CLN3 (NM_000086) Human Untagged Clone

CAT#: SC111112

CLN3 (untagged)-Human ceroid-lipofuscinosis, neuronal 3 (CLN3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLN3
• Synonyms: BTN1; BTS; JNCL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111112 sequence for NM_000086 edited (data generated by NextGen Sequencing)
  ATGGGAGGCTGTGCAGGCTCGCGGCGGCGCTTTTCGGATTCCGAGGGGGAGGAGACCGTC
  CCGGAGCCCCGGCTCCCTCTGTTGGACCATCAGGGCGCGCATTGGAAGAACGCGGTGGGC
  TTCTGGCTGCTGGGCCTTTGCAACAACTTCTCTTATGTGGTGATGCTGAGTGCCGCCCAC
  GACATCCTTAGCCACAAGAGGACATCGGGAAACCAGAGCCATGTGGACCCAGGCCCAACG
  CCGATCCCCCACAACAGCTCATCACGATTTGACTGCAACTCTGTCTCTACGGCTGCTGTG
  CTCCTGGCGGACATCCTCCCCACACTCGTCATCAAATTGTTGGCTCCTCTTGGCCTTCAC
  CTGCTGCCCTACAGCCCCCGGGTTCTCGTCAGTGGGATTTGTGCTGCTGGAAGCTTCGTC
  CTGGTTGCCTTTTCTCATTCTGTGGGGACCAGCCTGTGTGGTGTGGTCTTCGCTAGCATC
  TCATCAGGCCTTGGGGAGGTCACCTTCCTCTCCCTCACTGCCTTCTACCCCAGGGCCGTG
  ATCTCCTGGTGGTCCTCAGGGACTGGGGGAGCTGGGCTGCTGGGGGCCCTGTCCTACCTG
  GGCCTCACCCAGGCCGGCCTCTCCCCTCAGCAGACCCTGCTGTCCATGCTGGGTATCCCT
  GCCCTGCTGCTGGCCAGCTATTTCTTGTTGCTCACATCTCCTGAGGCCCAGGACCCTGGA
  GGGGAAGAAGAAGCAGAGAGCGCAGCCCGGCAGCCCCTCATAAGAACCGAGGCCCCGGAG
  TCGAAGCCAGGCTCCAGCTCCAGCCTCTCCCTTCGGGAAAGGTGGACAGTGTTCAAGGGT
  CTGCTGTGGTACATTGTTCCCTTGGTCGTAGTTTACTTTGCCGAGTATTTCATTAACCAG
  GGACTTTTTGAACTCCTCTTTTTCTGGAACACTTCCCTGAGTCACGCTCAGCAATACCGC
  TGGTACCAGATGCTGTACCAGGCTGGCGTCTTTGCCTCCCGCTCTTCTCTCCGCTGCTGT
  CGCATCCGTTTCACCTGGGCCCTGGCCCTGCTGCAGTGCCTCAACCTGGTGTTCCTGCTG
  GCAGACGTGTGGTTCGGCTTTCTGCCAAGCATCTACCTCGTCTTCCTGATCATTCTGTAT
  GAGGGGCTCCTGGGAGGCGCAGCCTACGTGAACACCTTCCACAACATCGCCCTGGAGACC
  AGTGATGAGCACCGGGAGTTTGCAATGGCGGCCACCTGCATCTCTGACACACTGGGGATC
  TCCCTGTCGGGGCTCCTGGCTTTGCCTCTGCATGACTTCCTCTGCCAGCTCTCCTGA
  
  Clone variation with respect to NM_000086.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000086 unedited
  NGCGTTCAGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTTAAAG
  ACCCTCATCCCTCCCGTGGGAGCCCCCTTTGGACACTCTATGACCCTGGACCCTCGGGGG
  ACCTGAACTTGATGCGATGGGAGGCTGTGCAGGCTCGCGGCGGCGCTTTTCGGATTCCGA
  GGGGGAGGAGACCGTCCCGGAGCCCCGGCTCCCTCTGTTGGACCATCAGGGCGCGCATTG
  GAAGAACGCGGTGGGCTTCTGGCTGCTGGGCCTTTGCAACAACTTCTCTTATGTGGTGAT
  GCTGAGTGCCGCCCACGACATCCTTAGCCACAAGAGGACATCGGGAAACCAGAGCCATGT
  GGACCCAGGCCCAACGCCGATCCCCCACAACAGCTCATCACGATTTGACTGCAACTCTGT
  CTCTACGGCTGCTGTGCTCCTGGCGGACATCCTCCCCACACTCGTCATCAAATTGTTGGC
  TCCTCTTGGCCTTCACCTGCTGCCCTACAGCCCCCGGGTTCTCGTCAGTGGGATTTGTGC
  TGCTGGAAGCTTCGTCCTGGTTGCCTTTTCTCATTCTGTGGGGACCAGCCTGTGTGGTGT
  GGTCTTCGCTAGCATCTCATCAGGCCTTGGGGAGGTCACCTTCCTCTCCCTCACTGCCTT
  CTACCCCAGGGCCGTGATCTCCTGGTGGTCCTCAGGGACTGGGGGAGCTGGGCTGCTGGG
  GGCCCTGTCCTACCTGGGCCTCACCCAGGCCGGCCTCTCCCCTCAGCAGACCCTGCTGTC
  CATGCTGGGTATCCCTGCCCTGCTGCTGGCCAGCTATTTCTTGTTGCTCACATCTCCTGA
  GGCCCANGACCCNTGNAGGGAAGAAGAAGCAGAGAGCGCAGCCCGGCAGCCCCTCATAAG
  AACCCGAGCCCCGGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000086 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATGGATAAAATCGGCATTTATTCA
  GAAGGCATGATGCCAGGAAGAACTCCCCAGTGGGAGAAATGGCTGGCCCCCCTGCAAGGG
  AAACAAGGCTTCAGCCCTTCCCTACTCCCAAACCTGCCGGGAAGGCTGGGAGCACAGTTC
  ATGGAGGGTCTCTGGGGTGGGCCTGGGTGTCTGACCTGTCCCTCTGCCCACAGGTGAATG
  TGACCTGCGTCCTGAGGATCCCGAGTATCAGGAGAGCTGGCAGAGGAAGTCATGCAGAGG
  CAAAGCCAGGAGCCCCGACAGGGAGATCCCCAGTGTGTCAGATATGCAGGTGGCCGCCAT
  TGCAAACTCCCGGAGCTCATCACTGGTCTCCAGGGCGATGTTGTGGAAGGTGTTCACGTA
  GGCTGCGCCTCCCAGGAGCCCCTCATACAGAATGATCAGGAAGACGAGGTAGATGCTTGG
  CAGATAGCCGAACCACACGTCTGCCAGCAGGAACACCAGGTTGAGGCACTGCAGCAGGGC
  CAGGGCCCAGGTGAAACGGATGCGACAGCAGCGGAGAGAAGAGCGGGAGGCAAAGACGCC
  AGCCTGGACAGCATCTGGTACCAGCGTTTTGCTGAGCGTGACTCAGGGAAGTGCTCCAGA
  TAAAGAGGAGTTCAAAAAGCCCCTGCCTTATTGAAATACTCGGCAAGTAAACTACGACCC
  AAAGGAACAATGTCCCACAGCAGACCCTTGAACACTGTCCACCTTTTCCGNATGGAGATG
  CTGAACCTTGAACCTGCCTTCACTCCGGGGCCCTGGGTCTTATGAAGGGCTGCCGGCTGA
  GCTCTTTGTTTTTCTCCCTNCAGGGTCCGGGCCTAAGAAATGGGACAACAGAAATTCTGT
  CCCCACAGGCAAGGATTCACACATGACACAGGTCGCTAAGGGAAGCCGCCTGGTGAGCCA
  ATTTGAAGGCCCCAAACCATTCCCGACCTGGACCAAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000086
• Insert Size: 1870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000086.1, NP_000077.1
• RefSeq Size: 1689 bp
• RefSeq ORF: 1317 bp
• Locus ID: 1201
• Cytogenetics: 16p12.1
• Domains: CLN3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: 'This gene encodes a protein that is involved in lysosomal function. Mutations in this, as well as other neuronal ceroid-lipofuscinosis (CLN) genes, cause neurodegenerative diseases commonly known as Batten disease or collectively known as neuronal ceroid lipofuscinoses (NCLs). Many alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (a).