Prion protein PrP (PRNP) (NM_000311) Human Untagged Clone

CAT#: SC110984

PRNP (untagged)-Human prion protein (PRNP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRNP
• Synonyms: AltPrP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PrP; PrP27-30; PrP33-35C; PrPc
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110984 sequence for NM_000311 edited (data generated by NextGen Sequencing)
  ATGGCGAACCTTGGCTGCTGGATGCTGGTTCTCTTTGTGGCCACATGGAGTGACCTGGGC
  CTCTGCAAGAAGCGCCCGAAGCCTGGAGGATGGAACACTGGGGGCAGCCGATACCCGGGG
  CAGGGCAGCCCTGGAGGCAACCGCTACCCACCTCAGGGCGGTGGTGGCTGGGGGCAGCCT
  CATGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCCCATGGTGGTGGC
  TGGGGACAGCCTCATGGTGGTGGCTGGGGTCAAGGAGGTGGCACCCACAGTCAGTGGAAC
  AAGCCGAGTAAGCCAAAAACCAACATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCA
  GTGGTGGGGGGCCTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACAT
  TTCGGCAGTGACTATGAGGACCGTTACTATCGTGAAAACATGCACCGTTACCCCAACCAA
  GTGTACTACAGGCCCATGGATGAGTACAGCAACCAGAACAACTTTGTGCACGACTGCGTC
  AATATCACAATCAAGCAGCACACGGTCACCACAACCACCAAGGGGGAGAACTTCACCGAG
  ACCGACGTTAAGATGATGGAGCGCGTGGTTGAGCAGATGTGTATCACCCAGTACGAGAGG
  GAATCTCAGGCCTATTACCAGAGAGGATCGAGCATGGTCCTCTTCTCCTCTCCACCTGTG
  ATCCTCCTGATCTCTTTCCTCATCTTCCTGATAGTGGGATGA
  
  Clone variation with respect to NM_000311.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000311 unedited
  TTTGTATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCGACCGAGAGCAGTCA
  TTATGGCGAACCTTGGCTGCTGGATGCTGGTTCTCTTTGTGGCCACATGGAGTGACCTGG
  GCCTCTGCAAGAAGCGCCCGAAGCCTGGAGGATGGAACACTGGGGGCAGCCGATACCCGG
  GGCAGGGCAGCCCTGGAGGCAACCGCTACCCACCTCAGGGCGGTGGTGGCTGGGGGCAGC
  CTCATGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCCCATGGTGGTG
  GCTGGGGACAGCCTCATGGTGGTGGCTGGGGTCAAGGAGGTGGCACCCACAGTCAGTGGA
  ACAAGCCGAGTAAGCCAAAAACCAACATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGG
  CAGTGGTGGGGGGCCTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATAC
  ATTTCGGCAGTGACTATGAGGACCGTTACTATCGTGAAAACATGCACCGTTACCCCAACC
  AAGTGTACTACAGGCCCATGGATGAGTACAGCAACCAGAACAACTTTGTGCACGACTGCG
  TCAATATCACAATCAAGCAGCACACGGTCACCACAACCACCAAGGGGGAGAACTTCACCG
  AGACCGACGTTAAGATGATGGAGCGCGTGGTTGAGCANATGTGTATCACCCAGTACGAGA
  GGGAATCTCAGGCCTATTACCAGAGGAGATCGAGCATGGTCCTCTTCTCCTCTCCACCTG
  GATCCTNCTGATCTCTTTCCTCATCTTCCTGATAGGGGGATGAGGAANGGTCTTNNCTGT
  TTNCACCATCTTNCTAAATCTTNTTCAGCTTGAGGGAGGCGGNATCCACCTGCACCCTTT
  TANGGGNGGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000311 unedited
  NGGCTTCACTCTGAACCCGCGGCCGCATNCTAAGATCGAGTTTTTTTTTTTTTTTTTTGG
  TGCTCATCTTCGCTCGTTTAATTTCAGTCAGATATTAAACATTTACAATTTTTTTATATA
  ACAAAACAAGAACATGCAAAGTTACAAATATAGAAAATATAAAGTACATGCATATTTCAA
  AGACCTGTTAATGGTGTCCACTTTGGATTCTTACATGAAACGATTCAGTGCACATTGTAA
  GCCTAAGGACCACGCAAAAGGGTTTCCCACATATTAAGTATTCAGTACCTTACAAAAGTT
  AATGCATTAGACACTTCAGATGTTAACTGCTCTAAACAAAACTCCTAAGTCTGTCCTATG
  CAATATATATTTTATATACATATATATTTTTACATAGAATACTCACAAAGTGCAAGCCAA
  TAATAACATTGCAGAAAAGTAATACATATCTGCTAGGTGACAATATCAAACAATTCAGGG
  AATAATTTTACTTTAATTAACATTAACAGAATTTCTTTTTCCACTTCAAATCAATCATAT
  TTCTGTCATCTCCAACCTAAGATATTTTTTAGATTGTCTCCCTATTCTTTGATTCAAAAG
  CCAATTACAGAAACTATGAACTTGACCTAATTCTGGTTTTTGACAATTATGAGACAGAAA
  TAAAGAAATGCAAGCAGTTCTTTTCTTTGCACACTGACCATTTTTTAATTACATCATCCT
  CTATGATGATGGTGCTTTCACAACTGCAGCTCTCCTGTATGTCAAAATCATTCTGGTTTC
  CAGGTAAATGGACAAAGGAGATTTGCCTTCAGTGTCTAGAAAGCAATTACTTTTCAGCTG
  CCTTAATTACTATAGTTAAAGAAAGGATGCCCATATAGGGCCTTTAAACANTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000311
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000311.2, NP_000302.1
• RefSeq Size: 2483 bp
• RefSeq ORF: 762 bp
• Locus ID: 5621
• Cytogenetics: 20p13
• Domains: prion
• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Prion diseases
• Gene Summary: 'The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1-5 can all encode the same isoform (Prp).