Myosin (MYL5) (NM_002477) Human Untagged Clone

CAT#: SC110955

MYL5 (untagged)-Human myosin, light chain 5, regulatory (MYL5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYL5
• Synonyms: MYLC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002477, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAGCAGGAAGACCAAGAAGAAGGAAGGGGGTGCCCTCCGGGCCCAGAGAGCCTCATCCAATGTCT
  TCTCCAACTTTGAGCAGACTCAGATCCAGGAGTTCAAGGAGGCATTCACACTCATGGATCAGAACCGAGA
  TGGCTTCATTGACAAGGAGGACCTGAAGGACACCTATGCCTCCCTGGGCAAGACCAACGTCAAGGACGAC
  GAGCTGGACGCCATGCTCAAAGAGGCCTCGGGGCCCATCAACTTCACCATGTTTCTGAACCTGTTTGGGG
  AGAAGCTGAGCGGTACCGACGCCGAGGAGACCATTCTTAACGCCTTCAAGATGCTGGACCCGGACGGGAA
  AGGGAAAATCAACAAGGAGTACATCAAGCGTCTGCTGATGTCCCAGGCTGACAAGATGACGGCGGAAGAG
  GTGGACCAGATGTTCCAGTTCGCCTCCATCGATGTGGCGGGCAACCTGGACTACAAGGCGCTCAGCTACG
  TGATCACCCACGGGGAGGAGAAGGAGGAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002477 unedited
  TGTACTACGATTCACTATAGGGCGGCCCGCGATTCGGCACGAGGTGAGCGTGTGTATGTG
  CGTGTGTGTGACCTTGCGGGTGTGGGCTGTGCTGTGTTGTGGGAAGTACGTGCCTCACGT
  TGCTCTCCTGGTGAGAGTCCGGAGCAGGATGAGGGGGTTCCTGGCTTTCACAAAATCCCG
  GTACCCAGAACAAGCCCACCCAGAGTCCACTTGCCCCTCAAGCCTTCAGAGGCCAAGCTG
  GCTCCTGCTGGACGGCGATCCCTGACCACTGTGCTCTGTGGGCCTTCTGGAAGCCACTGT
  CCCTCCCCCAGTCCCTGTGCTGAAGCCAGACACCTGCAGCCGTCCTGCCCCCAACCCCAG
  CTACCCAGGCCTGAGGCTGGCATGCTCCTCAGCTGTCTGGCCCCCTCCAGCCCGAAGGGC
  TGAGGTCCACCCTTCATCTGAGTTAAGTCTCTCAAAACTCTGGGTGCCCTGGAGGGTTTC
  GTCAGCCAGCCCAGGACCCTCAGCCATGGTGCTCCCACCGCAGGCCAGCAGGAAGACCAA
  GAAGAAGGAAGGGGGTGCCCTCCGGGCCCAGAGAGCCTCATCCAATGTCTTCTCCAACTT
  TGAGCAGACTCAGATCCAGGAGTTCAAGGAGGCATTCACACTCATGGATCAGAACCGAGA
  TGGCTTCATTGACAAGGAGGACCTGAAGGACACCTATGCCTCCCTGNGCAAGACCAACGT
  CAAGGACGACGAGCTGGACGCCATGCTCNAAGAGCCTCGGGGCCCATCAACTTCACCATG
  TTTCCTGACCTGTTTGGGGAGAAGCTGAGCGTTACCGACGCCCGAGAGACATTCTTNACG
  CCTTCAGATGCTNGACCCGNCAGGGAAAGGAAATCACAAGAGTACTCACGCGCTGCTGAG
  TCCCAGCTGACAGATGACGCGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002477 unedited
  GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAGGG
  TCCAAGCGTCCAGGTTTATTGACCCGGCTGGGTCTCACTCCTCCTTCTCCTCCCCGGGGG
  TGATCACGTAGCTGAGCGCCTTGTAGTCCAGGTTGCCCGCCACATCGATGGAGGCGAACT
  GGAACATTTGGTCCACCTTTTCCGCCGTCATCTTGTCAGCCTGGGACATCAGCAGACGCT
  TGATGTACTCCTTGTTGATTTTCCCTTTCCCGTCCGGGTCCAGCATCTTGAAGGCGTTAA
  AAATGGTCTCCTCGGCGTCGGTACCGCTCAGCTTCTCCCCAAACAGGTTCAAAAACATGG
  TGAAGTTGATGGGCCCCGAGGCCTCTTTGAGCATGGCGTCCAGCTCGTCGTCCTTGACGT
  TGGTCTTGCCCAGGGAGGCATAGGTGTCCTTCAGGTCCTCCTTGTCAATGAAGCCATCTC
  GGTTCTGATCCATGAGTGTGAATGCCTCCTTGAACTCCTGGATCTGAGTCTGCTCAAAGT
  TGGAGAAGACATATGGATGAGGCTCTCTGGGCCCGGAGGGCACCCCCTTCCTTCTTCTTG
  GTCTTCCTGCTGGCCTGCGGTGGGAGCACCATGGCTGAGGGTCCTGNGCTGGCTGACGAA
  ACCCTCCAGGGCACCCAGAGTTTTGAGAGACTTAACTCAGATGAAGGGTGGACCTCAGCC
  CTTTCGGCTGGAGGGGGCCANACAGCTGAGGAGCATGCCAACCTCAGGCCTGGGTTACCT
  GGGGTTGGGGGCAAGACGGCTGCCAGTTTTTGGCTTAACACAGGCACTTGGGGATGCACA
  GTTGGTTCCAAAAGCCCACCAGACCCAGGGGTCAAGTATCGCCCTCCACCAAGAACCACC
  TTGGCTTCTGAAGCTTGAGGGGAAGTGGATCTTGGTCGCGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002477
• Insert Size: 1060 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002477.1, NP_002468.1
• RefSeq Size: 661 bp
• RefSeq ORF: 522 bp
• Locus ID: 4636
• Cytogenetics: 4p16.3
• Domains: EFh
• Protein Pathways: Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction
• Gene Summary: 'This gene encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene product, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).