SEC13L1 (SEC13) (NM_183352) Human Untagged Clone

CAT#: SC111014

SEC13 (untagged)-Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEC13
• Synonyms: D3S1231E; npp-20; SEC13L1; SEC13R
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111014 sequence for NM_183352 edited (data generated by NextGen Sequencing)
  ATGGTGTCAGTAATTAACACTGTGGATACCTCCCATGAGGACATGATTCACGACGCCCAG
  ATGGACTACTATGGCACCCGCCTGGCAACCTGCTCATCAGACAGGTCCGTCAAAATCTTT
  GATGTGCGCAATGGAGGGCAGATCCTTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTG
  TGGCAAGTGGCCTGGGCTCACCCCATGTACGGCAACATCCTGGCATCGTGCTCCTATGAC
  CGGAAAGTCATTATCTGGAGAGAGGAAAACGGCACCTGGGAGAAGAGCCACGAGCATGCG
  GGACACGACTCCTCAGTGAACTCGGTGTGCTGGGCCCCCCATGACTACGGCCTGATCCTG
  GCCTGTGGGAGCTCGGATGGGGCCATCTCCCTGCTGACTTACACCGGGGAAGGCCAATGG
  GAAGTAAAGAAGATCAACAACGCTCACACCATTGGCTGCAATGCCGTCAGCTGGGCCCCT
  GCTGTTGTACCTGGAAGCCTCATAGACCACCCATCGGGGCAGAAACCCAATTACATCAAG
  AGGTTTGCATCAGGTGGCTGTGACAACCTCATCAAGCTGTGGAAGGAGGAGGAGGACGGC
  CAGTGGAAGGAGGAGCAGAAGCTAGAAGCGCACAGTGACTGGGTTCGAGATGTGGCCTGG
  GCCCCCTCCATCGGCCTGCCCACCAGCACCATCGCCAGCTGCTCCCAGGATGGTCGTGTG
  TTCATTTGGACCTGTGATGATGCCTCAAGCAATACGTGGTCCCCTAAATTGTTGCACAAG
  TTCAACGATGTGGTGTGGCATGTGAGCTGGTCCATCACAGCCAACATCCTGGCTGTCTCT
  GGTGGAGACAATAAGGTGACCCTGTGGAAGGAGTCAGTTGATGGGCAGTGGGTGTGCATC
  AGTGATGTCAACAAGGGCCAGGGCTCCGTATCAGCATCAGTGACAGAGGGCCAGCAGAAC
  GAGCAGTGA
  
  Clone variation with respect to NM_183352.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_183352 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCGNCCGGGAGCTGC
  CACGTCCGAGACCTGGAGCAGCCACCGCCGCAATCATGGTGTCAGTAATTAACACTGTGG
  ATACCTCCCATGAGGACATGATTCACGACGCCCAGATGGACTACTATGGCACCCGCCTGG
  CAACCTGCTCATCAGACAGGTCCGTCAAAATCTTTGATGTGCGCAATGGAGGGCAGATCC
  TTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTGTGGCAAGTGGCCTGGGCTCACCCCA
  TGTACGGCAACATCCTGGCATCGTGCTCCTATGACCGGAAAGTCATTATCTGGAGAGAGG
  AAAACGGCACCTGGGAGAAGAGCCACGAGCATGCGGGACACGACTCCTCAGTGAACTCGG
  TGTGCTGGGCCCCCCATGACTACGGCCTGATCCTGGCCTGTGGGAGCTCGGATGGGGCCA
  TCTCCCTGCTGACTTACACCGGGGAAGGCCAATGGGAAGTAAAGAAGATCAACAACGCTC
  ACACCATTGGCTGCAATGCCGTCAGCTGGGCCCCTGCTGTTGTACCTGGAAGCCTCATAG
  ACCACCCATCGGGGCAGAAACCCAATTACATCAAGAGGTTTGCATCAGGTGGCTGTGACA
  ACCTCATCAAGCTGTGGAAGGAGGAGGANGACGGCCAGTGGAAGGAGGAGCAGAAGCTAG
  AAGCGCACAGTGACTGGNGTCGAGATGTGGGCTGGNGCCCCTNCATCGGNCTGCCCACCA
  GCACCATCGNCAGCTGCTCCAGGATGGTCGTGTTTCATTTTGACCTGTGATGATGCCTAA
  GCATACGTGGTCCCTAAANTGTGCACAAGTCAACGATGTGTGTTGCATGTGAGCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_183352 unedited
  GCCGCATTCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTATAAGCAATATGACTT
  TATTTAGTTCCTTTGGAAACAAAACCCCCCAAAAATGCCTGAACCCAAAGGTACATAAAA
  ATGACCCAAAATAGATTTGAACATCACTTGTAGTTTCTTCCTCGTAACATGAGTGCTTTC
  AGCTGGACAGTAGATTACAAAGCATCTCCGATCACGTTAAGGCAGATGATCAATCTGTGG
  CTGCATCTGTAACTCCTCCTGGGAAAATAATCCTGTTGGAGTTGGGGGCTCTTCCCAGTT
  GTTTGGTTAGTTGGCCCAGGAAGGGGCAGTCCTGGAGCTGGCGGGTGGGGAGCCAGGCCC
  CACCTGTCTTGTCACTGCTCGTTCTGCTGGCCCTCTGTCACTGATGCTGATACGGAGCCC
  TGGCCCTTGTTGACATCACTGATGCACACCCACTGCCCATCAACTGACTCCTTCCACAGG
  GTCACCTTATTGTCTCCACCAGAGACAGCCAGGATGTTGGCTGTGATGGACCANCTCACA
  TGCCACACCACATCGTTGAACTTGTGCAACAATTTAGGGGACCACGTATTGCTTGAGGCA
  TCATCACAGGTCCAAATGAACACACGACCATCCTGGGAGCANCTGGCGATGGTGCTGGTG
  GGCAGGCCGATGGAGGGGGCCCAGGCCACATCTCGAACCCAGTCACTGTGCGCTTCTAGC
  TTCTGCTCCTCCTTACACTGGCCGGGCTCCTCCTTCTTCCACAGTTTGATGAAGGTTGTC
  ACAGCCCCTGATGCCAACCTTTGAAGTAATCGGTCTCTGCCCCGATGGCTGGTCTATGAG
  CCTTCAAGTACACAGCAAGGGCCCACCTGACGCATTGCACCAATGTGTAGCGTTGTGATC
  TTTTTACTTCCATTGGCTTTCCCGGGTAGTCACAAGGAGAGGCCCCATCCACTCCCCAGG
  CAGTATAGGCGTATTCTGTGGGCCCCCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_183352
• Insert Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183352.1, NP_899195.1
• RefSeq Size: 1428 bp
• RefSeq ORF: 969 bp
• Locus ID: 6396
• Cytogenetics: 3p25.3
• Gene Summary: 'The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (1) differs in the 5' UTR and lacks an exon in the 5' coding region, compared to variant 3. It encodes isoform 1 which has a shorter and distinct N-terminus, compared to isoform 3.