Vimentin (VIM) (NM_003380) Human Untagged Clone

CAT#: SC111054

VIM (untagged)-Human vimentin (VIM)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VIM
• Synonyms: CTRCT30; HEL113
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111054 sequence for NM_003380 edited (data generated by NextGen Sequencing)
  ATGTCCACCAGGTCCGTGTCCTCGTCCTCCTACCGCAGGATGTTCGGCGGCCCGGGCACC
  GCGAGCCGGCCGAGCTCCAGCCGGAGCTACGTGACTACGTCCACCCGCACCTACAGCCTG
  GGCAGCGCGCTGCGCCCCAGCACCAGCCGCAGCCTCTACGCCTCGTCCCCGGGCGGCGTG
  TATGCCACGCGCTCCTCTGCCGTGCGCCTGCGGAGCAGCGTGCCCGGGGTGCGGCTCCTG
  CAGGACTCGGTGGACTTCTCGCTGGCCGACGCCATCAACACCGAGTTCAAGAACACCCGC
  ACCAACGAGAAGGTGGAGCTGCAGGAGCTGAATGACCGCTTCGCCAACTACATCGACAAG
  GTGCGCTTCCTGGAGCAGCAGAATAAGATCCTGCTGGCCGAGCTCGAGCAGCTCAAGGGC
  CAAGGCAAGTCGCGCCTGGGGGACCTCTACGAGGAGGAGATGCGGGAGCTGCGCCGGCAG
  GTGGACCAGCTAACCAACGACAAAGCCCGCGTCGAGGTGGAGCGCGACAACCTGGCCGAG
  GACATCATGCGCCTCCGGGAGAAATTGCAGGAGGAGATGCTTCAGAGAGAGGAAGCCGAA
  AACACCCTGCAATCTTTCAGACAGGATGTTGACAATGCGTCTCTGGCACGTCTTGACCTT
  GAACGCAAAGTGGAATCTTTGCAAGAAGAGATTGCCTTTTTGAAGAAACTCCACGAAGAG
  GAAATCCAGGAGCTGCAGGCTCAGATTCAGGAACAGCATGTCCAAATCGATGTGGATGTT
  TCCAAGCCTGACCTCACGGCTGCCCTGCGTGACGTACGTCAGCAATATGAAAGTGTGGCT
  GCCAAGAACCTGCAGGAGGCAGAAGAATGGTACAAATCCAAGTTTGCTGACCTCTCTGAG
  GCTGCCAACCGGAACAATGACGCCCTGCGCCAGGCAAAGCAGGAGTCCACTGAGTACCGG
  AGACAGGTGCAGTCCCTCACCTGTGAAGTGGATGCCCTTAAAGGAACCAATGAGTCCCTG
  GAACGCCAGATGCGTGAAATGGAAGAGAACTTTGCCGTTGAAGCTGCTAACTACCAAGAC
  ACTATTGGCCGCCTGCAGGATGAGATTCAGAATATGAAGGAGGAAATGGCTCGTCACCTT
  CGTGAATACCAAGACCTGCTCAATGTTAAGATGGCCCTTGACATTGAGATTGCCACCTAC
  AGGAAGCTGCTGGAAGGCGAGGAGAGCAGGATTTCTCTGCCTCTTCCAAACTTTTCCTCC
  CTGAACCTGAGGGAAACTAATCTGGATTCACTCCCTCTGGTTGATACCCACTCAAAAAGG
  ACACTTCTGATTAAGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAG
  CATCACGATGACCTTGAATAA
  
  Clone variation with respect to NM_003380.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003380 unedited
  GGTTTAGGATTTGTAACCGACTTACTATAGGGNCGGCACGCGCAATTCGGCACGAGGCTC
  CGAGGTCCCCGCGCCAGAGACGCAGCCGCGCTCCCACCACCCACACCCACCGCGCCCTCG
  TTCGCCTCTTCTCCGGGAGCCAGTCCGCGCCACCGCCGCCGCCCAGGCCATCGCCACCCT
  CCGCAGCCATGTCCACCAGGTCCGTGTCCTCGTCCTCCTACCGCAGGATGTTCGGCGGCC
  CGGGCACCGCGAGCCGGCCGAGCTCCAGCCGGAGCTACGTGACTACGTCCACCCGCACCT
  ACAGCCTGGGCAGCGCGCTGCGCCCCAGCACCAGCCGCAGCCTCTACGCCTCGTCCCCGG
  GCGGCGTGTATGCCACGCGCTCCTCTGCCGTGCGCCTGCGGAGCAGCGTGCCCGGGGTGC
  GGCTCCTGCAGGACTCGGTGGACTTCTCGCTGGCCGACGCCATCAACACCGAGTTCAAGA
  ACACCCGCACCAACGAGAAGGTGGAGCTGCAGGAGCTGAATGACCGCTTCGCCAACTACA
  TCGACAAGGTGCGCTTCCTGGAGCAGCAGAATAAGATCCTGCTGGCCGAGCTCGAGCAGC
  TCAAGGGCCAAGGCAAGTCGCGCCTGGGGGACCTCTACGAGGAGGAGATGCGGGAGCTGC
  GCCGGCAGGTGGACCAGCTAACCAACGANCAAGCCCGCGTCGAGGTGGAGCGCGANCACC
  TGGNCCGAGACATCATGCGCCTNCGGGAGAAATTGCAGGANGAGATGCTTCAGAGAGAGG
  AAGCCGAAACACCCTGCAATCTTTCAGACAGGGATGTGACNATGCGTCTCTGGCACGTCT
  TGACCTTGAACGCAAAGTGGAATCTTTGCAAGAAGAGATTGCCCCTTTTGAAGAAACTCC
  NCCGAAAGGAAATCCAGGAGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003380 unedited
  ATGGACCGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAGTTTTTCCAA
  AGATTATTGAAGCAGAACCAAGTTGGTTGGATACTTGCTGGAAAAAAAAACAGTTTTAAT
  GGTATTCAAAATACCTTTTAAAAAGTATTCTAGCACAAGATTTTTCTGTAAACTAGATTA
  TGTTGTAAACTTTTTTCTAAATCTTGTAGGAGTGTCGGTTGTTAAGAACTAGAGCTTATT
  CCTATTCCAAATCTATCTTGCGCTCCTGAAAAACTGCAGAAAGGCACTTGAAAGCTGTTT
  CTTTAAGATATGGATTTCTTTTTTATTCTTGCTGGTAATATATTGCTGCACTGAGTGTGT
  GCAATTTTTATTCAAGGTCATCGTGATGCTGAGAAGTTTCGTTGATAACCTGTCCATCTC
  TAGTTTCAACCGTCTTAATCAGAAGTGTCCTTTTTGAGTGGGTATCAACCAGAGGGAGTG
  AATCCAGATTAGTTTCCCTCAGGTTCAGGGAGGAAAAGTTTGGAAGAGGCAGAGAAATCC
  TGCTCTCCTCGCCTTCCAGCAGCTTCCTGTAGGTGGCAATCTCAATGTCAAGGGCCATCT
  TAACATTGAGCAGGTCTTGGTATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCT
  GAATCTCATCCTGCAGGCGGCCAATAGTGTCTTGGTAGTTAGCAGCTTCAACGGCAAAGT
  TCTCTTCCATTTCACGCATCTGGCGTTCCAGGAACTCATTGGTTCCTTTAGGGCCTCCAC
  TCACAGGTGAAGGACTGCACCTGTCTCCGGACTTAGTGGACTCCTGCCTTGCCTGCCGCA
  AGCGCGTATTGTTCCGGTTGGCAACCTAAAGAGTCAGCAACTCGGATTTGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003380
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003380.2, NP_003371.2
• RefSeq Size: 1847 bp
• RefSeq ORF: 1401 bp
• Locus ID: 7431
• Cytogenetics: 10p13
• Domains: filament, filament_head
• Protein Families: ES Cell Differentiation/IPS
• Gene Summary: 'This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]'