PPP2R1A (NM_014225) Human Untagged Clone

CAT#: SC110980

PPP2R1A (untagged)-Human protein phosphatase 2, regulatory subunit A, alpha (PPP2R1A), transcript variant 1


  "NM_014225" in other vectors (7)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP2R1A
Synonyms MRD36; PP2A-Aalpha; PP2AA; PP2AAALPHA; PR65A
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC110980 sequence for NM_014225 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGACGAACTC
CGCAATGAGGACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATCGCCTTG
GCCCTTGGGGTTGAAAGGACCCGAAGTGAGCTTCTGCCTTTCCTTACAGATACCATCTAT
GATGAAGATGAGGTCCTCCTGGCCCTGGCAGAACAGCTGGGAACCTTCACTACCCTGGTG
GGAGGCCCAGAGTACGTGCACTGCCTGCTGCCACCGCTGGAGTCGCTGGCCACAGTGGAG
GAGACAGTGGTGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACACGAGCACTCG
CCCTCTGACCTGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGCGACTGG
TTCACCTCCCGCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTGTCCAGT
GCTGTGAAGGCGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACCCCCATG
GTGCGGCGGGCCGCAGCCTCCAAGCTGGGGGAGTTTGCCAAGGTGCTGGAGCTGGACAAC
GTCAAGAGTGAGATCATCCCCATGTTCTCCAACCTGGCCTCTGACGAGCAGGACTCGGTG
CGGCTGCTGGCGGTGGAGGCGTGCGTGAACATCGCCCAGCTTCTGCCCCAGGAGGATCTG
GAGGCCCTGGTGATGCCCACTCTGCGCCAGGCCGCTGAAGACAAGTCCTGGCGCGTCCGC
TACATGGTGGCTGACAAGTTCACAGAGCTCCAGAAAGCAGTGGGGCCTGAGATCACCAAG
ACAGACCTGGTCCCTGCCTTCCAGAACCTGATGAAAGACTGTGAGGCCGAGGTGAGGGCC
GCAGCCTCCCACAAGGTCAAAGAGTTCTGTGAAAACCTCTCAGCTGACTGTCGGGAGAAT
GTGATCATGTCCCAGATCTTGCCCTGCATCAAGGAGCTGGTGTCCGATGCCAACCAACAT
GTCAAGTCTGCCCTGGCCTCAGTCATCATGGGTCTCTCTCCCATCTTGGGCAAAGACAAC
ACCATCGAGCACCTCTTGCCCCTCTTCCTGGCTCAGCTGAAGGATGAGTGCCCTGAGGTA
CGGCTGAACATCATCTCTAACCTGGACTGTGTGAACGAGGTGATTGGCATCCGGCAGCTG
TCCCAGTCCCTGCTCCCTGCCATTGTGGAGCTGGCTGAGGACGCCAAGTGGCGGGTGCGG
CTGGCCATCATTGAGTACATGCCCCTCCTGGCTGGACAGCTGGGAGTGGAGTTCTTTGAT
GAGAAACTTAACTCCTTGTGCATGGCCTGGCTTGTGGATCATGTATATGCCATCCGCGAG
GCAGCCACCAGCAACCTGAAGAAGCTAGTGGAAAAGTTTGGGAAGGAGTGGGCCCATGCC
ACAATCATCCCCAAGGTCTTGGCCATGTCCGGAGACCCCAACTACCTGCACCGCATGACT
ACGCTCTTCTGCATCAATGTGCTGTCTGAGGTCTGTGGGCAGGACATCACCACCAAGCAC
ATGCTACCCACGGTTCTGCGCATGGCTGGGGACCCGGTTGCCAATGTCCGCTTCAATGTG
GCCAAGTCTCTGCAGAAGATAGGGCCCATCCTGGACAACAGCACCTTGCAGAGTGAAGTC
AAGCCCATCCTAGAGAAGCTGACCCAGGACCAGGATGTGGACGTCAAATACTTTGCCCAG
GAGGCTCTGACTGTTCTGTCTCTCGCCTGA

Clone variation with respect to NM_014225.5
>OriGene 5' read for NM_014225 unedited
NGGTTCAGGATTTTGTNATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGTGGC
CGCATCTGACAGGAAGGGACGGAGCCAAGATGGCGGCGGCCGACGGCGACGACTCGCTGT
ACCCCATCGCGGTGCTCATAGACGAACTCCGCAATGAGGACGTTCAGCTTCGCCTCAACA
GCATCAAGAAGCTGTCCACCATCGCCTTGGCCCTTGGGGTTGAAAGGACCCGAAGTGAGC
TTCTGCCTTTCCTTACAGATACCATCTATGATGAAGATGAGGTCCTCCTGGCCCTGGCAG
AACAGCTGGGAACCTTCACTACCCTGGTGGGAGGCCCAGAGTACGTGCACTGCCTGCTGC
CACCGCTGGAGTCGCTGGCCACAGTGGAGGAGACAGTGGTGCGGGACAAGGCAGTGGAGT
CCTTACGGGCCATCTCACACGAGCACTCGCCCTCTGACCTGGAGGCGCACTTTGTGCCGC
TAGTGAAGCGGCTGGCGGGCGGCGACTGGTTCACCTCCCGCACCTCGGCCTGCGGCCTCT
TCTCCGTCTGCTACCCCCGAGTGTCCAGTGCTGTGAAGGCGGAACTTCGACAGTACTTCC
GGAACCTGTGCTCAGATGACACCCCCATGGTGCGGCGGGCCGCAGCCTCCAAGCTGGGGG
AGTTTGCCAAGGTGCTGGAGCTGGACAACGTCAAGAGTGAGATCATCCCCATGTTCTCCA
ACCTGGCCTCTGACGAGCAGGACTCGGTGCGGCTGCTGGCGGTGGNAGCGTGCGTGAACA
TCGCCCAGCTTCTGCCCCAGNAGGATCTGGAGGCCCTGGTGATGCCCACTCTGCGCCAGG
CCGCTGAAGACAGTCCTGGCGCGTNCGCTACATGGTGGCCTGACAGNTCACAGAGCTCCA
GAAAGCATGG
>OriGene 3' read for NM_014225 unedited
GGCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACTTCTAAACCTTTATTTCTCT
GTGAAAAGGGGGAAAATAAAAGGAATAAAATAAAAACGGCACAGTTGACACACAAAAAAA
AACCAATGATGGGGAGGACGGGAGGTGGAGAAGTAAATGGGGGAGGGGTTCCCATTACAG
CAGCAGGATCCAGTGACCCGGGATGCTCACATCTCTCCCTGACGTGGGCGGAGTAGCCCC
TTCCTCCCAAGGTCACTGTCCTGTCCAACCCCGTGCTCCCCTAGCCCGTTGGGAGGTGGA
CAGTGAGACATCTTCCCAGGCTGGGGAGAGGAGGAACCGTGCTGGGGGAAGGGGCCTGGG
GTCAGACCATGCACAGGGAGTGACAGCCAAAGGCCCCCCAGTGTCTCCCCAAAGAGGGAC
TTGTGGGGGTTGGAGGGTGGACACCAGAGGCCAGTGTTTGCTCCTCTTCCAGCATCAGGC
GAGAGACAGAACAGTCAGAGCCTCCTGGGCAAAGTATTTGACGTCCACATCCTGGTCCTG
GGTCAGCTTCTCTAGGATGGGCTTGACTTCCCTCTGCAAGGCGCTGTTGTCCACGATGGG
CCCTATCTTCTGCAGAGACTTGGCCCCATTGAATCGGACATTGGCAACCGGGTCCCCAGC
CATGCCCCTAACCGTGGGTACCCCGTGCTCGGTGGTGATGCCCCGCCCCATACACCTTAT
ATAGTCCCTCGCCCCCCAAACACCGTTTCCTGCCCCCCCTGGTTCTCGGGTCTTTCTGAC
ATGGCCCAAACCTTGCGGCTATTTCGTGTCTGGCCCCCCTCTTTCCCACCCCTCCCTGTC
TTCCTAGGCCCCTGCCGCGTGCCTTCCGCCTGACCCACCCGCCCGAATCCCAGCCTTCCC
CCGATCAACCTCTTCATATCCCCTTTCTCCCGTTTCACCCCAATCCTTGCCTCCCCCCCC
CCCATCCAA
Restriction Sites NotI-NotI     
ACCN NM_014225
Insert Size 2240 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014225.3, NP_055040.2
RefSeq Size 2357 bp
RefSeq ORF 1770 bp
Locus ID 5518
Cytogenetics 19q13.41
Domains HEAT, HEAT_PBS
Protein Families Druggable Genome, Phosphatase, Transcription Factors
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
Gene Summary 'This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.

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