Tropomyosin 3 (TPM3) (NM_153649) Human Untagged Clone

CAT#: SC111042

TPM3 (untagged)-Human tropomyosin 3 (TPM3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPM3
• Synonyms: CAPM1; CFTD; HEL-189; HEL-S-82p; hscp30; NEM1; OK/SW-cl.5; TM-5; TM3; TM5; TM30; TM30nm; TPM3nu; TPMsk3; TRK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111042 sequence for NM_153649 edited (data generated by NextGen Sequencing)
  ATGGCTGGGATCACCACCATCGAGGCGGTGAAGCGCAAGATCCAGGTTCTGCAGCAGCAG
  GCAGATGATGCAGAGGAGCGAGCTGAGCGCCTCCAGCGAGAAGTTGAGGGAGAAAGGCGG
  GCCCGGGAACAGGCTGAGGCTGAGGTGGCCTCCTTGAACCGTAGGATCCAGCTGGTTGAA
  GAAGAGCTGGACCGTGCTCAGGAGCGCCTGGCCACTGCCCTGCAAAAGCTGGAAGAAGCT
  GAAAAAGCTGCTGATGAGAGTGAGAGAGGTATGAAGGTTATTGAAAACCGGGCCTTAAAA
  GATGAAGAAAAGATGGAACTCCAGGAAATCCAACTCAAAGAAGCTAAGCACATTGCAGAA
  GAGGCAGATAGGAAGTATGAAGAGGTGGCTCGTAAGTTGGTGATCATTGAAGGAGACTTG
  GAACGCACAGAGGAACGAGCTGAGCTGGCAGAGTCCCGTTGCCGAGAGATGGATGAGCAG
  ATTAGACTGATGGACCAGAACCTGAAGTGTCTGAGTGCTGCTGAAGAAAAGTACTCTCAA
  AAAGAAGATAAATATGAGGAAGAAATCAATATTCTTACTGATAAACTCAAGGAGGCAGAG
  ACCCGTGCTGAGTTTGCTGAGAGATCGGTAGCCAAGCTGGAAAAGACAATTGATGACCTG
  GAAGATAAACTGAAATGCACCAAAGAGGAGCACCTCTGTACACAAAGGATGCTGGACCAG
  ACCCTGCTTGACCTAAATGAGATGTAG
  
  Clone variation with respect to NM_153649.3
  570 g=>t;735 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153649 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCAACGCCGAGCGG
  AGGAGGCAGGAACCGGAGCGCGAGCAGTAGCTGGGTGGGCACCATGGCTGGGATCACCAC
  CATCGAGGCGGTGAAGCGCAAGATCCAGGTTCTGCAGCAGCAGGCAGATGATGCAGAGGA
  GCGAGCTGAGCGCCTCCAGCGAGAAGTTGAGGGAGAAAGGCGGGCCCGGGAACAGGCTGA
  GGCTGAGGTGGCCTCCTTGAACCGTAGGATCCAGCTGGTTGAAGAAGAGCTGGACCGTGC
  TCAGGAGCGCCTGGCCACTGCCCTGCAAAAGCTGGAAGAAGCTGAAAAAGCTGCTGATGA
  GAGTGAGAGAGGTATGAAGGTTATTGAAAACCGGGCCTTAAAAGATGAAGAAAAGATGGA
  ACTCCAGGAAATCCAACTCAAAGAAGCTAAGCACATTGCAGAAGAGGCAGATAGGAAGTA
  TGAAGAGGTGGCTCGTAAGTTGGTGATCATTGAAGGAGACTTGGAACGCACAGAGGAACG
  AGCTGAGCTGGCAGAGTCCCGTTGCCGAGAGATGGATGAGCAGATTAGACTGATGGACCA
  GAACCTGAAGTGTCTGAGTGCTGCTGAAGAAAAGTACTCTCAAAAAGAAGATAAATATGA
  GGAAGAAATCAATATTCTTACTGATAAACTCAAGGAGGCAGAGACCCGTGCTGAGTTTGC
  TGAGAGATCGGTAGCCAAGCTGGAAAAGACAATTGATGACCTGGAAGATGAGCTCTATGC
  CCAGAAAACTGAGTACANGGCCATTAGCGAGGAGCTGGACCACGCNNCTCATGACATGNA
  CCTCTATATAACTGGAATGCACCAAAGAGGAGCACCTCTGTACACAAANATGCTGGACCA
  GACCCTGCTGACCTAATGAGATGTAGACGCCCCAGTCCACCTGCTGCTGCTCTCCTCTGA
  CCANNACTCGCTGAGCANCTGCGGAGCTGACTTACTGAGGCTGATCTTACTGGAGCTGCT
  TCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153649 unedited
  NNNNGGGTATTAGCTAGNAACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTT
  TTGGAGGGACTGGTTCTTTATTTCAAAAAGACACTTGTCAATATTCAGTATCAAAACAGT
  TGCACTATTGATTTCTCTTTCTCCCAATCGGCCCCAAAGAGACCACATAAAAGGAGAGTA
  CATTTTAAGCCAATAAGCTGCAGGATGTACACCTAACAGACCTCCTAGAAACCTTACCAG
  AAAATGGGGACTGGGTAGGGAAGGAAACTTAAAAGATCAACAAACTGCCAGCCCACGGAC
  TGCAGAGGCTGTCACAGCCAGATGGGGTGGCCAGGGTGCCACAAACCCAAAGCAAAGTTT
  CAAAATAATATAAAATTTAAAAAGTTTTGTACATAAGCTATTCAAGATTTCTCCAGCACT
  GACTGATACAAAGCACAATTGAGATGGCACTTCTAGAGACAGCAGCTTCAAACCCAGAAA
  AGGGTAATGAGATGAGTTTCACATGGCTAAATCAGTGGCAAAAACACAGTCTTCTTTCTT
  TCTTTCTTTCAAGGAGGCAGGAAAGCAATTAAGTGGTCACCTCAACATAAGGGGGACATG
  ATCCATTCTGTAAGCAGTTGTGAGGGGGTAGAGATGGGACAAAATTTTGGTCTCAGAGGT
  CTTACCATCTTAATTTGGTAACTTCTCATGAAAAAAATAAAAAATAGAAATAACCTTTTC
  CAAAGATATCTTAAAGCTGAAAACTTGAACAGCACATTTTNTGGGTTTTGGTGGNTGGTT
  GGCTAACTCCTCCTGGGATCACCTTTCTGGGTTTAGCTAGTACTTTGTACAGAACAATGG
  AGTTTCCCACAGCGGAATCTCCCTGGGCTCTGGTTTGCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153649
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153649.2, NP_705935.1
• RefSeq Size: 2080 bp
• RefSeq ORF: 747 bp
• Locus ID: 7170
• Cytogenetics: 1q21.3
• Domains: Tropomyosin
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Pathways in cancer, Thyroid cancer
• Gene Summary: 'This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]'
  Transcript Variant: This variant (Tpm3.1, also known as variant 2) encodes isoform Tpm3.1cy (also known as Tm5NM1, TM-5, isoform 2, or TM30nm). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.