IDH3B (NM_006899) Human Untagged Clone

CAT#: SC110928

IDH3B (untagged)-Human isocitrate dehydrogenase 3 (NAD+) beta (IDH3B), nuclear gene encoding mitochondrial protein, transcript variant 1


  "NM_006899" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol IDH3B
Synonyms RP46
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_006899, the custom clone sequence may differ by one or more nucleotides


ATGGCGGCATTGAGCGGAGTCCGCTGGCTGACCCGAGCGCTGGTCTCCGCCGGGAACCCTGGGGCATGGA
GAGGTCTGAGTACCTCGGCCGCGGCGCACGCTGCATCGCGGAGCCAGGCCGAGGACGTGAGGGTGGAGGG
CTCCTTTCCCGTGACCATGCTTCCGGGAGACGGTGTGGGGCCTGAGCTGATGCACGCCGTCAAGGAGGTG
TTCAAGGCTGCCGCTGTCCCAGTGGAGTTCCAGGAGCACCACCTGAGTGAGGTGCAGAATATGGCATCTG
AGGAGAAGCTGGAGCAGGTGCTGAGTTCCATGAAGGAGAACAAAGTGGCCATCATTGGAAAGATTCATAC
CCCGATGGAGTATAAGGGGGAGCTAGCCTCCTATGATATGCGGCTGAGGCGTAAGTTGGACTTATTTGCC
AACGTAGTCCATGTGAAGTCACTTCCTGGGTATATGACTCGGCACAACAATCTAGACCTGGTGATCATTC
GAGAGCAGACAGAAGGGGAGTACAGCTCTCTGGAACATGAGAGTGCAAGGGGTGTGATTGAGTGTTTGAA
GATTGTCACACGAGCCAAGTCTCAGCGGATTGCAAAGTTCGCCTTTGACTATGCCACCAAGAAGGGGCGG
GGCAAGGTCACTGCTGTCCACAAGGCCAACATCATGAAACTTGGGGATGGGTTGTTCCTGCAGTGCTGTG
AGGAAGTTGCTGAACTGTACCCCAAAATCAAATTTGAGACAATGATCATAGACAACTGCTGCATGCAGCT
GGTGCAGAATCCTTACCAGTTTGATGTGCTTGTGATGCCCAATCTCTATGGGAACATTATTGACAATCTG
GCTGCTGGCCTGGTTGGGGGAGCTGGTGTGGTCCCTGGTGAGAGCTATAGTGCAGAATACGCAGTCTTTG
AGACGGGTGCCCGGCACCCATTTGCCCAGGCAGTGGGCAGGAATATAGCCAATCCCACGGCCATGCTGCT
GTCGGCTTCCAACATGCTGCGGCATCTTAATCTTGAGTATCACTCCAGCATGATCGCAGATGCGGTGAAG
AAGGTGATCAAAGTTGGCAAGGTGCGGACTCGAGACATGGGCGGCTACAGCACCACAACCGACTTCATCA
AGTCTGTCATCGGTCACCTGCAGACTAAAGGGAGCTAG


>OriGene 5' read for NM_006899 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCCTGCGGGAAACATG
GCGGCATTGAGCGGAGTCCGCTGGCTGACCCGAGCGCTGGTCTCCGCCGGGAACCCTGGG
GCATGGAGAGGTCTGAGTACCTCGGCCGCGGCGCACGCTGCATCGCGGAGCCAGGCCGAG
GACGTGAGGGTGGAGGGCTCCTTTCCCGTGACCATGCTTCCGGGAGACGGTGTGGGGCCT
GAGCTGATGCACGCCGTCAAGGAGGTGTTCAAGGCTGCCGCTGTCCCAGTGGAGTTCCAG
GAGCACCACCTGAGTGAGGTGCAGAATATGGCATCTGAGGAGAAGCTGGAGCAGGTGCTG
AGTTCCATGAAGGAGAACAAAGTGGCCATCATTGGAAAGATTCATACCCCGATGGAGTAT
AAGGGGGAGCTAGCCTCCTATGATATGCGGCTGAGGCGTAAGTTGGACTTATTTGCCAAC
GTAGTCCATGTGAAGTCACTTCCTGGGTATATGACTCGGCACAACAATCTAGACCTGGTG
ATCATTCGAGAGCAGACAGAAGGGGAGTACAGCTCTCTGGAACATGAGAGTGCAAGGGGT
GTGATTGAGTGTTTGAAGATTGTCACACGAGCCAAGTCTCAGCGGATTGGCAAAGTTCGC
CTTTGACTATGCCACCAAGAAGGGGCGGNNGCAGGTCACTGCTGTCCACAAGGCCAACAT
CATGAAACTGGNGATGGGNNNTGTCCTGCANTGCTGTGAGGAAAGTGCTGAACTGTACCC
CAAATCAAATTTGAGACATGATCATAGACCACTGCTGCATGCAGCTGGTGCAAAATCCTT
ACAGNNNTGATGTGCTTGTGATGCCCCATCTCTATGGGAACATATNGACATCTGGCTGCT
GGNCT
>OriGene 3' read for NM_006899 unedited
TATGTACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTCATGTTC
TTTATTGAACACCTTACATGGGTATGGACAGGGCCTATGGGTGGGGCAAGGCAGCAATGA
CAGCCTCAGTGAAGTCATGGCAAGTAGCATAGCCACCCATGTCAGAGGTTCGAACCCAGC
AAGGTGACCATGGTCCACTGCTTAAAGGCACAAGGTCTCTTCCCTGGTACACTGCACTGA
AGGGTATGGGGAGTGTGGTCCTTGCAAGGTTGGAAGAAATAAAGGGCTCTAGCTCCCTTT
AGTCTGCAGGTGACCGATGACAGACTTGATGAAGTCGGTTGTGGTGCTGTAGCCGCCCAT
GTCTCGAGTCCGCACCTTGCCAACTTTGATCACCTTCTTCACCGCATCTGCGATCATGCT
GGAGTGATACTCAAGATTAAGATGCCGCAGCATGTTGGAAGCCGACAGCAGCATGGCCGT
GGGATTGGCTATATTCCTGCCCACTGCCTGGGCAAATGGGTGCCGGGCACCCGTCTCAAA
GACTGCGTATTCTGCACTATAGCTCTCACCAGGGACCACACCAGCTTCCCCAACCAGGCC
AGCAGCCAGATTGTCAATAATGTTCCCATAAAGATTGGGCATCACAAGCACATTAAACCG
GTAAAGATTCTGCACCAACTGCATGCCACAGTTGGCTATGATCATTGTCTAAAATTTGAT
TTTGGGGTACAGGTCACCATACTTTCTTACCAGACTGCCAGAACAACCCTTTCCCAATTT
CATGATGTTGGCCCTGTGGACAGAAGTGAACTTGCCCCGCCCCTTCTTGGGGCCCAACAA
AAGCGAACTTTGCATCCGCTGAGACTTGCTTCGGGGAAA
Restriction Sites NotI-NotI     
ACCN NM_006899
Insert Size 1430 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006899.2, NP_008830.2
RefSeq Size 1561 bp
RefSeq ORF 1158 bp
Locus ID 3420
Cytogenetics 20p13
Domains isodh
Protein Pathways Citrate cycle (TCA cycle), Metabolic pathways
Gene Summary 'Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Sep 2016]'
Transcript Variant: This variant (1) represents the predominate transcript and encodes isoform (a).

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