UHRF1 (NM_013282) Human Untagged Clone

CAT#: SC111252

UHRF1 (untagged)-Human ubiquitin-like with PHD and ring finger domains 1 (UHRF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UHRF1
• Synonyms: hNP95; hUHRF1; huNp95; ICBP90; Np95; RNF106; TDRD22
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013282, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGGTTTTTGCTGTCCCTCCCCTCAGCGCCGACACCATGTGGATCCAGGTTCGGACCATGGACGGGA
  GGCAGACCCACACGGTGGACTCGCTGTCCAGGCTGACCAAGGTGGAGGAGCTGAGGCGGAAGATCCAGGA
  GCTGTTCCACGTGGAGCCAGGCCTGCAGAGGCTGTTCTACAGGGGCAAACAGATGGAGGACGGCCATACC
  CTCTTCGACTACGAGGTCCGCCTGAATGACACCATCCAGCTCCTGGTCCGCCAGAGCCTCGTGCTCCCCC
  ACAGCACCAAGGAGCGGGACTCCGAGCTCTCCGACACCGACTCCGGCTGCTGCCTGGGCCAGAGTGAGTC
  AGACAAGTCCTCCACCCACGGTGAGGCGGCCGCCGAGACTGACAGCAGGCCAGCCGATGAGGACATGTGG
  GATGAGACGGAATTGGGGCTGTACAAGGTCAATGAGTACGTCGATGCTCGGGACACGAACATGGGGGCGT
  GGTTTGAGGCGCAGGTGGTCAGGGTGACGCGGAAGGCCCCCTCCCGGGACGAGCCCTGCAGCTCCACGTC
  CAGGCCGGCGCTGGAGGAGGACGTCATTTACCACGTGAAATACGACGACTACCCGGAGAACGGCGTGGTC
  CAGATGAACTCCAGGGACGTCCGAGCGCGCGCCCGCACCATCATCAAGTGGCAGGACCTGGAGGTGGGCC
  AGGTGGTCATGCTCAACTACAACCCCGACAACCCCAAGGAGCGGGGCTTCTGGTACGACGCGGAGATCTC
  CAGGAAGCGCGAGACCAGGACGGCGCGGGAACTCTACGCCAACGTGGTGCTGGGGGATGATTCTCTGAAC
  GACTGTCGGATCATCTTCGTGGACGAAGTCTTCAAGATTGAGCGGCCGGGTGAAGGGAGCCCCATGGTTG
  ACAACCCCATGAGACGGAAGAGCGGGCCGTCCTGCAAGCACTGCAAGGACGACGTGAACAGACTCTGCCG
  GGTCTGCGCCTGCCACCTGTGCGGGGGCCGGCAGGACCCCGACAAGCAGCTCATGTGCGATGAGTGCGAC
  ATGGCCTTCCACATCTACTGCCTGGACCCGCCCCTCAGCAGTGTTCCCAGCGAGGACGAGTGGTACTGCC
  CTGAGTGCCGGAATGATGCCAGCGAGGTGGTACTGGCGGGAGAGCGGCTGAGAGAGAGCAAGAAGAAGGC
  GAAGATGGCCTCGGCCACATCGTCCTCACAGCGGGACTGGGGCAAGGGCATGGCCTGTGTGGGCCGCACC
  AAGGAATGTACCATCGTCCCGTCCAACCACTACGGACCCATCCCGGGGATCCCCGTGGGCACCATGTGGC
  GGTTCCGAGTCCAGGTCAGCGAGTCGGGTGTCCATCGGCCCCACGTGGCTGGCATACACGGCCGGAGCAA
  CGACGGAGCGTACTCCCTAGTCCTGGCGGGGGGCTATGAGGATGATGTGGACCATGGGAATTTTTTCACA
  TACACGGGTAGTGGTGGTCGAGATCTTTCCGGCAACAAGAGGACCGCGGAACAGTCTTGTGATCAGAAAC
  TCACCAACACCAACAGGGCGCTGGCTCTCAACTGCTTTGCTCCCATCAATGACCAAGAAGGGGCCGAGGC
  CAAGGACTGGCGGTCGGGGAAGCCGGTCAGGGTGGTGCGCAATGTCAAGGGTGGCAAGAATAGCAAGTAC
  GCCCCCGCTGAGGGCAACCGCTACGATGGCATCTACAAGGTTGTGAAATACTGGCCCGAGAAGGGGAAGT
  CCGGGTTTCTCGTGTGGCGCTACCTTCTGCGGAGGGACGATGATGAGCCTGGCCCTTGGACGAAGGAGGG
  GAAGGACCGGATCAAGAAGCTGGGGCTGACCATGCAGTATCCAGAAGGCTACCTGGAAGCCCTGGCCAAC
  CGAGAGCGAGAGAAGGAGAACAGCAAGAGGGAGGAGGAGGAGCAGCAGGAGGGGGGCTTCGCGTCCCCCA
  GGACGGGCAAGGGCAAGTGGAAGCGGAAGTCGGCAGGAGGTGGCCCGAGCAGGGCCGGGTCCCCGCGCCG
  GACATCCAAGAAAACCAAGGTGGAGCCCTACAGTCTCACGGCCCAGCAGAGCAGCCTCATCAGAGAGGAC
  AAGAGCAACGCCAAGCTGTGGAATGAGGTCCTGGCGTCACTCAAGGACCGGCCGGCGAGCGGCAGCCCGT
  TCCAGTTGTTCCTGAGTAAAGTGGAGGAGACGTTCCAGTGTATCTGCTGTCAGGAGCTGGTGTTCCGGCC
  CATCACGACCGTGTGCCAGCACAACGTGTGCAAGGACTGCCTGGACAGATCCTTTCGGGCACAGGTGTTC
  AGCTGCCCTGCCTGCCGCTACGACCTGGGCCGCAGCTATGCCATGCAGGTGAACCAGCCTCTGCAGACCG
  TCCTCAACCAGCTCTTCCCCGGCTACGGCAATGGCCGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013282 unedited
  NGGTCAGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCAGCGTT
  TGCCGAGCGGGCGCTCCGGGTCGCACGCAAGTCCGCGCGGGGTCCGGGCCACGCACGCGG
  TTTCATCGCCATCCCCAGCCGGGCCACGCGCGCAGGCAGACAAGCTGTTCGCGGCGACCG
  GAGAGCGCCGACACCATGTGGATCCAGGTTCGGACCATGGACGGGAGGCAGACCCACACG
  GTGGACTCGCTGTCCAGGCTGACCAAGGTGGAGGAGCTGAGGCGGAAGATCCAGGAGCTG
  TTCCACGTGGAGCCAGGCCTGCAGAGGCTGTTCTACAGGGGCAAACAGATGGAGGACGGC
  CATACCCTCTTCGACTACGAGGTCCGCCTGAATGACACCATCCAGCTCCTGGTCCGCCAG
  AGCCTCGTGCTCCCCCACAGCACCAAGGAGCGGGACTCCGAGCTCTCCGACACCGACTCC
  GGCTGCTGCCTGGGCCAGAGTGAGTCAGACAAGTCCTCCACCCACGGTGAGGCGGCCGCC
  GAGACTGACAGCAGGCCAGCCGATGAGGACATGTGGGATGAGACGGAATTGGGGCTGTAC
  AAGGTCAATGAGTACGTCGATGCTCGGGACACGAACATGNGGGGCGTGGTTTGAGGCGCA
  NGTGGTCAGGGTGACGCGGAAGGCCCCCTCCCGGGACGAGCCCTGCAGCTCCACGTCCAG
  GCCGGCGCTGGNAGGAGACGTCATTTACCACGTGAAATACGACGACTACCCGGAGAACCG
  GCGTGGTCCAGATGAACTCCAGGGACGTNCGAGCGCGCGCCCGCACCATCATCAAGTGGC
  AGGACCTGGNAGTGGGCCCAGNTGGTCATGCTCAACTACAACCCCGGAAACCNCAAGGAG
  CGGGGGCTTTTGGTAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013282 unedited
  NNNTTTTGACTCTGGACCCGCGGCCGCATNCTAANGATCGGTTTTTTTTTTTTTTTTTTT
  TTTTTTTATCTGGGAAAAACTTTATTCTGAGAATCTAAAATCTGGACAAAGTACTGGACT
  TTAAAAAAAGCCTACACAAAATTGTCTCATTCTTCCCTAATACATTAATAATCTAAGAAT
  AAGGAGGTGAAAAAAACCCTTTAAAAATAACATTGCTCCAGTTTGTCTGCAGGTATGTGA
  TTTAAAATATCCCTGTTTTATTGAGGTATAGGCTGCAAACTTTGGTAAAATTAGAAAAAT
  TAACAAACCCTTTCAAAAGAAAAAAAATACAAAAACAAAAACAACCAAACCCTAACGTTA
  TATCTTTCTTGGTACATATACAGTAACTACAAAAAAAATTCATTTAAAAAATGTGTTCAT
  TAAGAGAAAAAAAAATTTTAAAAAAACCCTCTTGTAAGTTCTCACTTGCTGAGTTAAAAA
  AATTGAATGTAAATGATCTATAAGGCTCAAACGGTTCCCAAGCACCCATCCGGTGTCAGG
  AAGAACTGGATGACCGCAGTTTCTAGAATCTGGTTGACATTTTCAATAATTCAGTGCTAA
  CAACAAAGACTGGTCGTCTTTAGAAAGAAGGAACGAATCAAAGGCACTTGCACGTGACTT
  TCGTGGCCTGGCCGTCCGGTCCGTTGGCCGCCTTCGTCGGACAGNGTCAGTCACACGCCC
  TGGCCCCACGTGGAGCAAAATGCCCTTGGAGGCATTTTTTGGGGGGCCCCACCTCCCTGT
  TTTCGTGGGGCAAGGACTTTCACCGCGGATCTTAGACGCTAACAGCCTGTGGGGGGGAGA
  GGCTTTAGGGACAAGGGTTAAACCTCGCCCCAAAAGTTTCCCTTTTTCCTTGGAAAAATT
  TTAAAAAAATTGAACCCCCCCTGCCCGGGCTTTCCCGGCCACCCAGGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013282
• ORF Size: 2382 bp
• Insert Size: 3740
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_013282.2, NP_037414.2
• RefSeq Size: 3828
• RefSeq ORF: 2382
• Locus ID: 29128
• Domains: UBQ, RING, PHD, SRA
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation from an alternate start codon, compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.