FEN1 (NM_004111) Human Untagged Clone

CAT#: SC110892

FEN1 (untagged)-Human flap structure-specific endonuclease 1 (FEN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FEN1
• Synonyms: FEN-1; MF1; RAD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110892 sequence for NM_004111 edited (data generated by NextGen Sequencing)
  ATGGGAATTCAAGGCCTGGCCAAACTAATTGCTGATGTGGCCCCCAGTGCCATCCGGGAG
  AATGACATCAAGAGCTACTTTGGCCGTAAGGTGGCCATTGATGCCTCTATGAGCATTTAT
  CAGTTCCTGATTGCTGTTCGCCAGGGTGGGGATGTGCTGCAGAATGAGGAGGGTGAGACC
  ACCAGCCACCTGATGGGCATGTTCTACCGCACCATTCGCATGATGGAGAACGGCATCAAG
  CCCGTGTATGTCTTTGATGGCAAGCCGCCACAGCTCAAGTCAGGCGAGCTGGCCAAACGC
  AGTGAGCGGCGGGCTGAGGCAGAGAAGCAGCTGCAGCAGGCTCAGGCTGCTGGGGCCGAG
  CAGGAGGTGGAAAAATTCACTAAGCGGCTGGTGAAGGTCACTAAGCAGCACAATGATGAG
  TGCAAACATCTGCTGAGCCTCATGGGCATCCCTTATCTTGATGCACCCAGTGAGGCAGAG
  GCCAGCTGTGCTGCCCTGGTGAAGGCTGGCAAAGTCTATGCTGCGGCTACCGAGGACATG
  GACTGCCTCACCTTCGGCAGCCCTGTGCTAATGCGACACCTGACTGCCAGTGAAGCCAAA
  AAGCTGCCAATCCAGGAATTCCACCTGAGCCGGATTCTGCAGGAGCTGGGCCTGAACCAG
  GAACAGTTTGTGGATCTGTGCATCCTGCTAGGCAGTGACTACTGTGAGAGTATCCGGGGT
  ATTGGGCCCAAGCGGGCTGTGGACCTCATCCAGAAGCACAAGAGCATCGAGGAGATCGTG
  CGGCGACTTGACCCCAACAAGTACCCTGTGCCAGAAAATTGGCTCCACAAGGAGGCTCAC
  CAGCTCTTCTTGGAACCTGAGGTGCTGGACCCAGAGTCTGTGGAGCTGAAGTGGAGCGAG
  CCAAATGAAGAAGAGCTGATCAAGTTCATGTGTGGTGAAAAGCAGTTCTCTGAGGAGCGA
  ATCCGCAGTGGGGTCAAGAGGCTGAGTAAGAGCCGCCAAGGCAGCACCCAGGGCCGCCTG
  GATGATTTCTTCAAGGTGACCGGCTCACTCTCTTCAGCTAAGCGCAAGGAGCCAGAACCC
  AAGGGATCCACTAAGAAGAAGGCAAAGACTGGGGCAGCAGGGAAGTTTAAAAGGGGAAAA
  TAA
  
  Clone variation with respect to NM_004111.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004111 unedited
  GACTCACTATAGGCGGCCGCGAATTCGGCACGAGGCGCCGCTAAGCTGAGAAGGGAGAGC
  GAGCTTAGGACCGCCTGCCCGGGGCAACCCCGAACCAAGCTTTAGCCGCCGAGGCCGCGT
  GTCCCAAAGGCCAGTCATCCCTCCTCTGTGTTGCCATGGGAATTCAAGGCCTGGCCAAAC
  TAATTGCTGATGTGGCCCCCAGTGCCATCCGGGAGAATGACATCAAGAGCTACTTTGGCC
  GTAAGGTGGCCATTGATGCCTCTATGAGCATTTATCAGTTCCTGATTGCTGTTCGCCAGG
  GTGGGGATGTGCTGCAGAATGAGGAGGGTGAGACCACCAGCCACCTGATGGGCATGTTCT
  ACCGCACCATTCGCATGATGGAGAACGGCATCAAGCCCGTGTATGTCTTTGATGGCAAGC
  CGCCACAGCTCAAGTCAGGCGAGCTGGCCAAACGCAGTGAGCGGCGGGCTGAGGCAGAGA
  AGCAGCTGCAGCAGGCTCAGGCTGCTGGGGCCGAGCAGGAGGTGGAAAAATTCACTAAGC
  GGCTGGTGAAGGTCACTAAGCAGCACAATGATGAGTGCAAACATCTGCTGAGCCTCATGG
  GCATCCCTTATCTTGATGCACCCANTGAGGCAGAGGCCAGCTGTGCTGCCCTGGTGAAGG
  CTGGCANAGTCTATGCTGCGGCTACCGNAGACATGGACTGCCTCACCTTCGGCAGCCCTG
  TGCTAATGCGACACCTGNACTGCAGTGAAGCCAAAAAGCTGCCCATCCAGGAAATTCACC
  TGAGCCGNATTCTGCANGAGCTGGGCCTGACCAGGAACAGTTTGTGGATCTGTGCATCTG
  CTAGCAGTGACACTGTGAGAGTTCCGGGTATTGGCCCAAGCGGCTGTTGACTCATCAAAG
  CCAGAGCTGNGGGAATCTGCGGGACTACCCACAGTACCTGGCAGAAATGCTCACAGAGCT
  C
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004111 unedited
  TTTAGCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTACTTTTAAAATTTTATTGACTTTTTTCTTCATAACTTTAAAACAAAAACAGCGCA
  TGAAAACCAGTGTCTTATTCCAAAGTCTCAACTCAGCTGATTGCCAGGTGAACATCACCA
  TCTTACTCCTCTGAATAACTAGACACAAATTACATAGCAAGTTCGAGTTTCTGCCCACCC
  AAGACACAGCCAGTAATCAGTCACAAACACAGACACAGCCAACTCCAGGGGCTCCAGCTT
  TCTGCCCATCTTCTCTCAGCAGTTCCTCCCATCTGCTAAGATGCGCCTTCCTGGTGGCTC
  TCTCTCAAGGGGGGTCAAGGCTGAACAAGACAGAAAAGCACAGTCTAGGTCCACCATCAC
  CTCCCACTGGCCACCAGTTGGCCAGCCAGGAAATCATTTCTGTACATCTTTTGTCTCCCC
  CTTTTATCTCCCTCTCTCTTCTCCAAAACTTGTTGCTATCTATCACTTTCATGTAACAAT
  GGACTTAGTGTCCATTAAACTGCCTGAGAAGTGGTTTGAGCCTGACATATTTTCCTGAGC
  TAAAAAAGGAAAAGTACCTCTGTGGCCTTCTTGCCATTAAGATCAAGTAAAAAAGGGACT
  AGCACTACTGAAAAGGGTCACGCTAGAAAAGCCTTAGAATCCTCTCTCCACCCCGTGAAG
  GTTTCTCTAGCTGTAGCTCTTAGGGTACAAGACGGCAAATATTCTGGGGTGAAGGAGGGT
  ATATGGGGGAAACACATTTATTTTTCCCCTTTAAAACTTCCTGCTGCCCCAGTCTTTGCC
  TTCTTCTTANGGGACCCNTNGGGTNTGGCCTCCTTGCGCCTANNCTGAGAGAGTGAGCCG
  GNCACCTTTGAGAATCATCCAGNCGGCCCTGGGTGCTGNCTTGGCGGCTCTTACTCAGCT
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004111
• Insert Size: 2090 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004111.4, NP_004102.1
• RefSeq Size: 2265 bp
• RefSeq ORF: 1143 bp
• Locus ID: 2237
• Cytogenetics: 11q12.2
• Domains: HhH2, XPG_N, XPG_I
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, DNA replication, Non-homologous end-joining
• Gene Summary: 'The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]'