PPP2R5B (NM_006244) Human Untagged Clone

CAT#: SC110982

PPP2R5B (untagged)-Human protein phosphatase 2, regulatory subunit B', beta (PPP2R5B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R5B
• Synonyms: B56B; B56beta; PR61B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006244 edited
  GCGGCCGCGAATTCGGCACGAGGCCAAGTTAGTCTGTCCAGTCTCACCCAGCACCTCCCA
  GGCCCAGAGAGAACCCCCGGGGCTCTGAAAGCTTGCCCTGCCGCCTGACCGCCATGGAGA
  CGAAGCTGCCCCCTGCAAGCACCCCCACTAGCCCCTCCTCCCCCGGGCTGTCGCCTGTGC
  CCCCACCCGACAAGGTGGACGGCTTCTCCCGCCGTTCCCTCCGCAGAGCCCGGCCCCGCC
  GCTCCCACAGCTCCTCTCAGTTCCGCTATCAGAGCAACCAGCAAGAGCTCACACCGCTGC
  CCCTGCTCAAAGATGTGCCGGCTTCCGAGCTGCACGAGCTGCTGAGCCGGAAGCTGGCCC
  AGTGTGGGGTGATGTTTGACTTCTTGGACTGTGTGGCCGACCTCAAGGGGAAGGAGGTGA
  AGCGGGCAGCCCTCAACGAGCTGGTGGAGTGTGTGGGGAGCACCCGGGGTGTCCTCATCG
  AGCCCGTCTACCCAGACATCATCCGCATGATCTCAGTGAATATCTTCCGGACTCTGCCGC
  CCAGTGAGAACCCTGAATTTGACCCTGAAGAGGATGAGCCCAATCTTGAGCCTTCGTGGC
  CACACCTGCAGCTGGTATATGAGTTTTTCCTGCGTTTCTTGGAGAGCCCAGACTTCCAGC
  CCTCCGTGGCCAAGAGATATGTGGATCAAAAGTTTGTCCTGATGCTCCTGGAGCTATTTG
  ATAGTGAGGATCCCCGGGAGCGTGAGTACCTCAAGACCATCCTGCACCGGGTCTATGGCA
  AGTTCCTGGGTCTCCGGGCCTACATCCGCAAACAGTGCAACCACATCTTCCTCCGGTTCA
  TCTATGAATTCGAGCACTTCAATGGTGTGGCTGAGCTGCTGGAGATCCTAGGAAGCATCA
  TCAATGGCTTTGCGCTGCCCCTGAAGACGGAGCACAAGCAGTTCCTGGTTCGCGTCCTGA
  TCCCCCTGCACTCTGTCAAGTCGCTGTCTGTCTTCCATGCCCAGCTGGCATACTGTGTGG
  TGCAGTTCCTGGAGAAGGATGCCACTCTGACAGAGCACGTGATCCGGGGGCTGCTCAAAT
  ACTGGCCAAAAACCTGCACCCAGAAGGAGGTGATGTTTCTGGGGGAGATGGAAGAGATTC
  TTGATGTCATCGAGCCCTCCCAGTTTGTGAAGATCCAGGAGCCCCTTTTTAAGCAGGTGG
  CTCGCTGTGTTTCCAGCCCCCATTTCCAGGTTGCAGAGCGGGCTCTGTATTTCTGGAACA
  ATGAGTATATCCTAAGCCTCATTGAGGACAACTGCCACACTGTGCTGCCTGCTGTGTTTG
  GGACCCTCTACCAAGTCTCCAAGGAGCACTGGAACCAAACCATCGTATCACTGATCTACA
  ATGTGCTCAAGACCTTCATGGAGATGAATGGGAAGCTGTTTGATGAGCTCACAGCCTCCT
  ACAAGCTGGAAAAGCAGCAGGAGCAGCAGAAGGCCCAGGAGCGTCAGGAGTTATGGCAAG
  GTCTGGAGGAGCTGCGGCTACGCCGGCTACAGGGGACCCAGGGGGCCAAGGAGGCCCCCC
  TCCAGCGGCTTACACCCCAGGTGGCCGCCAGTGGGGGTCAGAGCTAGACAGCACCTCAGA
  AGGGGAAAAGCTAAACCCAGAGCTGTCAGTCCCTCTATCCCTTCTCCTGTCCAGGGGCCC
  AGAGAGAAACACACCTACCCCTGGCCTTGCCAGAGTGGCTTCTGAGGACTCCCTGCCCAG
  CCCAGCTTTCACTGGGGGGAGACGAGGAGAGGCAATGGTGGTCTTGGCAACAGAATGCTC
  AGCCCCTCGTGGCAGGACTTGACAAGGGCAAGCTTGACCAGGAAGCTGCCATCAGGGATC
  TTCCCCTGCCCCGCAAAGCTAGGCTCCAGCTGCAGGCGGGCTCCCACCCTCTGCTCCTGG
  CCTTGGGCAAGGGCACTCAGCGCCTCGCCTGCCCCTGCCTTGGCCAATGCGAGGTCCTTC
  CTTATCCCCACCATGGGGTCCATGGTCTATTTATTCTCGCCCAGCTCACCCTCTACACAG
  ACACTGTCCTGGGTGCACACTCCTCCCTTCCCTCGCTGTGTACTTCCTTGTCCCCTTTTT
  ATTTATTGGGCAGGGGGAGGGGGAGGGCACAGGCAAGAAGAGATTCACAGTGTCCTGGGG
  TAAGGGGGGGTTCACAGTAATCATGGTCTACTCCTCTTTCCGTGGCTGGGGGTAGACTTA
  ATAAAGAGAGAAATTCAAGAAAAAAAAAAAAAAAAAAAAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006244 unedited
  GCATTTGTATACGACNACTATAGGGCGGCCGCGATTCGGCACGAGGCCAAGTTAGTCTGT
  CCAGTCTCACCCAGCACCTCCCAGGCCCAGAGAGAACCCCCGGGGCTCTGAAAGCTTGCC
  CTGCCGCCTGACCGCCATGGAGACGAAGCTGCCCCCTGCAAGCACCCCCACTAGCCCCTC
  CTCCCCCGGGCTGTCGCCTGTGCCCCCACCCGACAAGGTGGACGGCTTCTCCCGCCGTTC
  CCTCCGCAGAGCCCGGCCCCGCCGCTCCCACAGCTCCTCTCAGTTCCGCTATCAGAGCAA
  CCAGCAAGAGCTCACACCGCTGCCCCTGCTCAAAGATGTGCCGGCTTCCGAGCTGCACGA
  GCTGCTGAGCCGGAAGCTGGCCCAGTGTGGGGTGATGTTTGACTTCTTGGACTGTGTGGC
  CGACCTCAAGGGGAAGGAGGTGAAGCGGGCAGCCCTCAACGAGCTGGTGGAGTGTGTGGG
  GAGCACCCGGGGTGTCCTCATCGAGCCCGTCTACCCAGACATCATCCGCATGATCTCAGT
  GAATATCTTCCGGACTCTGCCGCCCAGTGAGAACCCTGAATTTGACCCTGAAGAGGATGA
  GCCCAATCTTGAGCCTTCGTGGCCACACCTGCAGCTGGTATATGAGTTTTTCCTGCGTTT
  CTTGGAGAGCCCAGACTTCCAGCCCTCCGTGGCCAAGAGATATGTGGATCAAAAGTTTGT
  CCTGATGCTCCTGGAGCTATTTGATAGTGAGGATCCCCGGGAGCGTGAGTACCTCAAGAC
  CATCCTGCACCGGGTCTATGGCAAAGTCCTGGGTCTNCGGGCCTACATNCGCAAACAGTG
  CACCACATCTNNCTNCCGTTCATCTATGAAATCGAGCCTTCAATGGGTGTGGCTGAGCTG
  CTGGNAGATCCTANGAAGCATCATCAATGGCTNTGCGCTGCCCCCTGAGACGAGCACAGC
  ATTNCTCGGGNTCCGGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006244 unedited
  TTTAGCTTTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTCTT
  GAATTTCTCTCTTTATTAAGTCTACCCCCAGCCACGGNAAAGAGGAGTAGACCATGATTA
  CTGTGAACCCCCCCTTACCCCAGGACACTGTGAATCTCTTCTTGCCTGTGCCCTCCCCCT
  CCCCCTGCCCAATAAATAAAAAGGGGACAAGGAAGTACACAGCGAGGGAAGGGAGGAGTG
  TGCACCCAGGACAGTGTCTGTGTAGAGGGTGAGCTGGGCGAGAATAAATAGACCATGGAC
  CCCATGGTGGGGATAAGGAAGGACCTCGCATTGGCCAAGGCAGGGGCAGGCGAGGCGCTG
  AGTGCCCTTGCCCAAGGCCAGGAGCAGAGGGTGGGAGCCCGCCTGCAGCTGGAGCCTAGC
  TTTGCGGGGCAGGGGAAGATCCCTGATGGCAGCTTCCTGGTCAAGCTTGCCCTTGTCAAG
  TCCTGCCACGAGGGGCTGAGCATTCTGTTGCCAAGACCACCATTGCCTCTCCTCGTCTCC
  CCCCAGTGAAAGCTGGGCTGGGCAGGGAGTCCTCAGAAGCCACTCTGGCAAGGCCAGGGG
  TAGGTGTGTTTCTCTCTGGGCCCCTGGACAGGAGAAGGGATAGAGGGACTGACAGCTCTG
  GGTTTAGCTTTTCCCCTTCTGAGGTGCTGTCTAGCTCTGACCCCCACTGGCGGCCACCTG
  NGGTGAAGCGCTGAAAAGGGGGCCCTCTTTGCCCCCCTTGGTCCCCTGTATCCCGGCGAA
  CCCGAATTCCTCAAACCTTTGCATAAATCCTGACCCTCTGGGCCTTTTGCTGGTCCTGTT
  GGTTTTCCACCTTGCAGGAAGCCTGTGAGCTCATAAAACGTTTCCCATTATATCTCCATA
  AGGCCTCGACCCCATCGTAAATACCCGAACCACGGGTTTGGCTCCCAGGCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006244
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006244.2, NP_006235.1
• RefSeq Size: 2710 bp
• RefSeq ORF: 1494 bp
• Locus ID: 5526
• Cytogenetics: 11q13.1
• Domains: B56
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• Gene Summary: 'The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily. [provided by RefSeq, Jul 2008]'