SEC13L1 (SEC13) (NM_183352) Human Untagged Clone

CAT#: SC111014

SEC13 (untagged)-Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 1


  "NM_183352" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SEC13
Synonyms D3S1231E; npp-20; SEC13L1; SEC13R
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC111014 sequence for NM_183352 edited (data generated by NextGen Sequencing)
ATGGTGTCAGTAATTAACACTGTGGATACCTCCCATGAGGACATGATTCACGACGCCCAG
ATGGACTACTATGGCACCCGCCTGGCAACCTGCTCATCAGACAGGTCCGTCAAAATCTTT
GATGTGCGCAATGGAGGGCAGATCCTTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTG
TGGCAAGTGGCCTGGGCTCACCCCATGTACGGCAACATCCTGGCATCGTGCTCCTATGAC
CGGAAAGTCATTATCTGGAGAGAGGAAAACGGCACCTGGGAGAAGAGCCACGAGCATGCG
GGACACGACTCCTCAGTGAACTCGGTGTGCTGGGCCCCCCATGACTACGGCCTGATCCTG
GCCTGTGGGAGCTCGGATGGGGCCATCTCCCTGCTGACTTACACCGGGGAAGGCCAATGG
GAAGTAAAGAAGATCAACAACGCTCACACCATTGGCTGCAATGCCGTCAGCTGGGCCCCT
GCTGTTGTACCTGGAAGCCTCATAGACCACCCATCGGGGCAGAAACCCAATTACATCAAG
AGGTTTGCATCAGGTGGCTGTGACAACCTCATCAAGCTGTGGAAGGAGGAGGAGGACGGC
CAGTGGAAGGAGGAGCAGAAGCTAGAAGCGCACAGTGACTGGGTTCGAGATGTGGCCTGG
GCCCCCTCCATCGGCCTGCCCACCAGCACCATCGCCAGCTGCTCCCAGGATGGTCGTGTG
TTCATTTGGACCTGTGATGATGCCTCAAGCAATACGTGGTCCCCTAAATTGTTGCACAAG
TTCAACGATGTGGTGTGGCATGTGAGCTGGTCCATCACAGCCAACATCCTGGCTGTCTCT
GGTGGAGACAATAAGGTGACCCTGTGGAAGGAGTCAGTTGATGGGCAGTGGGTGTGCATC
AGTGATGTCAACAAGGGCCAGGGCTCCGTATCAGCATCAGTGACAGAGGGCCAGCAGAAC
GAGCAGTGA

Clone variation with respect to NM_183352.1
>OriGene 5' read for NM_183352 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCGNCCGGGAGCTGC
CACGTCCGAGACCTGGAGCAGCCACCGCCGCAATCATGGTGTCAGTAATTAACACTGTGG
ATACCTCCCATGAGGACATGATTCACGACGCCCAGATGGACTACTATGGCACCCGCCTGG
CAACCTGCTCATCAGACAGGTCCGTCAAAATCTTTGATGTGCGCAATGGAGGGCAGATCC
TTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTGTGGCAAGTGGCCTGGGCTCACCCCA
TGTACGGCAACATCCTGGCATCGTGCTCCTATGACCGGAAAGTCATTATCTGGAGAGAGG
AAAACGGCACCTGGGAGAAGAGCCACGAGCATGCGGGACACGACTCCTCAGTGAACTCGG
TGTGCTGGGCCCCCCATGACTACGGCCTGATCCTGGCCTGTGGGAGCTCGGATGGGGCCA
TCTCCCTGCTGACTTACACCGGGGAAGGCCAATGGGAAGTAAAGAAGATCAACAACGCTC
ACACCATTGGCTGCAATGCCGTCAGCTGGGCCCCTGCTGTTGTACCTGGAAGCCTCATAG
ACCACCCATCGGGGCAGAAACCCAATTACATCAAGAGGTTTGCATCAGGTGGCTGTGACA
ACCTCATCAAGCTGTGGAAGGAGGAGGANGACGGCCAGTGGAAGGAGGAGCAGAAGCTAG
AAGCGCACAGTGACTGGNGTCGAGATGTGGGCTGGNGCCCCTNCATCGGNCTGCCCACCA
GCACCATCGNCAGCTGCTCCAGGATGGTCGTGTTTCATTTTGACCTGTGATGATGCCTAA
GCATACGTGGTCCCTAAANTGTGCACAAGTCAACGATGTGTGTTGCATGTGAGCTC
>OriGene 3' read for NM_183352 unedited
GCCGCATTCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTATAAGCAATATGACTT
TATTTAGTTCCTTTGGAAACAAAACCCCCCAAAAATGCCTGAACCCAAAGGTACATAAAA
ATGACCCAAAATAGATTTGAACATCACTTGTAGTTTCTTCCTCGTAACATGAGTGCTTTC
AGCTGGACAGTAGATTACAAAGCATCTCCGATCACGTTAAGGCAGATGATCAATCTGTGG
CTGCATCTGTAACTCCTCCTGGGAAAATAATCCTGTTGGAGTTGGGGGCTCTTCCCAGTT
GTTTGGTTAGTTGGCCCAGGAAGGGGCAGTCCTGGAGCTGGCGGGTGGGGAGCCAGGCCC
CACCTGTCTTGTCACTGCTCGTTCTGCTGGCCCTCTGTCACTGATGCTGATACGGAGCCC
TGGCCCTTGTTGACATCACTGATGCACACCCACTGCCCATCAACTGACTCCTTCCACAGG
GTCACCTTATTGTCTCCACCAGAGACAGCCAGGATGTTGGCTGTGATGGACCANCTCACA
TGCCACACCACATCGTTGAACTTGTGCAACAATTTAGGGGACCACGTATTGCTTGAGGCA
TCATCACAGGTCCAAATGAACACACGACCATCCTGGGAGCANCTGGCGATGGTGCTGGTG
GGCAGGCCGATGGAGGGGGCCCAGGCCACATCTCGAACCCAGTCACTGTGCGCTTCTAGC
TTCTGCTCCTCCTTACACTGGCCGGGCTCCTCCTTCTTCCACAGTTTGATGAAGGTTGTC
ACAGCCCCTGATGCCAACCTTTGAAGTAATCGGTCTCTGCCCCGATGGCTGGTCTATGAG
CCTTCAAGTACACAGCAAGGGCCCACCTGACGCATTGCACCAATGTGTAGCGTTGTGATC
TTTTTACTTCCATTGGCTTTCCCGGGTAGTCACAAGGAGAGGCCCCATCCACTCCCCAGG
CAGTATAGGCGTATTCTGTGGGCCCCCCAG
Restriction Sites NotI-NotI     
ACCN NM_183352
Insert Size 1410 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_183352.1, NP_899195.1
RefSeq Size 1428 bp
RefSeq ORF 969 bp
Locus ID 6396
Cytogenetics 3p25.3
Gene Summary 'The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2008]'
Transcript Variant: This variant (1) differs in the 5' UTR and lacks an exon in the 5' coding region, compared to variant 3. It encodes isoform 1 which has a shorter and distinct N-terminus, compared to isoform 3.

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