PDK3 (NM_005391) Human Untagged Clone

CAT#: SC110971

PDK3 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 3 (PDK3), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDK3
• Synonyms: CMTX6; GS1-358P8.4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005391 edited
  ATGCGGCTGTTCCGGTGGCTGCTGAAGCAGCCGGTGCCCAAGCAGATCGAGCGCTACTCG
  CGCTTTTCGCCGTCGCCGCTCTCCATCAAACAATTCCTGGACTTCGGGAGAGATAATGCA
  TGTGAGAAAACTTCATATATGTTTCTACGAAAGGAACTTCCTGTGCGGCTGGCTAACACA
  ATGAGAGAAGTTAATCTTCTGCCGGATAATTTACTTAACCGCCCTTCAGTGGGATTGGTT
  CAGAGTTGGTATATGCAGAGTTTTCTTGAACTTTTAGAATATGAAAATAAGAGCCCTGAG
  GATCCACAGGTCTTGGATAACTTTCTACAAGTTCTGATTAAAGTCAGAAATAGACACAAT
  GATGTGGTTCCTACAATGGCACAAGGAGTGATTGAATACAAGGAGAAGTTTGGGTTTGAT
  CCTTTCATTAGCACTAACATCCAATATTTTCTGGATCGGTTTTATACCAACCGCATCTCT
  TTCCGCATGCTTATTAATCAGCACAGAAGTATCGATCCCACCTGTAACGTGGCGGATGTG
  GTGAAAGATGCATATGAAACAGCCAAGATGCTGTGTGAACAGTATTACCTGGTAGCTCCA
  GAGCTGGAAGTTGAAGAATTCAATGCCAAAGCGCCAGACAAACCTATTCAGGTGGTTTAT
  GTGCCCTCACATCTGTTTCATATGCTATTTGAGTTGTTCAAGAACTCAATGAGAGCGACA
  GTTGAACTCTATGAAGACAGAAAAGAGGGCTACCCTGCTGTTAAAACCCTCGTTACTTTG
  GGTAAAGAAGACTTATCCATTAAGATCAGTGACCTAGGTGGTGGTGTCCCACTTCGAAAA
  ATAGATCGTCTTTTTAACTACATGTATTCTACTGCTCCTAGACCCAGCCTGGAGCCTACC
  AGAGCTGCCCCTTTGGCTGGATTTGGTTATGGTTTGCCAATTTCCCGTCTGTATGCTAGA
  TATTTTCAAGGAGATCTGAAACTGTATTCCATGGAAGGAGTGGGTACTGATGCTGTCATT
  TATTTGAAGGCTCTTTCAAGTGAGTCATTTGAGAGACTTCCAGTTTTTAATAAGTCCGCA
  TGGCGCCATTACAAGACCACGCCTGAAGCCGATGATTGGAGCAATCCCAGCAGTGAACCC
  AGGGATGCTTCAAAATACAAAGCAAAACAAGTACAAGATCAAGACTAATAGAACTTTCTA
  GAGGACTGAATGCTGTGGTCCTCTCTGTGAAGAAAGATTGCCTTCTGCAAGTCAAACAGA
  GAGAACTGCTTTCTCCCACCTGCTCTGAGCGTGGCATCACAGTTATTCCTAGTGCTTAAA
  TGTGTATTTTCTCTCTACCACCTGGACTTTCCCAAAGAGTTTTTCCTGTAGCTACTCCAG
  ATCTTCCACTAAAGTAGTAACTTTTTAATGACATCTTGCTGTGGTGTGATTGATGCCTGT
  CACCGAAGAGCACAGAGCCTCCTTACAGCTCTTGTGCCCTCGTACTCTCCATCTTGTTAT
  CATAGCATCTGATGTCTTCAGTCCTTCCAGGATTCTGTGCGCCTTTCCCATTCCAGATAC
  AGCAGATCGTACTGTTGCTCCTTGTGACCAAATACCATATTTGTCCCAAACCTCCACCTA
  GGGCCCTGGGACCCTGAAAGTGGAAGACATCAGGTGGCCATGCCCTGATGATTTAGTCCC
  AGAGCCCTCCGTCTCTCCCGTGCCTCCCTCCGCCACTCTATGATTTAGACTTTTGCCTTC
  TTCCATCTGATCGGATTCTCATTGTGAGATGTGGTGACTTTCATGTTTA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005391 unedited
  CCGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
  TCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCG
  GCACGAGTGCGGCGGCTGCACCGGCGGCGCCGAGGCCGAGATCGAGGCCGGGGTGCGCGC
  TTCGCAAACGTGCCCTATCCGTGCGGCTTGGCTGCGCCAGCCCTTGCGGCCACCCGGGCG
  TCTAGGCGGGTCTGTGCGCCGCCCGGGCGAGGATGCGGCTGTTCCGGTGGCTGCTGAAGC
  AGCCGGTGCCCAAGCAGATCGAGCGCTACTCGCGCTTTTCGCCGTCGCCGCTCTCCATCA
  AACAATTCCTGGACTTCGGGAGAGATAATGCATGTGAGAAAACTTCATATATGTTTCTAC
  GAAAGGAACTTCCTGTGCGGCTGGCTAACACAATGAGAGAAGTTAATCTTCTGCCGGATA
  ATTTACTTAACCGCCCTTCAGTGGGATTGGTTCAGAGTTGGTATATGCAGAGTTTTCTTG
  AACTTTTAGAATATGAAAATAAGAGCCCTGAGGATCCACAGGTCTTGGATAAACTTTCTA
  CAGTTCTGATTAAAAGTCAGAATAGACANCATGATGTGGTTCCCTACATGGCACAAGGAG
  TGATTGGATACAANGAGAAGTTTGGGTTTGATCCTTTCATTAGCACTAACATCCCATATT
  TTCTGGATCGGGGTTATACCAACCGCATCTCTTTTCGCATGCTTATTAATCAGCACAGAA
  GTATCGATCCCACCTGTTACGTGGCGGATGTGGTGAAAGATGCTATGAAACAGCCAGATG
  CTGGTGAACAGTATACCTGGAAGCTCCGAACCTGGAGTTGAAGAATCAATGCAAACGGCC
  GAAAAACTTTAAGGTGGGTTATTTGCCCCCCACCCGTGTAAAGGCCATTTGAGTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005391
• Insert Size: 3100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005391.1, NP_005382.1
• RefSeq Size: 1599 bp
• RefSeq ORF: 1221 bp
• Locus ID: 5165
• Cytogenetics: Xp22.11
• Domains: HATPase_c
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (2) differs at the 3' end compared to variant 1, resulting in a shorter isoform (2) lacking the last 9 aa from the C-terminus, compared to isoform 1.