Angiopoietin 1 (ANGPT1) (NM_001146) Human Untagged Clone

CAT#: SC111587

ANGPT1 (untagged)-Human angiopoietin 1 (ANGPT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANGPT1
• Synonyms: AGP1; AGPT; ANG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111587 sequence for NM_001146 edited (data generated by NextGen Sequencing)
  ATGACAGTTTTCCTTTCCTTTGCTTTCCTCGCTGCCATTCTGACTCACATAGGGTGCAGC
  AATCAGCGCCGAAGTCCAGAAAACAGTGGGAGAAGATATAACCGGATTCAACATGGGCAA
  TGTGCCTACACTTTCATTCTTCCAGAACACGATGGCAACTGTCGTGAGAGTACGACAGAC
  CAGTACAACACAAACGCTCTGCAGAGAGATGCTCCACACGTGGAACCGGATTTCTCTTCC
  CAGAAACTTCAACATCTGGAACATGTGATGGAAAATTATACTCAGTGGCTGCAAAAACTT
  GAGAATTACATTGTGGAAAACATGAAGTCGGAGATGGCCCAGATACAGCAGAATGCAGTT
  CAGAACCACACGGCTACCATGCTGGAGATAGGAACCAGCCTCCTCTCTCAGACTGCAGAG
  CAGACCAGAAAGCTGACAGATGTTGAGACCCAGGTACTAAATCAAACTTCTCGACTTGAG
  ATACAGCTGCTGGAGAATTCATTATCCACCTACAAGCTAGAGAAGCAACTTCTTCAACAG
  ACAAATGAAATCTTGAAGATCCATGAAAAAAACAGTTTATTAGAACATAAAATCTTAGAA
  ATGGAAGGAAAACACAAGGAAGAGTTGGACACCTTAAAGGAAGAGAAAGAGAACCTTCAA
  GGCTTGGTTACTCGTCAAACATATATAATCCAGGAGCTGGAAAAGCAATTAAACAGAGCT
  ACCACCAACAACAGTGTCCTTCAGAAGCAGCAACTGGAGCTGATGGACACAGTCCACAAC
  CTTGTCAATCTTTGCACTAAAGAAGGTGTTTTACTAAAGGGAGGAAAAAGAGAGGAAGAG
  AAACCATTTAGAGACTGTGCAGATGTATATCAAGCTGGTTTTAATAAAAGTGGAATCTAC
  ACTATTTATATTAATAATATGCCAGAACCCAAAAAGGTGTTTTGCAATATGGATGTCAAT
  GGGGGAGGTTGGACTGTAATACAACATCGTGAAGATGGAAGTCTAGATTTCCAAAGAGGC
  TGGAAGGAATATAAAATGGGTTTTGGAAATCCCTCCGGTGAATATTGGCTGGGGAATGAG
  TTTATTTTTGCCATTACCAGTCAGAGGCAGTACATGCTAAGAATTGAGTTAATGGACTGG
  GAAGGGAACCGAGCCTATTCACAGTATGACAGATTCCACATAGGAAATGAAAAGCAAAAC
  TATAGGTTGTATTTAAAAGGTCACACTGGGACAGCAGGAAAACAGAGCAGCCTGATCTTA
  CACGGTGCTGATTTCAGCACTAAAGATGCTGATAATGACAACTGTATGTGCAAATGTGCC
  CTCATGTTAACAGGAGGATGGTGGTTTGATGCTTGTGGCCCCTCCAATCTAAATGGAATG
  TTCTATACTGCGGGACAAAACCATGGAAAACTGAATGGGATAAAGTGGCACTACTTCAAA
  GGGCCCAGTTACTCCTTACGTTCCACAACTATGATGATTCGACCTTTAGATTTTTGA
  
  Clone variation with respect to NM_001146.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001146 unedited
  NGGGTGCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAATATG
  TACACGCAGCTGACTCAGGCAGGCTCCATGCTGAACGGTCACACAGAGAGGAAACAATAA
  ATCTCAGCTACTATGCAATAAATATCTCAAGTTTTAACGAAGAAAAACATCATTGCAGTG
  AAATAAAAAATTTTAAAATTTTAGAACAAAGCTAACAAATGGCTAGTTTTCTATGATTCT
  TCTTCAAACGCTTTCTTTGAGGGGGAAAGAGTCAAACAAACAAGCAGTTTTACCTGAAAT
  AAAGAACTAGTTTTAGAGGTCAGAAGAAAGGAGCAAGTTTTGCGAGAGGCACGGAAGGAG
  TGTGCTGGCAGTACAATGACAGTTTTCCTTTCCTTTGCTTTCCTCGCTGCCATTCTGACT
  CACATAGGGTGCAGCAATCAGCGCCGAAGTCCAGAAAACAGTGGGAGAAGATATAACCGG
  ATTCAACATGGGCAATGTGCCTACACTTTCATTCTTCCAGAACACGATGGCAACTGTCGT
  GAGAGTACGACAGACCAGTACAACACAAACGCTCTGCAGAGAGATGCTCCACACGTGGAA
  CCGGATTTCTCTTCCCAGAAACTTCAACATCTGGAACATGTGATGGAAAATTATACTCAG
  TGGCTGCAAAAACTTGAGAATTACATTGTGGAAAACATGAAGTCGGAGATGGCCCAGATA
  CAGCAGAATGCAGTTCAGAACCACACGGCTACCATGCTGGAGATAGGAACCAGCCTNCTC
  TCTCAGACTGCAGAGCAGACCANAAAGCTGACAGATGTTGAGACCCAAGTACTAAAATCA
  ACTNCTCGACTTGAGATACAGCTGCTGGAGGATTCATTATCCCACCTACAGCTAGAAAGC
  AACTTCTTCACAGACAATGAAATCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001146 unedited
  ATATCTNTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTGGAATATGAGTTTGGAAATAGTATTCTTTATCCAAAAACTGGTTTAAGAAAGTGTCT
  GTAGGAACACAAAAGGACAAAATACTCATTTTCTTTCCCTTAAATGAAAACAGTTGAGAA
  GTTTTTAGAAGGAGAGGCTTACCTGCTATGAAGAAGCCACCAAGAATAGAAAAATGGTAT
  AATACCTGCAAATATCACTTGTAAGGCAAAAAGGGGTAGAATACAGACACCTAAATATAA
  AAAAGACTTGATGGTAAACTTTAAAAAGTATTTTTCTTAACAATGTGAATAGCTTTATTT
  TCTCAAATGGAGGAAACCATTAAGGCATAGTGGATCAAGTCACCAAGTTTGAAACTGGTA
  TTGCTACCTTGCCAACAACTGTCTCTTTTATGAGAGGAGGCCCAGTAGCTTTATTTCAAT
  TTTCTCATCCCATAGTGACTCATGTTTTAATAAAAACTGTTTACTGTCATAACCTTGGAC
  ACTTACAGGCAGAGAAGACTTCTTGATAAAATAATGCAGTAACTTCAAGCACCCCAAAAA
  TTCACTTAAAATACATTATCCCATAGGAATATTTCCATTGAGTTATTTATGGCAACTAGC
  AGTCAGAATTTCTTAGTCAGGTGACTATGATTAATTTTCTTTTTGTAATATTTCCCTTAC
  TATAATAGTTTTGCCAATTTTTCTCCCCTGAAATAATTTTTAAAATTAAAAATTGCCTAA
  AGGAATGGACTGAAGGAGAAATTATGAAGAATGATGTTTTTCATTAAGGAATATAGTTAG
  ATTTCTTTGAGCATTTGTGGTTTTTTGCATCAATTAAAGACAATGGTTGCCTATTCCCAG
  ATAATTAGCTATTAAGGTTTTTTGCAAATATAAACATGCTATGTACAATGATATTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001146
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146.3, NP_001137.2
• RefSeq Size: 4338 bp
• RefSeq ORF: 1497 bp
• Locus ID: 284
• Cytogenetics: 8q23.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Gene Summary: 'This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Mutations in this gene are associated with hereditary angioedema. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.