APH1A (NM_016022) Human Untagged Clone

CAT#: SC111265

APH1A (untagged)-Human anterior pharynx defective 1 homolog A (C. elegans) (APH1A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APH1A
• Synonyms: 6530402N02Rik; APH-1; APH-1A; CGI-78
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016022, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGGCTGCGGTGTTTTTCGGCTGCACTTTCGTCGCGTTCGGCCCGGCCTTCGCGCTTTTCTTGATCA
  CTGTGGCTGGGGACCCGCTTCGCGTTATCATCCTGGTCGCAGGGGCATTTTTCTGGCTGGTCTCCCTGCT
  CCTGGCCTCTGTGGTCTGGTTCATCTTGGTCCATGTGACCGACCGGTCAGATGCCCGGCTCCAGTACGGC
  CTCCTGATTTTTGGTGCTGCTGTCTCTGTCCTTCTACAGGAGGTGTTCCGCTTTGCCTACTACAAGCTGC
  TTAAGAAGGCAGATGAGGGGTTAGCATCGCTGAGTGAGGACGGAAGATCACCCATCTCCATCCGCCAGAT
  GGCCTATGTTTCTGGTCTCTCCTTCGGTATCATCAGTGGTGTCTTCTCTGTTATCAATATTTTGGCTGAT
  GCACTTGGGCCAGGTGTGGTTGGGATCCATGGAGACTCACCCTATTACTTCCTGACTTCAGCCTTTCTGA
  CAGCAGCCATTATCCTGCTCCATACCTTTTGGGGAGTTGTGTTCTTTGATGCCTGTGAGAGGAGACGGTA
  CTGGGCTTTGGGCCTGGTGGTTGGGAGTCACCTACTGACATCGGGACTGACATTCCTGAACCCCTGGTAT
  GAGGCCAGCCTGCTGCCCATCTATGCAGTCACTGTTTCCATGGGGCTCTGGGCCTTCATCACAGCTGGAG
  GGTCCCTCCGAAGTATTCAGCGCAGCCTCTTGTGTAAGGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016022 unedited
  GCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTGACATTGCACCG
  CGCCCCTCGTGGGGTCGCGTTGCCACCCCACGCGGACTCCCCAGCTGGCGCGCCCCTCCC
  ATTTGCCTGTCCTGGTCAGGCCCCCACCCCCCTTCCCACCTGACCAGCCATGGGGGCTGC
  GGTGTTTTTCGGCTGCACTTTCGTCGCGTTCGGCCCGGCCTTCGCGCTTTTCTTGATCAC
  TGTGGCTGGGGACCCGCTTCGCGTTATCATCCTGGTCGCAGGGGCATTTTTCTGGCTGGT
  CTCCCTGCTCCTGGCCTCTGTGGTCTGGTTCATCTTGGTCCATGTGACCGACCGGTCAGA
  TGCCCGGCTCCAGTACGGCCTCCTGATTTTTGGTGCTGCTGTCTCTGTCCTTCTACAGGA
  GGTGTTCCGCTTTGCCTACTACAAGCTGCTTAAGAAGGCAGATGAGGGGTTAGCATCGCT
  GAGTGAGGACGGAAGATCACCCATCTCCATCCGCCAGATGGCCTATGTTTCTGGTCTCTC
  CTTCGGTATCATCAGTGGTGTCTTCTCTGTTATCAATATTTTGGCTGATGCACTTGGGCC
  AGGTGTGGTTGGGATCCATGGAGACTCACCCTATTACTTCCTGACTTCAGCCTTTCTGAC
  AGCAGCCATTATCCTGCTCCATACCTTTTGGGGAGTTGTGTTCTTTGATGCCTGTGAGAG
  GAGACGGTACTGNGCTNTGGGCCTGGNTGTTTGGGAGTCACCTACTGACATCGGGACTGA
  CATTCCTGAACCCCTGGTATGANGCCAGCCTGCTGCCCATCTATGCAGTCACTGGTTCCA
  TGGGGCTCTGGGCCTTCATCACAGCTGGAGGGTCCCTTCGAAGTATCAGCGCAGCCTCTG
  TGCCGCGNCAGNAGACAGTCGNGGAATGGGGTATCTGCCTGCGCTCCACCGAGGCTGAGA
  ACCCTAGGGGGACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016022 unedited
  AAAGAACCTGGNGGGNAAATAAAGCCCATGTTCACATGCACCTTGGTATAAAGGGAGGGG
  AACTCCACCCTCAGTAGAAAAGTTACAGTTTATAGAAAACCTCCCCGCCCCTCCCACACC
  CCAATTAAAAACTACAAAAAAAATCTCCCCTCCTTCCCTACGATGCCATGGCAGTCTGAC
  TCCTCCAGTGGCACTGCAGCTCTGGTAGTGGCCAGCTCACCACAGCACCCTCCACTTTAC
  CTCGTCGACACGAGGGATGCTGGTGGCTAAGGAGGTTAAAACCACCACTCCCAGCACTGC
  CAGCCCCCAAACATGCACTTCACTTCCTTTCCCCCAAGGTCTGGGACCAAGGATAAAGGG
  ACCCAAATGAGCCCACCCATGACGCACCAACCCCAGGTGAGAAAACATAGCCCCACGACG
  CTAGAACCAGCTCCAATATACCTTCTCCCTCCCACCACCTCAAAAATTATAACAGGCAGT
  TTACGGTATTTATCACACCAAAAATGGCCCCCCCTCACGCCTGCCAAAACGACCCGCACG
  TCCACCCTTCAGGAATACTGAAAAACTCAAACCTCATTATCCTTTCAGCCCCCATCAATC
  CCATCTCCAGCGCCACGGCTCCTCCTACGTCCCCTTTTTCTTGGCCATCCTGCCCCTGTC
  CATAACTGGCCACTGGCCAACAATCATGGGCGATGGCCATTATGAATCGCACCTCCAGTC
  CCCAGGGGCCCCCCACTTTAGTNTCCCCTCTACTCTCCTCCTTCTGCCTCCCTCCTGGCC
  TTCATTCACCCCTCACCCCCTCGTGTCCCTCTCCGTCGGCGGGCACCCATAAGGCCGTCG
  CCGAAATATCTCCCGACACGACCCCTCCCAACGTCTCCTAGAAGGTCCCACATGCTCCCT
  GTCTAACACGGTAATCGCCGTTAAATCGCCCTCCCACACTCGCCCACTCTAACCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016022
• ORF Size: 756 bp
• Insert Size: 1800
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_016022.1, NP_057106.1
• RefSeq Size: 2290
• RefSeq ORF: 756
• Locus ID: 51107
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Alzheimer's disease, Notch signaling pathway
• Gene Summary: This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (2) contains an alternate segement in the 3' terminus that results in a frameshift and premature stop codon, compared to variant 1, and encodes an isoform (2; also known as isoform S) with a shorter and distinct C-terminus, compared to isoform 1.