PDE2A (NM_002599) Human Untagged Clone

CAT#: SC110970

PDE2A (untagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE2A
• Synonyms: CGS-PDE; cGSPDE; PDE2A1; PED2A4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002599 edited
  ATGGGGCAGGCATGCGGCCACTCCATCCTCTGCAGGAGCCAGCAGTACCCGGCAGCGCGA
  CCGGCTGAGCCGCGGGGCCAGCAGGTCTTCCTCAAGCCGGACGAGCCGCCGCCGCCGCCG
  CAGCCATGCGCCGACAGCCTGCAGGACGCCTTGCTGAGTCTGGGCTCTGTCATCGACATT
  TCAGGCCTGCAACGTGCTGTCAAGGAGGCCCTGTCAGCTGTGCTCCCCCGAGTGGAAACT
  GTCTACACCTACCTACTGGATGGTGAGTCCCAGCTGGTGTGTGAGGACCCCCCACATGAG
  CTGCCCCAGGAGGGGAAAGTCCGGGAGGCTATCATCTCCCAGAAGCGGCTGGGCTGCAAT
  GGGCTGGGCTTCTCAGACCTGCCAGGGAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCT
  CCTGATACCCAAGTGCTGGTCATGCCGCTAGCGGACAAGGAGGCTGGGGCCGTGGCAGCT
  GTCATCTTGGTGCACTGTGGCCAGCTGAGTGATAATGAGGAATGGAGCCTGCAGGCGGTG
  GAGAAGCATACCCTGGTCGCCCTGCGGAGGGTGCAGGTCCTGCAGCAGCGCGGGCCCAGG
  GAGGCTCCCCGAGCCGTCCAGAACCCCCCGGAGGGGACGGCGGAAGACCAGAAGGGCGGG
  GCGGCGTACACCGACCGCGACCGCAAGATCCTCCAACTGTGCGGGGAACTCTACGACCTG
  GATGCCTCTTCCCTGCAGCTCAAAGTGCTCCAATACCTGCAGCAGGAGACCCGGGCATCC
  CGCTGCTGCCTCCTGCTGGTGTCGGAGGACAATCTCCAGCTTTCTTGCAAGGTCATCGGA
  GACAAAGTGCTCGGGGAAGAGGTCAGCTTTCCCTTGACAGGATGCCTGGGCCAGGTGGTG
  GAAGACAAGAAGTCCATCCAGCTGAAGGACCTCACCTCCGAGGATGTACAACAGCTGCAG
  AGCATGTTGGGCTGTGAGCTGCAGGCCATGCTCTGTGTCCCTGTCATCAGCCGGGCCACT
  GACCAGGTGGTGGCCTTGGCCTGCGCCTTCAACAAGCTAGAAGGAGACTTGTTCACCGAC
  GAGGACGAGCATGTGATCCAGCACTGCTTCCACTACACCAGCACCGTGCTCACCAGCACC
  CTGGCCTTCCAGAAGGAACAGAAACTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCA
  AAGAACCTCTTCACCCACCTGGATGACGTCTCTGTCCTGCTCCAGGAGATCATCACGGAG
  GCCAGAAACCTCAGCAACGCAGAGATCTGCTCTGTGTTCCTGCTGGATCAGAATGAGCTG
  GTGGCCAAGGTGTTCGACGGGGGCGTGGTGGATGATGAGAGCTATGAGATCCGCATCCCG
  GCCGATCAGGGCATCGCGGGACACGTGGCGACCACGGGCCAGATCCTGAACATCCCTGAC
  GCATATGCCCATCCGCTTTTCTACCGCGGCGTGGACGACAGCACCGGCTTCCGCACGCGC
  AACATCCTCTGCTTCCCCATCAAGAACGAGAACCAGGAGGTCATCGGTGTGGCCGAGCTG
  GTGAACAAGATCAATGGGCCATGGTTCAGCAAGTTCGACGAGGACCTGGCGACGGCCTTC
  TCCATCTACTGCGGCATCAGCATCGCCCATTCTCTCCTATACAAAAAAGTGAATGAGGCT
  CAGTATCGCAGCCACCTGGCCAATGAGATGATGATGTACCACATGAAGGTCTCCGACGAT
  GAGTATACCAAACTTCTCCATGATGGGATCCAGCCTGTGGCTGCCATTGACTCCAATTTT
  GCAAGTTTCACCTATACCCCTCGTTCCCTGCCCGAGGATGACACGTCCATGGCCATCCTG
  AGCATGCTGCAGGACATGAATTTCATCAACAACTACAAAATTGACTGCCCGACCCTGGCC
  CGGTTCTGTTTGATGGTGAAGAAGGGCTACCGGGATCCCCCCTACCACAACTGGATGCAC
  GCCTTTTCTGTCTCCCACTTCTGCTACCTGCTCTACAAGAACCTGGAGCTCACCAACTAC
  CTCGAGGACATCGAGATCTTTGCCTTGTTTATTTCCTGCATGTGTCATGACCTGGACCAC
  AGAGGCACAAACAACTCTTTCCAGGTGGCCTCGAAATCTGTGCTGGCTGCGCTCTACAGC
  TCTGAGGGCTCCGTCATGGAGAGGCACCACTTTGCTCAGGCCATCGCCATCCTCAACACC
  CACGGCTGCAACATCTTTGATCATTTCTCCCGGAAGGACTATCAGCGCATGCTGGATCTG
  ATGCGGGACATCATCTTGGCCACAGACCTGGCCCACCATCTCCGCATCTTCAAGGACCTC
  CAGAAGATGGCTGAGGTGGGCTACGACCGAAACAACAAGCAGCACCACAGACTTCTCCTC
  TGCCTCCTCATGACCTCCTGTGACCTCTCTGACCAGACCAAGGGCTGGAAGACTACGAGA
  AAGATCGCGGAGCTGATCTACAAAGAATTCTTCTCCCAGGGAGACCTGGAGAAGGCCATG
  GGCAACAGGCCGATGGAGATGATGGACCGGGAGAAGGCCTATATCCCTGAGCTGCAAATC
  AGCTTCATGGAGCACATTGCAATGCCCATCTACAAGCTGTTGCAGGACCTGTTCCCCAAA
  GCGGCAGAGCTGTACGAGCGCGTGGCCTCCAACCGTGAGCACTGGACCAAGGTGTCCCAC
  AAGTTCACCATCCGCGGCCTCCCAAGTAACAACTCGCTGGACTTCCTGGATGAGGAGTAC
  GAGGTGCCTGATCTGGATGGCACTAGGGCCCCCATCAATGGCTGCTGCAGCCTTGATGCT
  GAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002599 unedited
  TCGCTGAGGAGCGCATTTCAGTGGCAGCGGGNAGAGGCGGGAGCAGCGGGGCAGCATAGC
  TGGATTGGGGTGTTGAGTCCAGGCTGAGTAGGGGGCAGCCCACTGCTCTTGGTCCCTGTG
  CCTGCTGGGGGTGCCCTGCCCTGAACTCCAGGCAGCGGGGACAGGGCGATGTGCCACCTT
  AGTCTGGCTGGGGAGGCGGACGATGAGGAGTGATGGGGCAGGCATGCGGCCACTCCATCC
  TCTGCAGGAGCCAGCAGTACCCGGCAGCGCGACCGGCTGAGCCGCGGGGCCAGCAGGTCT
  TCCTCAAGCCGGACGAGCCGCCGCCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGACG
  CCTTGCTGAGTCTGGGCTCTGTCATCGACATTTCAGGCCTGCAACGTGCTGTCAAGGAGG
  CCCTGTCAGCTGTGCTCCCCCGAGTGGAAACTGTCTACACCTACCTACTGGATGGTGAGT
  CCCAGCTGGTGTGTGAGGACCCCCCACATGAGCTGCCCCAGGAGGGGAAAGTCCGGGAGG
  CTATCATCTCCCATAAGCGGCTGGGCTGCAATGGGCTGGGCTTCTCAGACCTGCCAGGGA
  AGCCCTTGGCCAGGCTGGTGGCTCCACTGGCTCCTGATACCCAAGTGCTGGTCATGCCGC
  TAGCGGACAAGGAGGCTGGGGCCGTGGCAGCTGTCATCCTTGTGCACTGTGGCCAGCTGG
  ATGATAATGAGGAATGGAGCCCTGCAGCGGTGGAGAAGCATACCCTGGTCGCCCTTGCGG
  AGGTGCAGGTCCTGCAGCACCGCGGCCCCAGGGAGGTCTCCCGAACCGTCCAGAACCCCC
  GAAAGGGACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002599 unedited
  ATGGCCGCGGCCGCATCTATATCGAGTTTTTTTTTTTTTTTTTTGGTAGAAAGAAAAGAT
  TTATTCTCATGTACAAAGCGGTCAGCCCACGGGACCATATACGACAGTTGCACAGAGTCC
  TATAAAAACGCATCTCTCTAAAGGCAACTCAGAAAGGTAAGGCAGGTGGACCCCCTCCCC
  CACCCCACAACGCACACAGAATGAAACGGAGAAAAAGAGAGAAGCCAGTGGCCGGGCTGA
  CCCAAGAGTCCCGGCCCTATGGGGTCTCCCAAGCCCCAGGGCACAGGTGGATATGGCCTT
  GAAGAGAGAGCCCTGCCAGGGCTGAAGCCAGGTCTCTCACTGGCTGCAGGAATGGGTAAG
  GGGCTCAGGCCAAGGGGAACACTCAGGGGGCCTCTGAGACACTCAAGGTTGGGCTCTCTG
  TGCCTTAAGGTTGCAGAAACATTCCATAGCCTCTGCTGGGACTCCGCAGAGCGATAGAGC
  TATTTTAGACATTGATCTAAAATGGATCTTATAGCATGGATTGTCTTACCCGCTCCCTGT
  TTGGCCCAGACAGGGGAAGAGACTCGCCCAGCGTCACACAGGCAGGAGGAAGCATGGCTG
  GCTCATTCATACCTGNGCCTGTCTCCAGTGCGGNGATCCCTNCTGCCCTCAATCCATCCT
  GCTTCTCCCAGGGCCCCTCTCTGCAGACACTGAGCTGCCCTCTGCAGCTCTTCCCTGGGC
  CCCTGATCCCCCAGTCCCATCCACATATTTAATAAGTCCCTCAAAGGGGCTTCGCCCCGG
  GTGTTTTTAGTCTCCCAGATAAGGAATGTTTCCAGATGCCACTTGTACTCAAATGCTACC
  TCCTCCTAAAGATCCGAACCCCAGGGGGGGAACCCAAAGGATGACAATATTGGGGGCAAA
  TGGGCAGAAACCTCACGCAAAAAAGCTGGNAATGCCAGGGGCCAGAGCCAATAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002599
• Insert Size: 4600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002599.1, NP_002590.1
• RefSeq Size: 4240 bp
• RefSeq ORF: 2826 bp
• Locus ID: 5138
• Cytogenetics: 11q13.4
• Domains: PDEase, GAF, HDc
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: ''
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform, PDE2A3 (see PMID:9210593). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.