGCAGTGCCTTACCGCCTGCTGCCTGGAATCTTCTAA
  
  Clone variation with respect to NM_001360.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001360 unedited
  NGGGCAAAATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGCCTCGTGCC
  GAATTCGGCACGAGGGTGGAGCAGCGCGCGCAAGCGAGGCCAGGGGAAGGTGGGCGCAGG
  TGAGGGGCCGAGGTGTGCGCAGGACTTTAGCCGGTTGAGAAGGATCAAGCAGGCATTTGG
  AGCACAGGTGTCTAGAAACTTTTAAGGGGCCGGTTCAAGAAGGAAAAGTTCCCTTCTGCT
  GTGAAACTATTTGGCAAGAGGCTGGAGGGCCCAATGGCTGCAAAATCGCAACCCAACATT
  CCCAAAGCCAAGAGTCTAGATGGCGTCACCAATGACAGAACCGCATCTCAAGGGCAGTGG
  GGCCGTGCCTGGGAGGTGGACTGGTTTTCACTGGCGAGCGTCATCTTCCTACTGCTGTTC
  GCCCCCTTCATCGTCTACTACTTCATCATGGCTTGTGACCAGTACAGCTGCGCCCTGACT
  GGCCCTGTGGTGGACATCGTCACCGGACATGCTCGGCTCTCGGACATCTGGGCCAAGACT
  CCACCTATAACGAGGAAAGCCGCCCAGCTCTATACCTTGTGGGTCACCTTCCAGGTGCTT
  CTGTACACGTCTCTCCCTGACTTCTGCCATAAGTTTCTACCCGGCTACGTAGGAGGCATC
  CAGGAGGGGGCCGTGACTCCTGCAGGGGTTGTGAACAAGTATCAGATCAATGGCCTGCAA
  GCCTGGCTCCTCACGCACCTGCTCTGGTTTGCAAACGCTCATCTCCTGTCCTGGTTCTCG
  CCCACCATCATCTTCGACAACTGGATCCCACTGCTGTGTTGCGCCCACATCCTTGGCTAT
  GCCGTCTCCACCTTCGNCATGGTCAAGGGCTACTTTTTTCCCACCAGGGCCAGAGACTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001360 unedited
  GGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTCTTTTTTTCTTTTTTCTT
  TTTTTTCTTTTTTTCTTTTTTTTTCTTGAACAGGGGGAAATTTAATTTAAAAATGAACTC
  TTCTCGGAGCCTAAAGTTTAAAAAAAGTTTTACCCAACCCCCTTGGGGTGAGGAAAAATG
  CCCCAGGGGAAAAAAGCCCAAAAAAGGGGGGGCCCTCCCGAGGGTGGGACTGAAACCTCC
  TCCCTGGAAAAAGGGGGGGAGGCCCCTGAGGGGGAATTTCCCCGGGTTGGTTGAGCCAAA
  TTTTGCCCAGTCTTTGGGCAAGCAAAAAATTTTTTCGAAAGGGGGGGCGCTCCCAGGGAA
  TTGGGAAACAAAACCCCCCTGCCTTCCCCTGAAAGGGCCCAGCCGGGGCTGGGACCTTTT
  TTAGGGCTGCAAATTCCAGGCAAAACACTCCTGGCAGCTGTGCCCCAGGATCCCGAAGGA
  AACCACCTGAAAGCCCCTTTTTTCCCATGGTCCCAGTTTAACACCGGGCCCCCTCCCAAG
  GGAAAAACGGGCACGGGCCCCCCCCACCATTGTATAATTCGCACGCGAGGGGGTTTCTTA
  TCTCGTAGGCCCTTCCTGGATTCCCCCCCGCCCCCCTCTCCCTTTTTTTTACCACTGATT
  AATTGCCTCTATTGAAAGGGCGTGGGAAACCCCTCTGTTCCCCATTGGGCCTAACTGATC
  CTGTCTCCGTCTCCTAAACGGCAATTTTCTTCCCAGGACTTCTTTAGGTCCCTGTGTTAT
  CCTTTGCCCCCAGATTCCGTTTTTAAATTTACTCTTTGTCGGTACTCCCCATTTCCTCCA
  CCTCCGTCTCCTTATTGCATTACTTTCTAAAACTCCTTTTCATCTTCACTCTTCACTATG
  CCCACTTTCTCTCCTCATACCCCATCTTGTCTCCCCGCTGACCTTATTCTCCTTATCTTG
  TTTATCCGTGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001360
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001360.1, NP_001351.1
• RefSeq Size: 2597 bp
• RefSeq ORF: 1428 bp
• Locus ID: 1717
• Cytogenetics: 11q13.4
• Domains: ERG4_ERG24
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: 'This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.