MDFI (NM_005586) Human Untagged Clone

CAT#: SC110947

MDFI (untagged)-Human MyoD family inhibitor (MDFI)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MDFI
• Synonyms: I-MF; I-mfa
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110947 sequence for NM_005586 edited (data generated by NextGen Sequencing)
  ATGTACCAGGTGAGCGGCCAGCGCCCCTCTGGCTGCGACGCGCCCTATGGAGCCCCCAGC
  GCAGCCCCGGGCCCAGCCCAGACCCTATCCCTCCTTCCTGGGCTGGAGGTAGTAACAGGA
  TCCACTCACCCTGCGGAGGCAGCACCAGAGGAGGGCTCCCTGGAGGAGGCGGCAACCCCC
  ATGCCCCAAGGCAATGGCCCTGGCATCCCCCAGGGCCTGGACAGCACTGACCTCGACGTC
  CCCACAGAAGCTGTGACATGCCAGCCTCAGGGGAACCCCTTGGGCTGCACCCCACTTCTG
  CCGAATGACTCTGGCCACCCCTCAGAGCTGGGCGGCACCAGACGGGCGGGGAATGGTGCC
  CTGGGTGGCCCCAAGGCCCACCGGAAGTTGCAGACACACCCATCTCTCGCCAGCCAGGGC
  AGCAAGAAGAGTAAGAGCAGCAGCAAATCCACCACCTCCCAGATCCCCCTCCAGGCACAG
  GAAGACTGCTGTGTCCACTGCATCCTGTCCTGCCTGTTCTGCGAGTTCCTGACGCTGTGC
  AACATCGTCCTGGACTGCGCCACCTGTGGCTCCTGCAGCTCGGAGGACTCGTGCCTCTGC
  TGCTGCTGCTGTGGCTCTGGCGAGTGTGCCGACTGCGACCTGCCCTGCGACCTGGACTGC
  GGCATCCTGGATGCCTGCTGCGAGTCCGCGGACTGCCTGGAGATCTGCATGGAGTGCTGT
  GGGCTCTGCTTCTCCTCCTGA
  
  Clone variation with respect to NM_005586.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005586 unedited
  GGGGCAACACTTGGTATTTGTNAAACGACTTACTANAGGGNNCGGCCGCGNAATTCGGCA
  CGAGGGGAGAAAGCGCGAGTGAGAGGGGAAGGGGCGCCAGGCGAGCACCCGGGAGCCAGC
  GGGACCTGGGCAGGGGCGCCCGGAGCAGGCGCGCATGGCGGGCCCCGCGCGGGGATCCGG
  CTGGAAGAGAGCGTAGCACGGCTCGCACGAGTCCGGGGCCGATGTACCAGGTGAGCGGCC
  AGCGCCCCTCTGGCTGCGACGCGCCCTATGGAGCCCCCAGCGCAGCCCCGGGCCCAGCCC
  AGACCCTATCCCTCCTTCCTGGGCTGGAGGTAGTAACAGGATCCACTCACCCTGCGGAGG
  CAGCACCAGAGGAGGGCTCCCTGGAGGAGGCGGCAACCCCCATGCCCCAAGGCAATGGCC
  CTGGCATCCCCCAGGGCCTGGACAGCACTGACCTCGACGTCCCCACAGAAGCTGTGACAT
  GCCAGCCTCAGGGGAACCCCTTGGGCTGCACCCCACTTCTGCCGAATGACTCTGGCCACC
  CCTCAGAGCTGGGCGGCACCAGACGGGCGGGGAATGGTGCCCTGGGTGGCCCCAAGGCCC
  ACCGGAAGTTGCAGACACACCCATCTCTCGCCAGCCAGGGCAGCAAGAAGAGTAAGAGCA
  GCAGCAAATCCACCACCTCCCAGATCCCCCTCCAGGCACAGGAAGACTGCTGTGTCCACT
  GCATCCTGTCCTGCCTGTTCTGCGAGTTCCTGACGCTGTGCAACATCGTCCTGGACTGCG
  CCACCTGTGGCTCCTGCAGCTCGGAGGACTCGTGCCTCTGCTGCTGCTGCTGTGGCTCTG
  GNCGAGTGTGCCGACTGCGACCTGCCCTGCGACCTGGACTGCGGCATNCTGGATGCCTGC
  TGCGAGTCCGCGGACTGCTGGAGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_005586
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005586.2, NP_005577.1
• RefSeq Size: 1558 bp
• RefSeq ORF: 741 bp
• Locus ID: 4188
• Cytogenetics: 6p21.1
• Protein Families: Transcription Factors
• Gene Summary: 'This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. It encodes the longer isoform (1). Variants 1, 3, and 4 encode the same isoform (1).