Eg5 (KIF11) (NM_004523) Human Untagged Clone

CAT#: SC110939

KIF11 (untagged)-Human kinesin family member 11 (KIF11)


  "NM_004523" in other vectors (6)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol KIF11
Synonyms EG5; HKSP; KNSL1; MCLMR; TRIP5
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC110939 sequence for NM_004523 edited (data generated by NextGen Sequencing)
ATGGCGTCGCAGCCAAATTCGTCTGCGAAGAAGAAAGAGGAGAAGGGGAAGAACATCCAG
GTGGTGGTGAGATGCAGACCATTTAATTTGGCAGAGCGGAAAGCTAGCGCCCATTCAATA
GTAGAATGTGATCCTGTACGAAAAGAAGTTAGTGTACGAACTGGAGGATTGGCTGACAAG
AGCTCAAGGAAAACATACACTTTTGATATGGTGTTTGGAGCATCTACTAAACAGATTGAT
GTTTACCGAAGTGTTGTTTGTCCAATTCTGGATGAAGTTATTATGGGCTATAATTGCACT
ATCTTTGCGTATGGCCAAACTGGCACTGGAAAAACTTTTACAATGGAAGGTGAAAGGTCA
CCTAATGAAGAGTATACCTGGGAAGAGGATCCCTTGGCTGGTATAATTCCACGTACCCTT
CATCAAATTTTTGAGAAACTTACTGATAATGGTACTGAATTTTCAGTCAAAGTGTCTCTG
TTGGAGATCTATAATGAAGAGCTTTTTGATCTTCTTAATCCATCATCTGATGTTTCTGAG
AGACTACAGATGTTTGATGATCCCCGTAACAAGAGAGGAGTGATAATTAAAGGTTTAGAA
GAAATTACAGTACACAACAAGGATGAAGTCTATCAAATTTTAGAAAAGGGGGCAGCAAAA
AGGACAACTGCAGCTACTCTGATGAATGCATACTCTAGTCGTTCCCACTCAGTTTTCTCT
GTTACAATACATATGAAAGAAACTACGATTGATGGAGAAGAGCTTGTTAAAATCGGAAAG
TTGAACTTGGTTGATCTTGCAGGAAGTGAAAACATTGGCCGTTCTGGAGCTGTTGATAAG
AGAGCTCGGGAAGCTGGAAATATAAATCAATCCCTGTTGACTTTGGGAAGGGTCATTACT
GCCCTTGTAGAAAGAACACCTCATGTTCCTTATCGAGAATCTAAACTAACTAGAATCCTC
CAGGATTCTCTTGGAGGGCGTACAAGAACATCTATAATTGCAACAATTTCTCCTGCATCT
CTCAATCTTGAGGAAACTCTGAGTACATTGGAATATGCTCATAGAGCAAAGAACATATTG
AATAAGCCTGAAGTGAATCAGAAACTCACCAAAAAAGCTCTTATTAAGGAGTATACGGAG
GAGATAGAACGTTTAAAACGAGATCTTGCTGCAGCCCGTGAGAAAAATGGAGTGTATATT
TCTGAAGAAAATTTTAGAGTCATGAGTGGAAAATTAACTGTTCAAGAAGAGCAGATTGTA
GAATTGATTGAAAAAATTGGTGCTGTTGAGGAGGAGCTGAATAGGGTTACAGAGTTGTTT
ATGGATAATAAAAATGAACTTGACCAGTGTAAATCTGACCTGCAAAATAAAACACAAGAA
CTTGAAACCACTCAAAAACATTTGCAAGAAACTAAATTACAACTTGTTAAAGAAGAATAT
ATCACATCAGCTTTGGAAAGTACTGAGGAGAAACTTCATGATGCTGCCAGCAAGCTGCTT
AACACAGTTGAAGAAACTACAAAAGATGTATCTGGTCTCCATTCCAAACTGGATCGTAAG
AAGGCAGTTGACCAACACAATGCAGAAGCTCAGGATATTTTTGGCAAAAACCTGAATAGT
CTGTTTAATAATATGGAAGAATTAATTAAGGATGGCAGCTCAAAGCAAAAGGCCATGCTA
GAAGTACATAAGACCTTATTTGGTAATCTGCTGTCTTCCAGTGTCTCTGCATTAGATACC
ATTACTACAGTAGCACTTGGATCTCTCACATCTATTCCAGAAAATGTGTCTACTCATGTT
TCTCAGATTTTTAATATGATACTAAAAGAACAATCATTAGCAGCAGAAAGTAAAACTGTA
CTACAGGAATTGATTAATGTACTCAAGACTGATCTTCTAAGTTCACTGGAAATGATTTTA
TCCCCAACTGTGGTGTCTATACTGAAAATCAATAGTCAACTAAAGCATATTTTCAAGACT
TCATTGACAGTGGCCGATAAGATAGAAGATCAAAAAAAGGAACTAGATGGCTTTCTCAGT
ATACTGTGTAACAATCTACATGAACTACAAGAAAATACCATTTGTTCCTTGGTTGAGTCA
CAAAAGCAATGTGGAAACCTAACTGAAGACCTGAAGACAATAAAGCAGACCCATTCCCAG
GAACTTTGCAAGTTAATGAATCTTTGGACAGAGAGATTCTGTGCTTTGGAGGAAAAGTGT
GAAAATATACAGAAACCACTTAGTAGTGTCCAGGAAAATATACAGCAGAAATCTAAGGAT
ATAGTCAACAAAATGACTTTTCACAGTCAAAAATTTTGTGCTGATTCTGATGGCTTCTCA
CAGGAACTCAGAAATTTTAACCAAGAAGGTACAAAATTGGTTGAAGAATCTGTGAAACAC
TCTGATAAACTCAATGGCAACCTGGAAAAAATATCTCAAGAGACTGAACAGAGATGTGAA
TCTCTGAACACAAGAACAGTTTATTTTTCTGAACAGTGGGTATCTTCCTTAAATGAAAGG
GAACAGGAACTTCACAACTTATTGGAGGTTGTAAGCCAATGTTGTGAGGCTTCAAGTTCA
GACATCACTGAGAAATCAGATGGACGTAAGGCAGCTCATGAGAAACAGCATAACATTTTT
CTTGATCAGATGACTATTGATGAAGATAAATTGATAGCACAAAATCTAGAACTTAATGAA
ACCATAAAAATTGGTTTGACTAAGCTTAATTGCTTTCTGGAACAGGATCTGAAACTGGAT
ATCCCAACAGGTACGACACCACAGAGGAAAAGTTATTTATACCCATCAACACTGGTAAGA
ACTGAACCACGTGAACATCTCCTTGATCAGCTGAAAAGGAAACAGCCTGAGCTGTTAATG
ATGCTAAACTGTTCAGAAAACAACAAAGAAGAGACAATTCCGGATGTGGATGTAGAAGAG
GCAGTTCTGGGGCAGTATACTGAAGAACCTCTAAGTCAAGAGCCATCTGTAGATGCTGGT
GTGGATTGTTCATCAATTGGCGGGGTTCCATTTTTCCAGCATAAAAAATCACATGGAAAA
GACAAAGAAAACAGAGGCATTAACACACTGGAGAGGTCTAAAGTGGAAGAAACTACAGAG
CACTTGGTTACAAAGAGCAGATTACCTCTGCGAGCCCAGATCAACCTTTAA

Clone variation with respect to NM_004523.3
>OriGene 5' read for NM_004523 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGATTCGGGCG
GAGACGAGATTAGTGATTTGGCGGCTCCGACTGGCGCGGGACAAACGCCACGGCCAGAGT
ACCGGGTAGAGAGCGGGGACGCCGACCTGCGTGCGTCGGTCCTCCAGGCCACGCCAGCGC
CCGAGAGGGACCAGGGAGACTCCGGCCCCTGTCGGCCGCCAAGCCCCTCCGCCCCTCACA
GCGCCCAGGTCCGCGGCCGGGCCTTGATTTTTTGGCGGGGACCGTCATGGCGTCGCAGCC
AAATTCGTCTGCGAAGAAGAAAGAGGAGAAGGGGAAGAACATCCAGGTGGTGGTGAGATG
CAGACCATTTAATTTGGCAGAGCGGAAAGCTAGCGCCCATTCAATAGTAGAATGTGATCC
TGTACGAAAAGAAGTTAGTGTACGAACTGGAGGATTGGCTGACAAGAGCTCAAGGAAAAC
ATACACTTTTGATATGGTGTTTGGAGCATCTACTAAACAGATTGATGTTTACCGAAGTGT
TGTTTGTCCAATTCTGGATGAAGTTATTATGGGCTATAATTGCACTATCTTTGCGTATGG
CCAAACTGGCACTGGAAAAACTTTTACAATGGAAGGTGAAAGGTCACCTAATGAAGAGTA
TACCTGGGAAGAGGATCCCTTGGCTGGTATAATTCCACGTACCCTTCATCAAATTTTTGA
GAAACTTACTGANTATGGTACTGGATTTTCAGTCAAAGTGTCTCTGTGGGAGATCTANTA
TGAAGAGCTTTTTGATCTTTCTAATCCATCATCTGATGGTTCTGAGAGACTACAGATGTT
TGATGATCCCCCGTACAGAGAGAGTGANTATTAAAGGTTAGAAGAATACCG
Restriction Sites NotI-NotI     
ACCN NM_004523
Insert Size 3870 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004523.2, NP_004514.2
RefSeq Size 4908 bp
RefSeq ORF 3171 bp
Locus ID 3832
Cytogenetics 10q23.33
Domains kinesin
Protein Families Druggable Genome
Gene Summary 'This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]'

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