DGKE (NM_003647) Human Untagged Clone

CAT#: SC111075

DGKE (untagged)-Human diacylglycerol kinase, epsilon 64kDa (DGKE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DGKE
• Synonyms: AHUS7; DAGK5; DAGK6; DGK; NPHS7
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003647 edited
  GCGTCGTTCTCCTCCTGCGCGAGGCGGCCAAGGCCTGCTGGCCCGGAGCCGCGCCTCCAC
  CCGCGCGAGGTATCGTCCTTGGAGAAGATGGAAGCGGAGAGGCGGCCGGCGCCGGGCTCG
  CCCTCCGAGGGCCTGTTTGCGGACGGGCACCTGATCTTGTGGACGCTGTGCTCGGTCCTG
  CTGCCGGTGTTCATCACCTTCTGGTGTAGCCTCCAGCGGTCGCGCCGGCAGCTGCACCGC
  AGGGACATCTTCCGCAAGAGCAAGCACGGATGGCGCGACACGGACCTGTTCAGCCAGCCC
  ACCTACTGCTGCGTGTGCGCGCAGCACATTCTGCAGGGCGCCTTCTGCGACTGCTGCGGG
  CTCCGCGTGGACGAGGGCTGCCTCAGGAAGGCCGACAAGCGCTTCCAGTGCAAGGAGATT
  ATGCTCAAGAATGACACCAAGGTCCTGGACGCCATGCCCCACCACTGGATCCGGGGCAAC
  GTGCCGCTGTGCAGTTACTGTATGGTTTGCAAGCAGCAGTGTGGCTGTCAACCCAAGCTT
  TGCGATTACAGGTGCATTTGGTGCCAGAAAACAGTACATGATGAGTGCATGAAAAATAGT
  TTAAAGAATGAAAAATGTGATTTTGGAGAATTCAAAAACCTAATCATTCCACCAAGTTAT
  TTAACATCCATTAATCAGATGCGTAAAGACAAAAAAACAGATTATGAAGTGCTAGCCTCT
  AAGCTTGGAAAGCAGTGGACCCCATTAATAATCCTGGCCAACTCTCGTAGTGGAACTAAT
  ATGGGAGAAGGACTGTTGGGAGAATTTAGGATCTTGTTGAATCCAGTCCAGGTTTTTGAT
  GTAACTAAAACTCCTCCTATCAAAGCCCTACAACTCTGTACTCTTCTCCCATATTATTCA
  GCTCGAGTACTTGTTTGTGGAGGGGATGGGACTGTAGGGTGGGTCCTGGATGCAGTTGAT
  GACATGAAGATTAAGGGACAAGAAAAGTACATTCCACAAGTTGCAGTTTTGCCTCTGGGA
  ACAGGCAACGATCTATCCAATACATTGGGTTGGGGTACAGGTTATGCTGGAGAAATTCCA
  GTTGCGCAGGTTTTGCGAAATGTAATGGAAGCAGATGGAATTAAACTAGATCGATGGAAA
  GTTCAAGTAACAAATAAAGGATACTACAACTTAAGAAAACCCAAGGAATTCACAATGAAC
  AACTATTTTTCTGTTGGACCTGATGCTCTCATGGCTCTCAATTTTCATGCTCATCGTGAG
  AAGGCACCATCTCTGTTTTCTAGCAGAATTCTTAATAAGGCGGTTTACTTATTCTATGGA
  ACCAAAGATTGTTTAGTGCAAGAATGTAAAGATTTGAATAAAAAAGTTGAGCTAGAACTG
  GATGGTGAGCGAGTAGCACTGCCCAGCTTGGAAGGTATTATAGTTCTGAACATCGGATAC
  TGGGGCGGTGGCTGCAGACTATGGGAAGGGATGGGGGACGAGACTTACCCTCTAGCCAGG
  CATGACGATGGTCTGCTGGAAGTCGTTGGAGTATATGGGTCTTTCCACTGTGCTCAGATT
  CAAGTAAAACTGGCTAATCCTTTTCGAATAGGACAGGCACATACAGTGAGGCTGATTTTG
  AAGTGCTCCATGATGCCAATGCAGGTGGATGGGGAGCCTTGGGCCCAAGGGCCCTGCACT
  GTCACCATAACTCACAAGACACATGCAATGATGTTATATTTCTCTGGAGAACAAACAGAT
  GATGACATCTCTAGTACTTCGGATCAAGAAGATATAAAGGCGACTGAATAGATGGATGAG
  GGAGTGAAAACTTTGCATAGAATCCTCACGCAAGTAGATACATGTTCATCCAAAAGTATT
  AATAGAAATTCTCTATCAGCTATTCAGTCTT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003647 unedited
  TTATAACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGGCTTCATCCCTGCTGGCGGCCCAGCGTCGTTCTCCTCCTGCGC
  GAGGCGGCCAAGGCCTGCTGGCCCGGAGCCGCGCCTCCACCCGCGCGAGGTATCGTCCTT
  GGAGAAGATGGAAGCGGAGAGGCGGCCGGCGCCGGGCTCGCCCTCCGAGGGCCTGTTTGC
  GGACGGGCACCTGATCTTGTGGACGCTGTGCTCGGTCCTGCTGCCGGTGTTCATCACCTT
  CTGGTGTAGCCTCCAGCGGTCGCGCCGGCAGCTGCACCGCAGGGACATCTTCCGCAAGAG
  CAAGCACGGATGGCGCGACACGGACCTGTTCAGCCAGCCCACCTACTGCTGCGTGTGCGC
  GCAGCACATTCTGCAGGGCGCCTTCTGCGACTGCTGCGGGCTCCGCGTGGACGAGGGCTG
  CCTCAGGAAGGCCGACAAGCGCTTCCAGTGCAAGGAGATTATGCTCAAGAATGACACCAA
  GGTCCTGGACGCCATGCCCCACCACTGGATCCGGNGCAACGTGCCGCTGTGCAGTTACTG
  TATGGTTTGCAAGCAGCAGTGTGGCTGTCAACCCAAGCTTTGCGATTACAGGTGCATTTG
  GNTGCCAGAAACAGTACATGATGAGTGCATGANANATAGTTAANAGAAATGAAAATGTGA
  TTTTGGNAGAAATCANAACCCTATCATTCCACCAGTTATTAACATCCATTATCAGATGCG
  NTAAGACAAAAAACAGATTATGAAGTGCTAGCCTCTAAGCTGGAAAGCAGTGGACCCCAT
  TNATATNCTGGNCACTCTCGTAGTGGACTATATGGGAGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003647 unedited
  NNNNNNCCGGTTTAGCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTT
  TTTTTAAATATCTAGATATTTTAAATATATCTACTTATATTCCACTGTCCTCAACTGGGT
  AGAAATATTAAAAAAATTATCAATATAAAATGCTGGTTCTTGCTTCAATATTATCATTTT
  GAAGGAAAACCACACAAAACCATTACCCATTCTAATGACTTTTTTGGCCAAATTATGTAT
  TTGGGTCCACATTAATCATTAAATTAGTATAACCTTTAAAGTTTTCAAGTTAGTGCAAAT
  TTAAAATTCAATAAATATATTAAATACAATTTTACTTTATATTACAAATGTAAATTATTT
  TTCTTTTGACATGCTGGTAGTATTCATTTTTACAACGCAAACTGACTTTACAATTTCTGA
  GTCACAGTATATTTTATAACTCTAAGCAAATGTATTTCACAAAGCAAACACTATATGTTT
  TTGACATACTCTAAAAAAATAAAAATAAATAATTTTGATTATAAAATTTGTATAAACTTA
  GCTACATTAGGGGTTCACAGCTAATTGTTGCAGAAAACCTAAAACACACCTAAATCATGT
  AACTTTACTAGCAGACAACTTGTATTTTAAAACACAAGTCAGTTTGATTTCTATTAAACA
  TGACGAAACACTGAAAATTATCATCATCTGCCTAAGGGATCTTTAAGAGCTGCCAGGTAT
  TCAGTATTTAAGCCACTACGTTTATAGGAATTAGGAATGTATTGGGATAGTAAAAAGATT
  AGAGAAATCTGGAAACAGACATGGAGATATGATTTACTTCATGTATTTCTTTCCTGAGGA
  TGTAGAAGTTATTTTTTTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003647
• ORF Size: 1704 bp
• Insert Size: 4500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003647.1, NP_003638.1
• RefSeq Size: 2562
• RefSeq ORF: 1704
• Locus ID: 8526
• Domains: DAGKa, DAGKc, DAG_PE-bind
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]