UBE2N (NM_003348) Human Untagged Clone

CAT#: SC111050

UBE2N (untagged)-Human ubiquitin-conjugating enzyme E2N (UBE2N)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2N
• Synonyms: HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003348 edited
  GAATTCGGCACGAGGGTTCTGACAAGATGGCCGGGCTGCCCCGCAGGATCATCAAGGAAA
  CCCAGCGTTTGCTGGCAGAACCAGTTCCTGGCATCAAAGCCGAACCAGATGAGAGCAACG
  CCCGTTATTTTCATGTGGTCATTGCTGGCCCTCAGGATTCCCCCTTTGAGGGAGGGACTT
  TTAAACTTGAACTATTCCTTCCAGAAGAATACCCAATGGCAGCCCCTAAAGTACGTTTCA
  TGACCAAAATTTATCATCCTAATGTAGACAAGTTGGGAAGAATATGTTTAGATATTTTGA
  AAGATAAGTGGTCCCCAGCACTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGT
  TAAGTGCTCCCAATCCAGATGATCCATTAGCAAATGATGTAGCGGAGCAGTGGAAGACCA
  ACGAAGCCCAAGCCATAGAAACAGCTAGAGCATGGACTAGGCTATATGCCATGAATAATA
  TTTAAATTGATACGATCATCAAGTGTGCATCACTTCTCCTGTTCTGCCAAGACTTCCTCC
  TCTTTGTTTGCATTTAATGGACACAGTCTTAGAAACATTACAGAATAAAAAAGCCCAGAC
  ATCTTCAGTCCTTTGGTGATTAAATGCACATTAGCAAATCTATGTCTTGXXXXXXXXXXX
  XXXXXCTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003348 unedited
  TTAGATTTTGTAAACGACTTATATAGGCGGCCGACGAATTCGCACGAGGGTTCTGACAAG
  ATGGCCGGGCTGCCCCGCAGGATCATCAAGGAAACCCAGCGTTTGCTGGCAGAACCAGTT
  CCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCCGTTATTTTCATGTGGTCATTGCT
  GGCCCTCAGGATTCCCCCTTTGAGGGAGGGACTTTTAAACTTGAACTATTCCTTCCAGAA
  GAATACCCAATGGCAGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTA
  GACAAGTTGGGAAGAATATGTTTAGATATTTTGAAAGATAAGTGGTCCCCAGCACTGCAG
  ATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAGTGCTCCCAATCCAGATGATCCA
  TTAGCAAATGATGTAGCGGAGCAGTGGAAGACCAACGAAGCCCAAGCCATAGAAACAGCT
  AGAGCATGGACTAGGCTATATGCCATGAATAATATTTAAATTGATACGATCATCAAGTGT
  GCATCACTTCTCCTGTTCTGCCAAGACTTCCTCCTCTTTGTTTGCATTTAATGGACACAG
  TCTTAGAAACATTACAGAATAAAAAAGCCCAGACATCTTCAGTCCTTTGGTGATTAAATG
  CACATTAGCAAATCTATGTCTTGTCCTGATTCACTGTCATANAGCATGAGCAGAGGCTAG
  AAGTATCATCTGGATTGNTGTGAAAACGTTAAAGCAGTGGCCCCTCCCTGCTTTTATTCA
  TTTCCCCCATCCTGGTTTAAGTATAAAGCACTGTGAATGAAAGTAGTTGTCAGGTTAGCT
  GCAAGGNGTGTGGNNGNTNNTATTTAATTTATTTTATTTAATTTTGAGGGGGGAGTAGTT
  ATATTTTAAGGGCCCCCTTTCCCCCTTTTTGGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003348 unedited
  GGCAACTTTCCAGGGCCAGGAATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTAGAATAACAAAAAATATTTTACTAAAACATAAGATTTACAGAAGTTTCCAGACAAGC
  CATACAAAATGGTCACAAGCTTTTTTTGAAGGGGGGAATCTACACTTGACAGCAATGTTA
  TTAGTGAGGGCTGTGATGTTTGTTTAATGTTCCCATTTTGGTTCCAACAATCAAGCTTGT
  CCATCTACAGCGTCTAAATAAAGTTAGACTTGGCTAGAGCATATTCTAAAGACCTGGTTA
  GCTGCTTTTAACCAATGCAATTAGATCACCAAAAAAGGGGGAAAGGAGCCCATAAAATTA
  AACTACCTCCCCCCTCAAAAATAAAATAAAATAAAATAAAATAAAAACACCCACACCCCT
  GCAGCTAACCTGACAACTACCTTCATTCACAGTGCTTTATACTTAAACCAGGATGGGGGA
  AATGAATAAAAGCAGGGAGGGGCCACTGCTTTTAAACGTTTCACAACAATCCAGATGATA
  CTTCTAGCCTCTGCTCATGCTTTATGACAGTGAATCAGGACAAGACATAGATTTGCTAAT
  GTGCATTTAATCACCAAAGGACTGAAGATGTCTGGGCTTTTTTATTCTGTAATGTTTCTA
  AGACTGTGTCCATTAAATGCAAACAAAGAGGNAGGAAGTCTTGGCAGACAGGNAGAGTGA
  TGCCACTTNGATGATCGTATCATTTAAATATATCATGCATATAGCCTAGTCCATGCTCTA
  CTGTTCTATGGCTGGGGCTCNNTTGGTCTTCACTGCTCGCTACATATTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003348
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003348.3, NP_003339.1
• RefSeq Size: 2568 bp
• RefSeq ORF: 459 bp
• Locus ID: 7334
• Cytogenetics: 12q22
• Domains: UBCc
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]'