PPP2R1A (NM_014225) Human Untagged Clone

CAT#: SC320858

PPP2R1A (untagged)-Human protein phosphatase 2, regulatory subunit A, alpha (PPP2R1A), transcript variant 1


  "NM_014225" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP2R1A
Synonyms MRD36; PP2A-Aalpha; PP2AA; PP2AAALPHA; PR65A
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_014225.3 GGCACGAGGCAGCGCTGGCCGCAGTCTGACAGGAAAGGGACGGAGCCAAGATGGCGGCGG
CCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGACGAACTCCGCAATGAGG
ACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATCGCCTTGGCCCTTGGGG
TTGAAAGGACCCGAAGTGAGCTTCTGCCTTTCCTTACAGATACCATCTATGATGAAGATG
AGGTCCTCCTGGCCCTGGCAGAACAGCTGGGAACCTTCACTACCCTGGTGGGAGGCCCAG
AGTACGTGCACTGCCTGCTGCCACCGCTGGAGTCGCTGGCCACAGTGGAGGAGACAGTGG
TGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACACGAGCACTCGCCCTCTGACC
TGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGCGACTGGTTCACCTCCC
GCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTGTCCAGTGCTGTGAAGG
CGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACCCCCATGGTGCGGCGGG
CCGCAGCCTCCAAGCTGGGGGAGTTTGCCAAGGTGCTGGAGCTGGACAACGTCAAGAGTG
AGATCATCCCCATGTTCTCCAACCTGGCCTCTGACGAGCAGGACTCGGTGCGGCTGCTGG
CGGTGGAGGCGTGCGTGAACATCGCCCAGCTTCTGCCCCAGGAGGATCTGGAGGCCCTGG
TGATGCCCACTCTGCGCCAGGCCGCTGAAGACAAGTCCTGGCGCGTCCGCTACATGGTGG
CTGACAAGTTCACAGAGCTCCAGAAAGCAGTGGGGCCTGAGATCACCAAGACAGACCTGG
TCCCTGCCTTCCAGAACCTGATGAAAGACTGTGAGGCCGAGGTGAGGGCCGCAGCCTCCC
ACAAGGTCAAAGAGTTCTGTGAAAACCTCTCAGCTGACTGTCGGGAGAATGTGATCATGT
CCCAGATCTTGCCCTGCATCAAGGAGCTGGTGTCCGATGCCAACCAACATGTCAAGTCTG
CCCTGGCCTCAGTCATCATGGGTCTCTCTCCCATCTTGGGCAAAGACAACACCATCGAGC
ACCTCTTGCCCCTCTTCCTGGCTCAGCTGAAGGATGAGTGCCCTGAGGTACGGCTGAACA
TCATCTCTAACCTGGACTGTGTGAACGAGGTGATTGGCATCCGGCAGCTGTCCCAGTCCC
TGCTCCCTGCCATTGTGGAGCTGGCTGAGGACGCCAAGTGGCGGGTGCGGCTGGCCATCA
TTGAGTACATGCCCCTCCTGGCTGGACAGCTGGGAGTGGAGTTCTTTGATGAGAAACTTA
ACTCCTTGTGCATGGCCTGGCTTGTGGATCATGTATATGCCATCCGCGAGGCAGCCACCA
GCAACCTGAAGAAGCTAGTGGAAAAGTTTGGGAAGGAGTGGGCCCATGCCACAATCATCC
CCAAGGTCTTGGCCATGTCCGGAGACCCCAACTACCTGCACCGCATGACTACGCTCTTCT
GCATCAATGTGCTGTCTGAGGTCTGTGGGCAGGACATCACCACCAAGCACATGCTACCCA
CGGTTCTGCGCATGGCTGGGGACCCGGTTGCCAATGTCCGCTTCAATGTGGCCAAGTCTC
TGCAGAAGATAGGGCCCATCCTGGACAACAGCACCTTGCAGAGTGAAGTCAAGCCCATCC
TAGAGAAGCTGACCCAGGACCAGGATGTGGACGTCAAATACTTTGCCCAGGAGGCTCTGA
CTGTTCTGTCTCTCGCCTGATGCTGGAAGAGGAGCAAACACTGGCCTCTGGTGTCCACCC
TCCAACCCCCACAAGTCCCTCTTTGGGGAGACACTGGGGGGCCTTTGGCTGTCACTCCCT
GTGCATGGTCTGACCCCAGGCCCCTTCCCCCAGCACGGTTCCTCCTCTCCCCAGCCTGGG
AAGATGTCTCACTGTCCACCTCCCAACGGGCTAGGGGAGCACGGGGTTGGACAGGACAGT
GACCTTGGGAGGAAGGGGCTACTCCGCCCACGTCAGGGAGAGATGTGAGCATCCCGGGTC
ACTGGATCCTGCTGCTGTAATGGGAACCCCTCCCCCATTTACTTCTCCACCTCCCGTCCT
CCCCATCATTGGTTTTTTTTTGTGTGTCAACTGTGCCGTTTTTATTTTATTCCTTTTATT
TTCCCCCTTTTCACAGAGAAATAAAGGTCTAGAAGTAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_014225
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014225.3, NP_055040.2
RefSeq Size 2357 bp
RefSeq ORF 1770 bp
Locus ID 5518
Cytogenetics 19q13.41
Domains HEAT, HEAT_PBS
Protein Families Druggable Genome, Phosphatase, Transcription Factors
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
Gene Summary 'This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.

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