FGF10 (NM_004465) Human Untagged Clone

CAT#: SC110895

FGF10 (untagged)-Human fibroblast growth factor 10 (FGF10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGF10
• Synonyms: fibroblast growth factor 10; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004465, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGAAATGGATACTGACACATTGTGCCTCAGCCTTTCCCCACCTGCCCGGCTGCTGCTGCTGCTGCT
  TTTTGTTGCTGTTCTTGGTGTCTTCCGTCCCTGTCACCTGCCAAGCCCTTGGTCAGGACATGGTGTCACC
  AGAGGCCACCAACTCTTCTTCCTCCTCCTTCTCCTCTCCTTCCAGCGCGGGAAGGCATGTGCGGAGCTAC
  AATCACCTTCAAGGAGATGTCCGCTGGAGAAAGCTATTCTCTTTCACCAAGTACTTTCTCAAGATTGAGA
  AGAACGGGAAGGTCAGCGGGACCAAGAAGGAGAACTGCCCGTACAGCATCCTGGAGATAACATCAGTAGA
  AATCGGAGTTGTTGCCGTCAAAGCCATTAACAGCAACTATTACTTAGCCATGAACAAGAAGGGGAAACTC
  TATGGCTCAAAAGAATTTAACAATGACTGTAAGCTGAAGGAGAGGATAGAGGAAAATGGATACAATACCT
  ATGCATCATTTAACTGGCAGCATAATGGGAGGCAAATGTATGTGGCATTGAATGGAAAAGGAGCTCCAAG
  GAGAGGACAGAAAACACGAAGGAAAAACACCTCTGCTCACTTTCTTCCAATGGTGGTACACTCATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004465 unedited
  GTGCCCATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGGCAGCCACCA
  CCTCGAGCTCTCTCCTTGCCTTGCATCGGGTCTTACCCTTCCAGTATGTTCCTTCTGATG
  AGACAATTTCCAGTGCCGAGAGTTTCAGTACAATGTGGAAATGGATACTGACACATTGTG
  CCTCAGCCTTTCCCCACCTGCCCGGCTGCTGCTGCTGCTGCTTTTTGTTGCTGTTCTTGG
  TGTCTTCCGTCCCTGTCACCTGCCAAGCCCTTGGTCAGGACATGGTGTCACCAGAGGCCA
  CCAACTCTTCTTCCTCCTCCTTCTCCTCTCCTTCCAGCGCGGGAAGGCATGTGCGGAGCT
  ACAATCACCTTCAAGGAGATGTCCGCTGGAGAAAGCTATTCTCTTTCACCAAGTACTTTC
  TCAAGATTGAGAAGAACGGGAAGGTCAGCGGGACCAAGAAGGAGAACTGCCCGTACAGCA
  TCCTGGAGATAACATCAGTAGAAATCGGAGTTGTTGCCGTCAAAGCCATTAACAGCAACT
  ATTACTTAGCCATGAACAAGAAGGGGAAACTCTATGGCTCAAAAGAATNTAACAATGACT
  GTAAGCTGAAGGAGAGGATAGAGGAAAATGGATACAATACCTATGCATCATTTAACTGGC
  AGCATAATGGGAGGCAAATGTATGTGGCATTGAATGGAAAAGGAGCCTTCAGGAGAGGGA
  CAGAAACACGAAGGANAAACACCTCTGCTCACTTTCTCCCATGGTGGTACACTCATAGAG
  GAAGCAACGTTTGTTGATGCAGAAAACCAATGGCTCTTTTGCCAAAATAGTGGATATTCT
  CATGAAGACAGTTGATGAAAGGCAAAGACCCTTGCAAATGTCTGCTTGCTTTAAAGAAGC
  CACCTTTGAAGTTTTTGATTTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004465
• Insert Size: 3710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004465.1, NP_004456.1
• RefSeq Size: 627 bp
• RefSeq ORF: 627 bp
• Locus ID: 2255
• Cytogenetics: 5p12
• Domains: FGF
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton
• Gene Summary: 'The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]'