ELL (NM_006532) Human Untagged Clone

CAT#: SC111065

ELL (untagged)-Human elongation factor RNA polymerase II (ELL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELL
• Synonyms: C19orf17; ELL1; MEN; PPP1R68
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111065 sequence for NM_006532 edited (data generated by NextGen Sequencing)
  ATGGCGGCGCTGAAGGAGGATAGGAGCTACGGGCTGTCGTGCGGGCGGGTTAGCGACGGC
  AGCAAGGTGTCGGTGTTCCACGTGAAGCTCACCGACAGTGCCCTGAGGGCCTTCGAGAGC
  TACCGCGCCAGACAGGATTCTGTTTCACTGAGGCCATCTATCCGATTTCAAGGAAGCCAA
  GGGCACATCTCCATCCCCCAGCCTGACTGCCCCGCAGAGGCGCGGACGTTCTCCTTCTAC
  CTCTCCAACATCGGCCGCGACAACCCCCAGGGCAGCTTCGACTGCATCCAGCAGTATGTC
  TCCAGTCATGGGGAAGTTCACCTGGACTGCCTGGGCAGCATACAGGACAAGATCACGGTG
  TGTGCCACCGACGACTCCTACCAGAAGGCGCGGCAGAGCATGGCCCAGGCGGAGGAGGAG
  ACGCGGAGCCGAAGTGCCATTGTCATCAAGGCTGGAGGCCGCTACCTGGGCAAGAAGGTT
  CAGTTTCGGAAACCAGCCCCAGGTGCAACAGACGCGGTGCCCTCCCGGAAGCGGGCAACC
  CCCATCAACTTGGCGAGTGCCATCAGGAAGAGTGGTGCCAGTGCCGTGAGTGGGGGCAGC
  GGGGTGTCCCAGAGGCCCTTCCGTGACCGAGTGCTGCACCTCCTGGCACTACGGCCCTAC
  CGCAAGGCTGAGCTGCTGCTGCGACTGCAGAAGGACGGCCTGACGCAGGCGGACAAGGAC
  GCGCTGGATGGCCTCCTCCAGCAGGTGGCCAACATGAGTGCTAAGGACGGCACGTGTACA
  CTGCAGGACTGCATGTACAAGGATGTGCAGAAGGACTGGCCTGGCTACTCGGAGGGGGAC
  CAGCAGCTGCTGAAGCGGGTGCTCGTCCGGAAGCTGTGCCAGCCACAGAGCACTGGCAGC
  CTCCTTGGAGACCCTGCTGCCTCCAGCCCCCCAGGCGAGCGTGGGCGCTCGGCCTCGCCC
  CCACAGAAGCGGCTGCAGCCTCCTGATTTCATCGACCCCCTAGCCAACAAGAAACCCCGG
  ATATCGCACTTCACTCAGAGAGCTCAGCCTGCCGTCAACGGGAAGCTGGGCGTGCCCAAT
  GGCCGTGAGGCCTTGCTGCCCACCCCGGGCCCACCAGCCAGCACGGACACCCTCAGCTCC
  AGCACTCACCTGCCCCCGCGGCTGGAGCCCCCGAGGGCCCACGACCCCCTGGCCGATGTC
  AGCAATGACCTGGGCCACAGCAGCCGAGACTGTGAGCACGGAGAGGCGGCTGCCCCAGCC
  CCCACTGTGCGCCTCGGCCTGCCCCTGCTGACGGACTGTGCCCAGCCCAGCAGGCCACAC
  GGCAGCCCCTCGCGCAGCAAGCCCAAGAAGAAGTCCAAGAAGCACAAAGACAAGGAGAGG
  GCGGCTGAGGACAAGCCCCGGGCCCAGCTTCCAGACTGTGCACCTGCCACCCATGCCACC
  CCCGGAGCCCCAGCAGACACCCCAGGTTTAAACGGAACCTGCAGCGTTTCCAGTGTTCCC
  ACGTCCACGTCGGAGACGCCTGACTACTTGCTGAAGTACGCAGCCATCTCCTCTTCGGAG
  CAGCGCCAGAGCTACAAGAACGACTTCAATGCCGAGTACAGCGAGTACCGCGACCTGCAC
  GCCCGCATTGAGCGCATCACGCGGCGGTTCACCCAGCTCGACGCCCAGCTCCGGCAGCTC
  TCCCAGGGCTCCGAGGAGTATGAGACTACTCGAGGGCAGATTTTGCAGGAATATCGAAAA
  ATCAAAAAGACCAACACCAACTACAGCCAGGAGAAGCACCGCTGCGAGTACCTGCACAGC
  AAGCTGGCCCACATCAAGAGGCTCATCGCCGAGTACGACCAGCGGCAGCTGCAGGCTTGG
  CCCTAG
  
  Clone variation with respect to NM_006532.3
  1222 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006532 unedited
  CCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
  TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC
  GGCACGAGGCGCAAGATGGCGGCGCTGAAGGAGGATAGGAGCTACGGGCTGTCGTGCGGG
  CGGGTTAGCGACGGCAGCAAGGTGTCGGTGTTCCACGTGAAGCTCACCGACAGTGCCCTG
  AGGGCCTTCGAGAGCTACCGCGCCAGACAGGATTCTGTTTCACTGAGGCCATCTATCCGA
  TTTCAAGGAAGCCAAGGGCACATCTCCATCCCCCAGCCTGACTGCCCCGCAGAGGCGCGG
  ACGTTCTCCTTCTACCTCTCCAACATCGGCCGCGACAACCCCCAGGGCAGCTTCGACTGC
  ATCCAGCAGTATGTCTCCAGTCATGGGGAAGTTCACCTGGACTGCCTGGGCAGCATACAG
  GACAAGATCACGGTGTGTGCCACCGACGACTCCTACCAGAAGGCGCGGCAGAGCATGGCC
  CAGGCGGAGGAGGAGACGCGGAGCCGAAGTGCCATTGTCATCAAGGCTGGAGGCCGCTAC
  CTGGGCAAGAAGGTTCAGTTTCGGAAACCAGCCCCAGGTGCAACAGACGCGGTGCCCTCC
  CGGAAGCGGGCAACCCCCATCAACTTGGCGAGTGCCATCANGAAGAGTGGTGCCAGTGCC
  GTGAGTGGGGGCAGCGGGGTGTCCCAGAAGCCCTTCCGTGACCGAGTGCTGCACCTCCTG
  GCACTACNGCCCTACCGCAAGGCTGAGCTGCTGCTGCGACTGCAGAAGGACNGCCTGACG
  CANGCNNGACAGGACGCGCTGGATGGCCNTCTCCCAGCAGTGGCCAACATGAGTGCTAAG
  GACNGCACGTGTACACTGGCAGACTGCATGTACAAGGATGTGCANAAGGACTGGCCCTGN
  CTACTCGNAGGGGACANNCACTGCTGAANCGGNNTGCTCGTCCGAAGCTGTGCCAGCACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006532 unedited
  CTANGTTCNGTTTTTTTTTTTTTTTTTTTTAACAAAAAATTTAACCCTTTTTAAACAACT
  TAGAACTTTGGCCCCCAACCCCCAAAACAAAAAAATTTTCTTAACTTCATAATTCCCTAA
  AAGGGGAACCAATTAACCACCAAAACCACCACCACCACCAAAAGGGCAATTCAACTGGCC
  ACCCCTTGGACCGGTTCCCAAGGCAAAAGGGCCGGCAATCCCGGGCTGGGACCCCCCGAA
  ATCATCCCACCCTACCCGGGTTTTGAAGGCTTCCCGGGGGCCAAACAACAAGGGCCAACT
  TACCTTGGAAGCCCCCTGGAACCCGGTTGGGCCTTTCCCGGGCCGGCCCGGGCCAAAAAA
  AAAAACCACCACCGCTTTTGGAACCTTGAAGATCCATTTCCCCGGAACCTTGGGAACCCC
  GGGGGCCCAAAAGAAAAAAACCCTTGGGCCAGGGGGGGATACCCCGGGGGCCCCCCACAA
  TCAACTCTTTGGAAACCTGCAAGAAGCCCCCCTCCACCCCCCAATTTAAAAAAATAAACA
  TCTGGTTTTTTGCCTTTTTGGTCCCCCCGGCCGACTGGTTGTGGCAACCATGACCCCCAT
  GGGATCCCTGGGCCCTGGATTACAAGCCGCATTCAACCCCCAACAACTTTCCAAATCATG
  GGTTCATTCAACCCTTAATTGGAACCCCCACCCTTAAACCCCACCCTGCCCTGGGGGGGT
  CCCCAAATAACCCAACTACACAACCACAATTTGGTTGCCCCCCGATGCCTGGGGGGGACC
  CTAGACTAGGGCCACAGCACCGAACCACCATTGGGGTGGCCTGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006532
• ORF Size: 1866 bp
• Insert Size: 4000
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_006532.2, NP_006523.1
• RefSeq Size: 4058
• RefSeq ORF: 1866
• Locus ID: 8178
• Protein Families: Transcription Factors
• Gene Summary: Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation. [UniProtKB/Swiss-Prot Function]