HMGCR (NM_000859) Human Untagged Clone

CAT#: SC110921

HMGCR (untagged)-Human 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HMGCR
• Synonyms: LDLCQ3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000859, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGGGA
  CAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGATCTGTGGTTGGAA
  TTATGAATGTCCAAAGTTTGAAGAGGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGC
  ATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTGGGTA
  TTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGA
  ATTGACAGGCTTGAATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTA
  GCAAAGTTTGCCCTCAGTTCCAACTCACAGGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTT
  TAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCAGGGGT
  ACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTACTTCGTGTTCATGACT
  TTCTTCCCAGCTTGTGTGTCCTTGGTATTAGAGCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGC
  AGCTCAGCCATTTTGCCCGAGTTTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAA
  GATGATTATGTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCAA
  AACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAGAATTGAACCAA
  GTGTTTCCCTCTGGCAGTTTTATCTCTCTAAAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAG
  TTTAGCTCTCCTTCTGGCTGTCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTA
  AAAAACCCTATCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTA
  TGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAGAAAGAAAAGT
  TGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTGGTTGGTAACTCC
  TCCTTACTCGATACTTCATCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGC
  CTAATGAAGAATGTCTACAGATACTTGGGAATGCAGAGAAAGGTGCAAAATTCCTTAGTGATGCTGAGAT
  CATCCAGTTAGTCAATGCTAAGCATATCCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGT
  GGTGTATCTATTCGCCGACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTT
  ACAGGGATTATAATTACTCCTTGGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCC
  TGTTGGAGTGGCAGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGT
  TGTCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGGTCTTGGTGGAGGTGCCAGCAGCCGAGTCC
  TTGCAGATGGGATGACTCGTGGCCCAGTTGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGC
  CTGGCTCGAAACATCTGAAGGGTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCAGATTTGCACGT
  CTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTTTCCAGTCCAGGTCAGGGGATG
  CCATGGGGATGAACATGATTTCAAAGGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGA
  AATGCAGATTCTAGCCGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAG
  GGAAGAGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGAAGTATTAAAGACTA
  CCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGGCTCTGCCATGGCTGGGAGCATAGG
  AGGCTACAACGCCCATGCAGCAAACATTGTCACCGCCATCTACATTGCCTGTGGACAGGATGCAGCACAG
  AATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCCCACAAATGAAGATTTATATATCA
  GCTGCACCATGCCATCTATAGAGATAGGAACGGTGGGTGGTGGGACCAACCTACTACCTCAGCAAGCCTG
  TTTGCAGATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGCCCGA
  ATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGCAGGACATCTTGTCA
  AAAGTCACATGATTCACAACAGGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGAC
  AGCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000859 unedited
  CCCAATTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGGCGCCGG
  GGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGGATTCTGTAGCTACAATGTTGTC
  AAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGG
  GACAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGAT
  CTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGAGGATGTTTTGAGCAGTGACATTAT
  AATTCTGACAATAACACGATGCATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTT
  ACGTCAACTTGGATCAAAATATATTTTGGGTATTGCTGGCCTTTTCACAATTTTCTCAAG
  TTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGAATTGACAGGCTTGAATGA
  AGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAAAGTT
  TGCCCTCAGTTCCAACTCACAGGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAAT
  TTTAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTAC
  CATGTCAGGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGC
  CAACTACTTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGAGCTTCT
  CTCGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTNTGCCCGAGNTTTAGA
  AGAAGAAGAAATAGCCGAATCCTGTACTCAGAGGGTCAAGATGATATGTCCTAAGCTTGG
  NTCTTGNTCATGCTACAGTCGTGGATAGCTGATCCTCTCTTAAACGTACAGAATCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000859 unedited
  ATGGACCGCGGGCCGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGTAATACAAAAT
  AAGGAGTTCTTTATTATTTATACAAAACTTGTACATTAAAAAAATTAAGATTCAATAACT
  CTGCTGACCCCCTGAGAAAGCTAAGAGCGTTCGTGGGTCCATCAAAGAGCCCTGTGTGAA
  TGTTTCAGTCACCAACCTCCTGGCCACAGGAACTAGGCACAGTTCTAGGGCCATTCACGT
  GGCTCCTTCACTGGCTCTGAAAGCTGACTTTCCCTTTCATTAAGCTCGGCAAGCAAGCCA
  GGGTTTCCTGGACAACATTAAAGAACATTATCTTCTCTGGCCCAGGCTAGAGTATTTTAT
  CAGTACACAAGTTGATTTTTATTTCTTGAACACTTCCAATAAACTAGCATAAGTTTTATT
  ACAACATATACAGATTTGATACAGTTTACAAAAAAAACTAGATTTTTCAACTAAATAAAA
  ATGTCTTTTAAGCAATTAAAGTTGGCTTAGAGACATGGTATTTTTCTTTCAAAACTGTGT
  TTCTACAATGATTTCTAAGGTCCCAGTCTTGCTTGTACTTGACAGTCACCCTCATCTAAG
  CAACATTAAGAGCTCTGATATCTTTAGTAAAGAATACAAAACCCTGTGTTTCTTAAAAGC
  CTAATGCTGAAAGACATGTTATAGCCAATCCCAGACAACATTTATATTTAAACATTTATA
  TTTAAACAAAAGGGCCTCTCTGAACAAATAGCCTGCAGAGATAAATACAGGGATTTGTTT
  TCCTGATAGACTATTTAGCATGTTTACACATTATTCTGAGTTTGGGATAAGAGTGTTTCT
  TCCCTTGAGAAAACAGTCCCCTCTGAGAATATGCTGATACCCCCAAATCAAATAGACAGC
  CCAATGAATTTATTTACAACTGGACTTAACCTACTTTACTCTTGAGTTCCATCCAACTAT
  TATTACTCCAACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000859
• ORF Size: 2667 bp
• Insert Size: 4150
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_000859.1, NP_000850.1
• RefSeq Size: 4471
• RefSeq ORF: 2667
• Locus ID: 3156
• Domains: HMG-CoA_red
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Terpenoid backbone biosynthesis
• Gene Summary: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 3 both encode the same isoform (1).