SOX10 (NM_006941) Human Untagged Clone

CAT#: SC111023

SOX10 (untagged)-Human SRY (sex determining region Y)-box 10 (SOX10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SOX10
• Synonyms: DOM; PCWH; WS2E; WS4; WS4C
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111023 sequence for NM_006941 edited (data generated by NextGen Sequencing)
  ATGGCGGAGGAGCAGGACCTATCGGAGGTGGAGCTGAGCCCCGTGGGCTCGGAGGAGCCC
  CGCTGCCTGTCCCCGGGGAGCGCGCCCTCGCTAGGGCCCGACGGCGGCGGCGGCGGATCG
  GGCCTGCGAGCCAGCCCGGGGCCAGGCGAGCTGGGCAAGGTCAAGAAGGAGCAGCAGGAC
  GGCGAGGCGGACGATGACAAGTTCCCCGTGTGCATCCGCGAGGCCGTCAGCCAGGTGCTC
  AGCGGCTACGACTGGACGCTGGTGCCCATGCCCGTGCGCGTCAACGGCGCCAGCAAAAGC
  AAGCCGCACGTCAAGCGGCCCATGAACGCCTTCATGGTGTGGGCTCAGGCAGCGCGCAGG
  AAGCTCGCGGACCAGTACCCGCACCTGCACAACGCTGAGCTCAGCAAGACGCTGGGCAAG
  CTCTGGAGGCTGCTGAACGAAAGTGACAAGCGCCCCTTCATCGAGGAGGCTGAGCGGCTC
  CGTATGCAGCACAAGAAAGACCACCCGGACTACAAGTACCAGCCCAGGCGGCGGAAGAAC
  GGGAAGGCCGCCCAGGGCGAGGCGGAGTGCCCCGGTGGGGAGGCCGAGCAAGGTGGGACC
  GCCGCCATCCAGGCCCACTACAAGAGCGCCCACTTGGACCACCGGCACCCAGGAGAGGGC
  TCCCCCATGTCAGATGGTAACCCCGAGCACCCCTCAGGCCAGAGCCATGGCCCACCCACC
  CCTCCAACCACCCCGAAGACAGAGCTGCAGTCGGGCAAGGCAGACCCGAAGCGGGACGGG
  CGCTCCATGGGGGAGGGCGGGAAGCCTCACATCGACTTCGGCAACGTGGACATTGGTGAG
  ATCAGCCACGAGGTAATGTCCAACATGGAGACCTTTGATGTGGCTGAGTTGGACCAGTAC
  CTGCCGCCCAATGGGCACCCAGGCCATGTGAGCAGCTACTCAGCAGCCGGCTATGGGCTG
  GGCAGTGCCCTGGCCGTGGCCAGTGGACACTCCGCCTGGATCTCCAAGCCACCAGGCGTG
  GCTCTGCCCACGGTCTCACCACCTGGTGTGGATGCCAAAGCCCAGGTGAAGACAGAGACC
  GCGGGGCCCCAGGGGCCCCCACACTACACCGACCAGCCATCCACCTCACAGATCGCCTAC
  ACCTCCCTCAGCCTGCCCCACTATGGCTCAGCCTTCCCCTCCATCTCCCGCCCCCAGTTT
  GACTACTCTGACCATCAGCCCTCAGGACCCTATTATGGCCACTCGGGCCAGGCCTCTGGC
  CTCTACTCGGCCTTCTCCTATATGGGGCCCTCGCAGCGGCCCCTCTACACGGCCATCTCT
  GACCCCAGCCCCTCAGGGCCCCAGTCCCACAGCCCCACACACTGGGAGCAGCCAGTATAT
  ACGACACTGTCCCGGCCCTAA
  
  Clone variation with respect to NM_006941.3
  678 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006941 unedited
  AACTCGAGAATTGTTAAACGACTACTATAGGCGGCCGCGCAATTCGCACGAGGTCCGGCC
  GGGTGGTTGGTGGTAAGGATTCAGGCTCCGTCCTAACGAGGCCGTGGCCTGAGGCTCAGG
  GCCCCCCGCCCCTCCCTCCCAGCCCACCAGCGTCACCTCCCAGCCCCGAGCTGGACCGCA
  CACCTTGGGACACGGTTTTCCACTTCCTAAGGACGAGCCCCAGACTGGAGGAGAGGTCCG
  AGGAGGTGGGCGTTGGACTCTTTGCGAGGACCCCGGCGGCTGGCCCGGGGGAGGCGGCCG
  AGGCGGCGGCGGCGGCGGCCGGGGGCGACATGGCGGAGGAGCAGGACCTATCGGAGGTGG
  AGCTGAGCCCCGTGGGCTCGGAGGAGCCCCGCTGCCTGTCCCCGGGGAGCGCGCCCTCGC
  TAGGGCCCGACGGCGGCGGCGGCGGATCGGGCCTGCGAGCCAGCCCGGGGCCAGGCGAGC
  TGGGCAAGGTCAAGAAGGAGCAGCAGGACGGCGAGGCGGACGATGACAAGTTCCCCGTGT
  GCATCCGCGAGGCCGTCAGCCAGGTGCTCAGCGGCTACGACTGGACGCTGGTGCCCATGC
  CCGTGCGCGTCAACGGCGCCAGCAAAAGCAAGCCGCACGTCAAGCGGCCCATGAACGCCT
  TCATGGTGTGGGCTCAGGCAGCGCGCAGGAAGCTCGCGGACCAGTACCCGCACCTGCACA
  ACGCTGAGCTCAGCAAGACGCTGGGCAAGCTCTGGAGGCTGCTGNACGAAAGTGACAAGC
  GCCCCTTCATCGAGGAGGCTGAGCGGCTCCGTATGCAGCACAAGAAAGACCACCGGACTA
  CCAGTACCAGCCCCCAGCGCGGAAGAACGGGAAGGCCGNCCCCAGGCANNGCGATGCCCC
  CCGTNGGGGAGGCCCGAACAGGGTGGGACCGCCGNCATCCCAGCCCACTACNAGAGCC
  
• Restriction Sites: Please inquire
• ACCN: NM_006941
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006941.3, NP_008872.1
• RefSeq Size: 2882 bp
• RefSeq ORF: 1401 bp
• Locus ID: 6663
• Cytogenetics: 22q13.1
• Protein Families: Adult stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Gene Summary: 'This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]'