NEDD8 (NM_006156) Human Untagged Clone

CAT#: SC110960

NEDD8 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEDD8
• Synonyms: NEDD-8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006156 edited
  GAATTCGGCACGAGGGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACCGGA
  GCGGTAGGAGCAGCAATTTATCCGTGTGCAGCCCCAAACTGGAAAGAAGATGCTAATTAA
  AGTGAAGACGCTGACCGGAAAGGAGATTGAGATTGACATTGAACCTACAGACAAGGTGGA
  GCGAATCAAGGAGCGTGTGGAGGAGAAAGAGGGAATCCCCCCACAACAGCAGAGGCTCAT
  CTACAGTGGCAAGCAGATGAATGATGAGAAGACAGCAGCTGATTACAAGATTTTAGGTGG
  TTCAGTCCTTCACCTGGTGTTGGCTCTGAGAGGAGGAGGTGGTCTTAGGCAGTGATGGAC
  CCTCCATTTTACCTCTTTACCCTGTCGCTCATAATGAGGCATCATATATCCTCTCACTCT
  CTGGGACACCATAGCCACTGCCCCCTCCCCTGGATGCCCAGTAATGTATGTCTACTGGTG
  GGAGACTGTGAGGATCCCAGGATTCAGTATTCCTGGCCCAGAGGGCCCTTGCTGGCTACT
  GGGTGTTAGTTTGCAGTCCTGTGTGCTTCCCTCTCTTATGACTGTGTCCCTGGTTGTCAA
  TAAAATATTTCCTGGCCTCCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006156 unedited
  GGTTGTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGCCCTTG
  CAGCAAGATGCTGGAGGGCGGCAGCGGCGACCGGAGCGGTAGGAGCAGCAATTTATCCGT
  GTGCAGCCCCAANACTGGAAAGAAGATGCTAATTAAAGTGAAGACGCTGACCGGAAAGGA
  GATTGAGATTGACATTGAACCTACAGACAAGGTGGAGCGAATCAAGGAGCGTGTGGAGGA
  GAAAGAGGGAATCCCCCCACAACAGCAGAGGCTCATCTACAGTGGCAAGCAGATGAATGA
  TGAGAAGACAGCAGCTGATTACAAGATTTTAGGTGGTTCAGTCCTTCACCTGGTGTTGGC
  TCTGAGAGGAGGAGGTGGTCTTAGGCAGTGATGGACCCTCCATTTTACCTCTTTACCCTG
  TCGCTCATAATGAGGCATCATATATCCTCTCACTCTCTGGGACACCATAGCCACTGCCCC
  CTCCCCTGGATGCCCAGTAATGTATGTCTACTGGTGGGAGACTGTGAGGATCCCAGGATT
  CAGTATTCCTGGCCCAGAGGGCCCTTGCTGGCTACTGGGTGTTAGTTTGCAGTCCTGTGT
  GCTTCCCTCTCTTATGACTGTGTCCCTGGTTGTCAATAAAATATTTCCTGGCCTCCTGGA
  AAAAAAAAAAANNNNNAAAANNNNAAAAAAAAANAAAAAANAAAAAAAANAAAAAAAAAN
  AAAAAAAANNAAAAAAAANNAANAAAAAAAAAAAAANAAAAAAAAANANNANAAAACAAA
  AAAAAAAAANNCCTCTCCTTTTTTTTTGTGGGCGGGGTTAAATTTTGTTTTTAAAAAAAA
  CCCGGGGGGCTTCTTTTGACCCCCCCCCAAGGTTTTTTTTGGGGCGGGAAGGTCCCCTCC
  GGGGCGCCCCATTTTGTTTATAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006156 unedited
  TATCTTATTTTTAACCAGGGNACACAGCTCATAAGAATAGGGGAAGCACACAGGACTGCA
  AACTAACACCCAGTAGCCAGCAAGGGCCCTCTGGGCCAGGAATACTGAATCCTGGGATCC
  TCACAGTCTCCCACCAGTAGACATACATTACTGGGCATCCAGGGGAGGGGGCAGTGGCTA
  TGGTGTCCCAGAGAGTGAGAGGATATATGATGCCTCATTATGAGCGACAGGGTAAAGAGG
  TGAAATGGAGGGTCCATCACTGCCTAAGACCACCTCCTCCTCTCAGAGCCAACACCAGGT
  GAAGGACTGAACCACCTAAAATCTTGTAATCAGCTGCTGTCTTCTCATCATTCATCTGCT
  TGCCACTGTAGATGAGCCTCTGCTGTTGTGGGGGGATTCCCTCTTTCTCCTCCACACGCT
  CCTTGATTCGCTCCACCTTGTCTGTAGGTTCAATGTCCATCTCAATCTCCTTTCCGGTCA
  GCGTCTTCACTTTAATTACCATCCTCTTTCCAGTTTGGGGCTGCACACGGATAAATTGCT
  GCTCCTACCGCTCCGGTCGCCGCTGCCGCCCTCAGCACTCTTGGTGCATAGGCCCCTCAG
  CCGAATTCCCGGCCGCCTATAATGAGCGTATTAAAAATTCTGACGGGTCCCTAACCGAGC
  CTGCTTATATAGACCCTCCACCGTACCACGCTACCGCCCATTTGCGTAACGGGGCGGGGC
  TATACCACCTTTTGGAAATTCCCGTGATTTTGGGCCCAACAAATTTCCTTTGCTCCAGGG
  GGGGGAACTTTGGAATCCCTGGGTACAACCCTCTCCCCCCCACTGGGGTTTCGCATACCC
  TTTCCCCTGGGATTCCGTGATCATCCCTATATTCCCCCTCACCAAATCCTTTATGTATTC
  CGGCTCCCCCCTTGGGTCCTCCCCGCTGGACTAAAGGGGGGCCTCCTTTAACCACCTGTT
  TTCCCGGCGTTTCGTACATCCCCTTGGTATGAAACCTCTGGCCATGGCATAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006156
• Insert Size: 1870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006156.1, NP_006147.1
• RefSeq Size: 605 bp
• RefSeq ORF: 246 bp
• Locus ID: 4738
• Cytogenetics: 14q12
• Protein Families: Druggable Genome
• Gene Summary: ''